- EMDB-22418: ORC-O2WH: Human Origin Recognition Complex (ORC) with dynamic/unr... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-22418
タイトル
ORC-O2WH: Human Origin Recognition Complex (ORC) with dynamic/unresolved ORC1 AAA+ domain
マップデータ
Human Origin Recognition Complex (ORC) with dynamic/unresolved ORC1 AAA+ domain
試料
複合体: ORC-O1AAA
タンパク質・ペプチド: Origin recognition complex subunit 1
タンパク質・ペプチド: Origin recognition complex subunit 2
タンパク質・ペプチド: Origin recognition complex subunit 3
タンパク質・ペプチド: Origin recognition complex subunit 4
タンパク質・ペプチド: Origin recognition complex subunit 5
リガンド: ADENOSINE-5'-TRIPHOSPHATE
リガンド: MAGNESIUM ION
キーワード
replication / AAA+ / ORC / DNA-binding / cryoEM
機能・相同性
機能・相同性情報
polar body extrusion after meiotic divisions / CDC6 association with the ORC:origin complex / origin recognition complex / E2F-enabled inhibition of pre-replication complex formation / nuclear origin of replication recognition complex / nuclear pre-replicative complex / inner kinetochore / DNA replication preinitiation complex / neural precursor cell proliferation / mitotic DNA replication checkpoint signaling ...polar body extrusion after meiotic divisions / CDC6 association with the ORC:origin complex / origin recognition complex / E2F-enabled inhibition of pre-replication complex formation / nuclear origin of replication recognition complex / nuclear pre-replicative complex / inner kinetochore / DNA replication preinitiation complex / neural precursor cell proliferation / mitotic DNA replication checkpoint signaling / G1/S-Specific Transcription / regulation of DNA replication / DNA replication origin binding / protein polymerization / Activation of the pre-replicative complex / DNA replication initiation / glial cell proliferation / heterochromatin / Activation of ATR in response to replication stress / Assembly of the ORC complex at the origin of replication / Assembly of the pre-replicative complex / Orc1 removal from chromatin / DNA replication / chromosome, telomeric region / nuclear body / nucleotide binding / centrosome / chromatin binding / chromatin / nucleolus / negative regulation of transcription by RNA polymerase II / ATP hydrolysis activity / DNA binding / nucleoplasm / ATP binding / membrane / nucleus / metal ion binding / cytosol 類似検索 - 分子機能
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
F32GM129923
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM45436
米国
引用
ジャーナル: Elife / 年: 2020 タイトル: The dynamic nature of the human origin recognition complex revealed through five cryoEM structures. 著者: Matt J Jaremko / Kin Fan On / Dennis R Thomas / Bruce Stillman / Leemor Joshua-Tor / 要旨: Genome replication is initiated from specific origin sites established by dynamic events. The Origin Recognition Complex (ORC) is necessary for orchestrating the initiation process by binding to ...Genome replication is initiated from specific origin sites established by dynamic events. The Origin Recognition Complex (ORC) is necessary for orchestrating the initiation process by binding to origin DNA, recruiting CDC6, and assembling the MCM replicative helicase on DNA. Here we report five cryoEM structures of the human ORC (HsORC) that illustrate the native flexibility of the complex. The absence of ORC1 revealed a compact, stable complex of ORC2-5. Introduction of ORC1 opens the complex into several dynamic conformations. Two structures revealed dynamic movements of the ORC1 AAA+ and ORC2 winged-helix domains that likely impact DNA incorporation into the ORC core. Additional twist and pinch motions were observed in an open ORC conformation revealing a hinge at the ORC5·ORC3 interface that may facilitate ORC binding to DNA. Finally, a structure of ORC was determined with endogenous DNA bound in the core revealing important differences between human and yeast origin recognition.
タイプ: ANGULAR RECONSTITUTION / ソフトウェア - 名称: cryoSPARC (ver. 2) 詳細: Utilized Cryosparc to generate initial model, then 3D classification in Relion using the Cryosparc initial model