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- EMDB-20631: Composite cryo-EM density map of the 48-nm repeat doublet microtu... -

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Basic information

Entry
Database: EMDB / ID: EMD-20631
TitleComposite cryo-EM density map of the 48-nm repeat doublet microtubule from Chlamydomonas reinhardtii
Map dataComposite cryo-EM density map of the 48-nm repeat doublet microtubule from Chlamydomonas reinhardtii
Sample
  • Complex: Doublet microtubule from wild-type Chlamydomonas reinhardtii
    • Protein or peptide: x 38 types
  • Ligand: x 3 types
Keywordsmotile cilia / microtubule doublet / repetitive structure / PROTEIN FIBRIL
Function / homology
Function and homology information


axonemal central pair / axonemal doublet microtubule / positive regulation of cilium-dependent cell motility / outer dynein arm / cilium-dependent cell motility / outer dynein arm assembly / regulation of cilium beat frequency involved in ciliary motility / cilium movement involved in cell motility / establishment of protein localization to organelle / cilium movement ...axonemal central pair / axonemal doublet microtubule / positive regulation of cilium-dependent cell motility / outer dynein arm / cilium-dependent cell motility / outer dynein arm assembly / regulation of cilium beat frequency involved in ciliary motility / cilium movement involved in cell motility / establishment of protein localization to organelle / cilium movement / axoneme assembly / axonemal microtubule / negative regulation of microtubule depolymerization / nucleoside-diphosphate kinase / UTP biosynthetic process / CTP biosynthetic process / microtubule associated complex / motile cilium / nucleoside diphosphate kinase activity / GTP biosynthetic process / mitotic cytokinesis / axoneme / cilium assembly / alpha-tubulin binding / microtubule-based process / Hsp70 protein binding / mitotic spindle organization / ciliary basal body / meiotic cell cycle / Hsp90 protein binding / ATP-dependent protein folding chaperone / structural constituent of cytoskeleton / cilium / mitotic spindle / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / microtubule / cytoskeleton / calmodulin binding / hydrolase activity / GTPase activity / calcium ion binding / GTP binding / ATP binding / nucleus / metal ion binding / cytoplasm
Similarity search - Function
: / : / ODAD1 central coiled coil region / Protein Flattop / Flattop / Nucleoside diphosphate kinase 7 / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 141 / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 141 ...: / : / ODAD1 central coiled coil region / Protein Flattop / Flattop / Nucleoside diphosphate kinase 7 / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 141 / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 141 / : / : / Meiosis-specific nuclear structural protein 1 / Cilia- and flagella-associated protein 45 / NDPK7, second NDPk domain / Cilia- and flagella- associated protein 210 / CFAP53/TCHP / Trichohyalin-plectin-homology domain / Trichohyalin-plectin-homology domain / RIB43A / RIB43A / DM10 domain / EF-hand domain-containing protein EFHC1/EFHC2/EFHB / DM10 domain / DM10 domain profile. / Domains in hypothetical proteins in Drosophila, C. elegans and mammals. Occurs singly in some nucleoside diphosphate kinases. / Enkurin domain / : / Calmodulin-binding / Enkurin domain profile. / : / CFA20 domain / Cilia- and flagella-associated protein 20/CFAP20DC / CFA20 domain / : / Parkin co-regulated protein / Parkin co-regulated protein / EF-hand domain / Nucleoside diphosphate kinase, active site / Nucleoside diphosphate kinase (NDPK) active site signature. / Nucleoside diphosphate kinase / Nucleoside diphosphate kinase-like domain / Nucleoside diphosphate kinase / NDK / Nucleoside diphosphate kinase-like domain superfamily / Heat shock protein 70kD, peptide-binding domain superfamily / Heat shock protein 70 family / Hsp70 protein / Alpha tubulin / Tubulin-beta mRNA autoregulation signal. / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Nucleotide-diphospho-sugar transferases / Tubulin/FtsZ, GTPase domain superfamily / EF-hand domain pair / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / Armadillo-type fold / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily
Similarity search - Domain/homology
Meiosis-specific nuclear structural protein 1 / Cilia- and flagella-associated protein 52 / Flagellar associated protein / Flagellar associated protein / Flagellar associated protein / Flagellar associated protein / Trichohyalin-plectin-homology domain-containing protein / Flagellar associated protein / Uncharacterized protein / Uncharacterized protein ...Meiosis-specific nuclear structural protein 1 / Cilia- and flagella-associated protein 52 / Flagellar associated protein / Flagellar associated protein / Flagellar associated protein / Flagellar associated protein / Trichohyalin-plectin-homology domain-containing protein / Flagellar associated protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / EF-hand domain-containing protein / Cilia- and flagella-associated protein 45 / Uncharacterized protein / Flagellar associated protein / Uncharacterized protein / Cilia- and flagella-associated protein 53 / Cilia- and flagella-associated protein 20 / Protein Flattop homolog / Uncharacterized protein / Flagellar associated protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Outer dynein arm protein 1 / Nucleoside diphosphate kinase / Uncharacterized protein / Parkin-co-regulated gene product / Tubulin beta-1/beta-2 chain / Tubulin alpha-1 chain / Outer dynein arm-docking complex protein DC3 / RIB72 protein / Flagellar protofilament ribbon protein
Similarity search - Component
Biological speciesChlamydomonas reinhardtii (plant)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.4 Å
AuthorsMa M / Stoyanova M / Rademacher G / Dutcher SK / Brown A / Zhang R
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01GM032843 United States
CitationJournal: Cell / Year: 2019
Title: Structure of the Decorated Ciliary Doublet Microtubule.
Authors: Meisheng Ma / Mihaela Stoyanova / Griffin Rademacher / Susan K Dutcher / Alan Brown / Rui Zhang /
Abstract: The axoneme of motile cilia is the largest macromolecular machine of eukaryotic cells. In humans, impaired axoneme function causes a range of ciliopathies. Axoneme assembly, structure, and motility ...The axoneme of motile cilia is the largest macromolecular machine of eukaryotic cells. In humans, impaired axoneme function causes a range of ciliopathies. Axoneme assembly, structure, and motility require a radially arranged set of doublet microtubules, each decorated in repeating patterns with non-tubulin components. We use single-particle cryo-electron microscopy to visualize and build an atomic model of the repeating structure of a native axonemal doublet microtubule, which reveals the identities, positions, repeat lengths, and interactions of 38 associated proteins, including 33 microtubule inner proteins (MIPs). The structure demonstrates how these proteins establish the unique architecture of doublet microtubules, maintain coherent periodicities along the axoneme, and stabilize the microtubules against the repeated mechanical stress induced by ciliary motility. Our work elucidates the architectural principles that underpin the assembly of this large, repetitive eukaryotic structure and provides a molecular basis for understanding the etiology of human ciliopathies.
History
DepositionAug 22, 2019-
Header (metadata) releaseSep 4, 2019-
Map releaseNov 13, 2019-
UpdateOct 23, 2024-
Current statusOct 23, 2024Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.02
  • Imaged by UCSF Chimera
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  • Surface view colored by cylindrical radius
  • Surface level: 0.02
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6u42
  • Surface level: 0.02
  • Imaged by UCSF Chimera
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  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-6u42
  • Imaged by Jmol
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_20631.png

Downloads & links

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Map

FileDownload / File: emd_20631.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationComposite cryo-EM density map of the 48-nm repeat doublet microtubule from Chlamydomonas reinhardtii
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.4 Å/pix.
x 512 pix.
= 718.336 Å		1.4 Å/pix.
x 512 pix.
= 718.336 Å		1.4 Å/pix.
x 512 pix.
= 718.336 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.403 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.02 / Movie #1: 0.02
Minimum - Maximum-0.042245477 - 0.1224482
Average (Standard dev.)0.0015316993 (±0.006318955)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions512512512
Spacing512512512
CellA=B=C: 718.336 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.4031.4031.403
M x/y/z512512512
origin x/y/z0.0000.0000.000
length x/y/z718.336718.336718.336
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ200200200
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS512512512
D min/max/mean-0.0420.1220.002

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Supplemental data

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Mask #1

Fileemd_20631_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Composite cryo-EM density map of the 48-nm repeat...

Fileemd_20631_half_map_1.map
AnnotationComposite cryo-EM density map of the 48-nm repeat doublet microtubule from Chlamydomonas reinhardtii (half map 1)
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Composite cryo-EM density map of the 48-nm repeat...

Fileemd_20631_half_map_2.map
AnnotationComposite cryo-EM density map of the 48-nm repeat doublet microtubule from Chlamydomonas reinhardtii (half map 2)
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Doublet microtubule from wild-type Chlamydomonas reinhardtii

EntireName: Doublet microtubule from wild-type Chlamydomonas reinhardtii
Components
  • Complex: Doublet microtubule from wild-type Chlamydomonas reinhardtii
    • Protein or peptide: Tubulin beta
    • Protein or peptide: Tubulin alpha
    • Protein or peptide: PACRG
    • Protein or peptide: FAP20
    • Protein or peptide: FAP53
    • Protein or peptide: FAP127
    • Protein or peptide: RIB43a
    • Protein or peptide: FAP112
    • Protein or peptide: FAP210
    • Protein or peptide: FAP45
    • Protein or peptide: RIB30
    • Protein or peptide: FAP363
    • Protein or peptide: FAP126
    • Protein or peptide: FAP52
    • Protein or peptide: FAP67
    • Protein or peptide: FAP85
    • Protein or peptide: FAP106
    • Protein or peptide: FAP115
    • Protein or peptide: FAP143
    • Protein or peptide: FAP161
    • Protein or peptide: FAP166
    • Protein or peptide: FAP252
    • Protein or peptide: FAP276
    • Protein or peptide: RIB72
    • Protein or peptide: FAP141
    • Protein or peptide: FAP222
    • Protein or peptide: FAP95
    • Protein or peptide: FAP182
    • Protein or peptide: FAP129
    • Protein or peptide: FAP21
    • Protein or peptide: FAP273
    • Protein or peptide: FAP107
    • Protein or peptide: RIB21
    • Protein or peptide: DC1
    • Protein or peptide: DC2
    • Protein or peptide: DC3
    • Protein or peptide: FAP68
    • Protein or peptide: FAP90
  • Ligand: GUANOSINE-5'-DIPHOSPHATE
  • Ligand: MAGNESIUM ION
  • Ligand: GUANOSINE-5'-TRIPHOSPHATE

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Supramolecule #1: Doublet microtubule from wild-type Chlamydomonas reinhardtii

SupramoleculeName: Doublet microtubule from wild-type Chlamydomonas reinhardtii
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#38
Source (natural)Organism: Chlamydomonas reinhardtii (plant) / Strain: CC-125

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Macromolecule #1: Tubulin beta

MacromoleculeName: Tubulin beta / type: protein_or_peptide / ID: 1 / Number of copies: 170 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 49.665809 KDa
SequenceString: MREIVHIQGG QCGNQIGAKF WEVVSDEHGI DPTGTYHGDS DLQLERINVY FNEATGGRYV PRAILMDLEP GTMDSVRSGP YGQIFRPDN FVFGQTGAGN NWAKGHYTEG AELIDSVLDV VRKEAESCDC LQGFQVCHSL GGGTGSGMGT LLISKIREEY P DRMMLTFS ...String:
MREIVHIQGG QCGNQIGAKF WEVVSDEHGI DPTGTYHGDS DLQLERINVY FNEATGGRYV PRAILMDLEP GTMDSVRSGP YGQIFRPDN FVFGQTGAGN NWAKGHYTEG AELIDSVLDV VRKEAESCDC LQGFQVCHSL GGGTGSGMGT LLISKIREEY P DRMMLTFS VVPSPKVSDT VVEPYNATLS VHQLVENADE CMVLDNEALY DICFRTLKLT TPTFGDLNHL ISAVMSGITC CL RFPGQLN ADLRKLAVNL IPFPRLHFFM VGFTPLTSRG SQQYRALTVP ELTQQMWDAK NMMCAADPRH GRYLTASALF RGR MSTKEV DEQMLNVQNK NSSYFVEWIP NNVKSSVCDI PPKGLKMSAT FIGNSTAIQE MFKRVSEQFT AMFRRKAFLH WYTG EGMDE MEFTEAESNM NDLVSEYQQY QDASAEEEGE FEGEEEEA

UniProtKB: Tubulin beta-1/beta-2 chain

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Macromolecule #2: Tubulin alpha

MacromoleculeName: Tubulin alpha / type: protein_or_peptide / ID: 2 / Number of copies: 170 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 49.638008 KDa
SequenceString: MREVISIHIG QAGIQVGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDAFN TFFSETGAGK HVPRCIFLDL EPTVVDEVRT GTYRQLFHP EQLISGKEDA ANNFARGHYT IGKEIVDLAL DRIRKLADNC TGLQGFLVFN AVGGGTGSGL GSLLLERLSV D YGKKSKLG ...String:
MREVISIHIG QAGIQVGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDAFN TFFSETGAGK HVPRCIFLDL EPTVVDEVRT GTYRQLFHP EQLISGKEDA ANNFARGHYT IGKEIVDLAL DRIRKLADNC TGLQGFLVFN AVGGGTGSGL GSLLLERLSV D YGKKSKLG FTVYPSPQVS TAVVEPYNSV LSTHSLLEHT DVAVMLDNEA IYDICRRSLD IERPTYTNLN RLIAQVISSL TA SLRFDGA LNVDITEFQT NLVPYPRIHF MLSSYAPIIS AEKAYHEQLS VAEITNAAFE PASMMVKCDP RHGKYMACCL MYR GDVVPK DVNASVATIK TKRTIQFVDW CPTGFKCGIN YQPPTVVPGG DLAKVQRAVC MISNSTAIGE IFSRLDHKFD LMYA KRAFV HWYVGEGMEE GEFSEAREDL AALEKDFEEV GAESAEGAGE GEGEEY

UniProtKB: Tubulin alpha-1 chain

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Macromolecule #3: PACRG

MacromoleculeName: PACRG / type: protein_or_peptide / ID: 3 / Number of copies: 8 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 34.215148 KDa
SequenceString: MNGDVAGSLF TSTYRNVKLA GKAPPAANLS GTGSCFDTTS LSPARAGAHK ALDVQKDELP VWSKSTLSYK YPAGRPNPTG FLKKGDGEM IKTKTGGFEE RKPSPPQAGA YKRRENPPNT AFRRFYERGD LPIAVDHRGS KNMIAWKVDI EKLDYHHYLP I FFDGIRET ...String:
MNGDVAGSLF TSTYRNVKLA GKAPPAANLS GTGSCFDTTS LSPARAGAHK ALDVQKDELP VWSKSTLSYK YPAGRPNPTG FLKKGDGEM IKTKTGGFEE RKPSPPQAGA YKRRENPPNT AFRRFYERGD LPIAVDHRGS KNMIAWKVDI EKLDYHHYLP I FFDGIRET QEPYRFLAVK GVEDMLRVGG SKILPVIPQL IIPIKTALNT RDHSVMCITL QLLQKLVLSA DLVGEALVPY YR QILPIFN LYKNKNKNLG DGIDYGQRNY DCLGELIADT LALFEQKGGD DAFINIKYMV PTYESSVNYA

UniProtKB: Parkin-co-regulated gene product

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Macromolecule #4: FAP20

MacromoleculeName: FAP20 / type: protein_or_peptide / ID: 4 / Number of copies: 7 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 22.193566 KDa
SequenceString:
MFKNAFQSGF LSVLYSIGSK PLEIWDKQVS NGHIKRITDA DIQSSVLEIM GQNVSTTYIT CPADPNKTLG IKLPFLVLII KNLNKYFSF EVQVLDDKNV RRRFRASNYQ STTRVKPFIC TMPMRLDSGW NQIQFNLSDF TRRAYGTNYI ETLRVQVHAN C RIRRIYFS DRLYSEEELP AEFKLFLPIQ KS

UniProtKB: Cilia- and flagella-associated protein 20

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Macromolecule #5: FAP53

MacromoleculeName: FAP53 / type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 56.655496 KDa
SequenceString: MNKSRAPPDA RIQKMRELEE RLANLKADRK VEQKMVAVAE FEARTTKKIV GNLVQQRYDA LKARAEADLN ARRQRLADKL DAEDLAMRQ ELLASKKTPE QRRAELAERA RALAATREAE RQALASTLYE KAFIQSCDVL RDENSKRILY RTIEERNAQI E HKMAQRIM ...String:
MNKSRAPPDA RIQKMRELEE RLANLKADRK VEQKMVAVAE FEARTTKKIV GNLVQQRYDA LKARAEADLN ARRQRLADKL DAEDLAMRQ ELLASKKTPE QRRAELAERA RALAATREAE RQALASTLYE KAFIQSCDVL RDENSKRILY RTIEERNAQI E HKMAQRIM EAEEKRMWHE MSEVERQKME QRYLDDKRRD REKREEVLRI LDEQVRQVNA RRAEASMLRR AEIAELNATW RQ MAADQEA ADVQERENMK KLAAELQEFN RIKQMEISEA ERSERELDLK ILQEALSKEA ADEAAELAFR ERRREEMRRY REQ LALMME KEREETAERD ALILKAQLEQ EAKRDAELAA RDEARRQLMA QVDAIRQIQI QEKLAKRLER AEEKAFERAQ MAEE VAKAE SDAAAKDAAD RKAGIQRRLE LQTMMVAKAH MKAAELDEKL AEGEATKRVE DQFKAKVNQT LSSTDPPVWH GRRKF DW

UniProtKB: Cilia- and flagella-associated protein 53

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Macromolecule #6: FAP127

MacromoleculeName: FAP127 / type: protein_or_peptide / ID: 6 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 60.027434 KDa
SequenceString: MSACDCTCHD GGTRAPKRGH LARAVEQREA ARRIAEAAKS EINRYFNDQK VYDTVAANAV KDDFKRKGRE FKERASEAQM LESLYQSER QKTLNAIRAE EEERIAVAMA RKQQEKDRSE REVQRLREQS DELRSLAEKI RVARVNKERS DQLVEKKIIG E QQQEYDRA ...String:
MSACDCTCHD GGTRAPKRGH LARAVEQREA ARRIAEAAKS EINRYFNDQK VYDTVAANAV KDDFKRKGRE FKERASEAQM LESLYQSER QKTLNAIRAE EEERIAVAMA RKQQEKDRSE REVQRLREQS DELRSLAEKI RVARVNKERS DQLVEKKIIG E QQQEYDRA FNQFVAGAAA EAEAQEQENQ AKRREANVRA RMVLEEQMQE KAEAARLAEL EAVRERAMID EVVRRIMEED AA EMATKRQ RQEETKDFIS HFLEQQDELR RKEREAAAAE DKKIQEYWQS VREREREEAE RKAMRKEIAD RMYEKVKREM EAE MARREE EEELINMLRQ EELEAKRRQE DEDRKRKAEE SKEEMRRANE YQMKLKEERE AAFRAEEEAF RQRMLAKFAE DEKL EQMNA QKRRMRMAEH AREVQRLIDE KRQAFEAAKA REEAEDAAKR SEDDRVRGLV EEERKKLLRE AAELKDFLPR GVLRD QADV DFISNVLEEM ALNRAKATQG R

UniProtKB: Meiosis-specific nuclear structural protein 1

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Macromolecule #7: RIB43a

MacromoleculeName: RIB43a / type: protein_or_peptide / ID: 7 / Number of copies: 5 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 42.712711 KDa
SequenceString: MGRLVAQVPN EDPERLKRVL DAKWRTIGVD KETLELQAQE KKDREQAEKD RDEAFARLTA YFDDQLTLMQ QEADQIRKAY NHDTEAFRQ QQQLKHTRRE WDINRPDAKQ LDMPGRVGDD DNRLGPSSLQ KFDGEDLTAG DRKKAQIEQS VNWWAEQTAI R DALRAAEK ...String:
MGRLVAQVPN EDPERLKRVL DAKWRTIGVD KETLELQAQE KKDREQAEKD RDEAFARLTA YFDDQLTLMQ QEADQIRKAY NHDTEAFRQ QQQLKHTRRE WDINRPDAKQ LDMPGRVGDD DNRLGPSSLQ KFDGEDLTAG DRKKAQIEQS VNWWAEQTAI R DALRAAEK EAETAHAELV KYQDLLQQTA KSEEAAVRRE VARATADYNK RLAEEKRLRE YAAKQADLAA NMAEMEATIT SS FMTEDPN MAASSMSAYR VRKDHYKGMT ETEKQAILDA QLAQMEEKKA RRAQEQLENM MYARTQHDIQ RALQEQAQRV DDF KKAQMA RASEILKKQQ EEKAERDKHL ASLYRNKMAP EFFTQFGTSH R

UniProtKB: Flagellar protofilament ribbon protein

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Macromolecule #8: FAP112

MacromoleculeName: FAP112 / type: protein_or_peptide / ID: 8 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 53.126352 KDa
SequenceString: MPSPAREKLM TIKAMEEAKG RSQHARAPAI FRDTALDTHK SIQPEYFGPS TVPEKKEFST RLSSGRTRSV TKHQRAAMEA LQRTSQMAG QGEVRTVFMP TAEQMPVCAA AGERRGNVAN SEWALLDTLE VNLYLNEKDA RLRSQKAVQQ TQRAILDTQV G MLAQAKLA ...String:
MPSPAREKLM TIKAMEEAKG RSQHARAPAI FRDTALDTHK SIQPEYFGPS TVPEKKEFST RLSSGRTRSV TKHQRAAMEA LQRTSQMAG QGEVRTVFMP TAEQMPVCAA AGERRGNVAN SEWALLDTLE VNLYLNEKDA RLRSQKAVQQ TQRAILDTQV G MLAQAKLA AETAKAAERV ELLATVAAHQ AEERQRAEEQ RAALTRLRTD REAMLAETRV QREAALSRKR EEEAKLVAAA QA QLEADRQ AAARKAAELK EQAAKTMADN EARLVARKAA EAAQRVADAE TTKRMIEMAE AQDRARDRNM KSFHDMIQAR ARG VGQKAV DDRRDRLERE ERLIAEAERA AAQREAERAA AEAERKARLK SDLVSGNEAL KRAKAEKLAV EREAEARERA AAEQ RVLAE KEAAERQMAG MRERATATKR FVAGQAAAVA ERAKTDDIFM SEQERLLNKR LLEQAVATVQ RPMQYSVKLY

UniProtKB: Uncharacterized protein

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Macromolecule #9: FAP210

MacromoleculeName: FAP210 / type: protein_or_peptide / ID: 9 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 53.637711 KDa
SequenceString: MATYSVISKA QLDRIRDKVA PVGQDTFSEE RQRLKQLSDD RSSQWPNTLS AQRARKEKTR QERQAAEEAE RVELDRQEAE IRAEQRRIQ IERANKILFD ETDRVKGFHS KMLLSDVMHE NEQLKEIKRQ IEVLKRAQEQ AFVEQQRQAL EAAEAAEVRK L EDTRRRAM ...String:
MATYSVISKA QLDRIRDKVA PVGQDTFSEE RQRLKQLSDD RSSQWPNTLS AQRARKEKTR QERQAAEEAE RVELDRQEAE IRAEQRRIQ IERANKILFD ETDRVKGFHS KMLLSDVMHE NEQLKEIKRQ IEVLKRAQEQ AFVEQQRQAL EAAEAAEVRK L EDTRRRAM AQREVQLQQL EELKAKILGE RAADRTEGET LRRKALEEAD ELRRKEEARL AKQRQLADDT KAANAALQAF RL KEVERSK EQEAAMEAYA RKKQELADER ARREAEKRAA KDAERKRVAD MMESNYMAWH TKEEARLARD VAAAEQKAAA DEE ARRKRA ADLAVAIDQS RQAQLRAKAQ AKAAEKAEEA EYLSAWSTRT KQLKAEEDEE KLKRIAVGKQ LAAFQLRQAA IKAR KMADA RIAELQEAAQ LALSVAEEED IFLRYAHECI EEYRRQGKPT VPMELHLRKK TNLETMR

UniProtKB: Trichohyalin-plectin-homology domain-containing protein

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Macromolecule #10: FAP45

MacromoleculeName: FAP45 / type: protein_or_peptide / ID: 10 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 59.041469 KDa
SequenceString: MPQTPPRSGG YRSGKQSYVD ESLFGGSKRT GAAQVETLDS LKLTAPTRTI SPKDRDVVTL TKGDLTRMLK ASPIMTAEDV AAAKREAEA KREQLQAVSK ARKEKMLKLE EEAKKQAPPT ETEILQRQLN DATRSRATHM MLEQKDPVKH MNQMMLYSKC V TIRDAQIE ...String:
MPQTPPRSGG YRSGKQSYVD ESLFGGSKRT GAAQVETLDS LKLTAPTRTI SPKDRDVVTL TKGDLTRMLK ASPIMTAEDV AAAKREAEA KREQLQAVSK ARKEKMLKLE EEAKKQAPPT ETEILQRQLN DATRSRATHM MLEQKDPVKH MNQMMLYSKC V TIRDAQIE EKKQMLAEEE EEQRRLDLMM EIERVKALEQ YEARERQRVE ERRKGAAVLS EQIKERERER IRQEELRDQE RL QMLREIE RLKEEEMQAQ IEKKIQAKQL MEEVAAANSE QIKRKEGMKV REKEEDLRIA DYILQKEMRE QSLAAEKERI AKE KEMETA RLRAMQERAA DKQSELDELR ARRYQEAKER EWRQKERAYA ERQASMQQEL ANARTAQQAS KLKQKAEMAR LEHD EFMRV LDVNRAKEYD ELQQTVNAMT LNSKYKEELL AQIQANEERR KRERSHYLEE GARLREAAEK ERQLLLQIKD RKLGE LESA GVPGKYRAEL EKMKIRS

UniProtKB: Cilia- and flagella-associated protein 45

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Macromolecule #11: RIB30

MacromoleculeName: RIB30 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 29.859654 KDa
SequenceString: MLNVTGGRRP VASWRTPPGF LERLADAWPA VLDGAVEQAG GDPARVTRDS FLAALREALP GLSAAEDDYA RQVSLSVIQQ VRGSNVFFP DLDYLQAALL QGRVPPQELD QPRSTLSLAT FTTTTRSGTK SLDLFKTTGV TWKIPKGFLN RYNDCNHEVL R RAAALVGA ...String:
MLNVTGGRRP VASWRTPPGF LERLADAWPA VLDGAVEQAG GDPARVTRDS FLAALREALP GLSAAEDDYA RQVSLSVIQQ VRGSNVFFP DLDYLQAALL QGRVPPQELD QPRSTLSLAT FTTTTRSGTK SLDLFKTTGV TWKIPKGFLN RYNDCNHEVL R RAAALVGA RHDGARDVVA GVWGRVDVPT FVEACRQVLG EISADEEEYL IALASEQVQD GTAYIRDLPF LDKCIQNGKT PT SIKGPEL LPSIFLNDTT SGKTDGMTLR HTGGRIF

UniProtKB: Uncharacterized protein

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Macromolecule #12: FAP363

MacromoleculeName: FAP363 / type: protein_or_peptide / ID: 12 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 43.879367 KDa
SequenceString: MLPTDTMKGT FAPGTTKRKT VNMYDTQSST FQPRPEGPFE AEHITDQPAA HEHFDTTREI KLKDPKGMDL PATSYVEHYP PKTALLPGE PLELALGGVP FTATSTYDNE FWNKPRAPRP VEPLTYTHRP GPMITRDTTN QDTYKPFEMA PQPGSMTGRP T RNATAPPP ...String:
MLPTDTMKGT FAPGTTKRKT VNMYDTQSST FQPRPEGPFE AEHITDQPAA HEHFDTTREI KLKDPKGMDL PATSYVEHYP PKTALLPGE PLELALGGVP FTATSTYDNE FWNKPRAPRP VEPLTYTHRP GPMITRDTTN QDTYKPFEMA PQPGSMTGRP T RNATAPPP AMPSIYDTTY RAHYIPKEGE PRVGPGTIPP KDPLPWLNDG TTYRNDYAPK GLALLAPADY DPYNPFPFGG TT EYRAEYP AKEADPQLPP LTGVRSREGL ELPLPRRSLG VEFVHKGVSD RYFVLIPRTL DSPCSARQVF TTVHDNQEQA CIL ILYGDD PVASNNTLLG QFDIVNIPPA PKDVPRIEVT FHLSRDMFLT VEARDLDTAR HKRWLQRGDI VVLRH

UniProtKB: Flagellar associated protein

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Macromolecule #13: FAP126

MacromoleculeName: FAP126 / type: protein_or_peptide / ID: 13 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 15.435397 KDa
SequenceString:
MSRSYPGEQV EHAFNSKRLK NWEVPAVDKS QAISTSTGTR FGTLQPRSGR TQFIVDDNGH LKSGVPKLEK SAFNFTQTTP VFMDSAPRW PKENPTWPKN MKATMGYKGI QSNYLPTNTV TLKAVEVPGT TERNFNFM

UniProtKB: Protein Flattop homolog

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Macromolecule #14: FAP52

MacromoleculeName: FAP52 / type: protein_or_peptide / ID: 14 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 68.546508 KDa
SequenceString: MAEPLVLNSV IGFGGAIENG LIAHPDGRTI IYPLGSTIVL RDRADPRSQE FLQGHSDKVS CLALSRSGRY LASGQITYMG FTADIIIWD LESKQLIHRM ALHKVKVQAL DFSCDERFLA SLGGQDDNAL VLWDVASGNA ICGSPCNTNF TNCVKFFNNS P DKLITAGN ...String:
MAEPLVLNSV IGFGGAIENG LIAHPDGRTI IYPLGSTIVL RDRADPRSQE FLQGHSDKVS CLALSRSGRY LASGQITYMG FTADIIIWD LESKQLIHRM ALHKVKVQAL DFSCDERFLA SLGGQDDNAL VLWDVASGNA ICGSPCNTNF TNCVKFFNNS P DKLITAGN FNMNVWTYDA GNNKLRPTDA TLGTLKRVFK SVVVDANDEY AYCGTTTGDV LQIALERVLF KNTGPAKGNV QM GVTATCE VPTGDILVGG GDGSLQVLRT VPEPSSTNPK LLRKMPALAG TKVEGAITSI ALADMNARGF TFFVGTAMCN IYK VTYEPA TSRLKEELVQ TAHNDKINGM AFPNEYSEVF ATCGTGFIRL WHLTTCRELL RIAVPNLECF CIAFTTDGSA ILSG WSDGK IRAFGPQSGK IIFTINDAHQ KAVTAIASTA DSSRILSGGE EGMVRVWRIG RTSQTLEASM KDHKGPVNCI RIKGS GDEC VSASSDGSCI LWDLHTFKRR TSLFANTFFK SVVYHPDESQ LVTAGTDRKV TYWDAYDGNA IRIIDGSDLD EVNALA VDR DGEALVSGGG DKLVKLWGYD EGHCYFVGVA HSGAITAVGV TPDKQRIVSV GTEGGIFIWD YQRPQTLADI

UniProtKB: Cilia- and flagella-associated protein 52

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Macromolecule #15: FAP67

MacromoleculeName: FAP67 / type: protein_or_peptide / ID: 15 / Number of copies: 2 / Enantiomer: LEVO / EC number: nucleoside-diphosphate kinase
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 41.994797 KDa
SequenceString: MAANLRYCFI SEWLDPASGI LWKYQLFYYP ESKEVEMVDI KNRRHFLKRT KYEELKPSLL FLGSVVTVFS RQLKLTEYGD EFTRNRMES QSERTLAMIK PDAYKNMGKI INAICQSGFL ISKLRIGKLS KEEAGEFYAV HAGKPFVDRL TDFMSSGRVV A MELVAPGA ...String:
MAANLRYCFI SEWLDPASGI LWKYQLFYYP ESKEVEMVDI KNRRHFLKRT KYEELKPSLL FLGSVVTVFS RQLKLTEYGD EFTRNRMES QSERTLAMIK PDAYKNMGKI INAICQSGFL ISKLRIGKLS KEEAGEFYAV HAGKPFVDRL TDFMSSGRVV A MELVAPGA IRKWRELIGP TDSNQARAEA PGSLRAQFGT DKTFNACHGS DAPDTAAEEC NFWFGPGRYP GKCDLAAGTT LC LVKPHLV ADGAAGLVID LIQESFEVTA GGLYNLDRNA AAEFLEVYKG VLPAGDFNSM VEQLTSGACI ALEVADRDGA DAV EPFRQL AGPLDPELGR VLRPASLRAR FGLDAVRNGV HCTDLPEDGV LEVNYFFTIL PTAS

UniProtKB: Nucleoside diphosphate kinase

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Macromolecule #16: FAP85

MacromoleculeName: FAP85 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 22.228705 KDa
SequenceString: MSFFGLTSFG PQDPVKDRVK TSHEYVFHTF PIEHYTDTFT KYTIGDSDIS VALEVDGATH IVRAKLGDIL KDILGRQPRK HELDAWFTH LDFDRSGVMG IDEYIKGVER LLEFSATGVT PATYSSFDTQ RTDWVRHTRV GYEAQQTLRG PMTTAQEVGW H TAKPAPPE ...String:
MSFFGLTSFG PQDPVKDRVK TSHEYVFHTF PIEHYTDTFT KYTIGDSDIS VALEVDGATH IVRAKLGDIL KDILGRQPRK HELDAWFTH LDFDRSGVMG IDEYIKGVER LLEFSATGVT PATYSSFDTQ RTDWVRHTRV GYEAQQTLRG PMTTAQEVGW H TAKPAPPE TAQRRTLGST DVTQREGHTA ASYYGHFLG

UniProtKB: Uncharacterized protein

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Macromolecule #17: FAP106

MacromoleculeName: FAP106 / type: protein_or_peptide / ID: 17 / Number of copies: 5 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 27.019803 KDa
SequenceString: MQEESVYALI PQPQEVPQRP AMHTSKFGGK THPAQFDFGQ NKVQPHATMG RPDGANGPAF LHAHEKEPKL PSPGPPSNPK QKIRPPVPA KEEKPTMGLT SNKNFITANA VDVILAKPGK VPQPEFQWTQ KPDYGKVPMY LKRNKDRVAK EKEHFTQYLR M REAPEANA ...String:
MQEESVYALI PQPQEVPQRP AMHTSKFGGK THPAQFDFGQ NKVQPHATMG RPDGANGPAF LHAHEKEPKL PSPGPPSNPK QKIRPPVPA KEEKPTMGLT SNKNFITANA VDVILAKPGK VPQPEFQWTQ KPDYGKVPMY LKRNKDRVAK EKEHFTQYLR M REAPEANA HVSQLSPEDR QQLVRHLKAK WGSVNTAYQG LSLSVDSAVK KGRKEAMERE LAEIERDIRT LERGEVVLVV DD

UniProtKB: Uncharacterized protein

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Macromolecule #18: FAP115

MacromoleculeName: FAP115 / type: protein_or_peptide / ID: 18 / Number of copies: 7 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 26.647648 KDa
SequenceString: MVADVTLESA MKRLKQHVYK QRVRVKDFIM DFDKLNSGYV FPNHFLSALS MAGIDKYLSP KELEVICETY KVQRDATLVM VDHRTFLHE LELVFTVPHL EKDPLVEVAT EPADLLDKTR YLKSSRVLQP PEREEAVQAV ICRLSDVCYK RGQPVKAFFD D AARDDHSA ...String:
MVADVTLESA MKRLKQHVYK QRVRVKDFIM DFDKLNSGYV FPNHFLSALS MAGIDKYLSP KELEVICETY KVQRDATLVM VDHRTFLHE LELVFTVPHL EKDPLVEVAT EPADLLDKTR YLKSSRVLQP PEREEAVQAV ICRLSDVCYK RGQPVKAFFD D AARDDHSA KLFGHVTLPQ FRQVLTTKLD WVVSDPEVSL LAAKFLHEDK PEFINYIAFS CTVDPPERYY EQ

UniProtKB: Uncharacterized protein

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Macromolecule #19: FAP143

MacromoleculeName: FAP143 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 29.21652 KDa
SequenceString: MAEETQPYTS YNKQDEVPTL IGNWVEEREL KELTGVTRNL AASQALKDTS DGTSPTRSLG DALTATHPRV IEHVQAQTHA ADWQSTVQA TYRPPSDATR NAAAYVNTSK MGPRERMLHE QLMREAQDLP PELQATLTGP AVPVTTASTY GADFHQHDLT G IVVGAKVM ...String:
MAEETQPYTS YNKQDEVPTL IGNWVEEREL KELTGVTRNL AASQALKDTS DGTSPTRSLG DALTATHPRV IEHVQAQTHA ADWQSTVQA TYRPPSDATR NAAAYVNTSK MGPRERMLHE QLMREAQDLP PELQATLTGP AVPVTTASTY GADFHQHDLT G IVVGAKVM KDRDGRPAVR DPTFLAETQM MKKDAADRLM GETARQSGAR DTTMLPNPDV PVTIYTEAVA NKTYGGVFPG TT TLNTAAP FGKSTNFSKP MSDYSKVVVD E

UniProtKB: Flagellar associated protein

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Macromolecule #20: FAP161

MacromoleculeName: FAP161 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 42.849578 KDa
SequenceString: MRDTTKYNAS CLIGNWAEDR ELQRAILKDM LSKKGTGTLK LDAFRTRMAA ALSDLELTKV ADDPYIHFGD VVQLVHVDTG CVLAGDPAD ADTRTGESTC AATAAPDVRA PCPRNSLILL PYVPPKTATA LEPPYDDAIV HYGQKVRLAL HPGAAGDPVD S GGGPRPVC ...String:
MRDTTKYNAS CLIGNWAEDR ELQRAILKDM LSKKGTGTLK LDAFRTRMAA ALSDLELTKV ADDPYIHFGD VVQLVHVDTG CVLAGDPAD ADTRTGESTC AATAAPDVRA PCPRNSLILL PYVPPKTATA LEPPYDDAIV HYGQKVRLAL HPGAAGDPVD S GGGPRPVC LFSKPVSTTH AARYSRQQLV GFTARTDSFD CVWTVVTPDP AQRAAAEGVE VAAGAPVLLV HCATQKPLCL EA ARYPNDY GVELEVSARS ALGPGLKLAM EQMAMGVQKG FLPKGEQTDN YWTFVAGSKV EALPPPSAGG DEAYSFLDGL VLE LASRPG SLSLLERKLV TLENNQSLMS AEDFKLVLRQ VGSQLPEDGI AALLTRYAPA GSRPGTRLDA AAFRNDLRAA STAA GAR

UniProtKB: Uncharacterized protein

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Macromolecule #21: FAP166

MacromoleculeName: FAP166 / type: protein_or_peptide / ID: 21 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 23.303205 KDa
SequenceString: MSLTLNGLDE SMRRMQGYEV TRAPEDVGNS IPNFKEGIFT YKGSRQAPWK SEQTHSFSLP NAYTARVLNG TIVHTGGATE MAITTHHTV ERPMMPPGTI RGSTWVKPQY IPTDDPALDE LHAVAYVVSP QLPALMDACN SYHLHSADGW ITTAGFMTAA R RAGLTLSR ...String:
MSLTLNGLDE SMRRMQGYEV TRAPEDVGNS IPNFKEGIFT YKGSRQAPWK SEQTHSFSLP NAYTARVLNG TIVHTGGATE MAITTHHTV ERPMMPPGTI RGSTWVKPQY IPTDDPALDE LHAVAYVVSP QLPALMDACN SYHLHSADGW ITTAGFMTAA R RAGLTLSR AEYLALERAL TKDTMGRINY LQLEALVQAV TAADQTGEGG AEPAAE

UniProtKB: Flagellar associated protein

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Macromolecule #22: FAP252

MacromoleculeName: FAP252 / type: protein_or_peptide / ID: 22 / Number of copies: 8 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 39.507379 KDa
SequenceString: MSGSTTKVPF TEPEWKIPGY TGYVQGLQET YKKTPVMAQL ETKDPSPESF IYTRTQTAPK PSPVRDPCNN PENFKKPQPG NLWPALQET AIQASFKPPT SNIALGDERI IPFRTSYGVD FKAPFNGTEQ LRSPNRNEDL VKTTSSLTNI YKSSFNRVGE K RLQKMIST ...String:
MSGSTTKVPF TEPEWKIPGY TGYVQGLQET YKKTPVMAQL ETKDPSPESF IYTRTQTAPK PSPVRDPCNN PENFKKPQPG NLWPALQET AIQASFKPPT SNIALGDERI IPFRTSYGVD FKAPFNGTEQ LRSPNRNEDL VKTTSSLTNI YKSSFNRVGE K RLQKMIST MRERMEAKLG NSNNNAFRMR KLFKMYDNDG SGRVHFEDFR NMAETFGMQL DDDSLMALYF VYDPEGSGYL EY EALVAQL MSPSDFAFYK GYVDYSQDKA DEARRVELLS QLKKKIGPVA GDLERLLKAF DSAGNGQVSR HDLVAGCASV GVV LGDKDF ETLAPVMTYN SDGLIDYAAF CAVFNS

UniProtKB: EF-hand domain-containing protein

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Macromolecule #23: FAP276

MacromoleculeName: FAP276 / type: protein_or_peptide / ID: 23 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 10.073385 KDa
SequenceString:
MDLKQQVKNY TMTIRNTRPP TMIKEQDKSE FSHFRALQVL ANGDEVPYEA TLRNVIHDGA RQPKLPPRQT QKHPGYIRNE SGGFFTS

UniProtKB: Flagellar associated protein

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Macromolecule #24: RIB72

MacromoleculeName: RIB72 / type: protein_or_peptide / ID: 24 / Number of copies: 8 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 72.082609 KDa
SequenceString: MSGGAPPIPK LPGYTVCLPQ SLSDKGFKKG QTLTYVNGYQ REDALAQVKD LPERIAQATD GALASGAMGT GVLPASMMQD TATKLPQWV ENDRKVLRFY GYFKESVVES NMENHRIRKV ILYYYLEDDS MHVAEPRQDN SGIPQGVFIK RHRVTRDDGS F FNPGDFSV ...String:
MSGGAPPIPK LPGYTVCLPQ SLSDKGFKKG QTLTYVNGYQ REDALAQVKD LPERIAQATD GALASGAMGT GVLPASMMQD TATKLPQWV ENDRKVLRFY GYFKESVVES NMENHRIRKV ILYYYLEDDS MHVAEPRQDN SGIPQGVFIK RHRVTRDDGS F FNPGDFSV GDTVSIYGRN FYLVDADSFT REFMAARGKE QGGPLPYPGD PVDVYRATFG MNRGRAPQAR EDSPGSTTRR VN DFKAYVE ARLGKPSHLL DGDRLRQFLE NNKKVLRFWC VWDERTTMYG DRRPYVLHYY LEDDSVEVLE INENNSGRDP FPV FLKRGP LPKVAVKTNT TLNPKFRKDQ CYNAGDFRLG LFINVLGRDF YLHDADTFTK QWYKDNLGYT DEEMSPVDVK EPIL PKPRA AVPPFNGYGT IEDSLQNCLS LVPKPPKRDL HKLMNKDKII LRFVVKMVDT DTHKHSATDL ARRFILSYFM MDDSN LIFE PPVRNTGIAG GKFLERQKIY KPRSEEIYTY LDLYVGATIE VFNRTFELLE ADEYTLTYME NYKDIFVMAD TDVLIR SLK AQVSGKEDAV RSSVIAADKS GSGALTGDDL EAGLQSAGLK FTRHQAISLK RRLDKNKTGT ISIEEFLGLL GL

UniProtKB: RIB72 protein

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Macromolecule #25: FAP141

MacromoleculeName: FAP141 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 12.740544 KDa
SequenceString:
MSYMPRNVRE TVERNEMYAR LQKQNKEELR TAIIAQWTER DLQRPPPSTG LTKASITLAG TSSDRDAGIK SGVQTVKAAR QARLRELFE REALMYEKEL NARGLSLVKP RD

UniProtKB: Flagellar associated protein

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Macromolecule #26: FAP222

MacromoleculeName: FAP222 / type: protein_or_peptide / ID: 26 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 15.920962 KDa
SequenceString:
MATNSTGPWA TGTFSPNSTG TVTQYNHPMF VSQRLTGNFT SQFEMNSLPS HKYETLPIRS GHLPGYQGHV PGGVGAIAQR KPAAAMHTM THLATSGSLP KGSPQTDMSL VDLRPEQRSM AKVYMYAEGA KTSFLKFPTP KTFDHRN

UniProtKB: Uncharacterized protein

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Macromolecule #27: FAP95

MacromoleculeName: FAP95 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 25.576527 KDa
SequenceString: MAAYAHNDGA PDISQAFQNT VLVKNWYEDR FQSQVASATG RTLRELPTHE RVVHKAVPPG HPGLFQTTKQ AAEEKLLTTP PPAKVKKPS MYTEANVAER LQTYGLADNI HYTIGPNAAT EASWAPVHNL TTTNKEFYEI KPEAARAADP DTFRASGPSP F AKTGFCAK ...String:
MAAYAHNDGA PDISQAFQNT VLVKNWYEDR FQSQVASATG RTLRELPTHE RVVHKAVPPG HPGLFQTTKQ AAEEKLLTTP PPAKVKKPS MYTEANVAER LQTYGLADNI HYTIGPNAAT EASWAPVHNL TTTNKEFYEI KPEAARAADP DTFRASGPSP F AKTGFCAK SVKGEASDET TVAGGKGARG EITRRPGESG NPYGVSVFVD EYGKWGSAIQ GMPLTETRAR MQTKYFP

UniProtKB: Uncharacterized protein

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Macromolecule #28: FAP182

MacromoleculeName: FAP182 / type: protein_or_peptide / ID: 28 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 48.843969 KDa
SequenceString: MAQVAAVIVA TSTGRTLADE LSSRGSYTHL IEGPDGVPKV LLGVNGQPVL NHWLAAIKAV PRLTPIEEKV FILCNENNVE HVRAWAADP RTSLGGFPLD NVLTNGSDDS LGFAGDLAAF LAAAPPAAQL SSASLVVVEG DGLVGPGFGL SRVVEHTVVR G KDTLTYMA ...String:
MAQVAAVIVA TSTGRTLADE LSSRGSYTHL IEGPDGVPKV LLGVNGQPVL NHWLAAIKAV PRLTPIEEKV FILCNENNVE HVRAWAADP RTSLGGFPLD NVLTNGSDDS LGFAGDLAAF LAAAPPAAQL SSASLVVVEG DGLVGPGFGL SRVVEHTVVR G KDTLTYMA APEGMPLEGQ AVLGLEDAAN AYQTASQRVE GLDAAANGIA DPMAFTPVLA PVAVLRPETV ARAAGSAGAG PS PYGTCGL GYMLAGLRPG DVAHPPMYAM PVDSCFRLGD AYSLQLASNF FAYYATEKAG GKGEAAKALD AARRLAQLNE ART MAGGSL AGAVKLVREV ESARPPEPCV DAAQRKLYNA FFQSWLAGDR HHDVGATVGR GGVSAAGGGD GAGAGLPLRF ADVT TRKHN PKQQHPVYQT SNSIYGAKAP SQLDMPLSYS SSSQAFTRAF PVTAAKNSCM VTSVTRSNVH KALDDF

UniProtKB: Uncharacterized protein

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Macromolecule #29: FAP129

MacromoleculeName: FAP129 / type: protein_or_peptide / ID: 29 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 51.330992 KDa
SequenceString: MVHKGPNQAG NKGLLTYNNA VGIPGYTGFM PSTNALALPV KGFEHTGRPA ASAEVEKLTV KSVDPRKTSQ YADDYHKKPA DTKAFSKTG GGYWISQRVL PPHTAFTATT TYRAETLNAE PNTAAILDRS QGLASTLVGY EAARQAGEVR RSISADPRAR A EDTARGIG ...String:
MVHKGPNQAG NKGLLTYNNA VGIPGYTGFM PSTNALALPV KGFEHTGRPA ASAEVEKLTV KSVDPRKTSQ YADDYHKKPA DTKAFSKTG GGYWISQRVL PPHTAFTATT TYRAETLNAE PNTAAILDRS QGLASTLVGY EAARQAGEVR RSISADPRAR A EDTARGIG TQTVLGRPGS GGNTSILATV AAAAPSSPQA GSSVMVSSAR RPATVPTKYG ELPGYQTTYG AATDKMARMQ AD NELNGTG SFAPSNMGDP RFKTLPRVMN PGMGRNYSSY VAEYGGDGHD PMARQAANKD TMTRISVTRD LAGGTTRNVS HIP RYTGHI PASEYATPEA RAQGEAAEPR PDHKSQALTY TLDQYPRGRL PGYTGFKAQA PANIDAGLKH SMKLPCHSTT SGDA TLRGT QFGVPHQDHT HYINSRAGLN SFFSNSVVGT EFVSDNGLFN AQVYYKEAKS QGALGIKTAQ PSKLTHYGAP FRAAA SMV

UniProtKB: Flagellar associated protein

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Macromolecule #30: FAP21

MacromoleculeName: FAP21 / type: protein_or_peptide / ID: 30 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 49.657867 KDa
SequenceString: MSLTTQSLRR TNYEAEMTQP QIPPAGITGK LHETAKDALT WNDERPSTPD DIKKYRQSTV HEPGKIVRHP GHADDPVPQG PFGVKSAAS GGQNINEALK NYPDSELARW KLEQAEGVYA SAQREPLGAG YVRGHRLPEG LGSERPFGVT YDARGKDLSR Q AAAVIFPT ...String:
MSLTTQSLRR TNYEAEMTQP QIPPAGITGK LHETAKDALT WNDERPSTPD DIKKYRQSTV HEPGKIVRHP GHADDPVPQG PFGVKSAAS GGQNINEALK NYPDSELARW KLEQAEGVYA SAQREPLGAG YVRGHRLPEG LGSERPFGVT YDARGKDLSR Q AAAVIFPT DRPAEEDAAT RAMYTRSHQD FQPGEQRRRD YNWDAAGIDP AQHRFGAVDR NGVGDGVRKA LQPGLDPSLQ AP KVLPKLH EDFKATATDY LGRPRQLGTG DRPQLAPDHA FGQPSMRKGR EPGVGELLTG RFGADEQQPD ADLGKSLREG YRN QPKPGD EGRAFGVPTI RTDVRLPRLR SVANACNYGN EPDAGQVLRP PRAADLGISD EAFVALRPKS ELRQLVDEAG LALS DADFE AAWALAAEAD GGAAAAGEGG GAAEGPEGRA CVDTFFRARH HLLAQTLQIE PTF

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Macromolecule #31: FAP273

MacromoleculeName: FAP273 / type: protein_or_peptide / ID: 31 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 15.469539 KDa
SequenceString:
MSILGPADRR PELALTGTTI SHLKTWRTEY LDEYSDIKLA AGVPEQRMEM AGITAHIGTI TGRHTHMHKE TTRLPTGHPP SSTYRAQDA VPIGTMTRGT GTITKLGDSC LYDKEQTWAH WRVAVDGKPA DTRRKYRGVS

UniProtKB: Uncharacterized protein

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Macromolecule #32: FAP107

MacromoleculeName: FAP107 / type: protein_or_peptide / ID: 32 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 25.937781 KDa
SequenceString: MQGDRWSRNC GSGGVGHSGT VNEYRSGVLI GNFVENAAKT TGRMGETILS HTGPGAQTGI PTTTQKRSYT AEGKTGEYLV EASTRHDLN QPGVKGELLT RHGRFDEPPV QCLGTTYQLT YGRADGTDRR VQSYLWHGRK QVDYFVPHST GGPSTLSLTA R KQQEWGTQ ...String:
MQGDRWSRNC GSGGVGHSGT VNEYRSGVLI GNFVENAAKT TGRMGETILS HTGPGAQTGI PTTTQKRSYT AEGKTGEYLV EASTRHDLN QPGVKGELLT RHGRFDEPPV QCLGTTYQLT YGRADGTDRR VQSYLWHGRK QVDYFVPHST GGPSTLSLTA R KQQEWGTQ GATDAYLTTK MAATQPAALA TAENPTRTQT LRPLGDSGLM PQPGQKPKGF ARDELDKPHH RTGLRVNYRS

UniProtKB: Uncharacterized protein

+
Macromolecule #33: RIB21

MacromoleculeName: RIB21 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 20.949049 KDa
SequenceString:
MDATTKTLKS TTRVDNSTNP NFKHTSTFHT RGQWTPESPP PLTSTYTIFH GERPELPRYV PKYAVSPETA ALTSRHGSSP YSFRATAER AGSTPDGRAT YRFSGLPAGV SPYSTGTKLS SSTLGSSGLP PVQYKSYLTE YVDEYREPLE QLDTQRSLTL K YGTTGGYR TTQRSTRSDG QPKYQTRVVA F

UniProtKB: Flagellar associated protein

+
Macromolecule #34: DC1

MacromoleculeName: DC1 / type: protein_or_peptide / ID: 34 / Number of copies: 2 / Enantiomer: LEVO
EC number: Transferases; Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 83.51818 KDa
SequenceString: MAQKSTLKLP RLRTKEELLK TSPELCKLLG EDSDDGRSMS PFTAPPPAGT VKPPSRGLPA VSTKATKGPG MDTPRGLGEE ELTEEELLR LELEKIKNER QVLLDSIKLV KAQAGTAGGE AQQNDIKALR RELELKKAKL NELHEDVRRK ENVLNKQRDD T TDASRLTP ...String:
MAQKSTLKLP RLRTKEELLK TSPELCKLLG EDSDDGRSMS PFTAPPPAGT VKPPSRGLPA VSTKATKGPG MDTPRGLGEE ELTEEELLR LELEKIKNER QVLLDSIKLV KAQAGTAGGE AQQNDIKALR RELELKKAKL NELHEDVRRK ENVLNKQRDD T TDASRLTP GELSEEQAYI QQLQDEMKQI DEELVEAEAK NRLYYLLGER TRREHLAMDM KVRASQQLKK DSADDLYTLT AH FNEMRAA KEQAERELAR MKRMLEETRV DWQKKLRERR REVRELKKRQ QKQLERERKM REKQLERERQ ERELQAKLKM EQD SYEMRV AALAPKVEAM EHSWNRIRTI SGADTPEEVL AYWEGLKAKE EQMRSLVSLA EQRESSAKSE IAALLENRSG MYEK GSAAA ADVGEGSEER ATLITEVERN MEGAKGKFNK LRSVCIGAEQ GLRSLQERLM IALEEIHPDQ LRASHMKGGH DAKAR GKGA ASAGARRGSA HAHTPDRNKR GPATGSRSQS PALVPHSPAG DKPSSPLHGT SPEHGHEPIP EGAEELAGEA EMVSPL GAD GNTIDDEHFF PELPELLTSV TDRLNRVLVL AAELDAQEPA GAGEDGLPLS GEPGADGAEG AAPASPSRGA PEGLSES ER TLVKGMNRRT WTGAPLLETI NASPSEAALT LNIKRKKGKK KEQQVQPDLN RILGYTGSDV EEEEPESEEE TEEEANKD D GVVDRDYIKL RALKMSQRLA NQQRAIKV

UniProtKB: Uncharacterized protein

+
Macromolecule #35: DC2

MacromoleculeName: DC2 / type: protein_or_peptide / ID: 35 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 62.292305 KDa
SequenceString: MPSADATRGG GSAGSMGKGT LGAGDTLGHK SVLDKQRAAI EKLRAQNEQL KTELLLENKF SVRPGDPFAQ ALINRLQDEG DMLARKIVL EMRKTKMLDQ QLSEMGSTLT TTRNNMGGIF SAKEQSTAVQ KRIKLLENRL EKAYVKYNQS ITHNKQLRES I NNLRRERI ...String:
MPSADATRGG GSAGSMGKGT LGAGDTLGHK SVLDKQRAAI EKLRAQNEQL KTELLLENKF SVRPGDPFAQ ALINRLQDEG DMLARKIVL EMRKTKMLDQ QLSEMGSTLT TTRNNMGGIF SAKEQSTAVQ KRIKLLENRL EKAYVKYNQS ITHNKQLRES I NNLRRERI MFESIQSNLE RELAKLKRDM ADMIQQANGA FEAREKAIGE MNALKAQADK EQQGFEEEWR QLTTIIEEDK KE RERARAQ ELAMRERETQ ELLKMGTLSS AEKKKRITKG SWNVGYNKAM AQNVAAEKVE MYGQAFKRIQ DATGIEDIDQ LVN TFLAAE DQNYTLFNYV NEVNQEIEKL EDQINIMRGE INKYRETGRE LDMTKSRELT EEEARLAASE AQSQLYEKRT DSAL SMTTA LKAGINDLFE RIGCNTPAVR DLLGEEGVTE ANLTAYLGII EQRTNEILQI YAKRKAQQGT DGLAEALLAQ PLTQP GNRI IIEPPSTTQE EEVEGLEPEP VEEDRPLTRE HLESKVQRTL PRKLETAIKV RPAGADATGG KRGSPTRR

UniProtKB: Outer dynein arm protein 1

+
Macromolecule #36: DC3

MacromoleculeName: DC3 / type: protein_or_peptide / ID: 36 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 21.371311 KDa
SequenceString:
MASAAARERY ARKNEQNELE RAFNTIDSKG DKKIDAEELT ELFLRLGHKP KRGEVEDMIW EVDEDCDGCI SLLEFQTLYT RCRDDKTGY EPRGLFNVVE FVMNDKNNQG FITLEEAMQI MYLRYGRAEL DMQLEQVFGT ADLNSGKILT LTEFLHCLHT N QVKQLLNR VTAKTYKAPP PPQKRK

UniProtKB: Outer dynein arm-docking complex protein DC3

+
Macromolecule #37: FAP68

MacromoleculeName: FAP68 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 35.568691 KDa
SequenceString: MGAANENIHM TDGIRRETMK KETLARERSL AAQSPYMAQV ATYRARNPPL DHSRLMQDPK VQDWASIAGT RRSLATNVPD GGPRVNVNL LKYKRDADFI STTPYDGGPS YNAETCMQNW AEDRRDKHYK SGFHPKELRR STRYDSEYSA RFKPTSADYV G RLTHTYNT ...String:
MGAANENIHM TDGIRRETMK KETLARERSL AAQSPYMAQV ATYRARNPPL DHSRLMQDPK VQDWASIAGT RRSLATNVPD GGPRVNVNL LKYKRDADFI STTPYDGGPS YNAETCMQNW AEDRRDKHYK SGFHPKELRR STRYDSEYSA RFKPTSADYV G RLTHTYNT TSRFEGLTRV GTNGIAAPVL PKRSADTSGE HVFYAKDGYG PTPWMDHTAP TARGRFWVGT APHVAHDTIT HS TLRSEPL EFQQRCPTED ARSKILMGNK PLTHESDRTL RIRDDLVATN TFTRTWRTMY QSDHVDFSRR PATVR

UniProtKB: Uncharacterized protein

+
Macromolecule #38: FAP90

MacromoleculeName: FAP90 / type: protein_or_peptide / ID: 38 / Number of copies: 5 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 27.760064 KDa
SequenceString: MNNNKLDEAA ILAGCKGVFS KTSYITHTGQ EGKAEEYEKK GGHRSAFAGK QLATAPLKEG KTVDVYFTKK HDWISDKDPY VDRIRYKDS NQEKKKGFYT SDFSKRDEFT NTIRTEQWRE QLKGENTHAK KALDMFAEAT GLEASQLRTS RKMEPEVFMY D QVFEKEDP ...String:
MNNNKLDEAA ILAGCKGVFS KTSYITHTGQ EGKAEEYEKK GGHRSAFAGK QLATAPLKEG KTVDVYFTKK HDWISDKDPY VDRIRYKDS NQEKKKGFYT SDFSKRDEFT NTIRTEQWRE QLKGENTHAK KALDMFAEAT GLEASQLRTS RKMEPEVFMY D QVFEKEDP GFDGASRTHR DTKNKTMLSR DRANGEMMTT TALAFQAPDE HHKPEHARKP LVRETFFRKT NVFFPEGCAA DP ST

UniProtKB: Uncharacterized protein

+
Macromolecule #39: GUANOSINE-5'-DIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 39 / Number of copies: 170 / Formula: GDP
Molecular weightTheoretical: 443.201 Da
Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

+
Macromolecule #40: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 40 / Number of copies: 170 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #41: GUANOSINE-5'-TRIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 41 / Number of copies: 170 / Formula: GTP
Molecular weightTheoretical: 523.18 Da
Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

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Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statefilament

-
Sample preparation

BufferpH: 7.4 / Component - Name: HMDEKP
Details: 30 mM HEPES, 5 mM MgSO4, 1 mM DTT, 0.5 mM EGTA, 25 mM KCl, PH 7.4
VitrificationCryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV / Details: blot for 4 seconds before plunging.

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Specialist opticsSpherical aberration corrector: Microscope is equipped with a Cs corrector
Energy filter - Name: GIF Bioquantum / Energy filter - Slit width: 20 eV
Image recordingFilm or detector model: GATAN K2 QUANTUM (4k x 4k) / Detector mode: COUNTING / Digitization - Dimensions - Width: 3838 pixel / Digitization - Dimensions - Height: 3710 pixel / Digitization - Frames/image: 1-30 / Number grids imaged: 6 / Number real images: 8314 / Average exposure time: 9.0 sec. / Average electron dose: 38.9 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsCalibrated defocus max: 3.1 µm / Calibrated defocus min: 1.0 µm / Calibrated magnification: 81000 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 0.01 mm / Nominal defocus max: 2.75 µm / Nominal defocus min: 1.25 µm / Nominal magnification: 81000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

DetailsThe movies were drift-corrected using UCSF MotionCorr2
Particle selectionDetails: doublet microtubules were manually selected
Startup modelType of model: EMDB MAP
EMDB ID:

8537

Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 143765
Initial angle assignmentType: PROJECTION MATCHING
Projection matching processing - Number reference projections: 300
Projection matching processing - Angular sampling: 4.0 degrees
Software - Name: EMAN
Final angle assignmentType: PROJECTION MATCHING / Software - Name: RELION
Final 3D classificationNumber classes: 6 / Software - Name: RELION
FSC plot (resolution estimation)

-
Atomic model buiding 1

RefinementSpace: REAL / Protocol: AB INITIO MODEL / Overall B value: 50
Output model

PDB-6u42:
Natively decorated ciliary doublet microtubule

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