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- PDB-2w4v: Isometrically contracting insect asynchronous flight muscle quick... -

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Basic information

Entry
Database: PDB / ID: 2w4v
TitleIsometrically contracting insect asynchronous flight muscle quick frozen after a quick release step
DescriptorMYOSIN HEAVY CHAIN
STRIATED MUSCLE
MYOSIN REGULATORY LIGHT CHAIN
STRIATED ADDUCTOR MUSCLE
MYOSIN ESSENTIAL LIGHT CHAIN
STRIATED ADDUCTOR MUSCLE
KeywordsCONTRACTILE PROTEIN / TROPOMYOSIN / LIGHT CHAINS / ACTIN-BINDING / ISOMETRIC CONTRACTION / THIN FILAMENT / MOTOR PROTEIN / THICK FILAMENT / MUSCLE PROTEIN
Specimen sourceArgopecten irradians / invertebrata / BAY SCALLOP
MethodElectron microscopy (35 A resolution / Tomography / Freeze substituted insect flight muscle)
AuthorsWu, S. / Liu, J. / Reedy, M.C. / Tregear, R.T. / Winkler, H. / Franzini-Armstrong, C. / Sasaki, H. / Lucaveche, C. / Goldman, Y.E. / Reedy, M.K. / Taylor, K.A.
CitationPLoS ONE, 2012, 7, e39422-e39422

primary. PLoS ONE, 2012, 7, e39422-e39422 StrPapers
Structural changes in isometrically contracting insect flight muscle trapped following a mechanical perturbation.
Shenping Wu / Jun Liu / Mary C Reedy / Robert J Perz-Edwards / Richard T Tregear / Hanspeter Winkler / Clara Franzini-Armstrong / Hiroyuki Sasaki / Carmen Lucaveche / Yale E Goldman / Michael K Reedy / Kenneth A Taylor

#1. J. Struct. Biol., 2009, 168, 485-502 StrPapers
Methods for identifying and averaging variable molecular conformations in tomograms of actively contracting insect flight muscle.
Wu, S. / Liu, J. / Reedy, M.C. / Winkler, H. / Reedy, M.K. / Taylor, K.A.

DateDeposition: Dec 2, 2008 / Release: Sep 15, 2010 / Last modification: Apr 19, 2017

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Assembly

Deposited unit
C: MYOSIN HEAVY CHAIN, STRIATED MUSCLE
Y: MYOSIN REGULATORY LIGHT CHAIN, STRIATED ADDUCTOR MUSCLE
Z: MYOSIN ESSENTIAL LIGHT CHAIN, STRIATED ADDUCTOR MUSCLE


Theoretical massNumber of molelcules
Total (without water)127,4713
Polyers127,4713
Non-polymers00
Water0
#1


TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
Conformers
Datacriteria
Number of conformers (submitted / calculated)- / -
Representative

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Components

#1: Polypeptide(L)MYOSIN HEAVY CHAIN, STRIATED MUSCLE / MYOSIN S1


Mass: 94843.883 Da / Num. of mol.: 1 / Details: MYOSIN S1 HEAVY CHAIN / Fragment: RESIDUES 5-835 / Source: (natural) Argopecten irradians / invertebrata / References: UniProt: P24733

Cellular component

Molecular function

#2: Polypeptide(L)MYOSIN REGULATORY LIGHT CHAIN, STRIATED ADDUCTOR MUSCLE / MYOSIN S1 / R-LC


Mass: 15575.700 Da / Num. of mol.: 1
Details: MYOSIN REGULATORY LIGHT CHAIN, RLC, STRIATED ADDUCTOR MUSCLE
Fragment: RESIDUES 16-151 / Source: (natural) Argopecten irradians / invertebrata / References: UniProt: P13543

Cellular component

Molecular function

#3: Polypeptide(L)MYOSIN ESSENTIAL LIGHT CHAIN, STRIATED ADDUCTOR MUSCLE / MYOSIN S1 / E-LC


Mass: 17051.912 Da / Num. of mol.: 1
Details: MYOSIN ESSENTIAL LIGHT CHAIN, ELC, STRIATED ADDUCTOR MUSCLE
Fragment: RESIDUES 5-155 / Source: (natural) Argopecten irradians / invertebrata / References: UniProt: P07291

Cellular component

Molecular function

Sequence details92 IDENTITY TO P24733 100 IDENTITY TO P13543 100 IDENTITY TO P07291

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentReconstruction method: TOMOGRAPHY / Specimen type: FREEZE SUBSTITUTED INSECT FLIGHT MUSCLE

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Sample preparation

Assembly of specimenName: INSECT FIBRILLAR FLIGHT MUSCLE / Aggregation state: TISSUE
Details: THIS SPECIMEN IS OBTAINED FROM A QUICK FROZEN, ISOMETRICALLY CONTRACTING ASYNCHRONOUS INSECT FLIGHT MUSCLE THAT HAS BEEN FREEZE SUBSTITUTED, PLASTIC EMBEDDED, AND THIN SECTIONED. THE FIBERS FOR THIS STUDY WERE SUBJECTED TO MECHANICAL TRANSIENTS. FOR THE QUICK RELEASE,THE FIBER WAS RELEASED 9 NM PER HALF-SARCOMERE IN 2 MS AND THE FREEZING IMPACT - OCC-6 MS LATER.
ComponentName: INSECT FIBRILLAR FLIGHT MUSCLE
Details: THIS SPECIMEN IS OBTAINED FROM A QUICK FROZEN, ISOMETRICALLY CONTRACTING ASYNCHRONOUS INSECT FLIGHT MUSCLE THAT HAS BEEN FREEZE SUBSTITUTED, PLASTIC EMBEDDED, AND THIN SECTIONED. THE FIBERS FOR THIS STUDY WERE SUBJECTED TO MECHANICAL TRANSIENTS. FOR THE QUICK RELEASE,THE FIBER WAS RELEASED 9 NM PER HALF-SARCOMERE IN 2 MS AND THE FREEZING IMPACT - OCC-6 MS LATER.
Type: TISSUE
Buffer solutionName: 20 MM MOPS BUFFER, 5 MM NAN3, AND MGCL2, ATP, CACL2, AND EGTA IN VARYING MILLIMOLAR CONCENTRATIONS
Details: 20 MM MOPS BUFFER, 5 MM NAN3, AND MGCL2, ATP, CACL2, AND EGTA IN VARYING MILLIMOLAR CONCENTRATIONS
Specimen supportDetails: CARBON
VitrificationInstrument: HOMEMADE PLUNGER / Cryogen name: HELIUM
Details: SMASH AGAINST A LIQUID HELIUM COOLED GOLD COATED COPPER MIRROR

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Electron microscopy imaging

MicroscopyMicroscope model: FEI/PHILIPS CM300FEG/T
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Cs: 2 mm
Specimen holderTilt angle max: 72 deg. / Tilt angle min: -72 deg.
CameraType: TIETZ TEM-CAM F224
Radiation wavelengthRelative weight: 1

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Processing

3D reconstructionSoftware: PROTOMO, IMOD / Resolution method: FSC 0.5 CUT-OFF
Details: NOTE THAT OUR LOWEST RESOLUTION DATA IS AT INVERSE 1 MICRON. NUMBER OF FOURIER COEFFICIENTS IS ALMOST A HALF MILLION. THESE COORDINATES WERE FITTED TO AVERAGED SUBVOLUMES OBTAINED FROM A DUAL AXIS TOMOGRAM. THE FITTING WAS DONE MANUALLY USING THE CRYSTALLOGRAPHIC MODEL FITTING PROGRAM O. THERE ARE 27 MODELS.
Least-squares processHighest resolution: 35 A
Refine hist #LASTHighest resolution: 35 A
Number of atoms included #LASTProtein: 8501 / Nucleic acid: 0 / Ligand: 0 / Solvent: 0 / Total: 8501

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