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Open data
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Basic information
Entry | Database: PDB / ID: 3i5f | |||||||||
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Title | Crystal structure of squid MG.ADP myosin S1 | |||||||||
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![]() | CONTRACTILE PROTEIN / SQUID / POST-RIGOR STATE / MG.ADP / MYOSIN II S1 / ATP-binding / Actin-binding / Coiled coil / Motor protein / Muscle protein / Myosin / Nucleotide-binding / Thick filament / Calcium | |||||||||
Function / homology | ![]() cytoskeletal motor regulator activity / mitotic actomyosin contractile ring / mitotic actomyosin contractile ring contraction / muscle myosin complex / myosin filament / actomyosin structure organization / locomotion / myosin II complex / microfilament motor activity / myofibril ...cytoskeletal motor regulator activity / mitotic actomyosin contractile ring / mitotic actomyosin contractile ring contraction / muscle myosin complex / myosin filament / actomyosin structure organization / locomotion / myosin II complex / microfilament motor activity / myofibril / sarcomere organization / myosin heavy chain binding / mitotic cytokinesis / post-embryonic development / muscle contraction / actin filament binding / calcium ion binding / ATP binding / metal ion binding / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Yang, Y. / Gourinath, S. / Kovacs, M. / Nyitray, L. / Reutzel, R. / Himmel, D.M. / O'Neall-Hennessey, E. / Reshetnikova, L. / Szent-Gyorgyi, A.G. / Brown, J.H. / Cohen, C. | |||||||||
![]() | ![]() Title: Rigor-like structures from muscle myosins reveal key mechanical elements in the transduction pathways of this allosteric motor. Authors: Yang, Y. / Gourinath, S. / Kovacs, M. / Nyitray, L. / Reutzel, R. / Himmel, D.M. / O'Neall-Hennessey, E. / Reshetnikova, L. / Szent-Gyorgyi, A.G. / Brown, J.H. / Cohen, C. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 236.6 KB | Display | ![]() |
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PDB format | ![]() | 186.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 825 KB | Display | ![]() |
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Full document | ![]() | 951.8 KB | Display | |
Data in XML | ![]() | 58 KB | Display | |
Data in CIF | ![]() | 77.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2ec6C ![]() 2os8C ![]() 2otgC ![]() 3i5gC ![]() 3i5hC ![]() 3i5iC ![]() 2ovk C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 95939.445 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
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#2: Protein | Mass: 17577.662 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) ![]() References: UniProt: P08052 |
#3: Protein | Mass: 18081.236 Da / Num. of mol.: 1 / Source method: isolated from a natural source Source: (natural) ![]() References: UniProt: P05945 |
#4: Chemical | ChemComp-MG / |
#5: Chemical | ChemComp-ADP / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.98 Å3/Da / Density % sol: 58.7 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7 Details: pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Date: Nov 5, 2005 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 3.1→43.23 Å / Num. obs: 25326 / % possible obs: 88.8 % / Redundancy: 3.9 % / Rmerge(I) obs: 0.111 / Net I/σ(I): 10.2 |
Reflection shell | Resolution: 3.1→3.21 Å / Redundancy: 1.7 % / Rmerge(I) obs: 0.287 / Mean I/σ(I) obs: 2.1 / % possible all: 41.5 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 2OVK ![]() 2ovk Resolution: 3.1→43.23 Å / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 3.1→43.23 Å
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Refine LS restraints |
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