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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-32527 | |||||||||
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Title | Cryo-EM structure of inactive mGlu3 bound to LY341495 | |||||||||
![]() | Cryo-EM structure of inactive mGlu3 bound to LY341495 | |||||||||
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Function / homology | ![]() group II metabotropic glutamate receptor activity / negative regulation of adenylate cyclase activity / G protein-coupled glutamate receptor signaling pathway / astrocyte projection / Class C/3 (Metabotropic glutamate/pheromone receptors) / glutamate receptor activity / postsynaptic modulation of chemical synaptic transmission / calcium channel regulator activity / regulation of synaptic transmission, glutamatergic / G protein-coupled receptor activity ...group II metabotropic glutamate receptor activity / negative regulation of adenylate cyclase activity / G protein-coupled glutamate receptor signaling pathway / astrocyte projection / Class C/3 (Metabotropic glutamate/pheromone receptors) / glutamate receptor activity / postsynaptic modulation of chemical synaptic transmission / calcium channel regulator activity / regulation of synaptic transmission, glutamatergic / G protein-coupled receptor activity / presynaptic membrane / gene expression / G alpha (i) signalling events / scaffold protein binding / chemical synaptic transmission / postsynaptic membrane / dendritic spine / postsynaptic density / axon / glutamatergic synapse / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.17 Å | |||||||||
![]() | Fang W / Yang F / Xu CJ / Ling SL / Lin L / Zhou YX / Sun WJ / Wang XM / Liu P / Rondard P ...Fang W / Yang F / Xu CJ / Ling SL / Lin L / Zhou YX / Sun WJ / Wang XM / Liu P / Rondard P / Pan S / Pin JP / Tian CL / Liu JF | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural basis of the activation of metabotropic glutamate receptor 3. Authors: Wei Fang / Fan Yang / Chanjuan Xu / Shenglong Ling / Li Lin / Yingxin Zhou / Wenjing Sun / Xiaomei Wang / Peng Liu / Philippe Rondard / Pan Shi / Jean-Philippe Pin / Changlin Tian / Jianfeng Liu / ![]() ![]() | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 117.7 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 12.2 KB 12.2 KB | Display Display | ![]() |
Images | ![]() | 67.5 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 417.5 KB | Display | ![]() |
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Full document | ![]() | 417.1 KB | Display | |
Data in XML | ![]() | 6.5 KB | Display | |
Data in CIF | ![]() | 7.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7wi8MC ![]() 7wi6C ![]() 7wihC C: citing same article ( M: atomic model generated by this map |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Annotation | Cryo-EM structure of inactive mGlu3 bound to LY341495 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.01 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : Cryo-EM structure of mGlu3 bound with LY2794193
Entire | Name: Cryo-EM structure of mGlu3 bound with LY2794193 |
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Components |
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-Supramolecule #1: Cryo-EM structure of mGlu3 bound with LY2794193
Supramolecule | Name: Cryo-EM structure of mGlu3 bound with LY2794193 / type: complex / Chimera: Yes / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() |
Recombinant expression | Organism: ![]() ![]() |
-Macromolecule #1: Metabotropic glutamate receptor 3
Macromolecule | Name: Metabotropic glutamate receptor 3 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 100.021695 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MKTIIALSYI FCLVFADYKD DDDENLYFQG LGDHNFLRRE IKIEGDLVLG GLFPINEKGT GTEECGRINE DRGIQRLEAM LFAIDEINK DDYLLPGVKL GVHILDTCSR DTYALEQSLE FVRASLTKVD EAEYMCPDGS YAIQENIPLL IAGVIGGSYS S VSIQVANL ...String: MKTIIALSYI FCLVFADYKD DDDENLYFQG LGDHNFLRRE IKIEGDLVLG GLFPINEKGT GTEECGRINE DRGIQRLEAM LFAIDEINK DDYLLPGVKL GVHILDTCSR DTYALEQSLE FVRASLTKVD EAEYMCPDGS YAIQENIPLL IAGVIGGSYS S VSIQVANL LRLFQIPQIS YASTSAKLSD KSRYDYFART VPPDFYQAKA MAEILRFFNW TYVSTVASEG DYGETGIEAF EQ EARLRNI CIATAEKVGR SNIRKSYDSV IRELLQKPNA RVVVLFMRSD DSRELIAAAS RANASFTWVA SDGWGAQESI IKG SEHVAY GAITLELASQ PVRQFDRYFQ SLNPYNNHRN PWFRDFWEQK FQCSLQNKRN HRRVCDKHLA IDSSNYEQES KIMF VVNAV YAMAHALHKM QRTLCPNTTK LCDAMKILDG KKLYKDYLLK INFTAPFNPN KDADSIVKFD TFGDGMGRYN VFNFQ NVGG KYSYLKVGHW AETLSLDVNS IHWSRNSVPT SQCSDPCAPN EMKNMQPGDV CCWICIPCEP YEYLADEFTC MDCGSG QWP TADLTGCYDL PEDYIRWEDA WAIGPVTIAC LGFMCTCMVV TVFIKHNNTP LVKASGRELC YILLFGVGLS YCMTFFF IA KPSPVICALR RLGLGSSFAI CYSALLTKTN CIARIFDGVK NGAQRPKFIS PSSQVFICLG LILVQIVMVS VWLILEAP G TRRYTLAEKR ETVILKCNVK DSSMLISLTY DVILVILCTV YAFKTRKCPE NFNEAKFIGF TMYTTCIIWL AFLPIFYVT SSDYRVQTTT MCISVSLSGF VVLGCLFAPK VHIILFQPQK NVVTHRLHLN RFSVSGTGTT YSQSSASTYV PTVCNGREVL DSTTSSL |
-Macromolecule #2: 2-acetamido-2-deoxy-beta-D-glucopyranose
Macromolecule | Name: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 2 / Number of copies: 2 / Formula: NAG |
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Molecular weight | Theoretical: 221.208 Da |
Chemical component information | ![]() ChemComp-NAG: |
-Macromolecule #3: 2-[(1S,2S)-2-carboxycyclopropyl]-3-(9H-xanthen-9-yl)-D-alanine
Macromolecule | Name: 2-[(1S,2S)-2-carboxycyclopropyl]-3-(9H-xanthen-9-yl)-D-alanine type: ligand / ID: 3 / Number of copies: 2 / Formula: Z99 |
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Molecular weight | Theoretical: 353.369 Da |
Chemical component information | ![]() ChemComp-Z99: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 10 mg/mL |
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Buffer | pH: 7.5 |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 57.6 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.8 µm / Nominal defocus min: 1.5 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |