SASDBX4: Albumen gland precursor of perivitellin-2 (PcPV2) (Perivitellin-2...

Basic information

• Entry: Database: SASBDB / ID: SASDBX4

Sample

Albumen gland precursor of perivitellin-2 (PcPV2)

• Perivitellin-2 31 kDa subunit (protein), PcPV2-31, Pomacea canaliculata
• Perivitellin-2 67 kDa subunit (protein), PcPV2-67, Pomacea canaliculata

Function / homology

Function:

nutrient reservoir activity / toxin activity / carbohydrate binding / extracellular region / membrane

Domain/homology:

Propeller / Tectonin domain / Beta-propeller repeat TECPR / Beta propeller repeats in Physarum polycephalum tectonins, Limulus lectin L-6 and animal hypothetical proteins. / Membrane attack complex component/perforin domain, conserved site / Membrane attack complex/perforin (MACPF) domain signature. / Membrane attack complex/perforin (MACPF) domain profile. / MAC/Perforin domain / Membrane attack complex component/perforin (MACPF) domain

Component:

Perivitellin-2 67 kDa subunit / Perivitellin-2 31 kDa subunit

• Biological species: Pomacea canaliculata (invertebrata)
• Citation: Journal: Physiol Biochem Zool / Year: 2017; Title: Apple Snail Perivitellin Precursor Properties Help Explain Predators' Feeding Behavior.; Authors: María Pilar Cadierno / Marcos Sebastián Dreon / Horacio Heras / ; Abstract: In contrast with vitellogenin maturation, it is unknown whether gastropod perivitellin precursors are subject to large structural changes. The gastropod reproductive tract includes an accessory organ, the albumen gland (AG), that produces and secretes perivitelline fluid. In the apple snail Pomacea canaliculata, the large, reddish-pink AG provides eggs with perivitellins that are defensive against predators. Although the AG makes a considerable contribution to apple snail biomass, field observations indicate that it is rejected by avian and mammalian predators, although the underlying reason remains unknown. By analyzing the structure-function properties of P. canaliculata perivitellin precursors, we provide insight into perivitellin maturation and its relationship with apple snail predator feeding behavior. Structural analysis using small-angle X-ray scattering, absorption and fluorescence spectroscopy, circular dichroism, electrophoresis, chromatography, and partial proteolysis showed that the size, shape, and structure of perivitellin precursors resemble those of egg mature forms. Functional analysis indicates that the precursors of the defensive perivitellins ovorubin (PcOvo) and perivitellin-2 (PcPV2) are highly stable and antinutritive, withstanding proteinase digestion and displaying structural stability of their quaternary structure under a wide pH range (4.0-10.0). Furthermore, AG extracts limit a predator's ability to digest nutrients and are toxic to mice (median lethal concentration 96 h after administration: 5.9 mg/kg). Treated mice displayed neurologic signs similar to those produced by egg PcPV2. Results indicate that apple snails store active precursors of egg proteins inside the AG, providing evidence that gastropod perivitellin precursors do not experience the large structural processing of invertebrate vitellogenin maturation. These defensive proteins provide the apple snail AG with neurotoxic, antinutritive, and antidigestive activity, a likely explanation for the predators' feeding behavior.

Contact author

• Marcos Dreon

Models

• Model #539: ; Type: dummy / Software: (r3695) / Radius of dummy atoms: 3.60 A / Chi-square value: 3.0976; Search similar-shape structures of this assembly by Omokage search (details)
• Model #540: ; Type: dummy / Software: (r3695) / Radius of dummy atoms: 3.60 A / Chi-square value: 3.010225; Search similar-shape structures of this assembly by Omokage search (details)

Sample

• Sample: Name: Albumen gland precursor of perivitellin-2 (PcPV2) / Specimen concentration: 0.60-2.40 / Entity id: 346 / 347
• Buffer: Name: 20 mM Tris-HCl / Concentration: 20.00 mM / pH: 8.5

Entity #346

Name: PcPV2-31 / Type: protein / Description: Perivitellin-2 31 kDa subunit / Formula weight: 31.584 / Num. of mol.: 4 / Source: Pomacea canaliculata / References: UniProt: P0C8G7
Sequence:

MVKKIHFIME RHASIVAFLL AVLALTESQA FTSVKLPRDE HWPYNYVSIG PAGVWAVNRQ NKLFYRTGTY GDNANMGSGW QFKQDGVGQV DVGKDKVGYI NLSGGSLFRI DGISQGNPVG GSPKSWEWWT KYIGMSLRED TRFSSRIENQ NKVLTFTFRT CFWASRITNW CFADSSYTET VTAGGSGTWI TKSQLKYKSG TFGNPDTEGG DWITVDSGSF QHVSSGSGVV LAVRSNGELV KRTGITCSLP QGSGWTSMLN GMSRVDTYGT VAWAVDTYGD LYFINL

Entity #347

Name: PcPV2-67 / Type: protein / Description: Perivitellin-2 67 kDa subunit / Formula weight: 62.517 / Num. of mol.: 4 / Source: Pomacea canaliculata / References: UniProt: P0C8G6
Sequence:

MSQLRWWVVS QLLLLIVVCI LDHSEGARVC PKIVPGLDKL RVGVDITKLD LLPLFDLGDN GFRSAVADYT CDRGQTTVVD GESFDVPDQV DSVVIESSGQ QTSSVTTIKS ESQISQALSI SAGISVDTAK AGFSSSASYA EMQEAITKYG RTVSQMSAVY TTCSANLSPN LLLGQNPLQT LSRLPSDFTA DTEGYYDFIK TYGTHYFNKG KLGGMFLFTS ETDMSYFQNK NSQQVEANIK ATFASILSTE TGGSSDQSKE VIEFKESSLI TAKFFGGRTN LAADGLTKWQ PTIAKLPYFM SGTLSTISSL IADTTKRASM ELAVKNYLLK AKVANLDRLT YIRLNSWTVG HNELRDLSAQ LQNLKKKTIF SDEDEKLLQS IEDQVSVPAW FSDRTTFCFR STAVGSADQC NGQSTSTLCA EPNRYTQQYM DKTYLGDTGC RLVWKLSTTE SSDWFKSVKV NFRWYPTWSP CACGPVGTPF TISAPANSWT QDYLDVTNPK FGECMLQWMI EVPPTATLWA KNLEFCIDFT CGKKKQCVDA NHWTEPYLDI SAHEACGMSW ALIAK

Experimental information

• Beam: Instrument name: Brazilian Synchrotron Light Laboratory SAXS2 Beamline; City: Campinas / 国: Brazil / Type of source: X-ray synchrotronSynchrotron
• Detector: Name: MAR 165 CCD

Scan

Title: Albumen gland precursor of perivitellin-2 (PcPV2) / Measurement date: Jun 10, 2014 / Storage temperature: 25 °C / Cell temperature: 25 °C / Exposure time: 300 sec. / Number of frames: 5 / Unit: 1/nm /

	Min	Max
Q	0.0846	2.0807

Distance distribution function P(R)

Sofotware P(R): GNOM 4.5a / Number of points: 178 /

	Min	Max
Q	0.1086	1.528
P(R) point	3	180
R	0	16.96

Result

Type of curve: single_conc /

	Experimental	Porod
MW	400 kDa	-
Volume	-	294 nm3

	P(R)	Guinier	P(R) error	Guinier error
Forward scattering, I0	0.0004835	0.00049	-	-
Radius of gyration, Rg	4.86 nm	4.84 nm	0.009	0.18

	Min	Max
D	-	16.96
Guinier point	3	20