登録情報 データベース : EMDB / ID : EMD-20032 構造の表示 ダウンロードとリンクタイトル Cryo-EM structure of mouse RAG1/2 NFC complex (DNA1) マップデータstructure of mouse RAG1/2 NFC complex (DNA1) 詳細 試料複合体 : RAG1/2 Nink-forming complex (DNA1)タンパク質・ペプチド : V(D)J recombination-activating protein 1タンパク質・ペプチド : V(D)J recombination-activating protein 2DNA : DNA (46-MER)DNA : DNA (46-MER)DNA : DNA (57-MER)DNA : DNA (57-MER)タンパク質・ペプチド : High mobility group protein B1 高移動度群タンパク質 残り3件を表示 表示を減らすリガンド : CALCIUM ION カルシウムリガンド : ZINC ION 詳細 キーワード V(D)J recombination (V(D)J遺伝子再構成) / DNA Transposition / RAG / SCID / RECOMBINATION (遺伝的組換え) / RECOMBINATION-DNA complex機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
regulation of restriction endodeoxyribonuclease activity / regulation of tolerance induction / regulation of T cell mediated immune response to tumor cell / positive regulation of mismatch repair / negative regulation of apoptotic cell clearance / negative regulation of RNA polymerase II transcription preinitiation complex assembly / DNA geometric change / myeloid dendritic cell activation / mature B cell differentiation involved in immune response / T-helper 1 cell activation ... regulation of restriction endodeoxyribonuclease activity / regulation of tolerance induction / regulation of T cell mediated immune response to tumor cell / positive regulation of mismatch repair / negative regulation of apoptotic cell clearance / negative regulation of RNA polymerase II transcription preinitiation complex assembly / DNA geometric change / myeloid dendritic cell activation / mature B cell differentiation involved in immune response / T-helper 1 cell activation / C-X-C chemokine binding / T-helper 1 cell differentiation / positive regulation of dendritic cell differentiation / DNA recombinase complex / endodeoxyribonuclease complex / negative regulation of CD4-positive, alpha-beta T cell differentiation / B cell homeostatic proliferation / DN2 thymocyte differentiation / negative regulation of T cell differentiation in thymus / positive regulation of toll-like receptor 9 signaling pathway / neutrophil clearance / positive regulation of DNA ligation / pre-B cell allelic exclusion / positive regulation of organ growth / positive regulation of interleukin-1 production / Regulation of TLR by endogenous ligand / RAGE receptor binding / regulation of behavioral fear response / alphav-beta3 integrin-HMGB1 complex / bubble DNA binding / V(D)J遺伝子再構成 / negative regulation of T cell apoptotic process / Apoptosis induced DNA fragmentation / phosphatidylinositol-3,4-bisphosphate binding / inflammatory response to antigenic stimulus / negative regulation of thymocyte apoptotic process / supercoiled DNA binding / positive regulation of chemokine (C-X-C motif) ligand 2 production / MyD88 deficiency (TLR2/4) / positive regulation of monocyte chemotaxis / phosphatidylinositol-3,5-bisphosphate binding / apoptotic cell clearance / dendritic cell chemotaxis / positive regulation of T cell differentiation / IRAK4 deficiency (TLR2/4) / DNA binding, bending / positive regulation of vascular endothelial cell proliferation / regulation of T cell differentiation / MyD88:MAL(TIRAP) cascade initiated on plasma membrane / organ growth / T cell lineage commitment / B cell lineage commitment / positive regulation of activated T cell proliferation / phosphatidylserine binding / chemoattractant activity / T cell homeostasis / phosphatidylinositol-3,4,5-trisphosphate binding / DNA topological change / positive regulation of interleukin-10 production / TRAF6 mediated NF-kB activation / positive regulation of cysteine-type endopeptidase activity involved in apoptotic process / Advanced glycosylation endproduct receptor signaling / negative regulation of blood vessel endothelial cell migration / negative regulation of type II interferon production / T cell differentiation / endoplasmic reticulum-Golgi intermediate compartment / positive regulation of blood vessel endothelial cell migration / positive regulation of DNA binding / protein autoubiquitination / Pyroptosis / positive regulation of autophagy / heterochromatin formation / DNA polymerase binding / four-way junction DNA binding / condensed chromosome / methylated histone binding / phosphatidylinositol-4,5-bisphosphate binding / transcription repressor complex / activation of innate immune response / positive regulation of interleukin-12 production / phosphatidylinositol binding / B cell differentiation / thymus development / cytokine activity / positive regulation of interleukin-8 production / lipopolysaccharide binding / positive regulation of JNK cascade / TAK1-dependent IKK and NF-kappa-B activation / RING-type E3 ubiquitin transferase / visual learning / オートファジー / double-strand break repair via nonhomologous end joining / ubiquitin-protein transferase activity / positive regulation of interleukin-6 production / transcription corepressor activity / neuron projection development / positive regulation of tumor necrosis factor production / ubiquitin protein ligase activity / integrin binding / chromatin organization 類似検索 - 分子機能 HMG box A DNA-binding domain, conserved site / Recombination-activating protein 1 zinc-finger domain / HMG box A DNA-binding domain signature. / V(D)J recombination-activating protein 1, Zinc finger / RAG nonamer-binding domain / NBD domain profile. / Zinc finger RAG1-type profile. / V(D)J recombination-activating protein 1 / RAG1 importin-binding / RAG1 importin binding ... HMG box A DNA-binding domain, conserved site / Recombination-activating protein 1 zinc-finger domain / HMG box A DNA-binding domain signature. / V(D)J recombination-activating protein 1, Zinc finger / RAG nonamer-binding domain / NBD domain profile. / Zinc finger RAG1-type profile. / V(D)J recombination-activating protein 1 / RAG1 importin-binding / RAG1 importin binding / Recombination-activation protein 1 (RAG1), recombinase / Recombination activating protein 2 / RAG2 PHD domain / V-D-J recombination activating protein 2 / Recombination activating protein 2, PHD domain / Galactose oxidase/kelch, beta-propeller / HMG-box domain / HMG (high mobility group) box / Zinc finger, C3HC4 RING-type / Zinc finger, C3HC4 type (RING finger) / Kelch-type beta propeller / HMG boxes A and B DNA-binding domains profile. / 高移動度群タンパク質 / High mobility group box domain / High mobility group box domain superfamily / Zinc finger, RING-type, conserved site / Zinc finger RING-type signature. / Zinc finger C2H2 superfamily / 薬指 / Zinc finger RING-type profile. / Zinc finger, RING-type / Zinc finger, FYVE/PHD-type / Zinc finger, RING/FYVE/PHD-type 類似検索 - ドメイン・相同性 High mobility group protein B1 / V(D)J recombination-activating protein 1 / V(D)J recombination-activating protein 2 類似検索 - 構成要素生物種 Mus musculus (ハツカネズミ) / Homo sapiens (ヒト) / Escherichia coli K-12 (大腸菌)手法 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 3.69 Å 詳細 データ登録者Chen X / Cui Y / Zhou ZH / Yang W / Gellert M 資金援助 米国, 1件 詳細 詳細を隠すOrganization Grant number 国 National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK) DK036167 米国
引用ジャーナル : Nat Struct Mol Biol / 年 : 2020タイトル : Cutting antiparallel DNA strands in a single active site.著者 : Xuemin Chen / Yanxiang Cui / Robert B Best / Huaibin Wang / Z Hong Zhou / Wei Yang / Martin Gellert / 要旨 : A single enzyme active site that catalyzes multiple reactions is a well-established biochemical theme, but how one nuclease site cleaves both DNA strands of a double helix has not been well ... A single enzyme active site that catalyzes multiple reactions is a well-established biochemical theme, but how one nuclease site cleaves both DNA strands of a double helix has not been well understood. In analyzing site-specific DNA cleavage by the mammalian RAG1-RAG2 recombinase, which initiates V(D)J recombination, we find that the active site is reconfigured for the two consecutive reactions and the DNA double helix adopts drastically different structures. For initial nicking of the DNA, a locally unwound and unpaired DNA duplex forms a zipper via alternating interstrand base stacking, rather than melting as generally thought. The second strand cleavage and formation of a hairpin-DNA product requires a global scissor-like movement of protein and DNA, delivering the scissile phosphate into the rearranged active site. 履歴 登録 2019年3月27日 - ヘッダ(付随情報) 公開 2019年5月15日 - マップ公開 2020年1月29日 - 更新 2024年3月20日 - 現状 2024年3月20日 処理サイト : RCSB / 状態 : 公開
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