EMDB-24664: Composite cryo-EM density map of the 48-nm repeat doublet microtu...

Basic information

• Entry: Database: EMDB / ID: EMD-24664
• Title: Composite cryo-EM density map of the 48-nm repeat doublet microtubule from bovine tracheal cilia

Sample

• Complex: Doublet microtubule
  • Protein or peptide: x 36 types
• Ligand: x 3 types

• Keywords: cilia / microtubule / dynein / motility / STRUCTURAL PROTEIN

Function / homology

Function:

regulation of cilium beat frequency / mucociliary clearance / outer dynein arm docking complex / sperm flagellum assembly / outer acrosomal membrane / regulation of brood size / establishment of left/right asymmetry / protein localization to motile cilium / manchette assembly / axonemal B tubule inner sheath / axonemal A tubule inner sheath / epithelial cilium movement involved in determination of left/right asymmetry / protein polyglutamylation / positive regulation of feeding behavior / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Cilium Assembly / Intraflagellar transport / Carboxyterminal post-translational modifications of tubulin / Sealing of the nuclear envelope (NE) by ESCRT-III / Kinesins / cerebrospinal fluid circulation / Resolution of Sister Chromatid Cohesion / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / outer dynein arm assembly / sperm axoneme assembly / cilium-dependent cell motility / COPI-dependent Golgi-to-ER retrograde traffic / RHO GTPases activate IQGAPs / COPI-independent Golgi-to-ER retrograde traffic / COPI-mediated anterograde transport / RHO GTPases Activate Formins / regulation of cilium beat frequency involved in ciliary motility / cilium movement involved in cell motility / 9+2 motile cilium / Transferases; Transferring phosphorus-containing groups / acrosomal membrane / regulation of cilium assembly / MHC class II antigen presentation / ciliary transition zone / cilium movement / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / axoneme assembly / axonemal microtubule / Aggrephagy / left/right axis specification / cilium organization / gamma-tubulin ring complex / The role of GTSE1 in G2/M progression after G2 checkpoint / flagellated sperm motility / Separation of Sister Chromatids / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / Anchoring of the basal body to the plasma membrane / AURKA Activation by TPX2 / Recruitment of NuMA to mitotic centrosomes / ventricular system development / manchette / Regulation of PLK1 Activity at G2/M Transition / Hedgehog 'off' state / positive regulation of cilium assembly / CTP biosynthetic process / UTP biosynthetic process / motile cilium / positive regulation of cell motility / determination of left/right symmetry / GTP biosynthetic process / Neutrophil degranulation / microtubule organizing center / intermediate filament / regulation of neuron projection development / ciliary base / nucleoside diphosphate kinase activity / beta-tubulin binding / axoneme / centriolar satellite / spermatid development / mitotic cytokinesis / alpha-tubulin binding / cilium assembly / single fertilization / cellular response to UV-C / sperm flagellum / sperm midpiece / Hsp70 protein binding / centriole / 3'-5' exonuclease activity / mitotic spindle organization / acrosomal vesicle / ciliary basal body / G protein-coupled receptor binding / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / lung development / Hsp90 protein binding / protein localization / brain development / structural constituent of cytoskeleton / mitotic spindle / cilium

Domain/homology:

Outer dynein arm-docking complex subunit 4 / Outer dynein arm-docking complex subunit 3 / MalT-like TPR region / MalT-like TPR region / Tektin bundle interacting protein 1 / Tektin bundle interacting protein 1 / Sperm-associated antigen 8 / Cilia- and flagella-associated protein 95 / NDPK7, first NDPk domain / Cilia- and flagella-associated protein 107 / : / Protein CFAP95 / Tektin / Cilia- and flagella-associated protein 276 / Tektin family / Protein of unknown function (DUF3695) / Uncharacterised protein FAM166/UPF0605 / Protein Flattop / Ciliary microtubule inner protein 2B-like / Nucleoside diphosphate kinase 7 / Meiosis-specific nuclear structural protein 1 / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 45 / NDPK7, second NDPk domain / Cilia- and flagella- associated protein 210 / CFAP53/TCHP / Trichohyalin-plectin-homology domain / Trichohyalin-plectin-homology domain / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 141 / RIB43A / RIB43A / DM10 domain / EF-hand domain-containing protein EFHC1/EFHC2/EFHB / DM10 domain / DM10 domain profile. / Domains in hypothetical proteins in Drosophila, C. elegans and mammals. Occurs singly in some nucleoside diphosphate kinases. / Enkurin domain / Calmodulin-binding / Enkurin domain profile. / CFA20 domain / Cilia- and flagella-associated protein 20/CFAP20DC / CFA20 domain / Parkin co-regulated protein / Parkin co-regulated protein / Recoverin family / : / Nucleoside diphosphate kinase (NDPK)-like domain profile. / EF hand / Nucleoside diphosphate kinase / Nucleoside diphosphate kinase-like domain / Nucleoside diphosphate kinase / NDK / Nucleoside diphosphate kinase-like domain superfamily / Armadillo/plakoglobin ARM repeat profile. / Armadillo/beta-catenin-like repeat / Armadillo/beta-catenin-like repeats / Armadillo / TPR repeat region circular profile. / Alpha tubulin / Tubulin-beta mRNA autoregulation signal. / Beta tubulin, autoregulation binding site / Beta tubulin / TPR repeat profile. / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tetratricopeptide repeats / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily / Tetratricopeptide repeat / EF-hand domain pair / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / Armadillo-like helical / EF-hand domain pair / Tetratricopeptide-like helical domain superfamily / Armadillo-type fold / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily

Component:

Piercer of microtubule wall 2 protein / EF-hand domain-containing family member C2 / TTC25 protein / Parkin coregulated gene protein / Tektin-3 / Outer dynein arm-docking complex subunit 3 / Enkurin / Armadillo repeat containing 4 / Cilia- and flagella-associated protein 276 / Cilia- and flagella- associated protein 210 / Cilia- and flagella-associated protein 52 / EF-hand domain-containing protein 1 / Sperm-associated antigen 8 / Meiosis-specific nuclear structural protein 1 / EF-hand domain-containing family member B / Coiled-coil domain containing 114 / Cilia- and flagella-associated protein 53 / Cilia- and flagella-associated protein 161 / Tubulin alpha-1D chain / Uncharacterized protein C19orf71 homolog / Tektin-2 / Cilia- and flagella-associated protein 107 / Tektin-4 / Protein FAM166B / Tektin-1 / EF-hand calcium-binding domain-containing protein 1 / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 95 / RIB43A-like with coiled-coils protein 2 / Cilia- and flagella-associated protein 45 / Piercer of microtubule wall 1 protein / Tubulin beta-4B chain / Protein Flattop / Nucleoside diphosphate kinase homolog 7 / Cilia- and flagella-associated protein 20

• Biological species: Bos taurus (cattle)
• Method: single particle reconstruction / cryo EM / Resolution: 3.4 Å
• Authors: Gui M / Anderson JR / Botsch JJ / Meleppattu S / Singh SK / Zhang Q / Brown A

Funding support

United States, 1 items

Organization	Grant number	Country
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)		United States

• Citation: Journal: Cell / Year: 2021; Title: De novo identification of mammalian ciliary motility proteins using cryo-EM.; Authors: Miao Gui / Hannah Farley / Priyanka Anujan / Jacob R Anderson / Dale W Maxwell / Jonathan B Whitchurch / J Josephine Botsch / Tao Qiu / Shimi Meleppattu / Sandeep K Singh / Qi Zhang / James Thompson / Jane S Lucas / Colin D Bingle / Dominic P Norris / Sudipto Roy / Alan Brown / ; Abstract: Dynein-decorated doublet microtubules (DMTs) are critical components of the oscillatory molecular machine of cilia, the axoneme, and have luminal surfaces patterned periodically by microtubule inner proteins (MIPs). Here we present an atomic model of the 48-nm repeat of a mammalian DMT, derived from a cryoelectron microscopy (cryo-EM) map of the complex isolated from bovine respiratory cilia. The structure uncovers principles of doublet microtubule organization and features specific to vertebrate cilia, including previously unknown MIPs, a luminal bundle of tektin filaments, and a pentameric dynein-docking complex. We identify a mechanism for bridging 48- to 24-nm periodicity across the microtubule wall and show that loss of the proteins involved causes defective ciliary motility and laterality abnormalities in zebrafish and mice. Our structure identifies candidate genes for diagnosis of ciliopathies and provides a framework to understand their functions in driving ciliary motility.

History

Deposition	Aug 10, 2021	-
Header (metadata) release	Oct 27, 2021	-
Map release	Oct 27, 2021	-
Update	Jun 5, 2024	-
Current status	Jun 5, 2024	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_24664.map.gz / Format: CCP4 / Size: 1.1 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.09 Å

Density

Contour Level	By AUTHOR: 7.0 / Movie #1: 7
Minimum - Maximum	0.0 - 49.831657
Average (Standard dev.)	0.28448406 (±1.4580382)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 672 672 672 Spacing 672 672 672
Cell	A=B=C: 732.48004 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.09	1.09	1.09
M x/y/z	672	672	672
origin x/y/z	0.000	0.000	0.000
length x/y/z	732.480	732.480	732.480
α/β/γ	90.000	90.000	90.000
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	672	672	672
D min/max/mean	0.000	49.832	0.284

Supplemental data

Mask #1

• File: emd_24664_msk_1.map

Additional map: consensus map

• File: emd_24664_additional_1.map
• Annotation: consensus map

Additional map: local refined fragment 6-1

• File: emd_24664_additional_10.map
• Annotation: local refined fragment 6-1

Additional map: local refined fragment 6-3

• File: emd_24664_additional_11.map
• Annotation: local refined fragment 6-3

Additional map: local refined fragment 8-1

• File: emd_24664_additional_12.map
• Annotation: local refined fragment 8-1

Additional map: local refined fragment 4-3

• File: emd_24664_additional_13.map
• Annotation: local refined fragment 4-3

Additional map: local refined fragment 2-2

• File: emd_24664_additional_14.map
• Annotation: local refined fragment 2-2

Additional map: local refined fragment 2-3

• File: emd_24664_additional_15.map
• Annotation: local refined fragment 2-3

Additional map: local refined fragment 3-3

• File: emd_24664_additional_16.map
• Annotation: local refined fragment 3-3

Additional map: local refined fragment 10-3

• File: emd_24664_additional_17.map
• Annotation: local refined fragment 10-3

Additional map: local refined fragment 4-1

• File: emd_24664_additional_18.map
• Annotation: local refined fragment 4-1

Additional map: deepemhancer postprocessing map

• File: emd_24664_additional_19.map
• Annotation: deepemhancer postprocessing map

Additional map: local refined fragment of 8 tektin filaments 1

• File: emd_24664_additional_2.map
• Annotation: local refined fragment of 8 tektin filaments 1

Additional map: local refined fragment 10-1

• File: emd_24664_additional_20.map
• Annotation: local refined fragment 10-1

Additional map: local refined fragment 4-2

• File: emd_24664_additional_21.map
• Annotation: local refined fragment 4-2

Additional map: local refined fragment 1-2

• File: emd_24664_additional_22.map
• Annotation: local refined fragment 1-2

Additional map: local refined fragment of 8 tektin filaments 3

• File: emd_24664_additional_23.map
• Annotation: local refined fragment of 8 tektin filaments 3

Additional map: local refined fragment 7-3

• File: emd_24664_additional_24.map
• Annotation: local refined fragment 7-3

Additional map: local refined fragment 2-1

• File: emd_24664_additional_25.map
• Annotation: local refined fragment 2-1

Additional map: local refined fragment 3-2

• File: emd_24664_additional_26.map
• Annotation: local refined fragment 3-2

Additional map: local refined fragment 8-2

• File: emd_24664_additional_27.map
• Annotation: local refined fragment 8-2

Additional map: local refined fragment 7-1

• File: emd_24664_additional_28.map
• Annotation: local refined fragment 7-1

Additional map: local refined fragment 10-2

• File: emd_24664_additional_29.map
• Annotation: local refined fragment 10-2

Additional map: local refined fragment 8-3

• File: emd_24664_additional_3.map
• Annotation: local refined fragment 8-3

Additional map: local refined fragment 5-2

• File: emd_24664_additional_30.map
• Annotation: local refined fragment 5-2

Additional map: local refined fragment 3-1

• File: emd_24664_additional_31.map
• Annotation: local refined fragment 3-1

Additional map: local refined fragment 9-2

• File: emd_24664_additional_32.map
• Annotation: local refined fragment 9-2

Additional map: local refined fragment 9-1

• File: emd_24664_additional_33.map
• Annotation: local refined fragment 9-1

Additional map: local refined fragment of Pierce1/2 region 2

• File: emd_24664_additional_34.map
• Annotation: local refined fragment of Pierce1/2 region 2

Additional map: multi-body local refined fragment of ODA-DC

• File: emd_24664_additional_35.map
• Annotation: multi-body local refined fragment of ODA-DC

Additional map: local refined fragment 1-3

• File: emd_24664_additional_36.map
• Annotation: local refined fragment 1-3

Additional map: local refined fragment of Pierce1/2 region 1

• File: emd_24664_additional_37.map
• Annotation: local refined fragment of Pierce1/2 region 1

Additional map: local refined fragment 9-3

• File: emd_24664_additional_38.map
• Annotation: local refined fragment 9-3

Additional map: Overall cylinder mask

• File: emd_24664_additional_39.map
• Annotation: Overall cylinder mask

Additional map: local refined fragment of 8 tektin filaments 2

• File: emd_24664_additional_4.map
• Annotation: local refined fragment of 8 tektin filaments 2

Additional map: local refined fragment 6-2

• File: emd_24664_additional_5.map
• Annotation: local refined fragment 6-2

Additional map: local refined fragment 5-3

• File: emd_24664_additional_6.map
• Annotation: local refined fragment 5-3

Additional map: local refined fragment 7-2

• File: emd_24664_additional_7.map
• Annotation: local refined fragment 7-2

Additional map: local refined fragment 5-1

• File: emd_24664_additional_8.map
• Annotation: local refined fragment 5-1

Additional map: local refined fragment 1-1

• File: emd_24664_additional_9.map
• Annotation: local refined fragment 1-1

Sample components

Entire : Doublet microtubule

• Entire: Name: Doublet microtubule

Components

• Complex: Doublet microtubule
  • Protein or peptide: Protein C9orf135 homolog
  • Protein or peptide: EF-hand domain family member B
  • Protein or peptide: Methyl-CpG binding domain protein 1
  • Protein or peptide: Nucleoside diphosphate kinase 7
  • Protein or peptide: Uncharacterized protein C1orf158 homolog
  • Protein or peptide: Meiosis-specific nuclear structural protein 1
  • Protein or peptide: Tektin-1
  • Protein or peptide: Tubulin alpha-1D chain
  • Protein or peptide: Tubulin beta-4B chain
  • Protein or peptide: Tektin-2
  • Protein or peptide: Uncharacterized protein C1orf189 homolog
  • Protein or peptide: Tektin-3
  • Protein or peptide: Sperm associated antigen 8
  • Protein or peptide: Tektin-4
  • Protein or peptide: Cilia and flagella associated protein 161
  • Protein or peptide: TEKTIP1
  • Protein or peptide: Pierce2
  • Protein or peptide: Protein FAM166B
  • Protein or peptide: Coiled-coil domain containing 114
  • Protein or peptide: Outer dynein arm-docking complex subunit 3
  • Protein or peptide: Armadillo repeat containing 4
  • Protein or peptide: TTC25 protein
  • Protein or peptide: EF-hand calcium-binding domain-containing protein 1
  • Protein or peptide: RIB43A-like with coiled-coils protein 2
  • Protein or peptide: EF-hand domain containing 1
  • Protein or peptide: EFHC2
  • Protein or peptide: Cilia- and flagella-associated protein 20
  • Protein or peptide: PACRG protein
  • Protein or peptide: Cilia and flagella associated protein 45
  • Protein or peptide: Cilia and flagella associated protein 52
  • Protein or peptide: Enkurin, TRPC channel interacting protein
  • Protein or peptide: Protein Flattop
  • Protein or peptide: Coiled-coil domain containing 173
  • Protein or peptide: Chromosome 3 C1orf194 homolog
  • Protein or peptide: EFCAB6
  • Protein or peptide: Pierce1
• Ligand: GUANOSINE-5'-TRIPHOSPHATE
• Ligand: MAGNESIUM ION
• Ligand: GUANOSINE-5'-DIPHOSPHATE

Supramolecule #1: Doublet microtubule

• Supramolecule: Name: Doublet microtubule / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#36
• Source (natural): Organism: Bos taurus (cattle)

Macromolecule #1: Protein C9orf135 homolog

• Macromolecule: Name: Protein C9orf135 homolog / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 26.661025 KDa

Sequence

String:

MDSSDSSCQE WCSHGQYWLE LGPTDLMERK GSLTLRSHHK KYSKPVLVYS WHRDRETYPK DYDIEGPEEV KKLCNSTYRR LGTSEPPVW ISETREKMAQ VCLNTKLAKI KSKALLNEET MNSGIIERDT GLPATGFGAL FTRHSPDWSK MCTLTTYAEE Y APPYEYQP LGDPCQDDDY SIVHRKCRSQ FTDLDGSKRV GINTWHDESG IYANSYVKRK LYSLTGGPIA PFLK

UniProtKB: Cilia- and flagella-associated protein 95

Macromolecule #2: EF-hand domain family member B

• Macromolecule: Name: EF-hand domain family member B / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 97.960547 KDa

Sequence

String:

MCSFLRVPSP KGLEPSKGLG APLQPGTAYP SEMSALGPGM TVSGRKSPLS MEVGLAPEGK DDLEERRVFM GTTSPTELGL RVGLRKDFP CSKTYEERMA SPEIRSPSSK GLELRLKRQN ISRTVMDGSN LGVHRVSASQ ETKPPLSALP GRVGLEKERF L AGYCPGRV TQPPLGQGCE SPQASGGRRC PVTGDPEGFG ASVSKLPALG MECRGELGGE SACVVMKPRA ETEPPVEVDM GL TRLEEPA EMESPEPQMG LVMEPPAWQL AQQPEEQREA ENTEPGVEPP DRIRPIYSGK FFDRMPCWPS AGKVLPIGYR AAT CLTERF PRLMTPPEAK KFFNFRYPPA GAERVFYGRA NDPQIAPSLT HGIRSKISIP AKVLINPQPI TTFQQKMKDK KESV YFSNQ RAPLGKSHDQ TPGLPKGLDI LNTTFGTAIV RETSARDMVN PPKPYKEVFE EAQAGHDLYV VSHNDYFVGE AKNRK YDPS SFHRFNLYGI PTPHFNDGRN MAKTLHWLHE LQMKRGAKIV SKRVDDFKEK FQHRLGRVLD PIAETMNVPP DYTFGA FLR PEDYGVGDLI HRRLPGEYLR GKDRQRGLVA AVRHHLKKVN YQNFDTLLAA FRHYDKKGDG VIDRAELQEA CDQACLH LD EKLLDQLFEY CDVDKDGLIN YLEFANFLTW KDKTPLKEYE ERVLIKGRKA DCANPAEANV EESEPALLLK PEDIVLKE P GSSEKTLRTL LRPSDKVSNH YKTTSSEISA VVGAVPSTCY PTYGVPTIRS DIPAPLIRRV SDRTSYGEEG NAYSLLHPT IFAQKGVFER DFFKTRSKQE ISEILCNIGV KLSEDEFENV WNLASKKHHR GEVCVENIRS VLDELQHADG AKCKAAT

UniProtKB: EF-hand domain-containing family member B

Macromolecule #3: Methyl-CpG binding domain protein 1

• Macromolecule: Name: Methyl-CpG binding domain protein 1 / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 62.138992 KDa

Sequence

String:

MYSQRFGIVQ REVKGPTPKV VIVRAKPPKG QGAEHHLQRI QHSHQKHHAI LASIKSIERD RLKTEWDQHN DCKFVDSLVK ARVKDAMQG FIINTEERRN KLRELLASEE NEYFTEMQLK EETIEEKKDR MRDKIRLLRE KKEKERQDFV AEKLDQQFRE R CQELRAEL FCIHQKAVCE ERKAQIAFNE ELKRQKVVEE QMFSKLWEED RLAKERREAK EERRQKELVE NTRLGLNAQV TS IQAQRQA AQRLKEEEAL LVENENAQVK LENEQDKLKK QKTKQEIRAA LQKALQEKME RMQQEYREEQ DLNMKLMQNA LQS LQEETD KKKQKKEDMR REQKIYYQYL AQRHEEEKAQ EKELDRMLEK EKEKKFAEKD KELRLEKEAR KQLLNEVMCT RKLQ VQEKL QRKAKEQEER TMEQERINEG LKELNCEERE NFIRRCSLAQ EYRKQLQMQI CSQQQAREAE EEEERREFEA GIAAE KSFQ DKIQGILSTH QVVPRNIHPM RRACSTKLPP

UniProtKB: Cilia- and flagella-associated protein 53

Macromolecule #4: Nucleoside diphosphate kinase 7

• Macromolecule: Name: Nucleoside diphosphate kinase 7 / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO / EC number: nucleoside-diphosphate kinase
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 42.650895 KDa

Sequence

String:

MNHSERFVFI AEWFDPNASL FRRYELLFYP GDGSVEMHDV KNHRTFLKRT KYEDLHLEDL FIGNKVNIFS RQLVLLDYGD QYTARQLGS KKEKTLALIK PDAVSKAGEI IEIINKAGFT LTKLKMMTLS RKEATDFHID HQSRPFLNEL IQFITSGPII A MEILRDDA VCEWKRLLGP ANSGLARTDA PESIRALFGT DGIKNAAHGP DSFACAAREM ELFFPSSGVC GPANTAKFTN CT TCCIVKP HAVSEGLLGK ILMTIRDAGF EISAMQMFNM DRINVEEFYE VYKGVVSEYN EMVTEMYSGP CVAMEIQQTN PTM TFREFC GPADPEIARH LRPGTLRAIF GKTKIQNAVH CTDLPEDGLL EVQYFFKILD N

UniProtKB: Nucleoside diphosphate kinase homolog 7

Macromolecule #5: Uncharacterized protein C1orf158 homolog

• Macromolecule: Name: Uncharacterized protein C1orf158 homolog / type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 23.271453 KDa

Sequence

String:

MQFLTAVSPQ SSSTPSWKIE TKYSTRVLTG NWTEERRKFI KATEKTPQTI YRKEYVPFPG HRPDQISRWY SKRTVEGLPY KYLITHHQE PSQRYLISTY DDHYNRHNYH PGLPELRTWN RHKLLWLPEK ADFPLLGPPT NYGLYEQLKQ KWLPPPEATL R ESIYTSSY PRPPAGAMSR REHAIPVPPP RLQPVPHF

UniProtKB: Cilia- and flagella-associated protein 107

Macromolecule #6: Meiosis-specific nuclear structural protein 1

• Macromolecule: Name: Meiosis-specific nuclear structural protein 1 / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 60.572148 KDa

Sequence

String:

MASIRRTLSF SERHQKLVDI NYCKKLHVEA LQRLQNQTRD QMVQNENDDR AERKRFLRLL QDEQFELDME EAIQKAEENK RLRELQLAQ EEKLATELAK LKRESLKDEK LRQQVRENSA ELRELEKKLK AAYMNKERAA QIAEKDAIKY EQMKRDAEIA R TMMEEHER LIKEESAAED KRNQAKAQYS HDLEKQLEEQ EKKKQEAYEQ LLKEKLMIDE IVRKIYEEDQ LERQQRLEKM NT TRRYIEE FQKEQALWRK KKREEMEEEN RKIIEFAKLQ QQREEDRMAK VQESEEKRLQ LKNMLTQRLE EMLRQREDLE QVR QELYQE EQAEIYKKKL EEEAEEKLRK QKELKQDFMD QMALKELILQ AAKEEEETFR KAMLAKFAED DRIELMNAQK QRMK QLEHK RAVEKLIEER RNQFLADKQR ELEEWQWQQR RQGCINAIVE EERLKLLKEH ATKLLGYLPK GVFKNEDDID MLGEE FRKA YQKRSEICEK

UniProtKB: Meiosis-specific nuclear structural protein 1

Macromolecule #7: Tektin-1

• Macromolecule: Name: Tektin-1 / type: protein_or_peptide / ID: 7 / Number of copies: 5 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 48.798375 KDa

Sequence

String:

MAKLLQQPPK FLRAEWQIAN KNQYHRAEAQ RSRSERLVAE SQRLVDEIEK TTRKSQSDVN KKLEQRLEEV RFWKKELDDK LEQLVYATE DLLLYQTRLQ KALESFKEPL HITEKCLEYR EKRVGIDLVH DEVEQELIKE HEIIRGVMTL LTRTLEETCE Q IRLNRSAK YNLEKDLRDK FTAITIDDIC FSLNNNSPNI KYSENVVRVE PNSVSLEDWL DFSNTNVEKA DKQRNNSLTL KA LVDRILF QTASDLRRQC DVVDTAFKNG LKETKDARDK LALHLDKVME EIASQEKNIV VLEKAILDQE GPAKVAHTRL ETR THRPNV ELCRDVAQYR LIKEVDEITH NVARLKETLA QAHVELKGLN RRQLALQEEI QIKENTIYID EVLCVPMRKS IPPR DGDDH GEWAGGSHPE AVC

UniProtKB: Tektin-1

Macromolecule #8: Tubulin alpha-1D chain

• Macromolecule: Name: Tubulin alpha-1D chain / type: protein_or_peptide / ID: 8 / Number of copies: 149 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 50.335594 KDa

Sequence

String:

MRECISVHVG QAGVQIGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDSFN TFFSETGAGK HVPRAVFVDL EPTVIDEVRT GTYRQLFHP EQLITGKEDA ANNYARGHYT IGKELIDLVL DRIRKLADQC TGLQGFLIFH SFGGGTGSGF TSLLMERLSV D YGKKSKLE FSIYPAPQVS TAVVEPYNSI LTTHTTLEHS DCAFMVDNEA IYDICRRNLD IERPTYTNLN RLIGQIVSSI TA SLRFDGA LNVDLTEFQT NLVPYPRIHF PLATYAPVIS AEKAYHEQLS VAEITNACFE PANQMVKCDP RHGKYMACCL LYR GDVVPK DVNAAIATIK TKRTIQFVDW CPTGFKVGIN YQPPTVVPGG DLAKVQRAVC MLSNTTAIAE AWARLDHKFD LMYA KRAFV HWYVGEGMEE GEFSEAREDM AALEKDYEEV GMDSVEGEGE EEEGDEY

UniProtKB: Tubulin alpha-1D chain

Macromolecule #9: Tubulin beta-4B chain

• Macromolecule: Name: Tubulin beta-4B chain / type: protein_or_peptide / ID: 9 / Number of copies: 153 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 49.877824 KDa

Sequence

String:

MREIVHLQAG QCGNQIGAKF WEVISDEHGI DPTGTYHGDS DLQLERINVY YNEATGGKYV PRAVLVDLEP GTMDSVRSGP FGQIFRPDN FVFGQSGAGN NWAKGHYTEG AELVDSVLDV VRKEAESCDC LQGFQLTHSL GGGTGSGMGT LLISKIREEY P DRIMNTFS VVPSPKVSDT VVEPYNATLS VHQLVENTDE TYCIDNEALY DICFRTLKLT TPTYGDLNHL VSATMSGVTT CL RFPGQLN ADLRKLAVNM VPFPRLHFFM PGFAPLTSRG SQQYRALTVP ELTQQMFDAK NMMAACDPRH GRYLTVAAVF RGR MSMKEV DEQMLNVQNK NSSYFVEWIP NNVKTAVCDI PPRGLKMSAT FIGNSTAIQE LFKRISEQFT AMFRRKAFLH WYTG EGMDE MEFTEAESNM NDLVSEYQQY QDATAEEEGE FEEEAEEEVA

UniProtKB: Tubulin beta-4B chain

Macromolecule #10: Tektin-2

• Macromolecule: Name: Tektin-2 / type: protein_or_peptide / ID: 10 / Number of copies: 10 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 49.962656 KDa

Sequence

String:

MATLSVKPSP RFRLPDWQTN SYLLSTNAER QRDASHQIRQ EARVLRNETN NQTIWDEHDN RTRLAERIDT VSRWKEMLDK CLTDLDAEI DALAQMKESA EQNLQAKNLP LDVAIECLTL RESRRDIDVV KDPVEEELHK EVEVIEATKK ALQQKISQAF E KLFLLQEA RQRLNSDHRG KMETLDIDRG CLSLNLTSPN ISLKINPTRV PNGSTSLQQW DDLSRFNKDH GEAEMKKAIE LR EAIALTI AETNNELEAQ RVATEFAFRK RLREMEKLYS ELKWQEKNTL EEIAELHEDI RHLEEDLRRK LQNLKLCHTR LEA RTYRPN VELCRDQAQY GLTDEVHQLE ATIAALKQKL AQAQDALDAL YKHLARLQAD IACKANSMLL DTKCMDTRRK LTVP AEKFV PEVDTFTRTT NRTLSPLKTC QLELA

UniProtKB: Tektin-2

Macromolecule #11: Uncharacterized protein C1orf189 homolog

• Macromolecule: Name: Uncharacterized protein C1orf189 homolog / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 12.361437 KDa

Sequence

String:

MSMEKMGKVE EHFQRALELK KMVHRWRNSH THCLWQITLS QRRNPYAILR MQDTMVQELA LANKQLLMVR QAALHQLFEK EHQQYQQEL NEKGKAFYME RL

UniProtKB: Cilia- and flagella-associated protein 141

Macromolecule #12: Tektin-3

• Macromolecule: Name: Tektin-3 / type: protein_or_peptide / ID: 12 / Number of copies: 15 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 56.76091 KDa

Sequence

String:

MELLGSTLTA TYAHPRPTPT NFLPAISTMA STYRDRFPHY NLTHSLSLPW RPSTYYKAAS NWPTLDPYCT RSQRVSESTM LPFVSNRTT LFTRYTPDDW YRSNLTNFQE SNTSRHNSER LRVDTSRLIQ DKYQQTRKTQ ADSTQNLGER VNDIGFWKSE I IHELDAMI GETNELTDIK KRLERALMET EAPLQVAREC LFHREKRMGI DLVHDEVEKE LLTEVDTILC CQERMKLYLD KA IAQLAAN RAAQHELEKD LSDKQSAYRI DDKCHHLRNT SDGVSYFHGV ERVDATVSVP ESWAKFTDDN ILRSQSERAA SAK LRDDIQ NVLVVTANEM WNQFNKVNLA FTNRIAETAD AKNKIQTHLA KTLQEIFQTE MTIESIKKAI VEKSAFLKVA QTRL DERTR RPNIELCRDM AQLRLVNEVY EVDDTIQTLQ QRLRDAEDTL QSLAHTKATL EHDLAVKANS LYIDQDKCMS MRRSF PSTL RLVGFC

UniProtKB: Tektin-3

Macromolecule #13: Sperm associated antigen 8

• Macromolecule: Name: Sperm associated antigen 8 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 51.604398 KDa

Sequence

String:

METSESTDRS QSRCLDLQPS SDGLGSSSDP FSSWDGRHRS ALVAATAAAS AAATAASTAR AAALWTKSPA PYSHGNLLTE PSSDSLTER YTGPRFTHKI SHGRLGFQPA YFSHIAWNPY TTNDLSSSRG PIPGSSSGPV PGSSSSPGPD SSSDPGPSSS S GPGGSPGG SGRGPGHGPG PGGGSGQGPG GGSGQGTDLG PAIDSRHSPG HGHGPRFNFS APVGFRNPRG DLIPNYTGCK HH CHWEPQK QSWKFLKVSE PGARGLWKPP EVEGKSTVLS ETLPRGQCLL YNWEEERATN YLDQVPVMQD GSESFFFRHG HRG LLTLQP QSPTSSCTTQ KDSYQPPKSH CQPIRGKREA ILEMLLRQQI CKEVQAEQEP TRKDSEVESV THHDYKKELV QAGP PAPTK IHDYHTEQPE TFWLERAPQL PGVSNIRTLD TPFRKNCSFS TPVPLSLEQP LPFEPESYSQ HGEISSLACQ GGGQG GGGG

UniProtKB: Sperm-associated antigen 8

Macromolecule #14: Tektin-4

• Macromolecule: Name: Tektin-4 / type: protein_or_peptide / ID: 14 / Number of copies: 9 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 52.025488 KDa

Sequence

String:

MAQTDILLTK EPAPQTVPAC QLPRKLYDVA RNTGAHTSSG LATSGFRTAK YLLDEWFQNC YARYHQAFAD RDQSERQRHE SQQLAAETE ALAQRTQQDS TRKVGERLQD MHGWKSELQR QVEELVSETE LLLAQKQRLE RALDATAGPF SIVTDNLQCR E RRQHPDLV RDCVEIELLK EAELIRNIQE LLKRTIKQAV SQIRLNWEHK ETCEMDWSDK VEAYNIDEAC CRYNNQSTDV QF YPHSAKF EESASTPETW AKFTQEHLYR AERERLASVN LRNLIDCILQ DTSEDLRLQC DAVNLAFGRR CEELEDARHK LEH HLRKTL REISDQEHNI AALKQAIKDK EAPLKVAQTR LYQRSHRPNV ELCRDAAQFR LASEVEELNL SLAALKEKLL EAEQ SLRNL EDTRMSLEKD IAIKTNSLFI DRHKCMAHRA HYPTVLQLAG YQ

UniProtKB: Tektin-4

Macromolecule #15: Cilia and flagella associated protein 161

• Macromolecule: Name: Cilia and flagella associated protein 161 / type: protein_or_peptide / ID: 15 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 36.519594 KDa

Sequence

String:

MAQNLYGPRV RIGNWNEDVY LEEEIMKDFL AKRDKGQLLI QRNRRLKENL LRPMQLSVSE DGYIHYGDKV MLVSPDHPET EADLFLPGD LSLCMTPDEI KAHLSNELEV PCGLSAAQTK IPVGRNTFTI LCAAGEVIGQ VLRYGQNFRL GITGGFDDRM L YLSSDHRT LLKSSKRSWL QEVFLTHEDS YLNCWQAAFP HPQLRLEYEG SPVPANTKIL ITHCHTNRGL VAHRHLFLRT YF GQEAEVA AHTYLDSHRV EKPKNHWMLV TGAPRKDLST MLDLPKPPAE DTRALEQERE QVSDPGARST PDARGCVPQC TLP M

UniProtKB: Cilia- and flagella-associated protein 161

Macromolecule #16: TEKTIP1

• Macromolecule: Name: TEKTIP1 / type: protein_or_peptide / ID: 16 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 24.533445 KDa

Sequence

String:

MQTLRRQAAW PCVPRGTLEV DFPPPLYSDD YLSQEGPRWT PAIKQATRWK YTPMGRDAAG QLWYTGLTNS DSREAWYTLP RAPDSPYRE AYARWHGCYG HRERSLPSAY TQRLRETAWY DPIIPAQYTD PSTRWGSVLW KDRPIRGKEF AINRHRFGVE A LWRASDYV RYLSAPQRPR YTAQNYRQWG LEPYCPATNQ RPPPVYTPSH

UniProtKB: Uncharacterized protein C19orf71 homolog

Macromolecule #17: Pierce2

• Macromolecule: Name: Pierce2 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 13.784531 KDa

Sequence

String:

MTECDWEKKS TSASNSDTEM KPELPPCVNP GNPVFSCMLD PKTLHTTTSL SKPKMIMYKT NSSNYGEFLP MPQFFPCYYT PREQVFSSH IRATGFYQNN TLNTAPDRTR TLDFPNFQHT L

UniProtKB: Piercer of microtubule wall 2 protein

Macromolecule #18: Protein FAM166B

• Macromolecule: Name: Protein FAM166B / type: protein_or_peptide / ID: 18 / Number of copies: 7 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 30.551627 KDa

Sequence

String:

MASTFIPGLN PQNPHYIPGY TGHCPLLRFS MGQTYGQMTG QLLRGSPGLA WPPAHRTLLP PIQPPRSPEP RRRSLPVRPG HERLSSSMV PGYTGFVPQA QFIFAKNCSQ VWAEALNGFT QRNGGQGSQE LPKEAKGEKD VEKDQEPKPE VEKEPELGQE A EQASPYSM DDRDPRKFFM SGFTGYVPRA RFLFGSSFPV LSNQALQEFG EMKSPGRSQK DPKHLPALSR TYPQHLGLLP KY GGYVPGY KFQFGRTYGH LTQDALGLST LQKQLLV

UniProtKB: Protein FAM166B

Macromolecule #19: Coiled-coil domain containing 114

• Macromolecule: Name: Coiled-coil domain containing 114 / type: protein_or_peptide / ID: 19 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 77.675969 KDa

Sequence

String:

MPFGLSAGST RSEDGSEAFL EGMVDWELSR LQRQCKVMED ERRAYSKEVH QRINKQLEEI QRLEGVRHKL RVQISIAQSQ VRRLRDSER LESMGHLLKC QVRVQAEVKE LQAQNQALDR EIQEWESRNS AHSKNARSPG CVQHDKVKSQ RRIKSLENQL D KVICRFDI QLAQNATLRE ELDLLRIERN RYLNVDRKLQ KEIQLLKDSV RNLMVSSTSA YTVREEAKAK LGMLRERAEK EV AQNETEV QILQRQIAHL EQLHHFLKLK NGDRQPDSAI VEKREQRARE VAEGLRKTSQ EKLVLRYEDA LNKLSQMTGE SDP DLLVEK YLELEERNFA EFNFINEQNS ELEHLQEEIK EMQEALVSGR RSEEDRRAQQ EQQRAELQQR VDDVHSEADD LEAR YHNFR EQLEKLKTNI QHLFTRAQCD STLINDLLGI KTHMRDRDIS LFLSLIEKRL VQLLTVQAFL ETQVVVMFNA ALMVL GQSS EDFPKKVAPP QPPDNLEDPP GFEAKDDYPL SKEELLSSVM KAEQHLKELV ESIKVESTPS MTSSTQKVSS SSRLVT QRP SQVPGSIMSH RTSGILVSSG GRATSSNVGH VTFGDSSATT GGLMSSRGSI PGRVTFRSPN SSSYLGSTGY VGSSRDH DS FEASKGPGSE SSGGLGSSPG PASSPGPASS TGQASSTSKD SQSNY

UniProtKB: Coiled-coil domain containing 114

Macromolecule #20: Outer dynein arm-docking complex subunit 3

• Macromolecule: Name: Outer dynein arm-docking complex subunit 3 / type: protein_or_peptide / ID: 20 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 72.163984 KDa

Sequence

String:

MTSPLCWAAA SNAMPSQDQI STPSKVKATQ VQLKPYRSRG KGLVPVWHSL HSKAGPLHAS EGKSAVNMQV AELQRKIQLL EGDRKAFYE STQWNIKKNQ ETINQLREET RVLQLQLTAL LQGDEKVVQA VIREWKSEKP YLKNRTGQQA LEHLDYRLNE K VKQLNALR HQLGLRQKWL EELQLQHSLR ELEIAEAQDS NTEVAKTMRN LENRLEKARM KAEEAEHITS VYLQLKAYLQ EE SLHLGNR LDFMEAEVVR TKHELEELHL VNQEALNARD IAKNQLQYLE ETVFRERKKR ERYLTECKKR AEEKKLQNER MER KTQREH VLLQSDDTLQ DSMYSKEEEL KRRWSMYQME VLFGKVKDAT GVAETHAVVR RFLAQGDTFT QLEMLKSENE QTLL RLKQE KQRLQQELED LKYSGEALLV SEQKRQAELQ GRLKMEEQRR ADAQNQLDRT MRALQITKEG LEHLAGKLNH IVVAG PTYE EGSPGASLDT KGSATPQPQE TGRSVGKMDP KVDDYLPNLL GLVEEKLLKL HSQLENHNVP EMLRHIVDLE FYATLE GKL PSYNTRIALP VAGHKDKFFD EEESEEDDSD VVTRAALKMR SQKLIESRSK RRGRSRRS

UniProtKB: Outer dynein arm-docking complex subunit 3

Macromolecule #21: Armadillo repeat containing 4

• Macromolecule: Name: Armadillo repeat containing 4 / type: protein_or_peptide / ID: 21 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 116.01707 KDa

Sequence

String:

MGVALTRSAQ WTAAGHGAKT LEVTPLNEAI VKEIIMFVES FIYKYPQEAN YVFVEPLEWK TNLDPSAFGS GYAVSGTTVK SEEADKNGE PLLYLSVPQI KIRSFGQLSR MLYIAKNMKL KEAQACIEAN RNPVAKILGL DYNIISEKIG NSFVSNILDK I TKDDDSES EIKMKIALQL KQLDLHLLNH SLKHISLEIR LNPGTVKNDI ELLKQFSGKG KQTVLESIEY TSDYEFSNGC RA PPWRQIK GEICYVLVKP HDAETLCVTC SKEGVFLNGG KTDDEGQINY ERKGEIYKDL VTLLKEKSAI FSENMSKQEI KFS EQPQKD QPNEAPKEEV AITHKASVTS RKSTQEKNRI NLGRSQLTKR LEPSLNWRTS VSSKDRNTLR DTQVEKHGGK LEKS RSSVS PGRAQLIRKS VEKIEEIISD SSSESEEDEE QPDHRQEANA DLPSEYWQIQ KLVKYLKGGN QTATVIALCS MKDFN LAQE TCQLAIRDVG GLEVLINLLE TDEVKCKIGS LKILKEISHN PQIRRNIVDL GGLPVMVNIL DSPHKSLKCL AAETIA NVA KFRRARRVVR RHGGITKLVA LLDCGKHSGE PAQSSLYETR DVEVARCGAL ALWSCSKSYA NKEAIRKAGG IPLLARL LK TSHENMLIPV VGTLQECASE ENYRAAIKAE RIIENLVKNL NSENEQLQEH CAMAIYQCAE DEETRDLVRL HGGLKPLA S LLNNTDNKER LAAVTGAIWK CSISKENVTK FREYKAIETL VGLLTDQPEE VLVNVVGALG ECCQEHENRV IIRRCGGIQ PLVNLLVGIN QALLVNVTKA VGACAVEPES MMIIDRLDGV RLLWSLLKNP HPDVKASAAW ALCPCIQNAK DAGEMVRSFV GGLELVVNL LKSDNKEVLA SVCAVITNIA KDQENLAVIT DHGVVPLLSK LANTNNDKLR RHLAETISRC CMWGRNRVAF G EHKAVAPL VRYLKSNDTN VHRATAQALY QLSEDADNCV TMHENGAVKL LLDMVGSPDE ELQEAAAGCI SNIRRLALAI EK ARYS

UniProtKB: Armadillo repeat containing 4

Macromolecule #22: TTC25 protein

• Macromolecule: Name: TTC25 protein / type: protein_or_peptide / ID: 22 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 78.492633 KDa

Sequence

String:

MADPENEVLR STFPSYMAEG ERLYLCGEFA KAAHSFSNAL HLQSGDKNCL VARSKCFLKM GELEKSLEDA EASLQGDPTF CKGILQKAE TLYTMGDFEF ALVFYHRGYK LRPDREFKVG IQKAQEAINN SVGSPSSIKL ENKGDLSFLS KQAESMRAQQ K PHPVRQLI HHPKRESKRK GSLKSEKIVR QLLGELYVDK EYLEKLLLDE DLIKGTIKHG LTVEDLIMTG INYLETRSDF WR QQKPIYA RERDRKLMQE KWLRDRKRRP SQTARYILKS LEDIDMLLTS GSAEGSLQKA EKVLKKVLEW NKEEVPNKDE LVG NLYSCI GNAQIELGQM VAALQSHRKD LEIAKEYDLP DAKSRALDNI GRVFARVGKF QQAIDTWEEK IPLAKTTLEK TWLF HEIGR CYLELDQAWE AQSYGEKSQQ CAEEEGDMEW QLNASVLVAQ AQVKLRDFES AVNNFEKALE RAKLVHNNEA QQAII NALD DANKGIIEEL KKTNYREILK EKKEKENATM LDGQTRTAKE KETRKTKDEP EKVMKQWVQE QTEKQLEGVL SKETLG VTA RQPEQRQRED PEKASWRKEL GAKERGPGDT AKGQFGEAGR TEQNREETRE IYRRPSELDQ NLSDESSPRE SEGLEKR LS KTDGGELEAL GKTESGEIKE MEITENSEKI EKDEKEDEPI E

UniProtKB: TTC25 protein

Macromolecule #23: EF-hand calcium-binding domain-containing protein 1

• Macromolecule: Name: EF-hand calcium-binding domain-containing protein 1 / type: protein_or_peptide / ID: 23 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 24.667131 KDa

Sequence

String:

MNRKKLQKLT DSLTKNCKHF SKFEVKCLIN LFYNLVGEVT ERQGVIIGLD RNAFRNILHM TFGMTDDMIM DRVFRGFDKD NDGCISVTE WVYGLSVFLR GTLEEKMKYC FEVFDLNGDS FISKEEMFHM LKNSLLKQPS EEDPDEGIKD LVEITLKKMD H DHDGKLSF ADYEQAVREE TLLLEAFGPC LPDPKSQSEF EAQVFKDPNE FNEV

UniProtKB: EF-hand calcium-binding domain-containing protein 1

Macromolecule #24: RIB43A-like with coiled-coils protein 2

• Macromolecule: Name: RIB43A-like with coiled-coils protein 2 / type: protein_or_peptide / ID: 24 / Number of copies: 5 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 44.773418 KDa

Sequence

String:

MEVAQPKDLK EDFVLAKRRH AELVRQKRIF NARNRIIGGD TTAWDAQVCD QNIKAATEKA RDEAFAAEMR QNDKIACLSE NRERRDRKN LCKAINDFQQ SFQRPETRRE FDLSDPLALK KDRPARQSDY DARNTISGMQ KFMGEDLNFH LRKKFQEEQN R EWSLQQQK EQMIGRENQK CAEDLYLKTR LQFDETAKHL QNLETATRKA VCATVKEFNK NQALESAEKK IQERKQEQED NL AEISNML RGDLLSENPQ QAASSFGPHR VVPDRWKGMS QEQLEEIRLV QRQQVQEKLR LQEEERQRDM DWDRRRIQKA RAT LLFEQQ QQRLQRGLRR ALDCSNLSLA REQLLQKKHM KELCTNHATE DYFTQFNTGS R

UniProtKB: RIB43A-like with coiled-coils protein 2

Macromolecule #25: EF-hand domain containing 1

• Macromolecule: Name: EF-hand domain containing 1 / type: protein_or_peptide / ID: 25 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 74.125344 KDa

Sequence

String:

MVSNPVHGLP FLPGTSFKDL TKTAFHRSQT LGYRNGYAVV RRPTVGIGGD RLQVNQLSQA DLDELASKIP ILTYGQARQA PPAAFVPAH VAFDKKVLKF DAYFQEDVPM SIEEHYRIRQ VHIYYYLEDD SMSVIEPVVE NSGIPQGKLI KRQRLSKNDR G DHYHWKDL NRGINITIYG KTFRIVDCDK FTQVFLESQG IELNPPEKMA LDPYTELRKQ PLRKYVTPTD FDQLKQFLTF DK QVLRFYA IWDDTDSMFG ECRTYIIHYY LMDDTVEIRE VHERNDGRDP FPLLMNRQRM PKVLVENAKN FPRCVLEISD KEV LEWYTA KDFIVGKPLT ILGRTFFIYD CDPFTRQYYQ EKFGISDLPR IDMSKKEPPP MKQELPPYNG FGLIEDSAQN CFAL IPKAP QKDVIKMLMN ENKVLRYLAT LESPFPEDKG RRFVLSYFLA TDMISIFEPP VRNSGIIGGK YLGRTKVVKP GSSVE NPVY YGPSDFFIGA VIEVFGHRFV ILDTDDYVLK YMESNAAQYS PEALLSIQNH IRKQEAPAPE LDGQQAEEDP GVRDLE ALI DTIQKQLKDR PCRDNIREAF QIYDKEASGY VDRETFFKIC GSYQLPVDDS LIKELIRMCS HGEDKIDYYN FVRAFSN

UniProtKB: EF-hand domain-containing protein 1

Macromolecule #26: EFHC2

• Macromolecule: Name: EFHC2 / type: protein_or_peptide / ID: 26 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 85.566766 KDa

Sequence

String:

MGKEKFHKSQ HWGFCNNVRM LVGEDKPGIG GELLFGQKIK PKYSVFPKGM GTDSPSWVAF DKQVLSFDAY LEDEVPDKSQ ENYRIRRYK IYFYLEDDTV EVNEPVLQNS GLPQGIFIRR HRISLPPPNE DQFYTVHHFN VNTDIVFYGR TFKVYDCDAF T KNFLTKIG VKLNPPGQCP EDPYMKTRRE KLDCMEPICP YESFDTLKQF LEYDRKVLRF FCVWDDSGSV FGDRRELILH YF LSDDTIE IKEVLPHNSG RDAMSLFLQR RKLPKYGPPG VYQPGQLTDQ TVLNVYGGYS ETRVYGFLLD KYQLGKLDQE FYK DTDLSI GTTINVWGRK VLLCDCDDFT KTYYRTKYGI ENFTSIPCKA PSPPKIERKF PPYTGFGSEE DSLRSCIGLM PTPH QRNFK KFIEFDSYGN ISNTLRFFAK LITHKCADVE RMFVISYFLS DDTISVFEPI ERNSGYTGGM FLKRVRVKKP GQEVF KSEF SEYIKAEELY VGAKVNVNGY LFFLVNADEY TLNYMERNSD KFPLSSIELV IQKLKEEECK SRELKQVFTA ADCMHT KMV DFNTFREIMM NLTVGKLTDQ EVITIARRYR VPEDPCPHRN VLVAQAHEQL KKNAFENFER LIAMCVYEDR EKKKVLP SK DIKRLCKSSR LPLNEDLLGS LLSGFEDSEK QINYESFFCA LNWRVNPVPV LEVASYIKER CEDEWLGMPS PIPVKYIY Y LNLLKDVFGV DE

UniProtKB: EF-hand domain-containing family member C2

Macromolecule #27: Cilia- and flagella-associated protein 20

• Macromolecule: Name: Cilia- and flagella-associated protein 20 / type: protein_or_peptide / ID: 27 / Number of copies: 7 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 22.781389 KDa

Sequence

String:

MFKNTFQSGF LSILYSIGSK PLQIWDKKVR NGHIKRITDN DIQSLVLEIE GTNVSTTYIT CPADPKKTLG IKLPFLVMII KNLKKYFTF EVQVLDDKNV RRRFRASNYQ STTRVKPFIC TMPMRLDDGW NQIQFNLSDF TRRAYGTNYI ETLRVQIHAN C RIRRVYFS DRLYSEDELP AEFKLYLPVQ NKAKQ

UniProtKB: Cilia- and flagella-associated protein 20

Macromolecule #28: PACRG protein

• Macromolecule: Name: PACRG protein / type: protein_or_peptide / ID: 28 / Number of copies: 6 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 29.323059 KDa

Sequence

String:

MVAEKETLSL NKCPAKLPTR TKLLAQQPLP VHQPHSLVSE GFTVKAMMKN SVVRGPPSAG AFKERPTKPT AFRKFYERGD FPIALEHDS KGNKIAWKVE IEKLDYHHYL PLFFDGLCEM TFPYEFFARQ GIHDMLEHGG NKILPVIPQL IIPIKNALNL R NRQVICVT LKVLQHLVVS AEMVGEALVP YYRQILPILN IFKNKNVNSG DGIDYSQQKR ENIGDLIQET LEAFERYGGE DA FINIKYM VPTYESCLLN

UniProtKB: Parkin coregulated gene protein

Macromolecule #29: Cilia and flagella associated protein 45

• Macromolecule: Name: Cilia and flagella associated protein 45 / type: protein_or_peptide / ID: 29 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 65.800328 KDa

Sequence

String:

MPLSTAGVLS SASTASNRSR NRPRYRTKAL SSEVDESLFG AIKPLTRSDS PIVLLRDKHA IRKTLTALGL DHKPETIQLI TRDMVRELI IPTKDPSGQS LIMSPEEFER IKWASHVLTK EELEAREQAF KKEKEAIVDT VTTRKKIMKQ KEMVWRNNRK L SDLEEVAK ERAQNLLQRA NQLRMEQEEE LKDMKKIILN AKCHAIRDAQ ILEKQLIQKE LDAEEKRLDQ MMEVERQKSV QR QEELDRK RREERIRGRR HIVEQMEKNQ EERSLLAEQR EQEKEQMLEY MEKLQEEDLR DLEQRHQQKL KMQAEIKRIN DEN QRQKAE LLAQEKLADQ MVMEFTKKKM AREAEFEAEQ ERIRREKEKE IARLRAMQEK AQDYQAEQDA LRAKRNQEVA DREW RRKEK ENAQKKMETE AKLRKSRLEQ VAFKEHTLAV QVQRDRDEFE RILRAQREQI EKERLEEEKK ATGRLQHANE LRRQV RENQ QKQVQARIAT FDEGRRLKEE AQKRRERIED IKRKKLEELR ATGLPEKYCI EAERKANIPV NTSVN

UniProtKB: Cilia- and flagella-associated protein 45

Macromolecule #30: Cilia and flagella associated protein 52

• Macromolecule: Name: Cilia and flagella associated protein 52 / type: protein_or_peptide / ID: 30 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 68.719602 KDa

Sequence

String:

METQASPKDE VAEAVELELE AVIGFNGHVP TGLKCHPDQE HLVYPLGCTI LIQAINTQEQ NFLHGHGNNV SCVAISKSGL YIASGQVTF MGFKADIILW DYKKRELMAR LSLHKGKIEA LAFSPNDMYL VSLGGPDDGS VVVWSIAKRE AICGSPAAGL N VGNATTVI FSKCRDEMFV TAGNGTIRVW ELDLPNRKIW PTECQTGQMK RIVMSISMAN DDSFFYLGTT TGDILKMNPR TK LLADTGP AKDKFSLGVS AICCLKMGGL LVGSGDGLLV FCKSPSYKPI KKIQLQGGIT SITLRGEGHQ FFVGTEESHI YRV NFTNFK ETLITTCHFE SVEDIVFPFG TAELFATCAK KDIRVWHTLT NRELLRITVP NMTCHGIDFM RDGKSIISAW DDGR IRAFA PETGRLMYVI NNAHRIGVTA IATTSDCKRV ISGGGEGEVR VWHIGHQTQK LEEALKEHKS SVSCIRVKKS NEECV TAST DGTCIIWDLV RLRRNQMILA NTLFQCVCYH PEEFQIITSG TDRKIAYWEV FDGSVIRELD GSLSGAVNGM DITVEG VHF VTGGNDHLVK VWDYNEGEVT HVGVGHSGNI TRIRISPGNQ YIVSVSADGA ILRWKYPFPS

UniProtKB: Cilia- and flagella-associated protein 52

Macromolecule #31: Enkurin, TRPC channel interacting protein

• Macromolecule: Name: Enkurin, TRPC channel interacting protein / type: protein_or_peptide / ID: 31 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 30.215129 KDa

Sequence

String:

MVAMDPTGPS ESIYNLIPSD WKEPPQPPRY VSIFKATVKD DMQKFKTAMK TMGPAKLEVP SPKDFLKKHS KEKILPPKKK FEWNERRKP PVPLRTDHPV MGIQSEKNFI NTNAADVIMG VAKKPKPIYV DKRTGDKHDL ETSGLVPKYI NKKDYGVTPE Y ICKRNEEV KKAQEEYDNY IQENLRKAAM KRLSDEEREA VLQGLKKNWE EVHKEFQSLS VFIDSIPKKI RKQKLEEEMK QL EHDIGVL EKHKVIYIAN KK

UniProtKB: Enkurin

Macromolecule #32: Protein Flattop

• Macromolecule: Name: Protein Flattop / type: protein_or_peptide / ID: 32 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 21.214035 KDa

Sequence

String:

MATNYSANQY EKPFSPKYLQ NWSLAKPTKE RISSHEGYTQ IIANDRGHLL PSVPRSKASP WGSFMGTWQM PLKVPPARAT LTSRTAAGA ASLTRWIQKN PDLLKASNGL RPEIFGKPHD PDSQKKLRKS ITKTVQQAPS PTIIPSSPAS NLSSPDQLQS S HPSAGHTP GPQSPLNSPK CPPGSPCLPH AGRNLAEV

UniProtKB: Protein Flattop

Macromolecule #33: Coiled-coil domain containing 173

• Macromolecule: Name: Coiled-coil domain containing 173 / type: protein_or_peptide / ID: 33 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 65.437598 KDa

Sequence

String:

MLVRFGRRSG QAKESAEMKN CEEDPVLYPP LLPTKVDLRQ VTIIPHNEWE RIRDSLDSLT REAACLRAER KAKKEMHLRS QEVVKHWTN TYAGMKEQKL EAKKKRDEEI EAERQILDIE EAIYKQGERK KAIELAKQYQ FYQTERVKNF HSGLLLSRVM K ERDAQIEF QKSKIKSDKK WEEQVKLNVE KAFKEEREKA EKQRRERVAL AKDHLKQIKE HEEEEERRRK EEEKDAEEIK RQ NSLYEIE MKKKQGKKKE EINESRRLFF EHLNDKHIIK AVEQQQQEEE DEKIRKFIKA KKRLTQMGKE KEAETHRLME ERR KRINNF LSKLMKEKFD NEDLIIARDI AEAEAEWEKR EREKYEKNKA ELKAIAEHRA LVMKNKEEEE RQRKIEATEQ MLAI LKADQ IFWEHEKEKK QKADKERREV QDAHIQQMAK HKFNALQAKQ AESEYCRLTE ALVAEKEKEF QDYAREVIES ESEST KKYI YPLVKAVQEG PGGGRGPVLV DRGGLRPSYQ ANDTTGVQLP FYNSPGSKYN NFQKSKGRLG FTW

UniProtKB: Cilia- and flagella- associated protein 210

Macromolecule #34: Chromosome 3 C1orf194 homolog

• Macromolecule: Name: Chromosome 3 C1orf194 homolog / type: protein_or_peptide / ID: 34 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 19.387805 KDa

Sequence

String:

MPLTRDPFQN PALDKDDSYL GKSRASKKLP YKNPTHLAQQ QEPWCRLSST PTITSMKRDG FFFYSEIPKD DLDFRLAALY NHHTGTFKN KSEILTHQET IQDTRRIKTQ FPGEFLPAPQ PPLITSRANI RHWINPKKES IHSIQGSIVS PHTAATNGGY S RKNDGGFF ST

UniProtKB: Cilia- and flagella-associated protein 276

Macromolecule #35: EFCAB6

• Macromolecule: Name: EFCAB6 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 172.993375 KDa

Sequence

String:

MCKMAVVPDW HIICPYTRKF THSRPHSSPC RVYSRSVFQN MFRPSSSTTV ANPILSSLDV KRILFQKITE KGDELKRAFH LLDTANNMT VTKSELRRVI TTFLLPLTRE QFQDVLAQIP LTSSGAVPYL VFLSRFGGID LNINVIKRGG ENEMNGCRTL K DLEAQVGE KIFKNIKTVI KALMLIDVNT TGLVQPHELR RVLETFCLKM KDEEYKKFAQ HYNIDKDAAV DYNVFLKNLG SN NDLNLKY NMGSQEVLWE NQRVKSFKRE EPPSSAVSDN IWKNYSLDEI ETAFCLELSK CYEKVEKALS AGDPCTSGYV SLN YLKIVL DTFVYRLPRR IFIQLMKRFG LKTTTKVNWK QFLTSFYEPQ YEASSKAPLT KRNSINFRNQ VHKENVITKL FRHE DHDSS LKKALLLIKS KPDGQITGQE LQRILNCMVV KISDSEFREL MRILDPGCTG CVNVSRFIEL IEESPKLHKI PAYKD TKMP LFLAWDSVEE IIHDSIAKNL SAFCNMLRSY DLGDTGLIGR NNFKKIMRVF CPFLTTEHLV KLCSKFQDIA SGRILY KKL LACLGINRPP TVSPVSMPKG QLLNEHLQKE EQPLPDHSER ATPAESQGAV TRNMTKDEVI EKLKSSIRQQ DPAFRKR FL DFTKEPNGKI HTHDFKKVLE DYGMPMDDDQ YALLTTKLGY KKEGMSYLDF AAGFEDPKMN EPEVTPPQTP VLPKGDVD S FFVTAEECLR QFPRRLKEFF RDPYAAFFKM DTDRDGVLTM HDLHRLLQHL LFNLKDEEFE RLLGLLGLRL SITLNFREF RNLCEKRSFS ADDDAPQRLP RPKQKVADSE LACEQAHQYL VTKAKTRWSD LSKNFIETDS EGNGILRRRD MKNALYGFDI PLTPREFEK LWMRYDSEGR GHITYQEFLQ KLGINYSADI HRPYAEEYFN FMGHFTKPQQ VQEELKELQQ STEKAMPARD K LKDHDQDI SKALAKLDKS RTGYISLGRL QRLLQECGCS LKEEELIDLL NSWGIAWHNN SINYLDFLRA VENNKLTRPQ SQ EKEESVP INFAALSPEE VLKNIQGVIA ASSLALSTAF SALDKEDTGF VKASDFGQVL KDFCYKLTDN QYHYFLRKLR LHL TPYINW KYFLQNFSSY VEETAVEWAE KMPRGPRPLS PKEMANQELL ARLHKAVTSH YHAIAQEFEN FDTMKTNTAS RDEF RSICT RHVQVLTDEQ FDRLWEEMPV NSKGRLRYLD FLSSFNVDKQ ATPLATSDSA KAQRGSSVPE VSDRGRSAGS SPTRD PKAG SKPRSHPCTP SSVSGTPPLQ NCEPIETKLR KKVQGCWREL LRECKERDFN KQGEIPGPEF LALVEKFNLD ISRDEC QQL LIKYDLKNNG KFAYCSFIQS CVLLLKAKET SLMQRMKIQN ANKMKEAGAE TCSFYSALLR IQPKIVHCWR PMRRTFK AY DEGGTGLLSV ADFRKVLRQY SINLSEEEFF HILEYYDKTL SSKISYNDFL RAFLQ

Macromolecule #36: Pierce1

• Macromolecule: Name: Pierce1 / type: protein_or_peptide / ID: 36 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 15.673458 KDa

Sequence

String:

MSEEDPKACA EPEEPKAGPP PEKTSDWYRV SEDLPARFNN PAWFRGYRTK EPPSVYRTSN QAYGSRAPTV HEMPKVFYPN SYKFSRQVA VGGMFQNNTL NVYMEKSIVT GPDNYITSYD RLNFHPSYRV CRPSICD

UniProtKB: Piercer of microtubule wall 1 protein

Macromolecule #37: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 37 / Number of copies: 149 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Macromolecule #38: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 38 / Number of copies: 149 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #39: GUANOSINE-5'-DIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 39 / Number of copies: 153 / Formula: GDP
• Molecular weight: Theoretical: 443.201 Da

Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: filament

Sample preparation

• Buffer: pH: 7.4
• Grid: Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec. / Details: unspecified
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 60.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 1267170

Startup model

Type of model: EMDB MAP
EMDB ID:

20631

• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1) / Number images used: 80503
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)

Atomic model buiding 1

• Refinement: Protocol: AB INITIO MODEL

Output model

PDB-7rro:
Structure of the 48-nm repeat doublet microtubule from bovine tracheal cilia