EMDB-4525: Human post-catalytic spliceosome (P complex) stalled with DHX8 K5...

Basic information

• Entry: Database: EMDB / ID: EMD-4525
• Title: Human post-catalytic spliceosome (P complex) stalled with DHX8 K594A mutant, overall map
• Map data: Human post-catalytic spliceosome (P complex) stalled with DHX8 K594A mutant, overall map

Sample

• Complex: Human post-catalytic P complex spliceosome
  • Complex: Human post-catalytic P complex spliceosome
    • RNA: x 3 types
    • Protein or peptide: x 38 types
  • Complex: ATP-dependent RNA helicase DHX8
    • Protein or peptide: x 1 types
  • Complex: MINX introns and exons
    • RNA: x 2 types
• Ligand: x 6 types

Function / homology

Function:

second spliceosomal transesterification activity / exon-exon junction subcomplex mago-y14 / negative regulation of selenocysteine incorporation / regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / spliceosomal complex disassembly / cellular response to selenite ion / selenocysteine insertion sequence binding / exon-exon junction complex / pre-mRNA 3'-splice site binding / snRNP binding / granulocyte differentiation / U2 snRNP binding / post-mRNA release spliceosomal complex / U7 snRNA binding / histone pre-mRNA DCP binding / regulation of retinoic acid receptor signaling pathway / U7 snRNP / negative regulation of toll-like receptor signaling pathway / 3'-5' RNA helicase activity / regulation of translation at postsynapse, modulating synaptic transmission / histone pre-mRNA 3'end processing complex / alternative mRNA splicing, via spliceosome / renal system process / cis assembly of pre-catalytic spliceosome / generation of catalytic spliceosome for first transesterification step / negative regulation of excitatory postsynaptic potential / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / regulation of vitamin D receptor signaling pathway / Z-decay: degradation of maternal mRNAs by zygotically expressed factors / SLBP independent Processing of Histone Pre-mRNAs / SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs / negative regulation of interleukin-8 production / regulation of mRNA processing / negative regulation of lipopolysaccharide-mediated signaling pathway / spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) / Deadenylation of mRNA / embryonic brain development / protein methylation / U12-type spliceosomal complex / methylosome / negative regulation of phosphorylation / 7-methylguanosine cap hypermethylation / pre-mRNA binding / negative regulation of interferon-beta production / nuclear retinoic acid receptor binding / U2-type catalytic step 1 spliceosome / pICln-Sm protein complex / U1 snRNP binding / RNA splicing, via transesterification reactions / Prp19 complex / poly(A) binding / positive regulation of androgen receptor activity / spliceosomal tri-snRNP complex / small nuclear ribonucleoprotein complex / M-decay: degradation of maternal mRNAs by maternally stored factors / P granule / RNA stem-loop binding / sno(s)RNA-containing ribonucleoprotein complex / ATP-dependent activity, acting on RNA / SMN-Sm protein complex / mRNA 3'-end processing / mRNA cis splicing, via spliceosome / U2-type spliceosomal complex / telomerase RNA binding / embryonic cranial skeleton morphogenesis / telomerase holoenzyme complex / positive regulation of mRNA splicing, via spliceosome / U2-type precatalytic spliceosome / regulation of mRNA splicing, via spliceosome / C2H2 zinc finger domain binding / U2-type prespliceosome assembly / commitment complex / U2-type catalytic step 2 spliceosome / U4 snRNP / positive regulation by host of viral transcription / Transport of Mature mRNA derived from an Intron-Containing Transcript / positive regulation of vitamin D receptor signaling pathway / U2 snRNP / Notch binding / nuclear vitamin D receptor binding / RNA Polymerase II Transcription Termination / Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells / U1 snRNP / RUNX3 regulates NOTCH signaling / positive regulation of alpha-beta T cell differentiation / U2-type prespliceosome / NOTCH4 Intracellular Domain Regulates Transcription / K63-linked polyubiquitin modification-dependent protein binding / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / ubiquitin-ubiquitin ligase activity / exploration behavior / WD40-repeat domain binding / NOTCH3 Intracellular Domain Regulates Transcription / positive regulation of neurogenesis / lipid biosynthetic process / precatalytic spliceosome / regulation of alternative mRNA splicing, via spliceosome / hematopoietic stem cell proliferation / nuclear androgen receptor binding / cyclosporin A binding

Domain/homology:

Sde2, N-terminal ubiquitin domain / Silencing defective 2 N-terminal ubiquitin domain / NF-kappa-B-activating protein, C-terminal / NF-kappa-B-activating protein / NF-kappa-B-activating protein C-terminal domain / NF-kappa-B-activating protein / Protein FAM32A / Cactin, central domain / Cactin, C-terminal / FAM32A / Cactus-binding C-terminus of cactin protein / Conserved mid region of cactin / PRKR-interacting protein 1 / Protein of unknown function (DUF1168) / Pre-mRNA-splicing factor SLU7 domain / Pre-mRNA-splicing factor SLU7 / Pre-mRNA splicing Prp18-interacting factor / DHX8/ Prp22, DEXH-box helicase domain / Mago nashi protein / RNA-binding motif protein 8 / RBM8, RNA recognition motif / Mago nashi superfamily / Mago nashi protein / : / : / : / : / Intron-binding protein aquarius, beta-barrel / Intron-binding protein aquarius insert domain / : / Pre-mRNA-splicing factor SPF27 / Breast carcinoma amplified sequence 2 (BCAS2) / CWF11 family / Intron-binding protein aquarius, N-terminal / Intron-binding protein aquarius N-terminal / : / Replication stress response SDE2 C-terminal / Cactus-binding C-terminus of cactin protein / mRNA splicing factor SYF2 / SYF2 splicing factor / U2 small nuclear ribonucleoprotein B'', RNA recognition motif 2 / U2 small nuclear ribonucleoprotein B'', RNA recognition motif 1 / Cyclophilin-type peptidyl-prolyl cis-trans isomerase, cyclophilin A-like / mRNA splicing factor Cwf21 domain / cwf21 domain / Pre-mRNA-processing factor 17 / : / STL11, N-terminal / Pre-mRNA-splicing factor 19 / Pre-mRNA-processing factor 19 / Prp19/Pso4-like / : / U-box domain / DNA2/NAM7 helicase, helicase domain / DNA2/NAM7-like helicase / AAA domain / WD repeat Prp46/PLRG1-like / BUD31/G10-related, conserved site / : / : / G10 protein signature 1. / G10 protein signature 2. / SKI-interacting protein SKIP, SNW domain / SKI-interacting protein, SKIP / SKIP/SNW domain / Myb-like DNA-binding domain / Pre-mRNA-splicing factor Cwf15/Cwc15 / HAT (Half-A-TPR) repeat / Cwf15/Cwc15 cell cycle control protein / Small ribonucleoprotein associated, SmB/SmN / Pre-mRNA-splicing factor Cwc2/Slt11 / G10 protein / Pre-mRNA-splicing factor BUD31 / Pre-mRNA splicing factor component Cdc5p/Cef1, C-terminal / pre-mRNA splicing factor component / Initiation factor eIF-4 gamma, MA3 / MA3 domain / MI domain profile. / Domain in DAP-5, eIF4G, MA-3 and other proteins. / Small nuclear ribonucleoprotein D1 / MIF4G domain / Spt5 C-terminal domain / Spt5 C-terminal nonapeptide repeat binding Spt4 / U2A'/phosphoprotein 32 family A, C-terminal / occurring C-terminal to leucine-rich repeats / U-box domain profile. / Leucine-rich repeat / Modified RING finger domain / Pre-mRNA-splicing factor Syf1-like / Brr2, N-terminal helicase PWI domain / : / N-terminal helicase PWI domain / Pre-mRNA-splicing helicase BRR2 plug domain / Middle domain of eukaryotic initiation factor 4G (eIF4G) / MIF4G-like, type 3 / U-box domain / Snu114, GTP-binding domain / Sec63 Brl domain / Zinc finger, CCCH-type superfamily / 116kDa U5 small nuclear ribonucleoprotein component, N-terminal

Component:

Pleiotropic regulator 1 / RNA helicase aquarius / Pre-mRNA-processing factor 17 / U5 small nuclear ribonucleoprotein 200 kDa helicase / Pre-mRNA-splicing factor SPF27 / Pre-mRNA-splicing factor SLU7 / Pre-mRNA-splicing factor SYF2 / U2 small nuclear ribonucleoprotein B'' / U2 small nuclear ribonucleoprotein A' / Small nuclear ribonucleoprotein-associated proteins B and B' / Eukaryotic initiation factor 4A-III / Protein BUD31 homolog / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein F / Small nuclear ribonucleoprotein G / Small nuclear ribonucleoprotein Sm D1 / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein Sm D3 / SNW domain-containing protein 1 / ATP-dependent RNA helicase DHX8 / 116 kDa U5 small nuclear ribonucleoprotein component / Splicing regulator SDE2 / Pre-mRNA-processing-splicing factor 8 / NF-kappa-B-activating protein / Splicing factor Cactin / Protein mago nashi homolog 2 / U5 small nuclear ribonucleoprotein 40 kDa protein / Cell division cycle 5-like protein / Crooked neck-like protein 1 / PRKR-interacting protein 1 / Pre-mRNA-splicing factor CWC22 homolog / Pre-mRNA-splicing factor SYF1 / Pre-mRNA-splicing factor RBM22 / Spliceosome-associated protein CWC15 homolog / Pre-mRNA-processing factor 19 / Serine/arginine repetitive matrix protein 2 / Peptidyl-prolyl cis-trans isomerase-like 1 / Protein FAM32A / RNA-binding protein 8A

• Biological species: Homo sapiens (human) / Human adenovirus 2 / Human (human)
• Method: single particle reconstruction / cryo EM / Resolution: 3.3 Å
• Authors: Fica SM / Oubridge C / Wilkinson ME / Newman AJ / Nagai K

Funding support

United Kingdom, 2 items

Organization	Grant number	Country
Medical Research Council (United Kingdom)	MC_U105184330	United Kingdom
European Research Council	AdG-693087-SPLICE3D

• Citation: Journal: Science / Year: 2019; Title: A human postcatalytic spliceosome structure reveals essential roles of metazoan factors for exon ligation.; Authors: Sebastian M Fica / Chris Oubridge / Max E Wilkinson / Andrew J Newman / Kiyoshi Nagai / ; Abstract: During exon ligation, the spliceosome recognizes the 3'-splice site (3'SS) of precursor messenger RNA (pre-mRNA) through non-Watson-Crick pairing with the 5'SS and the branch adenosine, in a conformation stabilized by Prp18 and Prp8. Here we present the 3.3-angstrom cryo-electron microscopy structure of a human postcatalytic spliceosome just after exon ligation. The 3'SS docks at the active site through conserved RNA interactions in the absence of Prp18. Unexpectedly, the metazoan-specific FAM32A directly bridges the 5'-exon and intron 3'SS of pre-mRNA and promotes exon ligation, as shown by functional assays. CACTIN, SDE2, and NKAP-factors implicated in alternative splicing-further stabilize the catalytic conformation of the spliceosome during exon ligation. Together these four proteins act as exon ligation factors. Our study reveals how the human spliceosome has co-opted additional proteins to modulate a conserved RNA-based mechanism for 3'SS selection and to potentially fine-tune alternative splicing at the exon ligation stage.

History

Deposition	Jan 3, 2019	-
Header (metadata) release	Feb 20, 2019	-
Map release	Feb 20, 2019	-
Update	Oct 7, 2020	-
Current status	Oct 7, 2020	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_4525.map.gz / Format: CCP4 / Size: 262.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Human post-catalytic spliceosome (P complex) stalled with DHX8 K594A mutant, overall map
• Voxel size: X=Y=Z: 1.2 Å

Density

Contour Level	By AUTHOR: 0.024 / Movie #1: 0.024
Minimum - Maximum	-0.10098974 - 0.17720254
Average (Standard dev.)	0.00007733217 (±0.0050888886)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 410 410 410 Spacing 410 410 410
Cell	A=B=C: 492.00003 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.2	1.2	1.2
M x/y/z	410	410	410
origin x/y/z	0.000	0.000	0.000
length x/y/z	492.000	492.000	492.000
α/β/γ	90.000	90.000	90.000
start NX/NY/NZ	0	0	0
NX/NY/NZ	364	364	364
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	410	410	410
D min/max/mean	-0.101	0.177	0.000

Supplemental data

Sample components

Entire : Human post-catalytic P complex spliceosome

• Entire: Name: Human post-catalytic P complex spliceosome

Components

• Complex: Human post-catalytic P complex spliceosome
  • Complex: Human post-catalytic P complex spliceosome
    • RNA: U2 snRNAU2 spliceosomal RNA
    • RNA: U5 snRNAU5 spliceosomal RNA
    • RNA: U6 snRNAU6 spliceosomal RNA
    • Protein or peptide: Eukaryotic initiation factor 4A-III
    • Protein or peptide: RNA-binding protein 8A
    • Protein or peptide: Protein mago nashi homolog 2
    • Protein or peptide: Pre-mRNA-processing-splicing factor 8
    • Protein or peptide: U5 small nuclear ribonucleoprotein 200 kDa helicase
    • Protein or peptide: 116 kDa U5 small nuclear ribonucleoprotein component
    • Protein or peptide: PRKR-interacting protein 1
    • Protein or peptide: Cactin
    • Protein or peptide: Protein FAM32A
    • Protein or peptide: Pre-mRNA-splicing factor CWC22 homolog
    • Protein or peptide: Pleiotropic regulator 1
    • Protein or peptide: SNW domain-containing protein 1
    • Protein or peptide: Protein BUD31 homolog
    • Protein or peptide: Pre-mRNA-splicing factor RBM22
    • Protein or peptide: U5 small nuclear ribonucleoprotein 40 kDa protein
    • Protein or peptide: Cell division cycle 5-like protein
    • Protein or peptide: Spliceosome-associated protein CWC15 homolog
    • Protein or peptide: Serine/arginine repetitive matrix protein 2
    • Protein or peptide: Crooked neck-like protein 1
    • Protein or peptide: Pre-mRNA-splicing factor SYF1
    • Protein or peptide: Intron-binding protein aquarius
    • Protein or peptide: U2 small nuclear ribonucleoprotein A'
    • Protein or peptide: U2 small nuclear ribonucleoprotein B''
    • Protein or peptide: NF-kappa-B-activating protein
    • Protein or peptide: Small nuclear ribonucleoprotein-associated proteins B and B'
    • Protein or peptide: Pre-mRNA-splicing factor SLU7
    • Protein or peptide: Small nuclear ribonucleoprotein Sm D3
    • Protein or peptide: Small nuclear ribonucleoprotein E
    • Protein or peptide: Small nuclear ribonucleoprotein F
    • Protein or peptide: Small nuclear ribonucleoprotein G
    • Protein or peptide: Small nuclear ribonucleoprotein Sm D1
    • Protein or peptide: Peptidyl-prolyl cis-trans isomerase-like 1
    • Protein or peptide: Small nuclear ribonucleoprotein Sm D2
    • Protein or peptide: Pre-mRNA-processing factor 17
    • Protein or peptide: Pre-mRNA-splicing factor SPF27
    • Protein or peptide: Pre-mRNA-processing factor 19
    • Protein or peptide: Pre-mRNA-splicing factor SYF2
    • Protein or peptide: Replication stress response regulator SDE2
  • Complex: ATP-dependent RNA helicase DHX8
    • Protein or peptide: ATP-dependent RNA helicase DHX8
  • Complex: MINX introns and exons
    • RNA: Ligated exons: MINX mRNA
    • RNA: Intron lariat: MINX RNA
• Ligand: MAGNESIUM ION
• Ligand: POTASSIUM IONPotassium
• Ligand: ADENOSINE-5'-TRIPHOSPHATE
• Ligand: GUANOSINE-5'-TRIPHOSPHATEGuanosine triphosphate
• Ligand: ZINC ION
• Ligand: INOSITOL HEXAKISPHOSPHATEPhytic acid

Supramolecule #1: Human post-catalytic P complex spliceosome

• Supramolecule: Name: Human post-catalytic P complex spliceosome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#44
• Molecular weight: Theoretical: 3.0 MDa

Supramolecule #2: Human post-catalytic P complex spliceosome

• Supramolecule: Name: Human post-catalytic P complex spliceosome / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#10, #12-#14, #16-#26, #28-#44
• Source (natural): Organism: Homo sapiens (human)

Supramolecule #3: ATP-dependent RNA helicase DHX8

• Supramolecule: Name: ATP-dependent RNA helicase DHX8 / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #27
• Source (natural): Organism: Homo sapiens (human)
• Recombinant expression: Organism: Saccharomyces cerevisiae (brewer's yeast)

Supramolecule #4: MINX introns and exons

• Supramolecule: Name: MINX introns and exons / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #11, #15
• Source (natural): Organism: Human adenovirus 2
• Recombinant expression: Organism: synthetic construct (others)

Macromolecule #1: U2 snRNA

• Macromolecule: Name: U2 snRNA / type: rna / ID: 1 / Number of copies: 1
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 60.459621 KDa

Sequence

String:

AUCGCUUCUC GGCCUUUUGG CUAAGAUCAA GUGUAGUAUC UGUUCUUAUC AGUUUAAUAU CUGAUACGUC CUCUAUCCGA GGACAAUAU AUUAAAAGAU UUUUGGAACU CAGGGAGAUG GAAUAGGAGC UUGCUCCGUC CACUCCACGC AUCGACCUGG U AUUGCAGU ACCUCCAGGA ACGGUGCACC C

Macromolecule #2: U5 snRNA

• Macromolecule: Name: U5 snRNA / type: rna / ID: 2 / Number of copies: 1
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 36.908668 KDa

Sequence

String:

AUACUCUGGU UUCUCUUCAG AUCGCAUAAA UCUUUCGCCU UUUACUAAAG AUUUCCGUGG AGAGGAACAA CUCUGAGUCU UAACCCAAU UUUUUGAGCC UUGCCUUGGC AAGGCUA

Macromolecule #3: U6 snRNA

• Macromolecule: Name: U6 snRNA / type: rna / ID: 3 / Number of copies: 1
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 34.09827 KDa

Sequence

String:

GUGCUCGCUU CGGCAGCACA UAUACUAAAA UUGGAACGAU ACAGAGAAGA UUAGCAUGGC CCCUGCGCAA GGAUGACACG CAAAUUCGU GAAGCGUUCC AUAUUUU

Macromolecule #11: Ligated exons: MINX mRNA

• Macromolecule: Name: Ligated exons: MINX mRNA / type: rna / ID: 11 / Number of copies: 1
• Source (natural): Organism: Human adenovirus 2
• Molecular weight: Theoretical: 4.4377 KDa

Sequence

String:

GGCCUCCGAA CGCU

Macromolecule #15: Intron lariat: MINX RNA

• Macromolecule: Name: Intron lariat: MINX RNA / type: rna / ID: 15 / Number of copies: 1
• Source (natural): Organism: Human adenovirus 2
• Molecular weight: Theoretical: 27.772039 KDa

Sequence

String:

GUAAGAGCCU AGCAUGUAGA A(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)(N)(N)(N)(N)(N) (N)(N) (N)(N)(N)(N)(N)(N)(N)(N)(N)(N) (N)(N)UGAUGAUG UCAUACUUAU CCU(N)(N)(N)(N)(N) (N) (N)(N)(N)CAG

Macromolecule #4: Eukaryotic initiation factor 4A-III

• Macromolecule: Name: Eukaryotic initiation factor 4A-III / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 44.691359 KDa

Sequence

String:

DMTKVEFETS EEVDVTPTFD TMGLREDLLR GIYAYGFEKP SAIQQRAIKQ IIKGRDVIAQ SQSGTGKTAT FSISVLQCLD IQVRETQAL ILAPTRELAV QIQKGLLALG DYMNVQCHAC IGGTNVGEDI RKLDYGQHVV AGTPGRVFDM IRRRSLRTRA I KMLVLDEA DEMLNKGFKE QIYDVYRYLP PATQVVLISA TLPHEILEMT NKFMTDPIRI LVKRDELTLE GIKQFFVAVE RE EWKFDTL CDLYDTLTIT QAVIFCNTKR KVDWLTEKMR EANFTVSSMH GDMPQKERES IMKEFRSGAS RVLISTDVWA RGL DVPQVS LIINYDLPNN RELYIHRIGR SGRYGRKGVA INFVKNDDIR ILRDIEQYYS TQIDEMPMNV ADLI

Macromolecule #5: RNA-binding protein 8A

• Macromolecule: Name: RNA-binding protein 8A / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 10.370526 KDa

Sequence

String:

PGPQRSVEGW ILFVTGVHEE ATEEDIHDKF AEYGEIKNIH LNLDRRTGYL KGYTLVEYET YKEAQAAMEG LNGQDLMGQP ISVDWCFVR GP

Macromolecule #6: Protein mago nashi homolog 2

• Macromolecule: Name: Protein mago nashi homolog 2 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 16.929314 KDa

Sequence

String:

SDFYLRYYVG HKGKFGHEFL EFEFRPDGKL RYANNSNYKN DVMIRKEAYV HKSVMEELKR IIDDSEITKE DDALWPPPDR VGRQELEIV IGDEHISFTT SKIGSLIDVN QSKDPEGLRV FYYLVQDLKC LVFSLIGLHF KIKPI

Macromolecule #7: Pre-mRNA-processing-splicing factor 8

• Macromolecule: Name: Pre-mRNA-processing-splicing factor 8 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 273.97425 KDa

Sequence

String:

MAGVFPYRGP GNPVPGPLAP LPDYMSEEKL QEKARKWQQL QAKRYAEKRK FGFVDAQKED MPPEHVRKII RDHGDMTNRK FRHDKRVYL GALKYMPHAV LKLLENMPMP WEQIRDVPVL YHITGAISFV NEIPWVIEPV YISQWGSMWI MMRREKRDRR H FKRMRFPP FDDEEPPLDY ADNILDVEPL EAIQLELDPE EDAPVLDWFY DHQPLRDSRK YVNGSTYQRW QFTLPMMSTL YR LANQLLT DLVDDNYFYL FDLKAFFTSK ALNMAIPGGP KFEPLVRDIN LQDEDWNEFN DINKIIIRQP IRTEYKIAFP YLY NNLPHH VHLTWYHTPN VVFIKTEDPD LPAFYFDPLI NPISHRHSVK SQEPLPDDDE EFELPEFVEP FLKDTPLYTD NTAN GIALL WAPRPFNLRS GRTRRALDIP LVKNWYREHC PAGQPVKVRV SYQKLLKYYV LNALKHRPPK AQKKRYLFRS FKATK FFQS TKLDWVEVGL QVCRQGYNML NLLIHRKNLN YLHLDYNFNL KPVKTLTTKE RKKSRFGNAF HLCREVLRLT KLVVDS HVQ YRLGNVDAFQ LADGLQYIFA HVGQLTGMYR YKYKLMRQIR MCKDLKHLIY YRFNTGPVGK GPGCGFWAAG WRVWLFF MR GITPLLERWL GNLLARQFEG RHSKGVAKTV TKQRVESHFD LELRAAVMHD ILDMMPEGIK QNKARTILQH LSEAWRCW K ANIPWKVPGL PTPIENMILR YVKAKADWWT NTAHYNRERI RRGATVDKTV CKKNLGRLTR LYLKAEQERQ HNYLKDGPY ITAEEAVAVY TTTVHWLESR RFSPIPFPPL SYKHDTKLLI LALERLKEAY SVKSRLNQSQ REELGLIEQA YDNPHEALSR IKRHLLTQR AFKEVGIEFM DLYSHLVPVY DVEPLEKITD AYLDQYLWYE ADKRRLFPPW IKPADTEPPP LLVYKWCQGI N NLQDVWET SEGECNVMLE SRFEKMYEKI DLTLLNRLLR LIVDHNIADY MTAKNNVVIN YKDMNHTNSY GIIRGLQFAS FI VQYYGLV MDLLVLGLHR ASEMAGPPQM PNDFLSFQDI ATEAAHPIRL FCRYIDRIHI FFRFTADEAR DLIQRYLTEH PDP NNENIV GYNNKKCWPR DARMRLMKHD VNLGRAVFWD IKNRLPRSVT TVQWENSFVS VYSKDNPNLL FNMCGFECRI LPKC RTSYE EFTHKDGVWN LQNEVTKERT AQCFLRVDDE SMQRFHNRVR QILMASGSTT FTKIVNKWNT ALIGLMTYFR EAVVN TQEL LDLLVKCENK IQTRIKIGLN SKMPSRFPPV VFYTPKELGG LGMLSMGHVL IPQSDLRWSK QTDVGITHFR SGMSHE EDQ LIPNLYRYIQ PWESEFIDSQ RVWAEYALKR QEAIAQNRRL TLEDLEDSWD RGIPRINTLF QKDRHTLAYD KGWRVRT DF KQYQVLKQNP FWWTHQRHDG KLWNLNNYRT DMIQALGGVE GILEHTLFKG TYFPTWEGLF WEKASGFEES MKWKKLTN A QRSGLNQIPN RRFTLWWSPT INRANVYVGF QVQLDLTGIF MHGKIPTLKI SLIQIFRAHL WQKIHESIVM DLCQVFDQE LDALEIETVQ KETIHPRKSY KMNSSCADIL LFASYKWNVS RPSLLADSKD VMDSTTTQKY WIDIQLRWGD YDSHDIERYA RAKFLDYTT DNMSIYPSPT GVLIAIDLAY NLHSAYGNWF PGSKPLIQQA MAKIMKANPA LYVLRERIRK GLQLYSSEPT E PYLSSQNY GELFSNQIIW FVDDTNVYRV TIHKTFEGNL TTKPINGAIF IFNPRTGQLF LKIIHTSVWA GQKRLGQLAK WK TAEEVAA LIRSLPVEEQ PKQIIVTRKG MLDPLEVHLL DFPNIVIKGS ELQLPFQACL KVEKFGDLIL KATEPQMVLF NLY DDWLKT ISSYTAFSRL ILILRALHVN NDRAKVILKP DKTTITEPHH IWPTLTDEEW IKVEVQLKDL ILADYGKKNN VNVA SLTQS EIRDIILGME ISAPSQQRQQ IAEIEKQTKE QSQLTATQTR TVNKHGDEII TSTTSNYETQ TFSSKTEWRV RAISA ANLH LRTNHIYVSS DDIKETGYTY ILPKNVLKKF ICISDLRAQI AGYLYGVSPP DNPQVKEIRC IVMVPQWGTH QTVHLP GQL PQHEYLKEME PLGWIHTQPN ESPQLSPQDV TTHAKIMADN PSWDGEKTII ITCSFTPGSC TLTAYKLTPS GYEWGRQ NT DKGNNPKGYL PSHYERVQML LSDRFLGFFM VPAQSSWNYN FMGVRHDPNM KYELQLANPK EFYHEVHRPS HFLNFALL Q EGEVYSADRE DLYA

Macromolecule #8: U5 small nuclear ribonucleoprotein 200 kDa helicase

• Macromolecule: Name: U5 small nuclear ribonucleoprotein 200 kDa helicase / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 197.053984 KDa

Sequence

String:

APRQVLDLED LVFTQGSHFM ANKRCQLPDG SFRRQRKGYE EVHVPALKPK PFGSEEQLLP VEKLPKYAQA GFEGFKTLNR IQSKLYRAA LETDENLLLC APTGAGKTNV ALMCMLREIG KHINMDGTIN VDDFKIIYIA PMRSLVQEMV GSFGKRLATY G ITVAELTG DHQLCKEEIS ATQIIVCTPE KWDIITRKGG ERTYTQLVRL IILDEIHLLH DDRGPVLEAL VARAIRNIEM TQ EDVRLIG LSATLPNYED VATFLRVDPA KGLFYFDNSF RPVPLEQTYV GITEKKAIKR FQIMNEIVYE KIMEHAGKNQ VLV FVHSRK ETGKTARAIR DMCLEKDTLG LFLREGSAST EVLRTEAEQC KNLELKDLLP YGFAIHHAGM TRVDRTLVED LFAD KHIQV LVSTATLAWG VNLPAHTVII KGTQVYSPEK GRWTELGALD ILQMLGRAGR PQYDTKGEGI LITSHGELQY YLSLL NQQL PIESQMVSKL PDMLNAEIVL GNVQNAKDAV NWLGYAYLYI RMLRSPTLYG ISHDDLKGDP LLDQRRLDLV HTAALM LDK NNLVKYDKKT GNFQVTELGR IASHYYITND TVQTYNQLLK PTLSEIELFR VFSLSSEFKN ITVREEEKLE LQKLLER VP IPVKESIEEP SAKINVLLQA FISQLKLEGF ALMADMVYVT QSAGRLMRAI FEIVLNRGWA QLTDKTLNLC KMIDKRMW Q SMCPLRQFRK LPEEVVKKIE KKNFPFERLY DLNHNEIGEL IRMPKMGKTI HKYVHLFPKL ELSVHLQPIT RSTLKVELT ITPDFQWDEK VHGSSEAFWI LVEDVDSEVI LHHEYFLLKA KYAQDEHLIT FFVPVFEPLP PQYFIRVVSD RWLSCETQLP VSFRHLILP EKYPPPTELL DLQPLPVSAL RNSAFESLYQ DKFPFFNPIQ TQVFNTVYNS DDNVFVGAPT GSGKTICAEF A ILRMLLQS SEGRCVYITP MEALAEQVYM DWYEKFQDRL NKKVVLLTGE TSTDLKLLGK GNIIISTPEK WDILSRRWKQ RK NVQNINL FVVDEVHLIG GENGPVLEVI CSRMRYISSQ IERPIRIVAL SSSLSNAKDV AHWLGCSATS TFNFHPNVRP VPL ELHIQG FNISHTQTRL LSMAKPVYHA ITKHSPKKPV IVFVPSRKQT RLTAIDILTT CAADIQRQRF LHCTEKDLIP YLEK LSDST LKETLLNGVG YLHEGLSPME RRLVEQLFSS GAIQVVVASR SLCWGMNVAA HLVIIMDTQY YNGKIHAYVD YPIYD VLQM VGHANRPLQD DEGRCVIMCQ GSKKDFFKKF LYEPLPVESH LDHCMHDHFN AEIVTKTIEN KQDAVDYLTW TFLYRR MTQ NPNYYNLQGI SHRHLSDHLS ELVEQTLSDL EQSKCISIED EMDVAPLNLG MIAAYYYINY TTIELFSMSL NAKTKVR GL IEIISNAAEY ENIPIRHHED NLLRQLAQKV PHKLNNPKFN DPHVKTNLLL QAHLSRMQLS AELQSDTEEI LSKAIRLI Q ACVDVLSSNG WLSPALAAME LAQMVTQAMW SKDSYLKQLP HFTSEHIKRC TDKGVESVFD IMEMEDEERN ALLQLTDSQ IADVARFCNR YPNIELSYEV VDKDSIRSGG PVVVLVQLER EEEVTGPVIA PLFPQKREEG WWVVIGDAKS NSLISIKRLT LQQKAKVKL DFVAPATGAH NYTLYFMSDA YMGCDQEYKF SVD

Macromolecule #9: 116 kDa U5 small nuclear ribonucleoprotein component

• Macromolecule: Name: 116 kDa U5 small nuclear ribonucleoprotein component / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 101.231938 KDa

Sequence

String:

EVVLHEDKKY YPTAEEVYGP EVETIVQEED TQPLTEPIIK PVKTKKFTLM EQTLPVTVYE MDFLADLMDN SELIRNVTLC GHLHHGKTC FVDCLIEQTH PEIRKRYDQD LCYTDILFTE QERGVGIKST PVTVVLPDTK GKSYLFNIMD TPGHVNFSDE V TAGLRISD GVVLFIDAAE GVMLNTERLI KHAVQERLAV TVCINKIDRL ILELKLPPTD AYYKLRHIVD EVNGLISMYS TD ENLILSP LLGNVCFSSS QYSICFTLGS FAKIYADTFG DINYQEFAKR LWGDIYFNPK TRKFTKKAPT SSSQRSFVEF ILE PLYKIL AQVVGDVDTS LPRTLDELGI HLTKEELKLN IRPLLRLVCK KFFGEFTGFV DMCVQHIPSP KVGAKPKIEH TYTG GVDSD LGEAMSDCDP DGPLMCHTTK MYSTDDGVQF HAFGRVLSGT IHAGQPVKVL GENYTLEDEE DSQICTVGRL WISVA RYHI EVNRVPAGNW VLIEGVDQPI VKTATITEPR GNEEAQIFRP LKFNTTSVIK IAVEPVNPSE LPKMLDGLRK VNKSYP SLT TKVEESGEHV ILGTGELYLD CVMHDLRKMY SEIDIKVADP VVTFCETVVE TSSLKCFAET PNKKNKITMI AEPLEKG LA EDIENEVVQI TWNRKKLGEF FQTKYDWDLL AARSIWAFGP DATGPNILVD DTLPSEVDKA LLGSVKDSIV QGFQWGTR E GPLCDELIRN VKFKILDAVV AQEPLHRGGG QIIPTARRVV YSAFLMATPR LMEPYYFVEV QAPADCVSAV YTVLARRRG HVTQDAPIPG SPLYTIKAFI PAIDSFGFET DLRTHTQGQA FSLSVFHHWQ IVPGDPLDKS IVIRPLEPQP APHLAREFMI KTRRRKGLS EDVSIKFFDD

Macromolecule #10: PRKR-interacting protein 1

• Macromolecule: Name: PRKR-interacting protein 1 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 14.476882 KDa

Sequence

String:

TLVIPKNAAE EQKLKLERLM KNPDKAVPIP EKMSEWAPRP PPEFVRDVMG SSAGAGSGEF HVYRHLRRRE YQRQDYMDAM AEKQKLDAE FQKRLEKNKI AAEEQTAKRR KKRQKLKEKK LLAK

Macromolecule #12: Cactin

• Macromolecule: Name: Cactin / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 15.210249 KDa

Sequence

String:

KYRPRKPRFF NRVHTGFAWN KYNQTHYDFD NPPPKIVQGY KFNIFYPDLI DKRSTPEYFL EACADNKDFA ILRFHAGPPY EDIAFKIVN REWEYSHRHG FRCQFANGIF QLWFHFKRYR YRR

Macromolecule #13: Protein FAM32A

• Macromolecule: Name: Protein FAM32A / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 7.269374 KDa

Sequence

String:

RGLDKRTPAQ AAFEKMQEKR QMERILKKAS KTHKQRVEDF NRHLDTLTEH YDIPKVSWTK

Macromolecule #14: Pre-mRNA-splicing factor CWC22 homolog

• Macromolecule: Name: Pre-mRNA-splicing factor CWC22 homolog / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 105.646578 KDa

Sequence

String:

MKSSVAQIKP SSGHDRRENL NSYQRNSSPE DRYEEQERSP RDRDYFDYSR SDYEHSRRGR SYDSSMESRN RDREKRRERE RDTDRKRSR KSPSPGRRNP ETSVTQSSSA QDEPATKKKK DELDPLLTRT GGAYIPPAKL RMMQEQITDK NSLAYQRMSW E ALKKSING LINKVNISNI SIIIQELLQE NIVRGRGLLS RSVLQAQSAS PIFTHVYAAL VAIINSKFPQ IGELILKRLI LN FRKGYRR NDKQLCLTAS KFVAHLINQN VAHEVLCLEM LTLLLERPTD DSVEVAIGFL KECGLKLTQV SPRGINAIFE RLR NILHES EIDKRVQYMI EVMFAVRKDG FKDHPIILEG LDLVEEDDQF THMLPLEDDY NPEDVLNVFK MDPNFMENEE KYKA IKKEI LDEGDTDSNT DQDAGSSEED EEEEEEEGEE DEEGQKVTIH DKTEINLVSF RRTIYLAIQS SLDFEECAHK LLKME FPES QTKELCNMIL DCCAQQRTYE KFFGLLAGRF CMLKKEYMES FEGIFKEQYD TIHRLETNKL RNVAKMFAHL LYTDSL PWS VLECIKLSEE TTTSSSRIFV KIFFQELCEY MGLPKLNARL KDETLQPFFE GLLPRDNPRN TRFAINFFTS IGLGGLT DE LREHLKNTPK VIVAQKPDVE QNKSSPSSSS SASSSSESDS SDSDSDSSDS SSESSSEESD SSSISSHSSA SANDVRKK G HGKTRSKEVD KLIRNQQTND RKQKERRQEH GHQETRTERE RRSEKHRDQN SSGSNWRDPI TKYTSDKDVP SERNNYSRV ANDRDQEMHI DLENKHGDPK KKRGERRNSF SENEKHTHRI KDSENFRRKD RSKSKEMNRK HSGSRSDEDR YQNGAERRWE KSSRYSEQS RESKKNQDRR REKSPAKQK

Macromolecule #16: Pleiotropic regulator 1

• Macromolecule: Name: Pleiotropic regulator 1 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 35.782395 KDa

Sequence

String:

MPKPQWHPPW KLYRVISGHL GWVRCIAVEP GNQWFVTGSA DRTIKIWDLA SGKLKLSLTG HISTVRGVIV STRSPYLFSC GEDKQVKCW DLEYNKVIRH YHGHLSAVYG LDLHPTIDVL VTCSRDSTAR IWDVRTKASV HTLSGHTNAV ATVRCQAAEP Q IITGSHDT TIRLWDLVAG KTRVTLTNHK KSVRAVVLHP RHYTFASGSP DNIKQWKFPD GSFIQNLSGH NAIINTLTVN SD GVLVSGA DNGTMHLWDW RTGYNFQRVH AAVQPGSLDS ESGIFACAFD QSESRLLTAE ADKTIKVYRE DDTATEETHP VSW

Macromolecule #17: SNW domain-containing protein 1

• Macromolecule: Name: SNW domain-containing protein 1 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 33.819922 KDa

Sequence

String:

PPPYGYRKGW IPRLLEDFGD GGAFPEIHVA QYPLDMGRKK KMSNALAIQV DSEGKIKYDA IARQGQSKDK VIYSKYTDLV PKEVMNADD PDLQRPDEEA IKEITEKTRV ALEKSVSQKV AAAMPVRAAD KLAPAQYIRY TPSQQGVAFN SGAKQRVIRM V EMQKDPME PPRFKINKKI PRGPP(SEP)PPAP VMH(SEP)PSRKMT VKEQQEWKIP PCISNWKNAK GYTIPLDKRL AADG RGLQT VHINENFAKL AEALYIADRK AREAVEMRAQ VERKMAQKEK EKHEEKLREM AQKARERR

Macromolecule #18: Protein BUD31 homolog

• Macromolecule: Name: Protein BUD31 homolog / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 17.03285 KDa

Sequence

String:

MPKVKRSRKA PPDGWELIEP TLDELDQKMR EAETEPHEGK RKVESLWPIF RIHHQKTRYI FDLFYKRKAI SRELYEYCIK EGYADKNLI AKWKKQGYEN LCCLRCIQTR DTNFGTNCIC RVPKSKLEVG RIIECTHCGC RGCSG

Macromolecule #19: Pre-mRNA-splicing factor RBM22

• Macromolecule: Name: Pre-mRNA-splicing factor RBM22 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 33.156129 KDa

Sequence

String:

ADFPILCQTC LGENPYIRMT KEKYGKECKI CARPFTVFRW CPGVRMRFKK TEVCQTCSKL KNVCQTCLLD LEYGLPIQVR DAGLSFKDD MPKSDVNKEY YTQNMEREIS NSDGTRPVGM LGKATSAAAA LLKLARTTPY YKRNRPHICS FWVKGECKRG E ECPYRHEK PTDPDDPLAD QNIKDRYYGI NDPVADKLLK RASTMPRLDP PEDKTITTLY VGGLGDTITE TDLRNHFYQF GE IRTITVV QRQQCAFIQF ATRQAAEVAA EKSFNKLIVN GRRLNVKWGR SQ

Macromolecule #20: U5 small nuclear ribonucleoprotein 40 kDa protein

• Macromolecule: Name: U5 small nuclear ribonucleoprotein 40 kDa protein / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 34.09241 KDa

Sequence

String:

RCSSLQPIML LSGHEGEVYC CKFHPNGSTL ASAGFDRLIL LWNVYGDCDN YATLKGHSGA VMELHYNTDG SMLFSASTDK TVAVWDSET GERVKRLKGH TSFVNSCYPA RRGPQLVCTG SDDGTVKLWD IRKKAAIQTF QNTYQVLAVT FNDTSDQIIS G GIDNDIKV WDLRQNKLTY TMRGHADSVT GLSLSSEGSY LLSNAMDNTV RVWDVRPFAP KERCVKIFQG NVHNFEKNLL RC SWSPDGS KIAAGSADRF VYVWDTTSRR ILYKLPGHAG SINEVAFHPD EPIIISASSD KRLYMGEIQ

Macromolecule #21: Cell division cycle 5-like protein

• Macromolecule: Name: Cell division cycle 5-like protein / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 92.406883 KDa

Sequence

String:

MPRIMIKGGV WRNTEDEILK AAVMKYGKNQ WSRIASLLHR KSAKQCKARW YEWLDPSIKK TEWSREEEEK LLHLAKLMPT QWRTIAPII GRTAAQCLEH YEFLLDKAAQ RDNEEETTDD PRKLKPGEID PNPETKPARP DPIDMDEDEL EMLSEARARL A NTQGKKAK RKAREKQLEE ARRLAALQKR RELRAAGIEI QKKRKRKRGV DYNAEIPFEK KPALGFYDTS EENYQALDAD FR KLRQQDL DGELRSEKEG RDRKKDKQHL KRKKESDLPS AILQTSGVSE FTKKRSKLVL PAPQISDAEL QEVVKVGQAS EIA RQTAEE SGITNSASST LLSEYNVTNN SVALRTPRTP ASQDRILQEA QNLMALTNVD TPLKGGLNTP LHESDFSGVT PQRQ VVQTP NTVLSTPFRT PSNGAEGLTP RSGTTPKPVI NSTPGRTPLR DKLNINPEDG MADYSDPSYV KQMERESREH LRLGL LGLP APKNDFEIVL PENAEKELEE REIDDTYIED AADVDARKQA IRDAERVKEM KRMHKAVQKD LPRPSEVNET ILRPLN VEP PLTDLQKSEE LIKKEMITML HYDLLHHPYE PSGNKKGKTV GFGTNNSEHI TYLEHNPYEK FSKEELKKAQ DVLVQEM EV VKQGMSHGEL SSEAYNQVWE ECYSQVLYLP GQSRYTRANL ASKKDRIESL EKRLEINRGH MTTEAKRAAK MEKKMKIL L GGYQSRAMGL MKQLNDLWDQ IEQAHLELRT FEELKKHEDS AIPRRLECLK EDVQRQQERE KELQHRYADL LLEKETLKS KF

Macromolecule #22: Spliceosome-associated protein CWC15 homolog

• Macromolecule: Name: Spliceosome-associated protein CWC15 homolog / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 26.674447 KDa

Sequence

String:

MTTAARPTFE PARGGRGKGE GDLSQLSKQY SSRDLPSHTK IKYRQTTQDA PEEVRNRDFR RELEERERAA AREKNRDRPT REHTTSSSV SKKPRLDQIP AANLDADDPL TDEEDEDFEE ESDDDDTAAL LAELEKIKKE RAEEQARKEQ EQKAEEERIR M ENILSGNP LLNLTGPSQP QANFKVKRRW DDDVVFKNCA KGVDDQKKDK RFVNDTLRSE FHKKFMEKYI K

Macromolecule #23: Serine/arginine repetitive matrix protein 2

• Macromolecule: Name: Serine/arginine repetitive matrix protein 2 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 2.767128 KDa

Sequence

String:

MYNGIGLPTP RGSGTNGYVQ RNLSLV

Macromolecule #24: Crooked neck-like protein 1

• Macromolecule: Name: Crooked neck-like protein 1 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 100.610008 KDa

Sequence

String:

MTATVENLTF QKDTLGNAVD KNTSRLELRS YSLAGRHGST EPLVLAWSSQ FRRLTWGCAL DALHRSPCVA ASQHGVTHLI RSSRTPHST RCRKEDAQPG HHGNGAASVT AQARGQRSVL QVPLPVPRSC LFSESFVVSV SSQSRFLASV PGTGVQRSTA A DMAASTAA GKQRIPKVAK VKNKAPAEVQ ITAEQLLREA KERELELLPP PPQQKITDEE ELNDYKLRKR KTFEDNIRKN RT VISNWIK YAQWEESLKE IQRARSIYER ALDVDYRNIT LWLKYAEMEM KNRQVNHARN IWDRAITTLP RVNQFWYKYT YME EMLGNV AGARQVFERW MEWQPEEQAW HSYINFELRY KEVDRARTIY ERFVLVHPDV KNWIKYARFE EKHAYFAHAR KVYE RAVEF FGDEHMDEHL YVAFAKFEEN QKEFERVRVI YKYALDRISK QDAQELFKNY TIFEKKFGDR RGIEDIIVSK RRFQY EEEV KANPHNYDAW FDYLRLVESD AEAEAVREVY ERAIANVPPI QEKRHWKRYI YLWINYALYE ELEAKDPERT RQVYQA SLE LIPHKKFTFA KMWILYAQFE IRQKNLSLAR RALGTSIGKC PKNKLFKVYI ELELQLREFD RCRKLYEKFL EFGPENC TS WIKFAELETI LGDIDRARAI YELAISQPRL DMPEVLWKSY IDFEIEQEET ERTRNLYRRL LQRTQHVKVW ISFAQFEL S SGKEGSLTKC RQIYEEANKT MRNCEEKEER LMLLESWRSF EEEFGTASDK ERVDKLMPEK VKKRRKVQTD DGSDAGWEE YFDYIFPEDA ANQPNLKLLA MAKLWKKQQQ EKEDAEHHPD EDVDESES

Macromolecule #25: Pre-mRNA-splicing factor SYF1

• Macromolecule: Name: Pre-mRNA-splicing factor SYF1 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 100.148711 KDa

Sequence

String:

MVVMARLSRP ERPDLVFEEE DLPYEEEIMR NQFSVKCWLR YIEFKQGAPK PRLNQLYERA LKLLPCSYKL WYRYLKARRA QVKHRCVTD PAYEDVNNCH ERAFVFMHKM PRLWLDYCQF LMDQGRVTHT RRTFDRALRA LPITQHSRIW PLYLRFLRSH P LPETAVRG YRRFLKLSPE SAEEYIEYLK SSDRLDEAAQ RLATVVNDER FVSKAGKSNY QLWHELCDLI SQNPDKVQSL NV DAIIRGG LTRFTDQLGK LWCSLADYYI RSGHFEKARD VYEEAIRTVM TVRDFTQVFD SYAQFEESMI AAKMETASEL GRE EEDDVD LELRLARFEQ LISRRPLLLN SVLLRQNPHH VHEWHKRVAL HQGRPREIIN TYTEAVQTVD PFKATGKPHT LWVA FAKFY EDNGQLDDAR VILEKATKVN FKQVDDLASV WCQCGELELR HENYDEALRL LRKATALPAR RAEYFDGSEP VQNRV YKSL KVWSMLADLE ESLGTFQSTK AVYDRILDLR IATPQIVINY AMFLEEHKYF EESFKAYERG ISLFKWPNVS DIWSTY LTK FIARYGGRKL ERARDLFEQA LDGCPPKYAK TLYLLYAQLE EEWGLARHAM AVYERATRAV EPAQQYDMFN IYIKRAA EI YGVTHTRGIY QKAIEVLSDE HAREMCLRFA DMECKLGEID RARAIYSFCS QICDPRTTGA FWQTWKDFEV RHGNEDTI K EMLRIRRSVQ ATYNTQVNFM ASQMLKVSGS ATGTVSDLAP GQSGMDDMKL LEQRAEQLAA EAERDQPLRA QSKILFVRS DASREELAEL AQQVNPEEIQ LGEDEDEDEM DLEPNEVRLE QQSVPAAVFG SLKED

Macromolecule #26: Intron-binding protein aquarius

• Macromolecule: Name: Intron-binding protein aquarius / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 171.502453 KDa

Sequence

String:

MAAPAQPKKI VAPTVSQINA EFVTQLACKY WAPHIKKKSP FDIKVIEDIY EKEIVKSRFA IRKIMLLEFS QYLENYLWMN YSPEVSSKA YLMSICCMVN EKFRENVPAW EIFKKKPDHF PFFFKHILKA ALAETDGEFS LHEQTVLLLF LDHCFNSLEV D LIRSQVQQ LISLPMWMGL QLARLELELK KTPKLRKFWN LIKKNDEKMD PEAREQAYQE RRFLSQLIQK FISVLKSVPL SE PVTMDKV HYCERFIELM IDLEALLPTR RWFNTILDDS HLLVHCYLSN LVRREEDGHL FSQLLDMLKF YTGFEINDQT GNA LTENEM TTIHYDRITS LQRAAFAHFP ELYDFALSNV AEVDTRESLV KFFGPLSSNT LHQVASYLCL LPTLPKNEDT TFDK EFLLE LLVSRHERRI SQIQQLNQMP LYPTEKIIWD ENIVPTEYYS GEGCLALPKL NLQFLTLHDY LLRNFNLFRL ESTYE IRQD IEDSVSRMKP WQSEYGGVVF GGWARMAQPI VAFTVVEVAK PNIGENWPTR VRADVTINLN VRDHIKDEWE GLRKHD VCF LITVRPTKPY GTKFDRRRPF IEQVGLVYVR GCEIQGMLDD KGRVIEDGPE PRPNLRGESR TFRVFLDPNQ YQQDMTN TI QNGAEDVYET FNIIMRRKPK ENNFKAVLET IRNLMNTDCV VPDWLHDIIL GYGDPSSAHY SKMPNQIATL DFNDTFLS I EHLKASFPGH NVKVTVEDPA LQIPPFRITF PVRSGKGKKR KDADVEDEDT EEAKTLIVEP HVIPNRGPYP YNQPKRNTI QFTHTQIEAI RAGMQPGLTM VVGPPGTGKT DVAVQIISNI YHNFPEQRTL IVTHSNQALN QLFEKIMALD IDERHLLRLG HGEEELETE KDFSRYGRVN YVLARRIELL EEVKRLQKSL GVPGDASYTC ETAGYFFLYQ VMSRWEEYIS KVKNKGSTLP D VTEVSTFF PFHEYFANAP QPIFKGRSYE EDMEIAEGCF RHIKKIFTQL EEFRASELLR SGLDRSKYLL VKEAKIIAMT CT HAALKRH DLVKLGFKYD NILMEEAAQI LEIETFIPLL LQNPQDGFSR LKRWIMIGDH HQLPPVIKNM AFQKYSNMEQ SLF TRFVRV GVPTVDLDAQ GRARASLCNL YNWRYKNLGN LPHVQLLPEF STANAGLLYD FQLINVEDFQ GVGESEPNPY FYQN LGEAE YVVALFMYMC LLGYPADKIS ILTTYNGQKH LIRDIINRRC GNNPLIGRPN KVTTVDRFQG QQNDYILLSL VRTRA VGHL RDVRRLVVAM SRARLGLYIF ARVSLFQNCF ELTPAFSQLT ARPLHLHIIP TEPFPTTRKN GERPSHEVQI IKNMPQ MAN FVYNMYMHLI QTTHHYHQTL LQLPPAMVEE GEEVQNQETE LETEEEAMTV QADIIPSPTD TSCRQETPAF QTDTTPS ET GATSTPEAIP ALSETTPTVV GAVSAPAEAN TPQDATSAPE ETK

Macromolecule #27: ATP-dependent RNA helicase DHX8

• Macromolecule: Name: ATP-dependent RNA helicase DHX8 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 139.522312 KDa
• Recombinant expression: Organism: Saccharomyces cerevisiae (brewer's yeast)

Sequence

String:

MAVAVAMAGA LIGSEPGPAE ELAKLEYLSL VSKVCTELDN HLGINDKDLA EFVISLAEKN TTFDTFKASL VKNGAEFTDS LISNLLRLI QTMRPPAKPS TSKDPVVKPK TEKEKLKELF PVLCQPDNPS VRTMLDEDDV KVAVDVLKEL EALMPSAAGQ E KQRDAEHR DRTKKKKRSR SRDRNRDRDR DRERNRDRDH KRRHRSRSRS RSRTRERNKV KSRYRSRSRS QSPPKDRKDR DK YGERNLD RWRDKHVDRP PPEEPTIGDI YNGKVTSIMQ FGCFVQLEGL RKRWEGLVHI SELRREGRVA NVADVVSKGQ RVK VKVLSF TGTKTSLSMK DVDQETGEDL NPNRRRNLVG ETNEETSMRN PDRPTHLSLV SAPEVEDDSL ERKRLTRISD PEKW EIKQM IAANVLSKEE FPDFDEETGI LPKVDDEEDE DLEIELVEEE PPFLRGHTKQ SMDMSPIKIV KNPDGSLSQA AMMQS ALAK ERRELKQAQR EAEMDSIPMG LNKHWVDPLP DAEGRQIAAN MRGIGMMPND IPEWKKHAFG GNKASYGKKT QMSILE QRE SLPIYKLKEQ LVQAVHDNQI LIVIGETGSG KTTQITQYLA EAGYTSRGKI GCTQPRRVAA MSVAKRVSEE FGCCLGQ EV GYTIRFEDCT SPETVIKYMT DGMLLRECLI DPDLTQYAII MLDEAHERTI HTDVLFGLLK KTVQKRQDMK LIVTSATL D AVKFSQYFYE APIFTIPGRT YPVEILYTKE PETDYLDASL ITVMQIHLTE PPGDILVFLT GQEEIDTACE ILYERMKSL GPDVPELIIL PVYSALPSEM QTRIFDPAPP GSRKVVIATN IAETSLTIDG IYYVVDPGFV KQKVYNSKTG IDQLVVTPIS QAQAKQRAG RAGRTGPGKC YRLYTERAYR DEMLTTNVPE IQRTNLASTV LSLKAMGIND LLSFDFMDAP PMETLITAME Q LYTLGALD DEGLLTRLGR RMAEFPLEPM LCKMLIMSVH LGCSEEMLTI VSMLSVQNVF YRPKDKQALA DQKKAKFHQT EG DHLTLLA VYNSWKNNKF SNPWCYENFI QARSLRRAQD IRKQMLGIMD RHKLDVVSCG KSTVRVQKAI CSGFFRNAAK KDP QEGYRT LIDQQVVYIH PSSALFNRQP EWVVYHELVL TTKEYMREVT TIDPRWLVEF APAFFKVSDP TKLSKQKKQQ RLEP LYNRY EEPNAWRISR AFRRR

Macromolecule #28: U2 small nuclear ribonucleoprotein A'

• Macromolecule: Name: U2 small nuclear ribonucleoprotein A' / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 18.69773 KDa

Sequence

String:

VKLTAELIEQ AAQYTNAVRD RELDLRGYKI PVIENLGATL DQFDAIDFSD NEIRKLDGFP LLRRLKTLLV NNNRICRIGE GLDQALPCL TELILTNNSL VELGDLDPLA SLKSLTYLSI LRNPVTNKKH YRLYVIYKVP QVRVLDFQKV KLKERQEAEK M FK

Macromolecule #29: U2 small nuclear ribonucleoprotein B''

• Macromolecule: Name: U2 small nuclear ribonucleoprotein B'' / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 10.688563 KDa

Sequence

String:

IRPNHTIYIN NMNDKIKKEE LKRSLYALFS QFGHVVDIVA LKTMKMRGQA FVIFKELGSS TNALRQLQGF PFYGKPMRIQ YAKTDSDII SKM

Macromolecule #30: NF-kappa-B-activating protein

• Macromolecule: Name: NF-kappa-B-activating protein / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 3.321766 KDa

Sequence

String:

KRIPRRGEIG LTSEEIASFE CSGYVMSGSR

Macromolecule #31: Small nuclear ribonucleoprotein-associated proteins B and B'

• Macromolecule: Name: Small nuclear ribonucleoprotein-associated proteins B and B'; type: protein_or_peptide / ID: 31 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 9.551284 KDa

Sequence

String:

SSKMLQHIDY RMRCILQDGR IFIGTFKAFD KHMNLILCDC DEFRKIKPKN SKQAEREEKR VLGLVLLRGE NLVSMTVEGP PP

Macromolecule #32: Pre-mRNA-splicing factor SLU7

• Macromolecule: Name: Pre-mRNA-splicing factor SLU7 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 68.510844 KDa

Sequence

String:

MSATVVDAVN AAPLSGSKEM SLEEPKKMTR EDWRKKKELE EQRKLGNAPA EVDEEGKDIN PHIPQYISSV PWYIDPSKRP TLKHQRPQP EKQKQFSSSG EWYKRGVKEN SIITKYRKGA CENCGAMTHK KKDCFERPRR VGAKFTGTNI APDEHVQPQL M FDYDGKRD RWNGYNPEEH MKIVEEYAKV DLAKRTLKAQ KLQEELASGK LVEQANSPKH QWGEEEPNSQ MEKDHNSEDE DE DKYADDI DMPGQNFDSK RRITVRNLRI REDIAKYLRN LDPNSAYYDP KTRAMRENPY ANAGKNPDEV SYAGDNFVRY TGD TISMAQ TQLFAWEAYD KGSEVHLQAD PTKLELLYKS FKVKKEDFKE QQKESILEKY GGQEHLDAPP AELLLAQTED YVEY SRHGT VIKGQERAVA CSKYEEDVKI HNHTHIWGSY WKEGRWGYKC CHSFFKYSYC TGEAGKEIVN SEECIINEIT GEESV KKPQ TLMELHQEKL KEEKKKKKKK KKKHRKSSSD SDDEEKKHEK LKKALNAEEA RLLHVKETMQ IDERKRPYNS MYETRE PTE EEMEAYRMKR QRPDDPMASF LGQ

Macromolecule #33: Small nuclear ribonucleoprotein Sm D3

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D3 / type: protein_or_peptide / ID: 33 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 9.460979 KDa

Sequence

String:

SIGVPIKVLH EAEGHIVTCE TNTGEVYRGK LIEAEDNMNC QMSNITVTYR DGRVAQLEQV YIRGSKIRFL ILPDMLKNAP MLKS

Macromolecule #34: Small nuclear ribonucleoprotein E

• Macromolecule: Name: Small nuclear ribonucleoprotein E / type: protein_or_peptide / ID: 34 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 9.582185 KDa

Sequence

String:

QKVMVQPINL IFRYLQNRSR IQVWLYEQVN MRIEGCIIGF DEYMNLVLDD AEEIHSKTKS RKQLGRIMLK GDNITLLQSV S

Macromolecule #35: Small nuclear ribonucleoprotein F

• Macromolecule: Name: Small nuclear ribonucleoprotein F / type: protein_or_peptide / ID: 35 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 8.038415 KDa

Sequence

String:

PLNPKPFLNG LTGKPVMVKL KWGMEYKGYL VSVDGYMNMQ LANTEEYIDG ALSGHLGEVL IRCNNVLYIR GV

Macromolecule #36: Small nuclear ribonucleoprotein G

• Macromolecule: Name: Small nuclear ribonucleoprotein G / type: protein_or_peptide / ID: 36 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 8.160629 KDa

Sequence

String:

AHPPELKKFM DKKLSLKLNG GRHVQGILRG FDPFMNLVID ECVEMATSGQ QNNIGMVVIR GNSIIMLEAL ERV

Macromolecule #37: Small nuclear ribonucleoprotein Sm D1

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D1 / type: protein_or_peptide / ID: 37 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 9.086731 KDa

Sequence

String:

MKLVRFLMKL SHETVTIELK NGTQVHGTIT GVDVSMNTHL KAVKMTLKNR EPVQLETLSI RGNNIRYFIL PDSLPLDTLL

Macromolecule #38: Peptidyl-prolyl cis-trans isomerase-like 1

• Macromolecule: Name: Peptidyl-prolyl cis-trans isomerase-like 1 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO / EC number: peptidylprolyl isomerase
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 18.055529 KDa

Sequence

String:

AIPPDSWQPP NVYLETSMGI IVLELYWKHA PKTCKNFAEL ARRGYYNGTK FHRIIKDFMI QGGDPTGTGR GGASIYGKQF EDELHPDLK FTGAGILAMA NAGPDTNGSQ FFVTLAPTQW LDGKHTIFGR VCQGIGMVNR VGMVETNSQD RPVDDVKIIK A YPSG

Macromolecule #39: Small nuclear ribonucleoprotein Sm D2

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D2 / type: protein_or_peptide / ID: 39 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 13.551928 KDa

Sequence

String:

MSLLNKPKSE MTPEELQKRE EEEFNTGPLS VLTQSVKNNT QVLINCRNNK KLLGRVKAFD RHCNMVLENV KEMWTEVPKS GKGKKKSKP VNKDRYISKM FLRGDSVIVV LRNPLIAGK

Macromolecule #40: Pre-mRNA-processing factor 17

• Macromolecule: Name: Pre-mRNA-processing factor 17 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 59.116055 KDa

Sequence

String:

ETGVHLDPAV KEVQYNPTYE TMFAPEFGPE NPFRTQQMAA PRNMLSGYAE PAHINDFMFE QQRRTFATYG YALDPSLDNH QVSAKYIGS VEEAEKNQGL TVFETGQKKT EKRKKFKEND ASNIDGFLGP WAKYVDEKDV AKPSEEEQKE LDEITAKRQK K GKQEEEKP GEEKTILHVK EMYDYQGRSY LHIPQDVGVN LRSTMPPEKC YLPKKQIHVW SGHTKGVSAV RLFPLSGHLL LS CSMDCKI KLWEVYGERR CLRTFIGHSK AVRDICFNTA GTQFLSAAYD RYLKLWDTET GQCISRFTNR KVPYCVKFNP DED KQNLFV AGMSDKKIVQ WDIRSGEIVQ EYDRHLGAVN TIVFVDENRR FVSTSDDKSL RVWEWDIPVD FKYIAEPSMH SMPA VTLSP NGKWLACQSM DNQILIFGAQ NRFRLNKKKI FKGHMVAGYA CQVDFSPDMS YVISGDGNGK LNIWDWKTTK LYSRF KAHD KVCIGAVWHP HETSKVITCG WDGLIKLWD

Macromolecule #41: Pre-mRNA-splicing factor SPF27

• Macromolecule: Name: Pre-mRNA-splicing factor SPF27 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 26.16342 KDa

Sequence

String:

MAGTGLVAGE VVVDALPYFD QGYEAPGVRE AAAALVEEET RRYRPTKNYL SYLTAPDYSA FETDIMRNEF ERLAARQPIE LLSMKRYEL PAPSSGQKND ITAWQECVNN SMAQLEHQAV RIENLELMSQ HGCNAWKVYN ENLVHMIEHA QKELQKLRKH I QDLNWQRK NMQLTAGSKL REMESNWVSL VSKNYEIERT IVQLENEIYQ IKQQHGEANK ENIRQDF

Macromolecule #42: Pre-mRNA-processing factor 19

• Macromolecule: Name: Pre-mRNA-processing factor 19 / type: protein_or_peptide / ID: 42 / Number of copies: 4 / Enantiomer: LEVO / EC number: RING-type E3 ubiquitin transferase
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 55.245547 KDa

Sequence

String:

MSLICSISNE VPEHPCVSPV SNHVYERRLI EKYIAENGTD PINNQPLSEE QLIDIKVAHP IRPKPPSATS IPAILKALQD EWDAVMLHS FTLRQQLQTT RQELSHALYQ HDAACRVIAR LTKEVTAARE ALATLKPQAG LIVPQAVPSS QPSVVGAGEP M DLGELVGM TPEIIQKLQD KATVLTTERK KRGKTVPEEL VKPEELSKYR QVASHVGLHS ASIPGILALD LCPSDTNKIL TG GADKNVV VFDKSSEQIL ATLKGHTKKV TSVVFHPSQD LVFSASPDAT IRIWSVPNAS CVQVVRAHES AVTGLSLHAT GDY LLSSSD DQYWAFSDIQ TGRVLTKVTD ETSGCSLTCA QFHPDGLIFG TGTMDSQIKI WDLKERTNVA NFPGHSGPIT SIAF SENGY YLATAADDSS VKLWDLRKLK NFKTLQLDNN FEVKSLIFDQ SGTYLALGGT DVQIYICKQW TEILHFTEHS GLTTG VAFG HHAKFIASTG MDRSLKFYSL

Macromolecule #43: Pre-mRNA-splicing factor SYF2

• Macromolecule: Name: Pre-mRNA-splicing factor SYF2 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 17.302371 KDa

Sequence

String:

LLEISAEDAE RWERKKKRKN PDLGFSDYAA AQLRQYHRLT KQIKPDMETY ERLREKHGEE FFPTSNSLLH GTHVPSTEEI DRMVIDLEK QIEKRDKYSR RRPYNDDADI DYINERNAKF NKKAERFYGK YTAEIKQNLE RGTAV

Macromolecule #44: Replication stress response regulator SDE2

• Macromolecule: Name: Replication stress response regulator SDE2 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 4.036538 KDa

Sequence

String:

EKTTNREACR DLSGRRLRDV NHEKAMAEWV KQQA

Macromolecule #45: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 45 / Number of copies: 7 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #46: POTASSIUM ION

• Macromolecule: Name: POTASSIUM ION / type: ligand / ID: 46 / Number of copies: 1 / Formula: K
• Molecular weight: Theoretical: 39.098 Da

Macromolecule #47: ADENOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 47 / Number of copies: 1 / Formula: ATP
• Molecular weight: Theoretical: 507.181 Da

Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

Macromolecule #48: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 48 / Number of copies: 1 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

Macromolecule #49: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 49 / Number of copies: 7 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #50: INOSITOL HEXAKISPHOSPHATE

• Macromolecule: Name: INOSITOL HEXAKISPHOSPHATE / type: ligand / ID: 50 / Number of copies: 1 / Formula: IHP
• Molecular weight: Theoretical: 660.035 Da

Chemical component information

ChemComp-IHP:
INOSITOL HEXAKISPHOSPHATE / Phytic acid

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 0.3 mg/mL

Buffer

pH: 7.9
Component:

Concentration	Name	Formula
20.0 mM	HEPES
100.0 mM		KCl
0.2 mM	EDTAEthylenediaminetetraacetic acid

• Grid: Model: Quantifoil R2/2 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 7.0 nm / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Atmosphere: AIR
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK III; Details: 3 uL sample was applied to the grid, left for 25s, then blotted for 2.5-3.5s and immediately plunged into liquid ethane..

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 3.5 µm / Nominal defocus min: 1.5 µm / Nominal magnification: 135000
• Image recording: Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Digitization - Frames/image: 1-40 / Number grids imaged: 2 / Number real images: 6200 / Average exposure time: 10.0 sec. / Average electron dose: 53.0 e/Ų
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Initial angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.0)
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.0)
• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.3 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.0) / Number images used: 103860