登録情報 データベース : EMDB / ID : EMD-25126 構造の表示 ダウンロードとリンクタイトル Cryo-EM structure of GPR158 coupled to the RGS7-Gbeta5 complex マップデータ 詳細 試料複合体 : GPCR complexタンパク質・ペプチド : Isoform 2 of Regulator of G-protein signaling 7タンパク質・ペプチド : Guanine nucleotide-binding protein subunit beta-5タンパク質・ペプチド : G-protein coupled receptor 158リガンド : CHOLESTEROLリガンド : (2S)-1-{[(S)-hydroxy{[(1s,2R,3R,4R,5S,6S)-2,3,4,5,6-pentahydroxycyclohexyl]oxy}phosphoryl]oxy}-3-(octadecanoyloxy)propan-2-yl (5E,8E,11E,14E)-icosa-5,8,11,14-tetraenoateリガンド : 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine 詳細機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
G protein-coupled glycine receptor activity / Inactivation, recovery and regulation of the phototransduction cascade / G beta:gamma signalling through PLC beta / Presynaptic function of Kainate receptors / Prostacyclin signalling through prostacyclin receptor / G alpha (z) signalling events / Glucagon-type ligand receptors / G beta:gamma signalling through PI3Kgamma / G beta:gamma signalling through CDC42 / G beta:gamma signalling through BTK ... G protein-coupled glycine receptor activity / Inactivation, recovery and regulation of the phototransduction cascade / G beta:gamma signalling through PLC beta / Presynaptic function of Kainate receptors / Prostacyclin signalling through prostacyclin receptor / G alpha (z) signalling events / Glucagon-type ligand receptors / G beta:gamma signalling through PI3Kgamma / G beta:gamma signalling through CDC42 / G beta:gamma signalling through BTK / Adrenaline,noradrenaline inhibits insulin secretion / ADP signalling through P2Y purinoceptor 12 / light adaption / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / dark adaptation / Thromboxane signalling through TP receptor / G-protein gamma-subunit binding / Thrombin signalling through proteinase activated receptors (PARs) / Activation of G protein gated Potassium channels / Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits / G-protein activation / Ca2+ pathway / G alpha (s) signalling events / G alpha (q) signalling events / Extra-nuclear estrogen signaling / negative regulation of voltage-gated calcium channel activity / G alpha (12/13) signalling events / cell tip / Vasopressin regulates renal water homeostasis via Aquaporins / G alpha (i) signalling events / GPER1 signaling / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / ADP signalling through P2Y purinoceptor 1 / positive regulation of potassium ion transmembrane transport / regulation of G protein-coupled receptor signaling pathway / GTPase activating protein binding / parallel fiber to Purkinje cell synapse / positive regulation of neurotransmitter secretion / G protein-coupled dopamine receptor signaling pathway / negative regulation of G protein-coupled receptor signaling pathway / regulation of synapse organization / G-protein alpha-subunit binding / enzyme activator activity / response to amphetamine / GTPase activator activity / positive regulation of GTPase activity / protein localization to plasma membrane / cell projection / brain development / transmembrane signaling receptor activity / cognition / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / nuclear envelope / G-protein beta-subunit binding / heterotrimeric G-protein complex / signaling receptor complex adaptor activity / myelin sheath / presynapse / presynaptic membrane / protein-folding chaperone binding / G alpha (i) signalling events / cell body / postsynaptic membrane / response to ethanol / intracellular signal transduction / neuron projection / G protein-coupled receptor signaling pathway / GTPase activity / synapse / dendrite / protein-containing complex / nucleus / plasma membrane / cytoplasm / cytosol 類似検索 - 分子機能 : / : / G-protein coupled receptor 158/179 / Regulator of G-protein signalling, DHEX domain / Regulator of G-protein signalling DHEX domain / Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP) / DEP domain profile. / Domain found in Dishevelled, Egl-10, and Pleckstrin / DEP domain / Regulator of G protein signaling domain ... : / : / G-protein coupled receptor 158/179 / Regulator of G-protein signalling, DHEX domain / Regulator of G-protein signalling DHEX domain / Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP) / DEP domain profile. / Domain found in Dishevelled, Egl-10, and Pleckstrin / DEP domain / Regulator of G protein signaling domain / RGS, subdomain 2 / RGS domain / RGS domain profile. / Regulator of G protein signalling domain / RGS domain superfamily / G-protein coupled receptors family 3 profile. / GPCR family 3, C-terminal / 7 transmembrane sweet-taste receptor of 3 GCPR / GGL domain / G-protein gamma-like domain superfamily / G-protein gamma-like domain / GGL domain / G protein gamma subunit-like motifs / Guanine nucleotide-binding protein, beta subunit / G-protein, beta subunit / G-protein beta WD-40 repeat / Winged helix DNA-binding domain superfamily / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / Winged helix-like DNA-binding domain superfamily / WD40/YVTN repeat-like-containing domain superfamily 類似検索 - ドメイン・相同性 Regulator of G-protein signaling 7 / Guanine nucleotide-binding protein subunit beta-5 / Metabotropic glycine receptor 類似検索 - 構成要素生物種 Homo sapiens (ヒト) / Mus musculus (ハツカネズミ)手法 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 3.4 Å 詳細 データ登録者Patil DN / Singh S / Singh AK / Martemyanov KA 資金援助 米国, 1件 詳細 詳細を隠すOrganization Grant number 国 National Institutes of Health/National Institute of Mental Health (NIH/NIMH) 米国
引用ジャーナル : Science / 年 : 2022タイトル : Cryo-EM structure of human GPR158 receptor coupled to the RGS7-Gβ5 signaling complex.著者: Dipak N Patil / Shikha Singh / Thibaut Laboute / Timothy S Strutzenberg / Xingyu Qiu / Di Wu / Scott J Novick / Carol V Robinson / Patrick R Griffin / John F Hunt / Tina Izard / Appu K Singh ... 著者 : Dipak N Patil / Shikha Singh / Thibaut Laboute / Timothy S Strutzenberg / Xingyu Qiu / Di Wu / Scott J Novick / Carol V Robinson / Patrick R Griffin / John F Hunt / Tina Izard / Appu K Singh / Kirill A Martemyanov / 要旨 : GPR158 is an orphan G protein–coupled receptor (GPCR) highly expressed in the brain, where it controls synapse formation and function. GPR158 has also been implicated in depression, carcinogenesis, ... GPR158 is an orphan G protein–coupled receptor (GPCR) highly expressed in the brain, where it controls synapse formation and function. GPR158 has also been implicated in depression, carcinogenesis, and cognition. However, the structural organization and signaling mechanisms of GPR158 are largely unknown. We used single-particle cryo–electron microscopy (cryo-EM) to determine the structures of human GPR158 alone and bound to an RGS signaling complex. The structures reveal a homodimeric organization stabilized by a pair of phospholipids and the presence of an extracellular Cache domain, an unusual ligand-binding domain in GPCRs. We further demonstrate the structural basis of GPR158 coupling to RGS7-Gβ5. Together, these results provide insights into the unusual biology of orphan receptors and the formation of GPCR-RGS complexes. 履歴 登録 2021年10月8日 - ヘッダ(付随情報) 公開 2021年12月1日 - マップ公開 2021年12月1日 - 更新 2022年1月19日 - 現状 2022年1月19日 処理サイト : RCSB / 状態 : 公開
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