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TitleCryoelectron microscopic structures of eukaryotic translation termination complexes containing eRF1-eRF3 or eRF1-ABCE1.
Journal, issue, pagesCell Rep, Vol. 8, Issue 1, Page 59-65, Year 2014
Publish dateJul 10, 2014
AuthorsAnne Preis / Andre Heuer / Clara Barrio-Garcia / Andreas Hauser / Daniel E Eyler / Otto Berninghausen / Rachel Green / Thomas Becker / Roland Beckmann /
PubMed AbstractTermination and ribosome recycling are essential processes in translation. In eukaryotes, a stop codon in the ribosomal A site is decoded by a ternary complex consisting of release factors eRF1 and ...Termination and ribosome recycling are essential processes in translation. In eukaryotes, a stop codon in the ribosomal A site is decoded by a ternary complex consisting of release factors eRF1 and guanosine triphosphate (GTP)-bound eRF3. After GTP hydrolysis, eRF3 dissociates, and ABCE1 can bind to eRF1-loaded ribosomes to stimulate peptide release and ribosomal subunit dissociation. Here, we present cryoelectron microscopic (cryo-EM) structures of a pretermination complex containing eRF1-eRF3 and a termination/prerecycling complex containing eRF1-ABCE1. eRF1 undergoes drastic conformational changes: its central domain harboring the catalytically important GGQ loop is either packed against eRF3 or swung toward the peptidyl transferase center when bound to ABCE1. Additionally, in complex with eRF3, the N-terminal domain of eRF1 positions the conserved NIKS motif proximal to the stop codon, supporting its suggested role in decoding, yet it appears to be delocalized in the presence of ABCE1. These results suggest that stop codon decoding and peptide release can be uncoupled during termination.
External linksCell Rep / PubMed:25001285 / PubMed Central
MethodsEM (single particle)
Resolution8.75 - 9.15 Å
Structure data

2597

4crn

EMDB-2597, PDB-4crn:
Cryo-EM of a pretermination complex with eRF1 and eRF3
Method: EM (single particle) / Resolution: 9.15 Å

2598

4crm

EMDB-2598: Cryo-EM of a termination/pre-recycling complex with eRF1 and ABCE1
PDB-4crm: Cryo-EM of a pre-recycling complex with eRF1 and ABCE1
Method: EM (single particle) / Resolution: 8.75 Å

Chemicals

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

ChemComp-SF4:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER / Water

ChemComp-GNP:
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / GppNHp, GMPPNP, energy-carrying molecule analogue*YM / 5'-Guanylyl imidodiphosphate

Source
  • Triticum aestivum (bread wheat)
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsTRANSLATION / TERMINATION / RECYCLING / CRYO-EM

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