+Open data
-Basic information
Entry | Database: PDB / ID: 7pb4 | ||||||
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Title | Cenp-HIK 3-protein complex | ||||||
Components |
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Keywords | CELL CYCLE / inner kinetochore | ||||||
Function / homology | Function and homology information kinetochore organization / inner kinetochore / kinetochore binding / sex differentiation / CENP-A containing chromatin assembly / kinetochore assembly / mitotic sister chromatid segregation / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation ...kinetochore organization / inner kinetochore / kinetochore binding / sex differentiation / CENP-A containing chromatin assembly / kinetochore assembly / mitotic sister chromatid segregation / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / Deposition of new CENPA-containing nucleosomes at the centromere / Resolution of Sister Chromatid Cohesion / mitotic spindle organization / chromosome segregation / RHO GTPases Activate Formins / kinetochore / Separation of Sister Chromatids / chromosome / nuclear body / nucleolus / nucleoplasm / nucleus / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.49 Å | ||||||
Authors | Bellini, D. / Yatskevich, S. / Muir, W.K. / Barford, D. | ||||||
Funding support | United Kingdom, 1items
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Citation | Journal: Science / Year: 2022 Title: Structure of the human inner kinetochore bound to a centromeric CENP-A nucleosome. Authors: Stanislau Yatskevich / Kyle W Muir / Dom Bellini / Ziguo Zhang / Jing Yang / Thomas Tischer / Masa Predin / Tom Dendooven / Stephen H McLaughlin / David Barford / Abstract: Kinetochores assemble onto specialized centromeric CENP-A (centromere protein A) nucleosomes (CENP-A) to mediate attachments between chromosomes and the mitotic spindle. We describe cryo-electron ...Kinetochores assemble onto specialized centromeric CENP-A (centromere protein A) nucleosomes (CENP-A) to mediate attachments between chromosomes and the mitotic spindle. We describe cryo-electron microscopy structures of the human inner kinetochore constitutive centromere associated network (CCAN) complex bound to CENP-A reconstituted onto α-satellite DNA. CCAN forms edge-on contacts with CENP-A, and a linker DNA segment of the α-satellite repeat emerges from the fully wrapped end of the nucleosome to thread through the central CENP-LN channel that tightly grips the DNA. The CENP-TWSX histone-fold module further augments DNA binding and partially wraps the linker DNA in a manner reminiscent of canonical nucleosomes. Our study suggests that the topological entrapment of the linker DNA by CCAN provides a robust mechanism by which kinetochores withstand both pushing and pulling forces exerted by the mitotic spindle. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7pb4.cif.gz | 155.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7pb4.ent.gz | 120.8 KB | Display | PDB format |
PDBx/mmJSON format | 7pb4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/pb/7pb4 ftp://data.pdbj.org/pub/pdb/validation_reports/pb/7pb4 | HTTPS FTP |
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-Related structure data
Related structure data | 7pb8C 7piiC 7pknC 7r5rC 7r5sC 7r5vC 7ywxC 7yyhC 6ypcS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein/peptide | Mass: 5726.729 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CENPH, ICEN35 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q9H3R5 |
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#2: Protein | Mass: 25832.582 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CENPI, FSHPRH1, ICEN19, LRPR1 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q92674 |
#3: Protein | Mass: 12496.531 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CENPK, ICEN37, FKSG14 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q9BS16 |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.51 Å3/Da / Density % sol: 51.02 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / Details: 10% PEG 8k, 100 mM imidazole pH 8 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I24 / Wavelength: 0.9795 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jan 26, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 2.49→53.5 Å / Num. obs: 11771 / % possible obs: 99.5 % / Redundancy: 3.7 % / CC1/2: 0.98 / Rmerge(I) obs: 0.13 / Net I/σ(I): 8.5 |
Reflection shell | Resolution: 2.49→2.6 Å / Rmerge(I) obs: 0.7 / Num. unique obs: 11771 / CC1/2: 0.3 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 6YPC Resolution: 2.49→47.37 Å / SU ML: 0.38 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 31.52 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 143.83 Å2 / Biso mean: 50.5043 Å2 / Biso min: 15.03 Å2 | ||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.49→47.37 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 4
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Refinement TLS params. | Method: refined / Origin x: -19.2463 Å / Origin y: -6.7088 Å / Origin z: 21.7924 Å
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Refinement TLS group |
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