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- PDB-6hix: Cryo-EM structure of the Trypanosoma brucei mitochondrial ribosom... -

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Basic information

Entry
Database: PDB / ID: 6hix
TitleCryo-EM structure of the Trypanosoma brucei mitochondrial ribosome - This entry contains the large mitoribosomal subunit
Components
  • (50S ribosomal protein ...) x 3
  • (Peptidyl-prolyl cis-trans ...) x 2
  • (Ribosomal protein ...) x 3
  • (UNK) x 13
  • (bL12m) x 3
  • 12S rRNA
  • Chaperone protein DNAj, putative
  • bl19m
  • bl20m
  • bl21m
  • bl27m
  • bl28m
  • bl31m
  • bl32m
  • bl33m
  • bl35m
  • bl36m
  • bl9m
  • mL70
  • ml100
  • ml38
  • ml40
  • ml41
  • ml42
  • ml43
  • ml46
  • ml49
  • ml52
  • ml53
  • ml63
  • ml64
  • ml67
  • ml68
  • ml69
  • ml71
  • ml72
  • ml73
  • ml74
  • ml75
  • ml76
  • ml78
  • ml79
  • ml80
  • ml81
  • ml82
  • ml84
  • ml85
  • ml86
  • ml87
  • ml88
  • ml89
  • ml90
  • ml91
  • ml93
  • ml94
  • ml95
  • ml96
  • ml97
  • ml98
  • ml99
  • ul10m
  • ul15m
  • ul22m
  • ul23m
  • ul24m
  • ul29m
  • ul30m
KeywordsRIBOSOME / mitoribosome / translation / Trypanosoma / large ribosomal subunit / 12S rRNA / ribosomal protein
Function / homology
Function and homology information


organellar large ribosomal subunit / mitochondrial mRNA editing complex / mitochondrial RNA processing / kinetoplast / nuclear lumen / mitochondrial large ribosomal subunit / ciliary plasm / mitochondrial ribosome / mitochondrial translation / post-transcriptional regulation of gene expression ...organellar large ribosomal subunit / mitochondrial mRNA editing complex / mitochondrial RNA processing / kinetoplast / nuclear lumen / mitochondrial large ribosomal subunit / ciliary plasm / mitochondrial ribosome / mitochondrial translation / post-transcriptional regulation of gene expression / cyclosporin A binding / ciliary basal body / peptidylprolyl isomerase / peptidyl-prolyl cis-trans isomerase activity / large ribosomal subunit / protein folding / large ribosomal subunit rRNA binding / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / mitochondrion / RNA binding / nucleus / metal ion binding / cytosol / cytoplasm
Similarity search - Function
: / Pentatricopeptide (PPR) repeat profile. / LIM domain / Zinc-binding domain present in Lin-11, Isl-1, Mec-3. / Zinc finger, LIM-type / LIM domain profile. / Pentatricopeptide repeat / Ribosomal protein L46, N-terminal / 39S mitochondrial ribosomal protein L46 / 39S ribosomal protein L46, mitochondrial ...: / Pentatricopeptide (PPR) repeat profile. / LIM domain / Zinc-binding domain present in Lin-11, Isl-1, Mec-3. / Zinc finger, LIM-type / LIM domain profile. / Pentatricopeptide repeat / Ribosomal protein L46, N-terminal / 39S mitochondrial ribosomal protein L46 / 39S ribosomal protein L46, mitochondrial / DnaJ domain / Rhodanese-like domain superfamily / 39S ribosomal protein L43/54S ribosomal protein L51 / Ribosomal protein L47, mitochondrial / MRP-L47 superfamily, mitochondrial / Mitochondrial 39-S ribosomal protein L47 (MRP-L47) / Phosphatidylethanolamine-binding protein, eukaryotic / PEBP-like superfamily / DnaJ molecular chaperone homology domain / dnaJ domain profile. / Chaperone J-domain superfamily / DnaJ domain / Cyclophilin-type peptidyl-prolyl cis-trans isomerase / ANL, N-terminal domain / AMP-dependent synthetase/ligase / AMP-binding enzyme / Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain profile. / Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain / Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD / Cyclophilin-like domain superfamily / Ribosomal protein/NADH dehydrogenase domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / NUDIX hydrolase-like domain superfamily / Zinc finger C2H2 superfamily / Ribosomal protein L11, N-terminal / Ribosomal protein L11, N-terminal domain / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L11, RNA binding domain / Ribosomal protein L11/L12 / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L28/L24 / Ribosomal protein L33 superfamily / Ribosomal protein L16 / : / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L21 / Ribosomal protein L17 / Ribosomal protein L17 superfamily / Ribosomal protein L17 / Ribosomal protein L21-like / L21-like superfamily / Ribosomal prokaryotic L21 protein / Ribosomal protein L24 / Ribosomal protein L3, bacterial/organelle-type / Ribosomal protein L15, bacterial-type / 50S ribosomal protein uL4 / : / Ribosomal protein L10e/L16 / Ribosomal protein L10e/L16 superfamily / Ribosomal protein L16p/L10e / Tetratricopeptide-like helical domain superfamily / Ribosomal protein L13 / Ribosomal protein L13 / Ribosomal protein L13 superfamily / Prokaryotic membrane lipoprotein lipid attachment site profile. / Ribosomal protein L25/L23 / Ribosomal protein L30, ferredoxin-like fold domain superfamily / Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A / Ribosomal protein L26/L24, KOW domain / Ribosomal protein L18e/L15P / Ribosomal L18e/L15P superfamily / Ribosomal protein L22/L17 / Ribosomal protein L22/L17 superfamily / Ribosomal protein L22p/L17e / Ribosomal protein L3 / Ribosomal protein L3 / Ribosomal protein L4/L1e / Ribosomal protein L4 domain superfamily / Ribosomal protein L4/L1 family / Alpha/Beta hydrolase fold / Translation protein SH3-like domain superfamily / Ribosomal protein L23/L15e core domain superfamily / Thioredoxin-like superfamily / Translation protein, beta-barrel domain superfamily / Ribosomal protein L2, domain 2 / Nucleotide-binding alpha-beta plait domain superfamily
Similarity search - Domain/homology
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Large ribosomal subunit protein uL4m / Uncharacterized protein / Uncharacterized protein / KOW domain-containing protein ...NICOTINAMIDE-ADENINE-DINUCLEOTIDE / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Large ribosomal subunit protein uL4m / Uncharacterized protein / Uncharacterized protein / KOW domain-containing protein / Uncharacterized protein / T. brucei spp.-specific protein / Uncharacterized protein / Chaperone protein DNAj, putative / Tetratricopeptide repeat / Large ribosomal subunit protein uL22c / Large ribosomal subunit protein bL21m / Large ribosomal subunit protein mL46 / T. brucei spp.-specific protein / Uncharacterized protein / Ribosomal protein L30 ferredoxin-like fold domain-containing protein / T. brucei spp.-specific protein / Uncharacterized protein / T. brucei spp.-specific protein / T. brucei spp.-specific protein / Uncharacterized protein / Uncharacterized protein / Mitochondrial RNA binding protein / T. brucei spp.-specific protein / T. brucei spp.-specific protein / T. brucei spp.-specific protein / Uncharacterized protein / Phosphatidylethanolamine-binding protein / Uncharacterized protein / Uncharacterized protein / Ribosomal protein S24/S35 mitochondrial conserved domain-containing protein / Large ribosomal subunit protein bL20c / Uncharacterized protein / Peptidyl-prolyl cis-trans isomerase / Uncharacterized protein / Large ribosomal subunit protein uL23m / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Mitochondrial RNA binding complex 1 subunit / Large ribosomal subunit protein uL29m / Rhodanese domain-containing protein / Uncharacterized protein / Uncharacterized protein / Large ribosomal subunit protein uL15/eL18 domain-containing protein / Ribosomal protein L9 domain-containing protein / 50S ribosomal protein L16, putative / Peptidyl-prolyl cis-trans isomerase / Uncharacterized protein / Uncharacterized protein / AB hydrolase-1 domain-containing protein / Ribosomal protein L9 domain-containing protein / 50S ribosomal protein L17, putative / Uncharacterized protein / 50S ribosomal protein L13, putative / Ribosomal protein L3 mitochondrial, putative / C2H2-type domain-containing protein / Uncharacterized protein / Large ribosomal subunit protein mL43 / Uncharacterized protein / AMP-dependent synthetase/ligase domain-containing protein / Uncharacterized protein / LIM zinc-binding domain-containing protein / 39S ribosomal protein L28, mitochondrial / Uncharacterized protein / Large ribosomal subunit protein uL11m / Uncharacterized protein
Similarity search - Component
Biological speciesTrypanosoma brucei brucei (eukaryote)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.39 Å
AuthorsRamrath, D.J.F. / Niemann, M. / Leibundgut, M. / Bieri, P. / Prange, C. / Horn, K. / Leitner, A. / Boehringer, D. / Schneider, A. / Ban, N.
Funding support Switzerland, 1items
OrganizationGrant numberCountry
Swiss National Science Foundation Switzerland
CitationJournal: Science / Year: 2018
Title: Evolutionary shift toward protein-based architecture in trypanosomal mitochondrial ribosomes.
Authors: David J F Ramrath / Moritz Niemann / Marc Leibundgut / Philipp Bieri / Céline Prange / Elke K Horn / Alexander Leitner / Daniel Boehringer / André Schneider / Nenad Ban /
Abstract: Ribosomal RNA (rRNA) plays key functional and architectural roles in ribosomes. Using electron microscopy, we determined the atomic structure of a highly divergent ribosome found in mitochondria of , ...Ribosomal RNA (rRNA) plays key functional and architectural roles in ribosomes. Using electron microscopy, we determined the atomic structure of a highly divergent ribosome found in mitochondria of , a unicellular parasite that causes sleeping sickness in humans. The trypanosomal mitoribosome features the smallest rRNAs and contains more proteins than all known ribosomes. The structure shows how the proteins have taken over the role of architectural scaffold from the rRNA: They form an autonomous outer shell that surrounds the entire particle and stabilizes and positions the functionally important regions of the rRNA. Our results also reveal the "minimal" set of conserved rRNA and protein components shared by all ribosomes that help us define the most essential functional elements.
History
DepositionAug 31, 2018Deposition site: PDBE / Processing site: PDBE
Revision 1.0Sep 26, 2018Provider: repository / Type: Initial release
Revision 1.1Nov 7, 2018Group: Data collection / Database references / Source and taxonomy
Category: citation / entity_src_gen
Item: _citation.journal_volume / _entity_src_gen.gene_src_strain / _entity_src_gen.pdbx_gene_src_scientific_name
Revision 2.0Feb 6, 2019Group: Atomic model / Data collection / Derived calculations
Category: atom_site / ndb_struct_na_base_pair ...atom_site / ndb_struct_na_base_pair / ndb_struct_na_base_pair_step / pdbx_nonpoly_scheme / pdbx_struct_conn_angle / pdbx_struct_sheet_hbond / struct_asym / struct_conf / struct_conn / struct_conn_type / struct_sheet / struct_sheet_order / struct_sheet_range / struct_site / struct_site_gen
Item: _atom_site.B_iso_or_equiv / _atom_site.Cartn_x ..._atom_site.B_iso_or_equiv / _atom_site.Cartn_x / _atom_site.Cartn_y / _atom_site.Cartn_z / _atom_site.auth_asym_id / _atom_site.auth_atom_id / _atom_site.auth_comp_id / _atom_site.auth_seq_id / _atom_site.label_atom_id / _atom_site.label_comp_id / _atom_site.label_entity_id / _atom_site.type_symbol / _pdbx_nonpoly_scheme.auth_mon_id / _pdbx_nonpoly_scheme.auth_seq_num / _pdbx_nonpoly_scheme.entity_id / _pdbx_nonpoly_scheme.mon_id / _pdbx_nonpoly_scheme.pdb_mon_id / _pdbx_nonpoly_scheme.pdb_seq_num / _pdbx_nonpoly_scheme.pdb_strand_id / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_auth_seq_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _struct_asym.entity_id

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Structure visualization

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  • Deposited structure unit
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  • Superimposition on EM map
  • EMDB-0231
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Structure viewerMolecule:
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Assembly

Deposited unit
A0: bl27m
A1: bl28m
A2: ul29m
A3: ul30m
A4: bl31m
A5: bl32m
A6: bl33m
A8: bl35m
A9: bl36m
AE: Ribosomal protein L3 mitochondrial, putative
AF: Ribosomal protein L4/L1 family, putative
AI: bl9m
AJ: ul10m
AK: Ribosomal protein L11, putative
AN: 50S ribosomal protein L13, putative
AP: ul15m
AQ: 50S ribosomal protein L16, putative
AR: 50S ribosomal protein L17, putative
AT: bl19m
AU: bl20m
AV: bl21m
AW: ul22m
AX: ul23m
AY: ul24m
Ab: ml38
Ad: ml40
Ae: ml41
Af: ml42
Ag: ml43
Aj: ml46
Al: ml49
Ao: ml52
Ap: ml53
At: ml63
Av: ml64
AB: bL12m
AC: bL12m
AD: bL12m
AG: bL12m
AA: 12S rRNA
BA: ml67
BB: ml68
BC: ml69
BD: mL70
BE: ml71
BF: ml72
BG: ml73
BH: ml74
BI: ml75
BJ: ml76
BK: Chaperone protein DNAj, putative
BL: ml78
BM: ml79
BN: ml80
BO: ml81
BP: ml82
BQ: Peptidyl-prolyl cis-trans isomerase
BR: ml84
BS: ml85
BT: ml86
BU: ml87
BV: ml88
BW: ml89
BX: ml90
BY: ml91
BZ: Peptidyl-prolyl cis-trans isomerase
Ba: ml93
Bb: ml94
Bc: ml95
Bd: ml96
Be: ml97
Bf: ml98
Bg: ml99
Bh: ml100
UA: UNK
UB: UNK
UC: UNK
UD: UNK
UE: UNK
UF: UNK
UG: UNK
UH: UNK
UI: UNK
UK: UNK
UL: UNK
UM: UNK
UN: UNK
UU: UNK
UV: UNK
UW: UNK
UX: UNK
hetero molecules


Theoretical massNumber of molelcules
Total (without water)2,560,420105
Polymers2,559,19491
Non-polymers1,22614
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: mass spectrometry
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

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Protein , 63 types, 63 molecules A0A1A2A3A4A5A6A8A9AIAJAPATAUAVAWAXAYAbAdAeAfAgAjAlAoApAtAvAB...

#1: Protein bl27m


Mass: 21793.092 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: TEOVI_000532300 / Production host: Trypanosoma brucei brucei (eukaryote) / References: UniProt: A0A1G4I8T3
#2: Protein bl28m


Mass: 28051.373 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb927.6.4040 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q586A2
#3: Protein ul29m


Mass: 54045.367 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb09.160.5240 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q38EM7
#4: Protein ul30m


Mass: 25204.334 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: TbgDal_IX4520 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZY77
#5: Protein bl31m


Mass: 21083.229 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb10.6k15.1920 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q38AB0
#6: Protein bl32m


Mass: 9745.710 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb927.4.2330 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q584F4
#7: Protein bl33m


Mass: 13042.740 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb927.4.1810 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q581Q0
#8: Protein bl35m


Mass: 21661.453 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: TbgDal_X2260 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: D0A1K1
#9: Protein bl36m


Mass: 21795.227 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: TbgDal_IV4820 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZMA9
#12: Protein bl9m


Mass: 31032.697 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb927.5.3410 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q57UC5
#13: Protein ul10m


Mass: 20283.566 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 106-129 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: TbgDal_V3970 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZPD1
#16: Protein ul15m


Mass: 43780.016 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb927.5.3980 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q57U68
#19: Protein bl19m


Mass: 16931.455 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: TB927.1.1210 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q4GZ98
#20: Protein bl20m


Mass: 24393.943 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb11.01.1930 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q383R2
#21: Protein bl21m


Mass: 20895.268 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: TbgDal_VII4650 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZT11
#22: Protein ul22m


Mass: 32083.285 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: TbgDal_VII3160 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZSI8
#23: Protein ul23m


Mass: 28822.936 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb11.03.0260 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q387G3
#24: Protein ul24m


Mass: 43999.047 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 313-340 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: TbgDal_III1550 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZK52
#25: Protein ml38


Mass: 58437.895 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 41-53 and 294-311 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb11.01.6620 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q381T7
#26: Protein ml40


Mass: 33646.066 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: TbgDal_X14530 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: D0A511
#27: Protein ml41


Mass: 22556.877 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: TbgDal_XI11060 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: D0A8I6
#28: Protein ml42


Mass: 21602.639 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb11.01.1840 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q383S1
#29: Protein ml43


Mass: 31300.406 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb927.4.4600 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q583E5
#30: Protein ml46


Mass: 33984.199 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 149-159 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: TbgDal_VII5370 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZT91
#31: Protein ml49


Mass: 24501.486 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 102-109 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb927.5.3110 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q57Z82
#32: Protein ml52


Mass: 29175.984 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb11.02.2250 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q385V2
#33: Protein ml53


Mass: 35656.383 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb927.7.2990, Tb927.7.3030 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q57YA9
#34: Protein ml63


Mass: 18213.924 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 64-73 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: TbgDal_VII8230 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZU82
#35: Protein ml64


Mass: 28545.977 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb11.01.3500 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q383B7
#36: Protein bL12m


Mass: 3998.370 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427
#40: Protein ml67


Mass: 93569.273 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: TbgDal_XI1730 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: D0A5V6
#41: Protein ml68


Mass: 62806.293 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 264-343 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: TbgDal_X580 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: D0A135
#42: Protein ml69


Mass: 59010.855 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb927.6.2010 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q584V5
#43: Protein mL70


Mass: 62442.410 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427
#44: Protein ml71


Mass: 50313.797 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb927.7.3460 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q57WG1
#45: Protein ml72


Mass: 49407.000 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: TbgDal_VI3710 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZR63
#46: Protein ml73


Mass: 41857.875 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb927.7.6800 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q57Y49
#47: Protein ml74


Mass: 39765.176 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb10.6k15.3290 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q38AM5
#48: Protein ml75


Mass: 39841.617 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb10.70.7960 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q38CK0
#49: Protein ml76


Mass: 39638.914 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 168-176 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb11.01.2340 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q383M2
#50: Protein Chaperone protein DNAj, putative


Mass: 43819.699 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 248-281 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: TbgDal_VI2240 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZQR6
#51: Protein ml78


Mass: 35104.535 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: TbgDal_X13360 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: D0A4P5
#52: Protein ml79


Mass: 33079.617 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb11.01.7140 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q381N5
#53: Protein ml80


Mass: 34883.273 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 145-174 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb927.6.1440 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q585A3
#54: Protein ml81


Mass: 29284.467 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: TbgDal_XI6240 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: D0A755
#55: Protein ml82


Mass: 29283.676 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 153-162 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: TbgDal_X19420 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: D0A0S6
#57: Protein ml84


Mass: 24773.643 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb927.6.4080 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q586A6
#58: Protein ml85


Mass: 22657.951 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb09.160.2250 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q38FG8
#59: Protein ml86


Mass: 22376.191 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: TbgDal_V5660 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZPU8
#60: Protein ml87


Mass: 22337.521 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: TbgDal_XI9190 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: D0A7Z9
#61: Protein ml88


Mass: 22255.590 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: TbgDal_VIII1510 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: C9ZUW3
#62: Protein ml89


Mass: 22213.406 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb927.3.820 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q57WW5
#63: Protein ml90


Mass: 22123.414 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb927.6.1700 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q585P1
#64: Protein ml91


Mass: 20618.520 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb927.3.5300 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q580U5
#66: Protein ml93


Mass: 18929.691 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: TbgDal_X13710 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: D0A4T0
#67: Protein ml94


Mass: 17830.959 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb09.160.5050 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q38EP7
#68: Protein ml95


Mass: 17803.377 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb10.26.0585 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q389K3
#69: Protein ml96


Mass: 16387.654 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb11.01.6285 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q381W9
#70: Protein ml97


Mass: 12974.785 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb10.61.3115 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q388L8
#71: Protein ml98


Mass: 12968.644 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb10.61.3155 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q388M2
#72: Protein ml99


Mass: 12334.969 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb927.2.2590 / Production host: Trypanosoma brucei brucei (eukaryote) / References: UniProt: Q587H8
#73: Protein ml100


Mass: 10600.362 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: TEOVI_000790200 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: A0A1G4HYZ0
#77: Protein UNK


Mass: 15081.565 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: unassigned residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427

-
Ribosomal protein ... , 3 types, 3 molecules AEAFAK

#10: Protein Ribosomal protein L3 mitochondrial, putative


Mass: 55073.699 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb927.3.5610 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q580R4
#11: Protein Ribosomal protein L4/L1 family, putative


Mass: 53156.664 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: TEOVI_000582800 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: A0A1G4HYD1
#14: Protein Ribosomal protein L11, putative


Mass: 40252.750 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb927.2.4740, Tb927.2.4890 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q586R9

-
50S ribosomal protein ... , 3 types, 3 molecules ANAQAR

#15: Protein 50S ribosomal protein L13, putative


Mass: 24341.309 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb927.4.1070 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q580D5
#17: Protein 50S ribosomal protein L16, putative


Mass: 19158.145 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb927.7.3960 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q57UM6
#18: Protein 50S ribosomal protein L17, putative


Mass: 35117.824 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb927.8.5860 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q57YI7

-
Protein/peptide , 14 types, 19 molecules ACADAGUAUBUCUHUEUFUGUNUIUKULUMUUUVUXUW

#37: Protein/peptide bL12m


Mass: 2008.194 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427
#38: Protein/peptide bL12m


Mass: 1937.116 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427
#74: Protein/peptide UNK


Mass: 3932.839 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: unassigned residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427
#75: Protein/peptide UNK


Mass: 3422.209 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: unassigned residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427
#76: Protein/peptide UNK


Mass: 1039.273 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: unassigned residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427
#78: Protein/peptide UNK


Mass: 1890.321 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: unassigned residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427
#79: Protein/peptide UNK


Mass: 2060.531 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Details: unassigned residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427
#80: Protein/peptide UNK


Mass: 1464.797 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: unassigned residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427
#81: Protein/peptide UNK


Mass: 869.063 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: unassigned residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427
#82: Protein/peptide UNK


Mass: 1294.587 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: unassigned residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427
#83: Protein/peptide UNK


Mass: 528.644 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427
#84: Protein/peptide UNK


Mass: 954.168 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: unassigned residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427
#85: Protein/peptide UNK


Mass: 698.854 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427
#86: Protein/peptide UNK


Mass: 613.749 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: unassigned residues built as UNK / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427

-
RNA chain , 1 types, 1 molecules AA

#39: RNA chain 12S rRNA


Mass: 376748.500 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: GenBank: 343546

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Peptidyl-prolyl cis-trans ... , 2 types, 2 molecules BQBZ

#56: Protein Peptidyl-prolyl cis-trans isomerase / PPIase


Mass: 25579.004 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb927.7.3430 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q57WF8, peptidylprolyl isomerase
#65: Protein Peptidyl-prolyl cis-trans isomerase / PPIase


Mass: 20542.291 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: residues 46-64 built as polyalanines / Source: (gene. exp.) Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427 / Gene: Tb11.01.1215 / Production host: Trypanosoma brucei brucei (eukaryote) / Strain (production host): Lister 427 / References: UniProt: Q383Y4, peptidylprolyl isomerase

-
Non-polymers , 3 types, 14 molecules

#87: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: Zn
#88: Chemical ChemComp-NAD / NICOTINAMIDE-ADENINE-DINUCLEOTIDE


Mass: 663.425 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C21H27N7O14P2 / Comment: NAD*YM
#89: Chemical
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 7 / Source method: obtained synthetically / Formula: Mg

-
Experimental details

-
Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

-
Sample preparation

ComponentName: large subunit of the T. brucei mitoribosome / Type: RIBOSOME / Entity ID: #1-#86 / Source: NATURAL
Molecular weightValue: 1.9 MDa / Experimental value: YES
Source (natural)Organism: Trypanosoma brucei brucei (eukaryote) / Strain: Lister 427
Buffer solutionpH: 7.4
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE-PROPANE / Humidity: 98 % / Chamber temperature: 278 K

-
Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD
Image recordingElectron dose: 40 e/Å2 / Detector mode: INTEGRATING / Film or detector model: FEI FALCON III (4k x 4k)

-
Processing

EM software
IDNameCategory
1EMANparticle selection
4CTFFINDCTF correction
10RELIONfinal Euler assignment
11RELIONclassification
12RELION3D reconstruction
13Cootmodel refinement
14PHENIXmodel refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3.39 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 31619 / Algorithm: FOURIER SPACE / Symmetry type: POINT
Atomic model buildingB value: 52.2 / Protocol: AB INITIO MODEL
Details: We used Coot and O ofr initial model building and refined the structure using PHENIX

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