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- PDB-7cgp: Cryo-EM structure of the human mitochondrial translocase TIM22 co... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7cgp | |||||||||
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Title | Cryo-EM structure of the human mitochondrial translocase TIM22 complex at 3.7 angstrom. | |||||||||
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![]() | TRANSLOCASE / human mitochondrial translocase TIM22 complex / inner membrane / chaperone Tim9/10a / chaperone Tim9/10a/10b | |||||||||
Function / homology | ![]() ceramide kinase / acylglycerol kinase / ceramide kinase activity / acylglycerol kinase activity / dihydroceramide kinase activity / Glycerophospholipid biosynthesis / mitochondrial intermembrane space protein transporter complex / glycerolipid metabolic process / : / lipid kinase activity ...ceramide kinase / acylglycerol kinase / ceramide kinase activity / acylglycerol kinase activity / dihydroceramide kinase activity / Glycerophospholipid biosynthesis / mitochondrial intermembrane space protein transporter complex / glycerolipid metabolic process / : / lipid kinase activity / diacylglycerol kinase (ATP) / ATP-dependent diacylglycerol kinase activity / membrane insertase activity / TIM23 mitochondrial import inner membrane translocase complex / mitochondrion targeting sequence binding / TIM22 mitochondrial import inner membrane insertion complex / NAD+ kinase activity / protein transporter activity / protein insertion into mitochondrial inner membrane / sphingolipid metabolic process / ceramide biosynthetic process / Mitochondrial protein import / protein targeting to mitochondrion / protein transmembrane transporter activity / Mitochondrial protein degradation / cell-matrix adhesion / mitochondrial membrane / sensory perception of sound / mitochondrial intermembrane space / Signaling by BRAF and RAF1 fusions / protein-folding chaperone binding / mitochondrial outer membrane / mitochondrial inner membrane / membrane => GO:0016020 / phosphorylation / intracellular membrane-bounded organelle / protein homodimerization activity / mitochondrion / zinc ion binding / ATP binding / membrane / metal ion binding / cytoplasm / cytosol Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.7 Å | |||||||||
![]() | Qi, L. / Wang, Q. / Guan, Z. / Yan, C. / Yin, P. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Cryo-EM structure of the human mitochondrial translocase TIM22 complex. Authors: Liangbo Qi / Qiang Wang / Zeyuan Guan / Yan Wu / Cuicui Shen / Sixing Hong / Jianbo Cao / Xing Zhang / Chuangye Yan / Ping Yin / ![]() | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 281.4 KB | Display | ![]() |
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PDB format | ![]() | 233.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 949.3 KB | Display | ![]() |
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Full document | ![]() | 972.1 KB | Display | |
Data in XML | ![]() | 48.3 KB | Display | |
Data in CIF | ![]() | 72.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 9958MC M: map data used to model this data C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Components
-Mitochondrial import inner membrane translocase subunit ... , 5 types, 14 molecules ACDEFKLGHIMNOJ
#1: Protein | Mass: 20048.930 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() | ||||
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#3: Protein | Mass: 29272.336 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() | ||||
#4: Protein | Mass: 10391.906 Da / Num. of mol.: 5 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #5: Protein | Mass: 10348.999 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #6: Protein | | Mass: 11601.244 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
-Protein / Non-polymers , 2 types, 2 molecules B![](data/chem/img/PEE.gif)
![](data/chem/img/PEE.gif)
#2: Protein | Mass: 47196.008 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() References: UniProt: Q53H12, diacylglycerol kinase (ATP), ceramide kinase, acylglycerol kinase |
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#7: Chemical | ChemComp-PEE / |
-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: TIM22 translocase / Type: COMPLEX / Entity ID: #1-#2, #4-#5 / Source: RECOMBINANT |
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Molecular weight | Value: 220 kDa/nm / Experimental value: NO |
Source (natural) | Organism: ![]() |
Source (recombinant) | Organism: ![]() |
Buffer solution | pH: 6 |
Specimen | Conc.: 7 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD |
Image recording | Electron dose: 60 e/Å2 / Detector mode: SUPER-RESOLUTION / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
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Processing
Software | Name: PHENIX / Version: 1.18.2_3874: / Classification: refinement | ||||||||||||||||||||||||
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EM software |
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CTF correction | Type: NONE | ||||||||||||||||||||||||
Symmetry | Point symmetry: C1 (asymmetric) | ||||||||||||||||||||||||
3D reconstruction | Resolution: 3.7 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 482959 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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