+データを開く
-基本情報
登録情報 | データベース: SASBDB / ID: SASDAK8 |
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試料 | Dark state solution structure of Aureochrome1a- A´α-LOV-Jα
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生物種 | Phaeodactylum tricornutum (海洋性珪藻) |
引用 | ジャーナル: Structure / 年: 2016 タイトル: Structure of a Native-like Aureochrome 1a LOV Domain Dimer from Phaeodactylum tricornutum. 著者: Ankan Banerjee / Elena Herman / Tilman Kottke / Lars-Oliver Essen / 要旨: Light-oxygen-voltage (LOV) domains absorb blue light for mediating various biological responses in all three domains of life. Aureochromes from stramenopile algae represent a subfamily of ...Light-oxygen-voltage (LOV) domains absorb blue light for mediating various biological responses in all three domains of life. Aureochromes from stramenopile algae represent a subfamily of photoreceptors that differs by its inversed topology with a C-terminal LOV sensor and an N-terminal effector (basic region leucine zipper, bZIP) domain. We crystallized the LOV domain including its flanking helices, A'α and Jα, of aureochrome 1a from Phaeodactylum tricornutum in the dark state and solved the structure at 2.8 Å resolution. Both flanking helices contribute to the interface of the native-like dimer. Small-angle X-ray scattering shows light-induced conformational changes limited to the dimeric envelope as well as increased flexibility in the lit state for the flanking helices. These rearrangements are considered to be crucial for the formation of the light-activated dimer. Finally, the LOV domain of the class 2 aureochrome PtAUREO2 was shown to lack a chromophore because of steric hindrance caused by M301. |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-モデル
モデル #324 | タイプ: dummy / ソフトウェア: DAMMIF / ダミー原子の半径: 3.25 A コメント: The model is an averaged and volume corrected representation (DAMFILT) カイ2乗値: 0.595 / P-value: 0.000373 Omokage検索でこの集合体の類似形状データを探す (詳細) |
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-試料
試料 | 名称: Dark state solution structure of Aureochrome1a- A´α-LOV-Jα 試料濃度: 0.75-15.00 |
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バッファ | 名称: Tris / 濃度: 10.00 mM / pH: 8 / 組成: 300 mM NaCl |
要素 #191 | 名称: Aureochrome1a-A / タイプ: protein / 記述: Aureochrome1a-A´α-LOV-Jα / 分子量: 17.973 / 分子数: 2 / 由来: Phaeodactylum tricornutum 配列: MGSSHHHHHH SSGLVPRGSH MDFSFIKALQ TAQQNFVVTD PSLPDNPIVY ASQGFLNLTG YSLDQILGRN CRFLQGPETD PKAVERIRKA IEQGNDMSVC LLNYRVDGTT FWNQFFIAAL RDAGGNVTNF VGVQCKVSDQ YAATVTKQQE EEEEAAANDD ED |
-実験情報
ビーム | 設備名称: ESRF BM29 / 地域: Grenoble / 国: France / 線源: X-ray synchrotron / 波長: 0.93 Å / スペクトロメータ・検出器間距離: 2.43 mm | |||||||||||||||||||||||||||
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検出器 | 名称: Pilatus 1M | |||||||||||||||||||||||||||
スキャン | タイトル: Dark state: Aureochrome1a- A´α-LOV-Jα / 測定日: 2015年3月16日 / 保管温度: 4 °C / 照射時間: 20 sec. / フレーム数: 10 / 単位: 1/nm /
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距離分布関数 P(R) | ソフトウェア P(R): GNOM 5.0 / ポイント数: 408 /
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結果 | カーブのタイプ: single_conc コメント: Solution scattering of N-terminally His6 tagged A´α-LOV-Jα was measured in the dark state (under safe red light). Two concentrations of the sample were measured at 40 and 833 ...コメント: Solution scattering of N-terminally His6 tagged A´α-LOV-Jα was measured in the dark state (under safe red light). Two concentrations of the sample were measured at 40 and 833 micromolar and then merged. Radiation damage was observed at very low extent. The data were normalized and the buffer only scattering subtracted. The analysis was performed using ATSAS. The displayed model is an averaged and volume corrected dummy atom representation (DAMFILT). For further sequence information refer to the Joint Genome Institute (JGI) entry JGI-49116.
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