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- PDB-3gr1: Periplasmic domain of the T3SS inner membrane protein PrgH from S... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3gr1 | ||||||
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Title | Periplasmic domain of the T3SS inner membrane protein PrgH from S.typhimurium (fragment 170-392) | ||||||
![]() | Protein prgH | ||||||
![]() | MEMBRANE PROTEIN / Type III secretion system / inner membrane protein / Cell membrane / Membrane / Transmembrane / Virulence | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Yip, C.K. / Vockovic, M. / Yu, A.C. / Strynadka, N.C.J. | ||||||
![]() | ![]() Title: A conserved structural motif mediates formation of the periplasmic rings in the type III secretion system. Authors: Spreter, T. / Yip, C.K. / Sanowar, S. / Andre, I. / Kimbrough, T.G. / Vuckovic, M. / Pfuetzner, R.A. / Deng, W. / Yu, A.C. / Finlay, B.B. / Baker, D. / Miller, S.I. / Strynadka, N.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 310.5 KB | Display | ![]() |
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PDB format | ![]() | 255.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 486.1 KB | Display | ![]() |
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Full document | ![]() | 527.1 KB | Display | |
Data in XML | ![]() | 54.8 KB | Display | |
Data in CIF | ![]() | 73.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3gr0SC ![]() 3gr5C S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
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Components
#1: Protein | Mass: 26472.988 Da / Num. of mol.: 8 / Fragment: PrgH periplasmic domain (170-392) Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.71 Å3/Da / Density % sol: 54.61 % |
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Crystal grow | Method: vapor diffusion, hanging drop / pH: 8 Details: 16% (w/v) PEG 3350 + 0.2 M tri-ammonium citrate + 0.1M Tris pH 8.0, VAPOR DIFFUSION, HANGING DROP |
-Data collection
Diffraction source | Source: ![]() ![]() ![]() |
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Detector | Type: ADSC QUANTUM 315 / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 2.8→152.5 Å / Num. obs: 55188 / % possible obs: 96.5 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3GR0 Resolution: 2.8→152.5 Å / Cor.coef. Fo:Fc: 0.907 / Cor.coef. Fo:Fc free: 0.873 / SU ML: 0.326 / ESU R: 1.843 / ESU R Free: 0.417
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Displacement parameters | Biso mean: 48.1 Å2
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Refinement step | Cycle: LAST / Resolution: 2.8→152.5 Å
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