+
Open data
-
Basic information
Entry | Database: PDB / ID: 7boy | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Mature bacteriorphage t7 tail nozzle protein gp12 | |||||||||
![]() | Tail tubular protein gp12 | |||||||||
![]() | STRUCTURAL PROTEIN / phage / portal / mature / protein | |||||||||
Function / homology | virus tail, tube / symbiont genome ejection through host cell envelope, short tail mechanism / Tail tubular protein gp12![]() | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.8 Å | |||||||||
![]() | Chen, W.Y. / Xiao, H. | |||||||||
Funding support | 2items
| |||||||||
![]() | ![]() Title: Structural changes of a bacteriophage upon DNA packaging and maturation. Authors: Wenyuan Chen / Hao Xiao / Xurong Wang / Shuanglin Song / Zhen Han / Xiaowu Li / Fan Yang / Li Wang / Jingdong Song / Hongrong Liu / Lingpeng Cheng / ![]() | |||||||||
History |
|
-
Structure visualization
Movie |
![]() |
---|---|
Structure viewer | Molecule: ![]() ![]() |
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 803.9 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 676.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 722.7 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 753.8 KB | Display | |
Data in XML | ![]() | 115.2 KB | Display | |
Data in CIF | ![]() | 177.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 30136MC ![]() 7bouC ![]() 7boxC ![]() 7bozC ![]() 7bp0C M: map data used to model this data C: citing same article ( |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
|
---|---|
1 |
|
-
Components
#1: Protein | Mass: 89480.594 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
---|
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
---|---|
EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-
Sample preparation
Component | Name: Mature bacteriorphage t7 tail nozzle protein gp12 / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
---|---|
Source (natural) | Organism: ![]() ![]() |
Buffer solution | pH: 7 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: NITROGEN |
-
Electron microscopy imaging
Experimental equipment | ![]() Model: Talos Arctica / Image courtesy: FEI Company |
---|---|
Microscopy | Model: FEI TECNAI ARCTICA |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD |
Image recording | Electron dose: 24 e/Å2 / Film or detector model: FEI FALCON II (4k x 4k) |
-
Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
---|---|
3D reconstruction | Resolution: 3.8 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 55212 / Symmetry type: POINT |