+Open data
-Basic information
Entry | Database: PDB / ID: 3i5f | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Crystal structure of squid MG.ADP myosin S1 | |||||||||
Components |
| |||||||||
Keywords | CONTRACTILE PROTEIN / SQUID / POST-RIGOR STATE / MG.ADP / MYOSIN II S1 / ATP-binding / Actin-binding / Coiled coil / Motor protein / Muscle protein / Myosin / Nucleotide-binding / Thick filament / Calcium | |||||||||
Function / homology | Function and homology information myosin complex / myofibril / cytoskeletal motor activity / actin filament binding / calcium ion binding / ATP binding Similarity search - Function | |||||||||
Biological species | Loligo pealei (longfin inshore squid) Todarodes pacificus (Japanese flying squid) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.1 Å | |||||||||
Authors | Yang, Y. / Gourinath, S. / Kovacs, M. / Nyitray, L. / Reutzel, R. / Himmel, D.M. / O'Neall-Hennessey, E. / Reshetnikova, L. / Szent-Gyorgyi, A.G. / Brown, J.H. / Cohen, C. | |||||||||
Citation | Journal: Structure / Year: 2007 Title: Rigor-like structures from muscle myosins reveal key mechanical elements in the transduction pathways of this allosteric motor. Authors: Yang, Y. / Gourinath, S. / Kovacs, M. / Nyitray, L. / Reutzel, R. / Himmel, D.M. / O'Neall-Hennessey, E. / Reshetnikova, L. / Szent-Gyorgyi, A.G. / Brown, J.H. / Cohen, C. | |||||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 3i5f.cif.gz | 236.6 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb3i5f.ent.gz | 186.3 KB | Display | PDB format |
PDBx/mmJSON format | 3i5f.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/i5/3i5f ftp://data.pdbj.org/pub/pdb/validation_reports/i5/3i5f | HTTPS FTP |
---|
-Related structure data
Related structure data | 2ec6C 2os8C 2otgC 3i5gC 3i5hC 3i5iC 2ovk C: citing same article (ref.) S: Starting model for refinement |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 95939.445 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Loligo pealei (longfin inshore squid) / References: UniProt: O44934 |
---|---|
#2: Protein | Mass: 17577.662 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Todarodes pacificus (Japanese flying squid) References: UniProt: P08052 |
#3: Protein | Mass: 18081.236 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) Todarodes pacificus (Japanese flying squid) References: UniProt: P05945 |
#4: Chemical | ChemComp-MG / |
#5: Chemical | ChemComp-ADP / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
---|
-Sample preparation
Crystal | Density Matthews: 2.98 Å3/Da / Density % sol: 58.7 % |
---|---|
Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7 Details: pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: CHESS / Beamline: A1 |
Detector | Date: Nov 5, 2005 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 3.1→43.23 Å / Num. obs: 25326 / % possible obs: 88.8 % / Redundancy: 3.9 % / Rmerge(I) obs: 0.111 / Net I/σ(I): 10.2 |
Reflection shell | Resolution: 3.1→3.21 Å / Redundancy: 1.7 % / Rmerge(I) obs: 0.287 / Mean I/σ(I) obs: 2.1 / % possible all: 41.5 |
-Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB entry 2OVK 2ovk Resolution: 3.1→43.23 Å / σ(F): 0
| ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.1→43.23 Å
| ||||||||||||||||||||
Refine LS restraints |
|