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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 7rro | ||||||
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タイトル | Structure of the 48-nm repeat doublet microtubule from bovine tracheal cilia | ||||||
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![]() | STRUCTURAL PROTEIN / cilia / microtubule / dynein / motility | ||||||
機能・相同性 | ![]() outer dynein arm docking complex / protein localization to motile cilium / regulation of cilium movement / outer acrosomal membrane / epithelial cilium movement involved in determination of left/right asymmetry / regulation of brood size / establishment of left/right asymmetry / 9+0 motile cilium / mucociliary clearance / sperm flagellum assembly ...outer dynein arm docking complex / protein localization to motile cilium / regulation of cilium movement / outer acrosomal membrane / epithelial cilium movement involved in determination of left/right asymmetry / regulation of brood size / establishment of left/right asymmetry / 9+0 motile cilium / mucociliary clearance / sperm flagellum assembly / manchette assembly / axonemal B tubule inner sheath / axonemal A tubule inner sheath / regulation of flagellated sperm motility / protein polyglutamylation / inner dynein arm assembly / regulation of calcineurin-NFAT signaling cascade / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Cilium Assembly / Intraflagellar transport / Carboxyterminal post-translational modifications of tubulin / Sealing of the nuclear envelope (NE) by ESCRT-III / Kinesins / positive regulation of feeding behavior / Resolution of Sister Chromatid Cohesion / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / outer dynein arm assembly / cilium-dependent cell motility / COPI-dependent Golgi-to-ER retrograde traffic / COPI-independent Golgi-to-ER retrograde traffic / COPI-mediated anterograde transport / 転移酵素; リンを含む基を移すもの / sperm principal piece / RHO GTPases activate IQGAPs / cerebrospinal fluid circulation / regulation of cilium beat frequency involved in ciliary motility / RHO GTPases Activate Formins / cilium movement involved in cell motility / regulation of store-operated calcium entry / 9+2 motile cilium / MHC class II antigen presentation / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / acrosomal membrane / regulation of cilium assembly / axoneme assembly / cilium movement / ciliary transition zone / Aggrephagy / calcium ion sensor activity / axonemal microtubule / The role of GTSE1 in G2/M progression after G2 checkpoint / Separation of Sister Chromatids / cilium organization / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / Anchoring of the basal body to the plasma membrane / AURKA Activation by TPX2 / Recruitment of NuMA to mitotic centrosomes / ciliary tip / gamma-tubulin ring complex / ciliary rootlet / manchette / Regulation of PLK1 Activity at G2/M Transition / Hedgehog 'off' state / flagellated sperm motility / UTP biosynthetic process / CTP biosynthetic process / determination of left/right symmetry / Neutrophil degranulation / positive regulation of cell motility / motile cilium / nucleoside diphosphate kinase activity / GTP biosynthetic process / AMP binding / regulation of neuron projection development / cerebral cortex cell migration / microtubule organizing center / mitotic cytokinesis / regulation of cell division / cellular response to UV-C / axoneme / cilium assembly / glial cell projection / spermatid development / alpha-tubulin binding / regulation of signal transduction / beta-tubulin binding / single fertilization / sperm flagellum / intercellular bridge / sperm midpiece / Hsp70 protein binding / 3'-5' exonuclease activity / centriole / acrosomal vesicle / lung development / mitotic spindle organization / meiotic cell cycle 類似検索 - 分子機能 | ||||||
生物種 | ![]() ![]() | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.4 Å | ||||||
![]() | Gui, M. / Anderson, J.R. / Botsch, J.J. / Meleppattu, S. / Singh, S.K. / Zhang, Q. / Brown, A. | ||||||
資金援助 | ![]()
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![]() | ![]() タイトル: De novo identification of mammalian ciliary motility proteins using cryo-EM. 著者: Miao Gui / Hannah Farley / Priyanka Anujan / Jacob R Anderson / Dale W Maxwell / Jonathan B Whitchurch / J Josephine Botsch / Tao Qiu / Shimi Meleppattu / Sandeep K Singh / Qi Zhang / James ...著者: Miao Gui / Hannah Farley / Priyanka Anujan / Jacob R Anderson / Dale W Maxwell / Jonathan B Whitchurch / J Josephine Botsch / Tao Qiu / Shimi Meleppattu / Sandeep K Singh / Qi Zhang / James Thompson / Jane S Lucas / Colin D Bingle / Dominic P Norris / Sudipto Roy / Alan Brown / ![]() ![]() ![]() 要旨: Dynein-decorated doublet microtubules (DMTs) are critical components of the oscillatory molecular machine of cilia, the axoneme, and have luminal surfaces patterned periodically by microtubule inner ...Dynein-decorated doublet microtubules (DMTs) are critical components of the oscillatory molecular machine of cilia, the axoneme, and have luminal surfaces patterned periodically by microtubule inner proteins (MIPs). Here we present an atomic model of the 48-nm repeat of a mammalian DMT, derived from a cryoelectron microscopy (cryo-EM) map of the complex isolated from bovine respiratory cilia. The structure uncovers principles of doublet microtubule organization and features specific to vertebrate cilia, including previously unknown MIPs, a luminal bundle of tektin filaments, and a pentameric dynein-docking complex. We identify a mechanism for bridging 48- to 24-nm periodicity across the microtubule wall and show that loss of the proteins involved causes defective ciliary motility and laterality abnormalities in zebrafish and mice. Our structure identifies candidate genes for diagnosis of ciliopathies and provides a framework to understand their functions in driving ciliary motility. | ||||||
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構造の表示
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構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 26.7 MB | 表示 | ![]() |
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PDB形式 | ![]() | 表示 | ![]() | |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 21.5 MB | 表示 | ![]() |
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文書・詳細版 | ![]() | 23.4 MB | 表示 | |
XML形式データ | ![]() | 3.5 MB | 表示 | |
CIF形式データ | ![]() | 5.4 MB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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要素
+タンパク質 , 27種, 407分子 073456ABA0A1A2A3A4AAACAEAGAIAKAMBABCBEBGBIBKBMCACCCE...
-EF-hand domain ... , 2種, 5分子 12TUV
#2: タンパク質 | 分子量: 97960.547 Da / 分子数: 2 / 由来タイプ: 天然 / 由来: (天然) ![]() ![]() #25: タンパク質 | 分子量: 74125.344 Da / 分子数: 3 / 由来タイプ: 天然 / 由来: (天然) ![]() ![]() |
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-Uncharacterized protein ... , 2種, 3分子 89C
#5: タンパク質 | 分子量: 23271.453 Da / 分子数: 2 / 由来タイプ: 天然 / 由来: (天然) ![]() ![]() #11: タンパク質 | | 分子量: 12361.437 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) ![]() ![]() |
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-Cilia and flagella associated protein ... , 3種, 9分子 EFabcdefg
#15: タンパク質 | 分子量: 36519.594 Da / 分子数: 2 / 由来タイプ: 天然 / 由来: (天然) ![]() ![]() #29: タンパク質 | 分子量: 65800.328 Da / 分子数: 4 / 由来タイプ: 天然 / 由来: (天然) ![]() ![]() #30: タンパク質 | 分子量: 68719.602 Da / 分子数: 3 / 由来タイプ: 天然 / 由来: (天然) ![]() ![]() |
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-Coiled-coil domain containing ... , 2種, 5分子 H1H2H3op
#19: タンパク質 | 分子量: 77675.969 Da / 分子数: 3 / 由来タイプ: 天然 / 由来: (天然) ![]() ![]() #33: タンパク質 | 分子量: 65437.598 Da / 分子数: 2 / 由来タイプ: 天然 / 由来: (天然) ![]() ![]() |
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-非ポリマー , 3種, 451分子 




#37: 化合物 | ChemComp-GTP / #38: 化合物 | ChemComp-MG / #39: 化合物 | ChemComp-GDP / |
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-詳細
研究の焦点であるリガンドがあるか | N |
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-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: FILAMENT / 3次元再構成法: 単粒子再構成法 |
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試料調製
構成要素 | 名称: Doublet microtubule / タイプ: COMPLEX / Entity ID: #1-#36 / 由来: NATURAL |
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分子量 | 実験値: NO |
由来(天然) | 生物種: ![]() ![]() |
緩衝液 | pH: 7.4 |
試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
試料支持 | 詳細: unspecified |
急速凍結 | 凍結剤: ETHANE |
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電子顕微鏡撮影
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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顕微鏡 | モデル: FEI TITAN KRIOS |
電子銃 | 電子線源: ![]() |
電子レンズ | モード: BRIGHT FIELD / アライメント法: COMA FREE |
試料ホルダ | 凍結剤: NITROGEN 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER |
撮影 | 電子線照射量: 60 e/Å2 フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k) |
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解析
EMソフトウェア |
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CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||||||
粒子像の選択 | 選択した粒子像数: 1267170 | ||||||||||||||||||||||||||||
3次元再構成 | 解像度: 3.4 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 80503 / 対称性のタイプ: POINT | ||||||||||||||||||||||||||||
原子モデル構築 | プロトコル: AB INITIO MODEL |