SASDBS9: TET12SN (TET12(1.10)SN-f5)

基本情報

• 登録情報: データベース: SASBDB / ID: SASDBS9

試料

TET12SN

• TET12(1.10)SN-f5 (protein), TET12SN, synthetic construct

• 生物種: synthetic construct (人工物)
• 引用: ジャーナル: Nat Biotechnol / 年: 2017; タイトル: Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo.; 著者: Ajasja Ljubetič / Fabio Lapenta / Helena Gradišar / Igor Drobnak / Jana Aupič / Žiga Strmšek / Duško Lainšček / Iva Hafner-Bratkovič / Andreja Majerle / Nuša Krivec / Mojca Benčina / Tomaž Pisanski / Tanja Ćirković Veličković / Adam Round / José María Carazo / Roberto Melero / Roman Jerala / ; 要旨: Polypeptides and polynucleotides are natural programmable biopolymers that can self-assemble into complex tertiary structures. We describe a system analogous to designed DNA nanostructures in which protein coiled-coil (CC) dimers serve as building blocks for modular de novo design of polyhedral protein cages that efficiently self-assemble in vitro and in vivo. We produced and characterized >20 single-chain protein cages in three shapes-tetrahedron, four-sided pyramid, and triangular prism-with the largest containing >700 amino-acid residues and measuring 11 nm in diameter. Their stability and folding kinetics were similar to those of natural proteins. Solution small-angle X-ray scattering (SAXS), electron microscopy (EM), and biophysical analysis confirmed agreement of the expressed structures with the designs. We also demonstrated self-assembly of a tetrahedral structure in bacteria, mammalian cells, and mice without evidence of inflammation. A semi-automated computational design platform and a toolbox of CC building modules are provided to enable the design of protein cages in any polyhedral shape.

登録者

• Jana Aupic (National Institute of Chemistry - Slovenia, Ljubljana, Slovenia)

モデル

• モデル #1130: ; タイプ: atomic / ダミー原子の半径: 1.90 A / カイ2乗値: 1.20139637252; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: TET12SN / 試料濃度: 0.90-3.60
• バッファ: 名称: 20 mM Tris 150 mM NaCl 10% glycerol / pH: 7.5

要素 #588

名称: TET12SN / タイプ: protein / 記述: TET12(1.10)SN-f5 / 分子量: 53.41 / 分子数: 1 / 由来: synthetic construct
配列:

MLEEELKQLE EELQAIEEQL AQLQWKAQAR KEKLAQLKEK LSGPGSPEDE IQQLEEEISQ LEQKNSELKE KNQELKYGSG PGDIEQELER AKESIRRLEQ EVNQERSRMQ YLQTLLEKSG PGQLEDKVEE LLSKNYHLEN EVERLKKLVG SGPGLEEELK QLEEELQAIE EQLAQLQWKA QARKEKLAQL KEKLSGPGSP EDEIQQLEEK NSQLKQEISQ LEEKNQELKY GSGPGQLEDK VEELLSKNYH LENEVERLKK LVGSGPGSPE DKISQLKEKI QQLKQENQQL EEENSQLEYG SGPGSPEDEN SQLEEKISQL KQKNSELKEE IQQLEYGSGP GSPEDKISEL KEENQQLEQK IQQLKEENSQ LEYGSGPGDI EQELERAKES IRRLEQEVNQ ERSRMQYLQT LLEKSGPGSP EDKNSELKEE IQQLEEENQQ LEEKISELKY GLEHHHHHHH H

実験情報

• ビーム: 設備名称: ESRF BM29 / 地域: Grenoble / 国: France / 線源: X-ray synchrotron / 波長: 0.1 Å / スペクトロメータ・検出器間距離: 2.87 mm
• 検出器: 名称: Pilatus 1M

スキャン

タイトル: TET12SN / 測定日: 2015年10月28日 / 保管温度: 20 °C / セル温度: 20 °C / 照射時間: 1 sec. / フレーム数: 10 / 単位: 1/nm /

	Min	Max
Q	0.0692	4.9116

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 565 /

	Min	Max
Q	0.181468	2.82025
P(R) point	1	565
R	0	12

結果

カーブのタイプ: single_conc

	実験値	Standard	Standard error	Porod
分子量	53 kDa	51.2 kDa	0.2	62.9 kDa
体積	-	-	-	107 nm3

	P(R)	P(R) error	Guinier	Guinier error
前方散乱 I0	50	0.17	51.14	0.13
慣性半径, Rg	3.51 nm	0.01	3.52 nm	0.07

	Min	Max
D	-	12
Guinier point	7	65