ジャーナル: Cell Rep / 年: 2014 タイトル: Cryoelectron microscopic structures of eukaryotic translation termination complexes containing eRF1-eRF3 or eRF1-ABCE1. 著者: Anne Preis / Andre Heuer / Clara Barrio-Garcia / Andreas Hauser / Daniel E Eyler / Otto Berninghausen / Rachel Green / Thomas Becker / Roland Beckmann / 要旨: Termination and ribosome recycling are essential processes in translation. In eukaryotes, a stop codon in the ribosomal A site is decoded by a ternary complex consisting of release factors eRF1 and ...Termination and ribosome recycling are essential processes in translation. In eukaryotes, a stop codon in the ribosomal A site is decoded by a ternary complex consisting of release factors eRF1 and guanosine triphosphate (GTP)-bound eRF3. After GTP hydrolysis, eRF3 dissociates, and ABCE1 can bind to eRF1-loaded ribosomes to stimulate peptide release and ribosomal subunit dissociation. Here, we present cryoelectron microscopic (cryo-EM) structures of a pretermination complex containing eRF1-eRF3 and a termination/prerecycling complex containing eRF1-ABCE1. eRF1 undergoes drastic conformational changes: its central domain harboring the catalytically important GGQ loop is either packed against eRF3 or swung toward the peptidyl transferase center when bound to ABCE1. Additionally, in complex with eRF3, the N-terminal domain of eRF1 positions the conserved NIKS motif proximal to the stop codon, supporting its suggested role in decoding, yet it appears to be delocalized in the presence of ABCE1. These results suggest that stop codon decoding and peptide release can be uncoupled during termination.
#241 - 2020年1月 20年の分子を振り返って (Twenty Years of Molecules) 類似性 (4)
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マップ
ファイル
ダウンロード / ファイル: emd_2598.map.gz / 形式: CCP4 / 大きさ: 185.7 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
"CMV-stalled" wheat germ 80S-RNC bound to eRF1 and ABCE1.
ボクセルのサイズ
X=Y=Z: 1.2375 Å
密度
表面レベル
登録者による: 0.232 / ムービー #1: 0.232
最小 - 最大
-0.63350767 - 1.32882106
平均 (標準偏差)
0.00894418 (±0.0960145)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
Y
X
Z
Origin
-184
-184
-183
サイズ
368
368
368
Spacing
368
368
368
セル
A=B=C: 455.4 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
1.2375
1.2375
1.2375
M x/y/z
368
368
368
origin x/y/z
0.000
0.000
0.000
length x/y/z
455.400
455.400
455.400
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
-184
-184
-183
NX/NY/NZ
368
368
368
MAP C/R/S
2
1
3
start NC/NR/NS
-184
-184
-183
NC/NR/NS
368
368
368
D min/max/mean
-0.634
1.329
0.009
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添付データ
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試料の構成要素
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全体 : "CMV"-stalled wheat germ 80S-RNC bound to eRF1 and ABCE1-ADPNP
全体
名称: "CMV"-stalled wheat germ 80S-RNC bound to eRF1 and ABCE1-ADPNP
要素
試料: "CMV"-stalled wheat germ 80S-RNC bound to eRF1 and ABCE1-ADPNP
複合体: 80S ribosome
タンパク質・ペプチド: Sup45
タンパク質・ペプチド: Rli1
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超分子 #1000: "CMV"-stalled wheat germ 80S-RNC bound to eRF1 and ABCE1-ADPNP
超分子
名称: "CMV"-stalled wheat germ 80S-RNC bound to eRF1 and ABCE1-ADPNP タイプ: sample / ID: 1000 / 集合状態: One ribosome binds to one eRF1 and one ABCE1 / Number unique components: 3
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超分子 #1: 80S ribosome
超分子
名称: 80S ribosome / タイプ: complex / ID: 1 / 組換発現: No / Ribosome-details: ribosome-eukaryote: ALL