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- PDB-2os8: Rigor-like structures of muscle myosins reveal key mechanical ele... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2os8 | ||||||
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Title | Rigor-like structures of muscle myosins reveal key mechanical elements in the transduction pathways of this allosteric motor | ||||||
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![]() | CONTRACTILE PROTEIN / myosin S1 / rigor-like / motor / mechanical elements | ||||||
Function / homology | ![]() cytoskeletal motor regulator activity / mitotic actomyosin contractile ring / mitotic actomyosin contractile ring contraction / muscle myosin complex / myosin filament / actomyosin structure organization / locomotion / myosin II complex / microfilament motor activity / myofibril ...cytoskeletal motor regulator activity / mitotic actomyosin contractile ring / mitotic actomyosin contractile ring contraction / muscle myosin complex / myosin filament / actomyosin structure organization / locomotion / myosin II complex / microfilament motor activity / myofibril / sarcomere organization / myosin heavy chain binding / mitotic cytokinesis / post-embryonic development / muscle contraction / actin filament binding / calcium ion binding / ATP binding / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Yang, Y. / Gourinath, S. / Cohen, C. / Brown, J.H. | ||||||
![]() | ![]() Title: Rigor-like Structures from Muscle Myosins Reveal Key Mechanical Elements in the Transduction Pathways of This Allosteric Motor. Authors: Yang, Y. / Gourinath, S. / Kovacs, M. / Nyitray, L. / Reutzel, R. / Himmel, D.M. / O'neall-Hennessey, E. / Reshetnikova, L. / Szent-Gyorgyi, A.G. / Brown, J.H. / Cohen, C. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 230.3 KB | Display | ![]() |
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PDB format | ![]() | 180.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 448.4 KB | Display | ![]() |
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Full document | ![]() | 562.2 KB | Display | |
Data in XML | ![]() | 53.3 KB | Display | |
Data in CIF | ![]() | 71.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2ec6C ![]() 2otgC ![]() 3i5fC ![]() 3i5gC ![]() 3i5hC ![]() 3i5iC ![]() 1sr6S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 96139.289 Da / Num. of mol.: 1 / Fragment: Myosin heavy chain / Source method: isolated from a natural source / Source: (natural) ![]() |
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#2: Protein | Mass: 17769.146 Da / Num. of mol.: 1 / Fragment: Myosin RLC / Source method: isolated from a natural source / Source: (natural) ![]() |
#3: Protein | Mass: 17811.805 Da / Num. of mol.: 1 / Fragment: Myosin ELC / Source method: isolated from a natural source / Source: (natural) ![]() |
#4: Chemical | ChemComp-MG / |
#5: Chemical | ChemComp-CA / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.61 Å3/Da / Density % sol: 52.91 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: CaCl2, PEG , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 3.25→50 Å / Num. all: 16888 / Num. obs: 16888 / % possible obs: 79.6 % / Observed criterion σ(I): -3 / Redundancy: 3.5 % / Biso Wilson estimate: 66 Å2 / Rsym value: 0.085 / Net I/σ(I): 7.2 |
Reflection shell | Resolution: 3.25→3.37 Å / Redundancy: 2.5 % / Mean I/σ(I) obs: 1.5 / Num. unique all: 1371 / Rsym value: 0.451 / % possible all: 64.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1SR6 Resolution: 3.27→48.03 Å / Rfactor Rfree error: 0.009 / Data cutoff high absF: 125059.531 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 117.409 Å2 / ksol: 0.261 e/Å3 | ||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 102.2 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3.27→48.03 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.25→3.45 Å / Rfactor Rfree error: 0.033 / Total num. of bins used: 6
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Xplor file |
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