National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R37 GM057247
米国
引用
ジャーナル: Proc Natl Acad Sci U S A / 年: 2018 タイトル: High-resolution cryo-EM structures of actin-bound myosin states reveal the mechanism of myosin force sensing. 著者: Ahmet Mentes / Andrew Huehn / Xueqi Liu / Adam Zwolak / Roberto Dominguez / Henry Shuman / E Michael Ostap / Charles V Sindelar / 要旨: Myosins adjust their power outputs in response to mechanical loads in an isoform-dependent manner, resulting in their ability to dynamically adapt to a range of motile challenges. Here, we reveal the ...Myosins adjust their power outputs in response to mechanical loads in an isoform-dependent manner, resulting in their ability to dynamically adapt to a range of motile challenges. Here, we reveal the structural basis for force-sensing based on near-atomic resolution structures of one rigor and two ADP-bound states of myosin-IB (myo1b) bound to actin, determined by cryo-electron microscopy. The two ADP-bound states are separated by a 25° rotation of the lever. The lever of the first ADP state is rotated toward the pointed end of the actin filament and forms a previously unidentified interface with the N-terminal subdomain, which constitutes the upper half of the nucleotide-binding cleft. This pointed-end orientation of the lever blocks ADP release by preventing the N-terminal subdomain from the pivoting required to open the nucleotide binding site, thus revealing how myo1b is inhibited by mechanical loads that restrain lever rotation. The lever of the second ADP state adopts a rigor-like orientation, stabilized by class-specific elements of myo1b. We identify a role for this conformation as an intermediate in the ADP release pathway. Moreover, comparison of our structures with other myosins reveals structural diversity in the actomyosin binding site, and we reveal the high-resolution structure of actin-bound phalloidin, a potent stabilizer of filamentous actin. These results provide a framework to understand the spectrum of force-sensing capacities among the myosin superfamily.
平均露光時間: 11 sec. / 電子線照射量: 50 e/Å2 フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k)
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解析
ソフトウェア
名称: PHENIX / バージョン: 1.11.1_2575: / 分類: 精密化
CTF補正
タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION
らせん対称
回転角度/サブユニット: -167.4 ° / 軸方向距離/サブユニット: 27.5 Å / らせん対称軸の対称性: C1
3次元再構成
解像度: 3.9 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 62000 詳細: Resolution estimated by post-processing in RELION using a mask with soft edges that included only the central subunit. 対称性のタイプ: HELICAL