Type III secretion system, PrgH/EprH / Type III secretion system lipoprotein HrcJ/YscJ / Type III secretion system, PrgH/EprH-like / Type III secretion system protein PrgH-EprH (PrgH) / Lipoprotein YscJ/Flagellar M-ring protein / Secretory protein of YscJ/FliF family / Flagellar M-ring , N-terminal / AMP-binding enzyme, C-terminal domain superfamily / Prokaryotic membrane lipoprotein lipid attachment site profile. 類似検索 - ドメイン・相同性
ジャーナル: Science / 年: 2011 タイトル: Three-dimensional model of Salmonella's needle complex at subnanometer resolution. 著者: Oliver Schraidt / Thomas C Marlovits / 要旨: Type III secretion systems (T3SSs) are essential virulence factors used by many Gram-negative bacteria to inject proteins that make eukaryotic host cells accessible to invasion. The T3SS core ...Type III secretion systems (T3SSs) are essential virulence factors used by many Gram-negative bacteria to inject proteins that make eukaryotic host cells accessible to invasion. The T3SS core structure, the needle complex (NC), is a ~3.5 megadalton-sized, oligomeric, membrane-embedded complex. Analyzing cryo-electron microscopy images of top views of NCs or NC substructures from Salmonella typhimurium revealed a 24-fold symmetry for the inner rings and a 15-fold symmetry for the outer rings, giving an overall C3 symmetry. Local refinement and averaging showed the organization of the central core and allowed us to reconstruct a subnanometer composite structure of the NC, which together with confident docking of atomic structures reveal insights into its overall organization and structural requirements during assembly.
ダウンロード / ファイル: emd_1874.map.gz / 形式: CCP4 / 大きさ: 100.6 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
This in an image of a surface rendered, extracted volume of the IR1 from the needle complex from Salmonella typhimurium
ボクセルのサイズ
X=Y=Z: 1.33 Å
密度
表面レベル
登録者による: 0.076 / ムービー #1: 0.076
最小 - 最大
-0.18737172 - 0.21286634
平均 (標準偏差)
0.0002095 (±0.01604983)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
-150
-150
-150
サイズ
300
300
300
Spacing
300
300
300
セル
A=B=C: 399.0 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
1.33
1.33
1.33
M x/y/z
300
300
300
origin x/y/z
0.000
0.000
0.000
length x/y/z
399.000
399.000
399.000
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
-160
-160
-159
NX/NY/NZ
320
320
320
MAP C/R/S
1
2
3
start NC/NR/NS
-150
-150
-150
NC/NR/NS
300
300
300
D min/max/mean
-0.187
0.213
0.000
-
添付データ
-
試料の構成要素
-
全体 : Needle complex of the type III secretion system from Salmonella t...
全体
名称: Needle complex of the type III secretion system from Salmonella typhimurium - periplasmic domain of the inner membrane associated ring IR1
要素
試料: Needle complex of the type III secretion system from Salmonella typhimurium - periplasmic domain of the inner membrane associated ring IR1
タンパク質・ペプチド: Protein PrgH
タンパク質・ペプチド: Protein PrgK
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超分子 #1000: Needle complex of the type III secretion system from Salmonella t...
超分子
名称: Needle complex of the type III secretion system from Salmonella typhimurium - periplasmic domain of the inner membrane associated ring IR1 タイプ: sample / ID: 1000 / 集合状態: 1 / Number unique components: 2
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分子 #1: Protein PrgH
分子
名称: Protein PrgH / タイプ: protein_or_peptide / ID: 1 / Name.synonym: Protein PrgH / 集合状態: 24mer / 組換発現: No
Protocol: Rigid Body. Based on the homolog EscJ (PDB entry above) a model was built for PrgK (Swissmodel) which was subsequently used for the fitting experiment (rigid body, Chimera), each chain was fitted separately
精密化
空間: REAL / プロトコル: RIGID BODY FIT / 当てはまり具合の基準: Correlation
得られたモデル
PDB-2y9j: THREE-DIMENSIONAL MODEL OF SALMONELLA'S NEEDLE COMPLEX AT SUBNANOMETER RESOLUTION