+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-14351 | ||||||||||||
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タイトル | Structure of the human CCAN CENP-A alpha-satellite complex | ||||||||||||
マップデータ | Local resolution filtered cryosparc homogeneous refinement map | ||||||||||||
試料 |
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機能・相同性 | 機能・相同性情報 positive regulation of protein localization to kinetochore / FANCM-MHF complex / Mis6-Sim4 complex / centromere complex assembly / kinetochore organization / Fanconi anaemia nuclear complex / spindle attachment to meiosis I kinetochore / metaphase chromosome alignment / kinetochore binding / centromeric DNA binding ...positive regulation of protein localization to kinetochore / FANCM-MHF complex / Mis6-Sim4 complex / centromere complex assembly / kinetochore organization / Fanconi anaemia nuclear complex / spindle attachment to meiosis I kinetochore / metaphase chromosome alignment / kinetochore binding / centromeric DNA binding / resolution of meiotic recombination intermediates / sex differentiation / CENP-A containing chromatin assembly / chordate embryonic development / protein localization to chromosome, centromeric region / negative regulation of epithelial cell apoptotic process / kinetochore assembly / inner kinetochore / condensed chromosome, centromeric region / attachment of mitotic spindle microtubules to kinetochore / replication fork processing / mitotic sister chromatid segregation / establishment of mitotic spindle orientation / chromosome, centromeric region / mitotic cytokinesis / centriolar satellite / chromosome organization / negative regulation of megakaryocyte differentiation / protein localization to CENP-A containing chromatin / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / pericentric heterochromatin / Replacement of protamines by nucleosomes in the male pronucleus / interstrand cross-link repair / CENP-A containing nucleosome / Packaging Of Telomere Ends / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / Recognition and association of DNA glycosylase with site containing an affected purine / Cleavage of the damaged purine / Deposition of new CENPA-containing nucleosomes at the centromere / Recognition and association of DNA glycosylase with site containing an affected pyrimidine / Cleavage of the damaged pyrimidine / Resolution of Sister Chromatid Cohesion / Inhibition of DNA recombination at telomere / Meiotic synapsis / telomere organization / RNA Polymerase I Promoter Opening / NRIF signals cell death from the nucleus / Assembly of the ORC complex at the origin of replication / SUMOylation of chromatin organization proteins / DNAメチル化 / Condensation of Prophase Chromosomes / mitotic spindle organization / ERCC6 (CSB) and EHMT2 (G9a) positively regulate rRNA expression / SIRT1 negatively regulates rRNA expression / Chromatin modifications during the maternal to zygotic transition (MZT) / HCMV Late Events / innate immune response in mucosa / PRC2 methylates histones and DNA / positive regulation of protein ubiquitination / Defective pyroptosis / chromosome segregation / positive regulation of epithelial cell proliferation / RHO GTPases Activate Formins / HDACs deacetylate histones / Fanconi Anemia Pathway / RNA Polymerase I Promoter Escape / Nonhomologous End-Joining (NHEJ) / Transcriptional regulation by small RNAs / Formation of the beta-catenin:TCF transactivating complex / RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function / PKR-mediated signaling / Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 / NoRC negatively regulates rRNA expression / G2/M DNA damage checkpoint / B-WICH complex positively regulates rRNA expression / HDMs demethylate histones / DNA Damage/Telomere Stress Induced Senescence / Metalloprotease DUBs / PKMTs methylate histone lysines / 動原体 / RMTs methylate histone arginines / 遺伝的組換え / Pre-NOTCH Transcription and Translation / nuclear matrix / Activation of anterior HOX genes in hindbrain development during early embryogenesis / HCMV Early Events / Transcriptional regulation of granulopoiesis / structural constituent of chromatin / Separation of Sister Chromatids / UCH proteinases / ヌクレオソーム / antimicrobial humoral immune response mediated by antimicrobial peptide / nucleosome assembly / マイクロフィラメント / E3 ubiquitin ligases ubiquitinate target proteins / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / 染色体 / RUNX1 regulates transcription of genes involved in differentiation of HSCs / chromatin organization 類似検索 - 分子機能 | ||||||||||||
生物種 | Homo sapiens (ヒト) | ||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 12.0 Å | ||||||||||||
データ登録者 | Yatskevich S / Muir KW / Bellini D / Zhang Z / Yang J / Tischer T / Predin M / Dendooven T / McLaughlin SH / Barford D | ||||||||||||
資金援助 | 英国, ドイツ, 3件
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引用 | ジャーナル: Science / 年: 2022 タイトル: Structure of the human inner kinetochore bound to a centromeric CENP-A nucleosome. 著者: Stanislau Yatskevich / Kyle W Muir / Dom Bellini / Ziguo Zhang / Jing Yang / Thomas Tischer / Masa Predin / Tom Dendooven / Stephen H McLaughlin / David Barford / 要旨: Kinetochores assemble onto specialized centromeric CENP-A (centromere protein A) nucleosomes (CENP-A) to mediate attachments between chromosomes and the mitotic spindle. We describe cryo-electron ...Kinetochores assemble onto specialized centromeric CENP-A (centromere protein A) nucleosomes (CENP-A) to mediate attachments between chromosomes and the mitotic spindle. We describe cryo-electron microscopy structures of the human inner kinetochore constitutive centromere associated network (CCAN) complex bound to CENP-A reconstituted onto α-satellite DNA. CCAN forms edge-on contacts with CENP-A, and a linker DNA segment of the α-satellite repeat emerges from the fully wrapped end of the nucleosome to thread through the central CENP-LN channel that tightly grips the DNA. The CENP-TWSX histone-fold module further augments DNA binding and partially wraps the linker DNA in a manner reminiscent of canonical nucleosomes. Our study suggests that the topological entrapment of the linker DNA by CCAN provides a robust mechanism by which kinetochores withstand both pushing and pulling forces exerted by the mitotic spindle. | ||||||||||||
履歴 |
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-構造の表示
添付画像 |
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-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_14351.map.gz | 11.8 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-14351-v30.xml emd-14351.xml | 35.6 KB 35.6 KB | 表示 表示 | EMDBヘッダ |
FSC (解像度算出) | emd_14351_fsc.xml | 6.2 KB | 表示 | FSCデータファイル |
画像 | emd_14351.png | 39.3 KB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-14351 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-14351 | HTTPS FTP |
-関連構造データ
関連構造データ | 7ywxMC 7pb4C 7pb8C 7piiC 7pknC 7r5rC 7r5sC 7r5vC 7yyhC M: このマップから作成された原子モデル C: 同じ文献を引用 (文献) |
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類似構造データ | 類似検索 - 機能・相同性F&H 検索 |
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_14351.map.gz / 形式: CCP4 / 大きさ: 18.7 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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注釈 | Local resolution filtered cryosparc homogeneous refinement map | ||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.72 Å | ||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-試料の構成要素
+全体 : CCAN-CENP-A inner centromere complex
+超分子 #1: CCAN-CENP-A inner centromere complex
+分子 #1: Centromere protein H
+分子 #2: Centromere protein I
+分子 #3: Centromere protein K
+分子 #4: Centromere protein L
+分子 #5: Centromere protein M
+分子 #6: Centromere protein N
+分子 #7: Centromere protein O
+分子 #8: Centromere protein Q
+分子 #9: Centromere protein U
+分子 #10: Centromere protein P
+分子 #11: Centromere protein R
+分子 #12: Centromere protein T
+分子 #13: Centromere protein W
+分子 #14: Centromere protein S
+分子 #15: Centromere protein X
+分子 #18: Histone H3-like centromeric protein A
+分子 #19: Histone H4
+分子 #20: Histone H2A type 1-C
+分子 #21: Histone H2B type 1-C/E/F/G/I
+分子 #22: Centromere protein C
+分子 #16: DNA (171-MER)
+分子 #17: DNA (171-MER)
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.8 |
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凍結 | 凍結剤: ETHANE |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELDBright-field microscopy / 最大 デフォーカス(公称値): 2.6 µm / 最小 デフォーカス(公称値): 1.2 µm |
撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 平均電子線量: 40.0 e/Å2 |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |