EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of the Decorated Ciliary Doublet Microtubule.
ジャーナル・号・ページ	Cell, Vol. 179, Issue 4, Page 909-922.e12, Year 2019
掲載日	2019年10月31日
著者	Meisheng Ma / Mihaela Stoyanova / Griffin Rademacher / Susan K Dutcher / Alan Brown / Rui Zhang /
PubMed 要旨	The axoneme of motile cilia is the largest macromolecular machine of eukaryotic cells. In humans, impaired axoneme function causes a range of ciliopathies. Axoneme assembly, structure, and motility ...The axoneme of motile cilia is the largest macromolecular machine of eukaryotic cells. In humans, impaired axoneme function causes a range of ciliopathies. Axoneme assembly, structure, and motility require a radially arranged set of doublet microtubules, each decorated in repeating patterns with non-tubulin components. We use single-particle cryo-electron microscopy to visualize and build an atomic model of the repeating structure of a native axonemal doublet microtubule, which reveals the identities, positions, repeat lengths, and interactions of 38 associated proteins, including 33 microtubule inner proteins (MIPs). The structure demonstrates how these proteins establish the unique architecture of doublet microtubules, maintain coherent periodicities along the axoneme, and stabilize the microtubules against the repeated mechanical stress induced by ciliary motility. Our work elucidates the architectural principles that underpin the assembly of this large, repetitive eukaryotic structure and provides a molecular basis for understanding the etiology of human ciliopathies.
リンク	Cell / PubMed:31668805 / PubMed Central
手法	EM (単粒子)
解像度	3.4 - 4.0 Å
構造データ	20631 6u42 EMDB-20631: Composite cryo-EM density map of the 48-nm repeat doublet microtubule from Chlamydomonas reinhardtii PDB-6u42: Natively decorated ciliary doublet microtubule 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-20632: Composite cryo-EM density map of half 1 of the 96-nm repeat of the doublet microtubule from wild-type Chlamydomonas reinhardtii 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-20633: Composite cryo-EM density map of half 2 of the 96-nm repeat of the doublet microtubule from wild-type Chlamydomonas reinhardtii 手法: EM (単粒子) / 解像度: 3.57 Å EMDB-20634: Composite cryo-EM density map of protofilaments A07-A08 of the 48-nm repeat doublet microtubule (extended lattice) 手法: EM (単粒子) / 解像度: 3.45 Å EMDB-20635: Composite cryo-EM density map of protofilaments A07-A08 of the 48-nm repeat doublet microtubule (compact lattice) 手法: EM (単粒子) / 解像度: 3.66 Å EMDB-20636: Composite cryo-EM density map of the 48-nm repeat of the doublet microtubule from Chlamydomonas reinhardtii rib72 mutant 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-20637: Composite cryo-EM density map of the 48-nm repeat of the doublet microtubule from Chlamydomonas reinhardtii fap166 mutant 手法: EM (単粒子) / 解像度: 4.0 Å
化合物	ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM / グアノシン二リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM / グアノシン三リン酸
由来	chlamydomonas reinhardtii (クラミドモナス)
キーワード	PROTEIN FIBRIL / motile cilia / microtubule doublet / repetitive structure