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Open data
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Basic information
Entry | Database: PDB / ID: 7nho | ||||||||||||
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Title | Structure of PSII-M | ||||||||||||
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Function / homology | ![]() oxygen evolving activity / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||||||||
Biological species | ![]() ![]() | ||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||
![]() | Zabret, J. / Bohn, S. / Schuller, S.K. / Arnolds, O. / Chan, A. / Tajkhorshid, E. / Stoll, R. / Engel, B.D. / Rudack, T. / Schuller, J.M. / Nowaczyk, M.M. | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural insights into photosystem II assembly. Authors: Jure Zabret / Stefan Bohn / Sandra K Schuller / Oliver Arnolds / Madeline Möller / Jakob Meier-Credo / Pasqual Liauw / Aaron Chan / Emad Tajkhorshid / Julian D Langer / Raphael Stoll / Anja ...Authors: Jure Zabret / Stefan Bohn / Sandra K Schuller / Oliver Arnolds / Madeline Möller / Jakob Meier-Credo / Pasqual Liauw / Aaron Chan / Emad Tajkhorshid / Julian D Langer / Raphael Stoll / Anja Krieger-Liszkay / Benjamin D Engel / Till Rudack / Jan M Schuller / Marc M Nowaczyk / ![]() ![]() ![]() Abstract: Biogenesis of photosystem II (PSII), nature's water-splitting catalyst, is assisted by auxiliary proteins that form transient complexes with PSII components to facilitate stepwise assembly events. ...Biogenesis of photosystem II (PSII), nature's water-splitting catalyst, is assisted by auxiliary proteins that form transient complexes with PSII components to facilitate stepwise assembly events. Using cryo-electron microscopy, we solved the structure of such a PSII assembly intermediate from Thermosynechococcus elongatus at 2.94 Å resolution. It contains three assembly factors (Psb27, Psb28 and Psb34) and provides detailed insights into their molecular function. Binding of Psb28 induces large conformational changes at the PSII acceptor side, which distort the binding pocket of the mobile quinone (Q) and replace the bicarbonate ligand of non-haem iron with glutamate, a structural motif found in reaction centres of non-oxygenic photosynthetic bacteria. These results reveal mechanisms that protect PSII from damage during biogenesis until water splitting is activated. Our structure further demonstrates how the PSII active site is prepared for the incorporation of the MnCaO cluster, which performs the unique water-splitting reaction. | ||||||||||||
History |
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Structure visualization
Movie |
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Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 445.9 KB | Display | ![]() |
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PDB format | ![]() | 375.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 12335MC ![]() 7nhpC ![]() 7nhqC M: map data used to model this data C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Components
-Photosystem II ... , 13 types, 13 molecules ABCDHIKLMTXyZ
#1: Protein | ![]() Mass: 39762.309 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) ![]() ![]() References: UniProt: P0A444, ![]() |
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#2: Protein | ![]() Mass: 56656.457 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) ![]() ![]() References: UniProt: Q8DIQ1 |
#3: Protein | ![]() Mass: 50287.500 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) ![]() ![]() References: UniProt: Q8DIF8 |
#4: Protein | ![]() Mass: 39388.156 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) ![]() ![]() References: UniProt: Q8CM25, ![]() |
#7: Protein | ![]() Mass: 7358.754 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) ![]() ![]() References: UniProt: Q8DJ43 |
#8: Protein/peptide | ![]() Mass: 4410.245 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) ![]() ![]() References: UniProt: Q8DJZ6 |
#9: Protein/peptide | ![]() Mass: 5028.083 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) ![]() ![]() References: UniProt: Q9F1K9 |
#10: Protein/peptide | ![]() Mass: 4299.044 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) ![]() ![]() References: UniProt: Q8DIN8 |
#11: Protein/peptide | ![]() Mass: 3981.673 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) ![]() ![]() References: UniProt: Q8DHA7 |
#12: Protein/peptide | ![]() Mass: 3878.728 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) ![]() ![]() References: UniProt: Q8DIQ0 |
#13: Protein/peptide | ![]() Mass: 4322.226 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) ![]() ![]() References: UniProt: Q9F1R6 |
#14: Protein/peptide | ![]() Mass: 5039.143 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) ![]() ![]() References: UniProt: Q8DJI1 |
#15: Protein | ![]() Mass: 6766.187 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) ![]() ![]() References: UniProt: Q8DHJ2 |
-Cytochrome b559 subunit ... , 2 types, 2 molecules EF
#5: Protein | ![]() Mass: 9580.840 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) ![]() ![]() References: UniProt: Q8DIP0 |
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#6: Protein/peptide | ![]() Mass: 5067.900 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) ![]() ![]() References: UniProt: Q8DIN9 |
-Non-polymers , 11 types, 62 molecules ![](data/chem/img/FE.gif)
![](data/chem/img/MN.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/PHO.gif)
![](data/chem/img/CLA.gif)
![](data/chem/img/BCR.gif)
![](data/chem/img/LHG.gif)
![](data/chem/img/BCT.gif)
![](data/chem/img/LMG.gif)
![](data/chem/img/PL9.gif)
![](data/chem/img/HEM.gif)
![](data/chem/img/MN.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/PHO.gif)
![](data/chem/img/CLA.gif)
![](data/chem/img/BCR.gif)
![](data/chem/img/LHG.gif)
![](data/chem/img/BCT.gif)
![](data/chem/img/LMG.gif)
![](data/chem/img/PL9.gif)
![](data/chem/img/HEM.gif)
#16: Chemical | ChemComp-FE / ![]() | ||||||||||||||
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#17: Chemical | ChemComp-MN / | ||||||||||||||
#18: Chemical | ChemComp-CL / ![]() | ||||||||||||||
#19: Chemical | ![]() #20: Chemical | ChemComp-CLA / ![]() #21: Chemical | ChemComp-BCR / ![]() #22: Chemical | ![]() #23: Chemical | ChemComp-BCT / | ![]() #24: Chemical | ChemComp-LMG / #25: Chemical | ChemComp-PL9 / | ![]() #26: Chemical | ChemComp-HEM / | ![]() |
-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: ![]() |
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Sample preparation
Component | Name: PSII-M / Type: COMPLEX / Entity ID: #1-#15 / Source: NATURAL |
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Molecular weight | Experimental value: NO |
Source (natural) | Organism: ![]() ![]() |
Buffer solution | pH: 6.5 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied![]() ![]() |
Vitrification![]() | Cryogen name: ETHANE-PROPANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source![]() ![]() |
Electron lens | Mode: BRIGHT FIELD![]() |
Image recording | Electron dose: 55 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
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Processing
CTF correction![]() | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
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3D reconstruction![]() | Resolution: 2.66 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 166411 / Symmetry type: POINT |