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- EMDB-7851: Cryo-EM structure of RAG in complex with melted 12-RSS and unmelt... -

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Basic information

Entry
Database: EMDB / ID: EMD-7851
TitleCryo-EM structure of RAG in complex with melted 12-RSS and unmelted 23-RSS substrates
Map dataRAG in complex with 12-RSS and 23-RSS substrate DNAs
Sample
  • Complex: RAG in complex with 12-RSS and 23-RSS substrate DNAs
    • Protein or peptide: Recombination activating gene 1 - MBP chimera
    • Protein or peptide: Recombination activating gene 2
    • Protein or peptide: Recombination activating gene 1 - MBP chimera
    • DNA: Forward strand of 12-RSS substrate DNA
    • DNA: Reverse strand of 12-RSS substrate DNA
    • DNA: Forward strand of 23-RSS substrate DNA
    • DNA: Reverse strand of 23-RSS substrate DNA
  • Ligand: ZINC ION
  • Ligand: CALCIUM ION
KeywordsV(D)J recombination / RAG complex / Melted RSS / Unmelted RSS / Recombination-DNA complex
Function / homology
Function and homology information


somatic diversification of immune receptors via germline recombination within a single locus / hematopoietic or lymphoid organ development / DNA recombinase complex / endodeoxyribonuclease complex / protein-DNA complex assembly / lymphocyte differentiation / immunoglobulin V(D)J recombination / V(D)J recombination / phosphatidylinositol-3,4-bisphosphate binding / histone H3K4me3 reader activity ...somatic diversification of immune receptors via germline recombination within a single locus / hematopoietic or lymphoid organ development / DNA recombinase complex / endodeoxyribonuclease complex / protein-DNA complex assembly / lymphocyte differentiation / immunoglobulin V(D)J recombination / V(D)J recombination / phosphatidylinositol-3,4-bisphosphate binding / histone H3K4me3 reader activity / phosphatidylinositol-3,5-bisphosphate binding / detection of maltose stimulus / maltose transport complex / phosphatidylinositol-3,4,5-trisphosphate binding / carbohydrate transport / T cell differentiation / carbohydrate transmembrane transporter activity / maltose binding / maltose transport / maltodextrin transmembrane transport / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / phosphatidylinositol-4,5-bisphosphate binding / phosphatidylinositol binding / ATP-binding cassette (ABC) transporter complex / thymus development / B cell differentiation / cell chemotaxis / RING-type E3 ubiquitin transferase / ubiquitin-protein transferase activity / ubiquitin protein ligase activity / T cell differentiation in thymus / outer membrane-bounded periplasmic space / chromatin organization / endonuclease activity / histone binding / DNA recombination / adaptive immune response / sequence-specific DNA binding / Hydrolases; Acting on ester bonds / periplasmic space / DNA damage response / chromatin binding / magnesium ion binding / protein homodimerization activity / DNA binding / zinc ion binding / metal ion binding / nucleus / membrane
Similarity search - Function
V(D)J recombination-activating protein 1, Zinc finger / RAG nonamer-binding domain / NBD domain profile. / Zinc finger RAG1-type profile. / V(D)J recombination-activating protein 1 / RAG1 importin-binding / RAG1 importin binding / Recombination-activation protein 1 (RAG1) nonamer-binding domain / Recombination activating protein 2 / RAG2 PHD domain ...V(D)J recombination-activating protein 1, Zinc finger / RAG nonamer-binding domain / NBD domain profile. / Zinc finger RAG1-type profile. / V(D)J recombination-activating protein 1 / RAG1 importin-binding / RAG1 importin binding / Recombination-activation protein 1 (RAG1) nonamer-binding domain / Recombination activating protein 2 / RAG2 PHD domain / V-D-J recombination activating protein 2 / Recombination activating protein 2, PHD domain / Galactose oxidase/kelch, beta-propeller / Kelch-type beta propeller / Zinc finger, C3HC4 RING-type / Zinc finger, C3HC4 type (RING finger) / Maltose/Cyclodextrin ABC transporter, substrate-binding protein / Solute-binding family 1, conserved site / Bacterial extracellular solute-binding proteins, family 1 signature. / Bacterial extracellular solute-binding protein / Bacterial extracellular solute-binding protein / Zinc finger, RING-type, conserved site / Zinc finger RING-type signature. / Ring finger / Zinc finger, FYVE/PHD-type / Zinc finger RING-type profile. / Zinc finger, RING-type / Zinc finger, RING/FYVE/PHD-type
Similarity search - Domain/homology
V(D)J recombination-activating protein 1 / V(D)J recombination-activating protein 2 / Maltose/maltodextrin-binding periplasmic protein / V(D)J recombination-activating protein 2
Similarity search - Component
Biological speciesDanio rerio (zebrafish)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.29 Å
AuthorsWu H / Liao M
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)AI125535 United States
CitationJournal: Nat Struct Mol Biol / Year: 2018
Title: DNA melting initiates the RAG catalytic pathway.
Authors: Heng Ru / Wei Mi / Pengfei Zhang / Frederick W Alt / David G Schatz / Maofu Liao / Hao Wu /
Abstract: The mechanism for initiating DNA cleavage by DDE-family enzymes, including the RAG endonuclease, which initiates V(D)J recombination, is not well understood. Here we report six cryo-EM structures of ...The mechanism for initiating DNA cleavage by DDE-family enzymes, including the RAG endonuclease, which initiates V(D)J recombination, is not well understood. Here we report six cryo-EM structures of zebrafish RAG in complex with one or two intact recombination signal sequences (RSSs), at up to 3.9-Å resolution. Unexpectedly, these structures reveal DNA melting at the heptamer of the RSSs, thus resulting in a corkscrew-like rotation of coding-flank DNA and the positioning of the scissile phosphate in the active site. Substrate binding is associated with dimer opening and a piston-like movement in RAG1, first outward to accommodate unmelted DNA and then inward to wedge melted DNA. These precleavage complexes show limited base-specific contacts of RAG at the conserved terminal CAC/GTG sequence of the heptamer, thus suggesting conservation based on a propensity to unwind. CA and TG overwhelmingly dominate terminal sequences in transposons and retrotransposons, thereby implicating a universal mechanism for DNA melting during the initiation of retroviral integration and DNA transposition.
History
DepositionMay 3, 2018-
Header (metadata) releaseMay 30, 2018-
Map releaseAug 1, 2018-
UpdateNov 6, 2024-
Current statusNov 6, 2024Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6dbv
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_7851.png

Downloads & links

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Map

FileDownload / File: emd_7851.map.gz / Format: CCP4 / Size: 27 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationRAG in complex with 12-RSS and 23-RSS substrate DNAs
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.24 Å/pix.
x 192 pix.
= 237.696 Å		1.24 Å/pix.
x 192 pix.
= 237.696 Å		1.24 Å/pix.
x 192 pix.
= 237.696 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.238 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.03 / Movie #1: 0.03
Minimum - Maximum-0.07793337 - 0.1659983
Average (Standard dev.)-0.000011664044 (±0.008742887)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions192192192
Spacing192192192
CellA=B=C: 237.69601 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.2381.2381.238
M x/y/z192192192
origin x/y/z0.0000.0000.000
length x/y/z237.696237.696237.696
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS192192192
D min/max/mean-0.0780.166-0.000

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Supplemental data

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Sample components

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Entire : RAG in complex with 12-RSS and 23-RSS substrate DNAs

EntireName: RAG in complex with 12-RSS and 23-RSS substrate DNAs
Components
  • Complex: RAG in complex with 12-RSS and 23-RSS substrate DNAs
    • Protein or peptide: Recombination activating gene 1 - MBP chimera
    • Protein or peptide: Recombination activating gene 2
    • Protein or peptide: Recombination activating gene 1 - MBP chimera
    • DNA: Forward strand of 12-RSS substrate DNA
    • DNA: Reverse strand of 12-RSS substrate DNA
    • DNA: Forward strand of 23-RSS substrate DNA
    • DNA: Reverse strand of 23-RSS substrate DNA
  • Ligand: ZINC ION
  • Ligand: CALCIUM ION

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Supramolecule #1: RAG in complex with 12-RSS and 23-RSS substrate DNAs

SupramoleculeName: RAG in complex with 12-RSS and 23-RSS substrate DNAs / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#7
Source (natural)Organism: Danio rerio (zebrafish)

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Macromolecule #1: Recombination activating gene 1 - MBP chimera

MacromoleculeName: Recombination activating gene 1 - MBP chimera / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: RING-type E3 ubiquitin transferase
Source (natural)Organism: Danio rerio (zebrafish)
Molecular weightTheoretical: 131.188062 KDa
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: MGSSHHHHHH GTKTEEGKLV IWINGDKGYN GLAEVGKKFE KDTGIKVTVE HPDKLEEKFP QVAATGDGPD IIFWAHDRFG GYAQSGLLA EITPDKAFQD KLYPFTWDAV RYNGKLIAYP IAVEALSLIY NKDLLPNPPK TWEEIPALDK ELKAKGKSAL M FNLQEPYF ...String:
MGSSHHHHHH GTKTEEGKLV IWINGDKGYN GLAEVGKKFE KDTGIKVTVE HPDKLEEKFP QVAATGDGPD IIFWAHDRFG GYAQSGLLA EITPDKAFQD KLYPFTWDAV RYNGKLIAYP IAVEALSLIY NKDLLPNPPK TWEEIPALDK ELKAKGKSAL M FNLQEPYF TWPLIAADGG YAFKYENGKY DIKDVGVDNA GAKAGLTFLV DLIKNKHMNA DTDYSIAEAA FNKGETAMTI NG PWAWSNI DTSKVNYGVT VLPTFKGQPS KPFVGVLSAG INAASPNKEL AKEFLENYLL TDEGLEAVNK DKPLGAVALK SYE EELAKD PRIAATMENA QKGEIMPNIP QMSAFWYAVR TAVINAASGR QTVDEALKDA QTGTDYDIPT TLEVLFQGPL GSRC QRDHL STKLIPTEVP ADLIRAVTCQ VCDHLLSDPV QSPCRHLFCR LCIIRYTHAL GPNCPTCNQH LNPSHLIKPA KFFLA TLSS LPLLCPSEEC SDWVRLDSFR EHCLNHYREK ESQEEQTPSE QNLDGYLPVN KGGRPRQHLL SLTRRAQKHR LRDLKN QVK TFAEKEEGGD VKSVCLTLFL LALRAGNEHR QADELEAMMQ GRGFGLHPAV CLAIRVNTFL SCSQYHKMYR TVKATSG RQ IFQPLHTLRN AEKELLPGFH QFEWQPALKN VSTSWDVGII DGLSGWTVSV DDVPADTISR RFRYDVALVS ALKDLEED I MEGLRERALD DSMCTSGFTV VVKESCDGMG DVSEKHGSGP AVPEKAVRFS FTIMSISIRL EGEDDGITIF QEQKPNSEL SCRPLCLMFV DESDHETLTA ILGPVVAERK AMMESRLIIS VGGLLRSFRF FFRGTGYDEK MVREMEGLEA SGSTYICTLC DSTRAEASQ NMVLHSITRS HDENLERYEI WRKNPFSESA DELRDRVKGV SAKPFMETQP TLDALHCDIG NATEFYKIFQ D EIGEVYQK PNPSREERRR WRSTLDKQLR KKMKLKPVMR MNGNYARRLM TREAVEAVCE LVPSEERREA LLKLMDLYLQ MK PVWRSTC PSRDCPDQLC QYSYNSQQFA DLLSSMFKYR YDGKITNYLH KTLAHVPEIV ERDGSIGAWA SEGNESGNKL FRR FRKMNA RQSKTFELED ILKHHWLYTS KYLQKFMEAH KNS

UniProtKB: Maltose/maltodextrin-binding periplasmic protein, V(D)J recombination-activating protein 1

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Macromolecule #2: Recombination activating gene 2

MacromoleculeName: Recombination activating gene 2 / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Danio rerio (zebrafish)
Molecular weightTheoretical: 59.43593 KDa
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: GGSMSLQPLT AVNCGSLVQP GFSLLDLEGD VYLFGQKGWP KRSCPTGIFG VRIKKGELKL RAISFSNNSS YLPPLRCPAI AHFEAQDGK PECYLIHGGR TPNNELSSSL YMLSVDSRGC NRKVTLRCEE KELVGDVPSA RYGHTLSVIN SRGKTACVLF G GRSYMPPT ...String:
GGSMSLQPLT AVNCGSLVQP GFSLLDLEGD VYLFGQKGWP KRSCPTGIFG VRIKKGELKL RAISFSNNSS YLPPLRCPAI AHFEAQDGK PECYLIHGGR TPNNELSSSL YMLSVDSRGC NRKVTLRCEE KELVGDVPSA RYGHTLSVIN SRGKTACVLF G GRSYMPPT ERTTQNWNSV VDCPPQVYLI DLEFGCCTAH TLPELTDGQS FHVALARQDC VYFLGGHILS SDCRPSRLIR LH VELLLGS PVLTCTILHE GLTITSAIAS PIGYHEYIIF GGYQSETQKR MECTYVGLDD VGVHMESREP PQWTSEISHS RTW FGGSLG KGTALVAIPS EGNPTPPEAY HFYQVSFQKE QDGEATAQGG SQESTDFEDS APLEDSEELY FGREPHELEY SSDV EGDTY NEEDEEDESQ TGYWIKCCLS CQVDPNIWEP YYSTELTRPA MIFCSRGEGG HWVHAQCMEL PESLLLQLSQ DNSKY FCLD HGGLPKQEMT PPKQMLPVKR VPMKMTHRKA PVSLKMTPAK KTFLRRLFD

UniProtKB: V(D)J recombination-activating protein 2

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Macromolecule #3: Recombination activating gene 1 - MBP chimera

MacromoleculeName: Recombination activating gene 1 - MBP chimera / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: RING-type E3 ubiquitin transferase
Source (natural)Organism: Danio rerio (zebrafish)
Molecular weightTheoretical: 131.160047 KDa
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: MGSSHHHHHH GTKTEEGKLV IWINGDKGYN GLAEVGKKFE KDTGIKVTVE HPDKLEEKFP QVAATGDGPD IIFWAHDRFG GYAQSGLLA EITPDKAFQD KLYPFTWDAV RYNGKLIAYP IAVEALSLIY NKDLLPNPPK TWEEIPALDK ELKAKGKSAL M FNLQEPYF ...String:
MGSSHHHHHH GTKTEEGKLV IWINGDKGYN GLAEVGKKFE KDTGIKVTVE HPDKLEEKFP QVAATGDGPD IIFWAHDRFG GYAQSGLLA EITPDKAFQD KLYPFTWDAV RYNGKLIAYP IAVEALSLIY NKDLLPNPPK TWEEIPALDK ELKAKGKSAL M FNLQEPYF TWPLIAADGG YAFKYENGKY DIKDVGVDNA GAKAGLTFLV DLIKNKHMNA DTDYSIAEAA FNKGETAMTI NG PWAWSNI DTSKVNYGVT VLPTFKGQPS KPFVGVLSAG INAASPNKEL AKEFLENYLL TDEGLEAVNK DKPLGAVALK SYE EELAKD PRIAATMENA QKGEIMPNIP QMSAFWYAVR TAVINAASGR QTVDEALKDA QTGTDYDIPT TLEVLFQGPL GSRC QRDHL STKLIPTEVP ADLIRAVTCQ VCDHLLSDPV QSPCRHLFCR LCIIRYTHAL GPNCPTCNQH LNPSHLIKPA KFFLA TLSS LPLLCPSEEC SDWVRLDSFR EHCLNHYREK ESQEEQTPSE QNLDGYLPVN KGGRPRQHLL SLTRRAQKHR LRDLKN QVK TFAEKEEGGD VKSVCLTLFL LALRAGNEHK QADELEAMMQ GRGFGLHPAV CLAIRVNTFL SCSQYHKMYR TVKATSG RQ IFQPLHTLRN AEKELLPGFH QFEWQPALKN VSTSWDVGII DGLSGWTVSV DDVPADTISR RFRYDVALVS ALKDLEED I MEGLRERALD DSMCTSGFTV VVKESCDGMG DVSEKHGSGP AVPEKAVRFS FTIMSISIRL EGEDDGITIF QEQKPNSEL SCRPLCLMFV DESDHETLTA ILGPVVAERK AMMESRLIIS VGGLLRSFRF FFRGTGYDEK MVREMEGLEA SGSTYICTLC DSTRAEASQ NMVLHSITRS HDENLERYEI WRKNPFSESA DELRDRVKGV SAKPFMETQP TLDALHCDIG NATEFYKIFQ D EIGEVYQK PNPSREERRR WRSTLDKQLR KKMKLKPVMR MNGNYARRLM TREAVEAVCE LVPSEERREA LLKLMDLYLQ MK PVWRSTC PSRDCPDQLC QYSYNSQQFA DLLSSMFKYR YDGKITNYLH KTLAHVPEIV ERDGSIGAWA SEGNESGNKL FRR FRKMNA RQSKTFELED ILKHHWLYTS KYLQKFMEAH KNS

UniProtKB: Maltose/maltodextrin-binding periplasmic protein, V(D)J recombination-activating protein 1

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Macromolecule #4: Forward strand of 12-RSS substrate DNA

MacromoleculeName: Forward strand of 12-RSS substrate DNA / type: dna / ID: 4 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Danio rerio (zebrafish)
Molecular weightTheoretical: 15.364879 KDa
SequenceString:
(DG)(DA)(DT)(DC)(DT)(DG)(DG)(DC)(DC)(DT) (DG)(DT)(DC)(DT)(DT)(DA)(DC)(DA)(DC)(DA) (DG)(DT)(DG)(DC)(DT)(DA)(DC)(DA)(DG) (DA)(DC)(DT)(DG)(DG)(DA)(DA)(DC)(DA)(DA) (DA) (DA)(DA)(DC)(DC)(DC)(DT)(DG)(DC) (DA)(DG)

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Macromolecule #5: Reverse strand of 12-RSS substrate DNA

MacromoleculeName: Reverse strand of 12-RSS substrate DNA / type: dna / ID: 5 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Danio rerio (zebrafish)
Molecular weightTheoretical: 15.43988 KDa
SequenceString:
(DC)(DT)(DG)(DC)(DA)(DG)(DG)(DG)(DT)(DT) (DT)(DT)(DT)(DG)(DT)(DT)(DC)(DC)(DA)(DG) (DT)(DC)(DT)(DG)(DT)(DA)(DG)(DC)(DA) (DC)(DT)(DG)(DT)(DG)(DT)(DA)(DA)(DG)(DA) (DC) (DA)(DG)(DG)(DC)(DC)(DA)(DG)(DA) (DT)(DC)

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Macromolecule #6: Forward strand of 23-RSS substrate DNA

MacromoleculeName: Forward strand of 23-RSS substrate DNA / type: dna / ID: 6 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Danio rerio (zebrafish)
Molecular weightTheoretical: 18.731996 KDa
SequenceString: (DG)(DA)(DT)(DC)(DT)(DG)(DG)(DC)(DC)(DT) (DG)(DT)(DC)(DT)(DT)(DA)(DC)(DA)(DC)(DA) (DG)(DT)(DG)(DG)(DT)(DA)(DG)(DT)(DA) (DC)(DT)(DC)(DC)(DA)(DC)(DT)(DG)(DT)(DC) (DT) (DG)(DG)(DC)(DT)(DG)(DT) ...String:
(DG)(DA)(DT)(DC)(DT)(DG)(DG)(DC)(DC)(DT) (DG)(DT)(DC)(DT)(DT)(DA)(DC)(DA)(DC)(DA) (DG)(DT)(DG)(DG)(DT)(DA)(DG)(DT)(DA) (DC)(DT)(DC)(DC)(DA)(DC)(DT)(DG)(DT)(DC) (DT) (DG)(DG)(DC)(DT)(DG)(DT)(DA)(DC) (DA)(DA)(DA)(DA)(DA)(DC)(DC)(DC)(DT)(DG) (DC)(DA) (DG)

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Macromolecule #7: Reverse strand of 23-RSS substrate DNA

MacromoleculeName: Reverse strand of 23-RSS substrate DNA / type: dna / ID: 7 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Danio rerio (zebrafish)
Molecular weightTheoretical: 18.870094 KDa
SequenceString: (DC)(DT)(DG)(DC)(DA)(DG)(DG)(DG)(DT)(DT) (DT)(DT)(DT)(DG)(DT)(DA)(DC)(DA)(DG)(DC) (DC)(DA)(DG)(DA)(DC)(DA)(DG)(DT)(DG) (DG)(DA)(DG)(DT)(DA)(DC)(DT)(DA)(DC)(DC) (DA) (DC)(DT)(DG)(DT)(DG)(DT) ...String:
(DC)(DT)(DG)(DC)(DA)(DG)(DG)(DG)(DT)(DT) (DT)(DT)(DT)(DG)(DT)(DA)(DC)(DA)(DG)(DC) (DC)(DA)(DG)(DA)(DC)(DA)(DG)(DT)(DG) (DG)(DA)(DG)(DT)(DA)(DC)(DT)(DA)(DC)(DC) (DA) (DC)(DT)(DG)(DT)(DG)(DT)(DA)(DA) (DG)(DA)(DC)(DA)(DG)(DG)(DC)(DC)(DA)(DG) (DA)(DT) (DC)

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Macromolecule #8: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 8 / Number of copies: 2 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #9: CALCIUM ION

MacromoleculeName: CALCIUM ION / type: ligand / ID: 9 / Number of copies: 4 / Formula: CA
Molecular weightTheoretical: 40.078 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
Component:
ConcentrationFormulaName
20.0 mMC8H18N2O4SHEPES
50.0 mMNaClsodium chloride
1.0 mMC9H15O6PTCEP
10.0 mMC6H14N2O2L-Lysine

Details: Solutions were made fresh from concentrated to avoid microbial contamination.
VitrificationCryogen name: ETHANE
DetailsThis sample was monodisperse.

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Electron microscopy

MicroscopeFEI POLARA 300
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 47.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Tecnai Polara / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 4.29 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 45159
Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: PROJECTION MATCHING

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