Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structure of the Decorated Ciliary Doublet Microtubule.
Journal, issue, pages	Cell, Vol. 179, Issue 4, Page 909-922.e12, Year 2019
Publish date	Oct 31, 2019
Authors	Meisheng Ma / Mihaela Stoyanova / Griffin Rademacher / Susan K Dutcher / Alan Brown / Rui Zhang /
PubMed Abstract	The axoneme of motile cilia is the largest macromolecular machine of eukaryotic cells. In humans, impaired axoneme function causes a range of ciliopathies. Axoneme assembly, structure, and motility ...The axoneme of motile cilia is the largest macromolecular machine of eukaryotic cells. In humans, impaired axoneme function causes a range of ciliopathies. Axoneme assembly, structure, and motility require a radially arranged set of doublet microtubules, each decorated in repeating patterns with non-tubulin components. We use single-particle cryo-electron microscopy to visualize and build an atomic model of the repeating structure of a native axonemal doublet microtubule, which reveals the identities, positions, repeat lengths, and interactions of 38 associated proteins, including 33 microtubule inner proteins (MIPs). The structure demonstrates how these proteins establish the unique architecture of doublet microtubules, maintain coherent periodicities along the axoneme, and stabilize the microtubules against the repeated mechanical stress induced by ciliary motility. Our work elucidates the architectural principles that underpin the assembly of this large, repetitive eukaryotic structure and provides a molecular basis for understanding the etiology of human ciliopathies.
External links	Cell / PubMed:31668805 / PubMed Central
Methods	EM (single particle)
Resolution	3.4 - 4.0 Å
Structure data	20631 6u42 EMDB-20631: Composite cryo-EM density map of the 48-nm repeat doublet microtubule from Chlamydomonas reinhardtii PDB-6u42: Natively decorated ciliary doublet microtubule Method: EM (single particle) / Resolution: 3.4 Å EMDB-20632: Composite cryo-EM density map of half 1 of the 96-nm repeat of the doublet microtubule from wild-type Chlamydomonas reinhardtii Method: EM (single particle) / Resolution: 3.6 Å EMDB-20633: Composite cryo-EM density map of half 2 of the 96-nm repeat of the doublet microtubule from wild-type Chlamydomonas reinhardtii Method: EM (single particle) / Resolution: 3.57 Å EMDB-20634: Composite cryo-EM density map of protofilaments A07-A08 of the 48-nm repeat doublet microtubule (extended lattice) Method: EM (single particle) / Resolution: 3.45 Å EMDB-20635: Composite cryo-EM density map of protofilaments A07-A08 of the 48-nm repeat doublet microtubule (compact lattice) Method: EM (single particle) / Resolution: 3.66 Å EMDB-20636: Composite cryo-EM density map of the 48-nm repeat of the doublet microtubule from Chlamydomonas reinhardtii rib72 mutant Method: EM (single particle) / Resolution: 3.9 Å EMDB-20637: Composite cryo-EM density map of the 48-nm repeat of the doublet microtubule from Chlamydomonas reinhardtii fap166 mutant Method: EM (single particle) / Resolution: 4.0 Å
Chemicals	ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying moleculeYM / Guanosine diphosphate ChemComp-MG: Unknown entry ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying moleculeYM / Guanosine triphosphate
Source	chlamydomonas reinhardtii (plant)
Keywords	PROTEIN FIBRIL / motile cilia / microtubule doublet / repetitive structure