EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Conformational changes of the baseplate regulating tail contraction of Staphylococcus phage 812.
ジャーナル・号・ページ	EMBO J, Vol. 45, Issue 15, Page 5514-5536, Year 2026
掲載日	2026年6月26日
著者	Ján Bíňovský / Marta Šiborová / Maryna Zlatohurska / Jiří Nováček / Pavol Bárdy / Roman Baška / Karel Škubník / Tibor Botka / Martin Benešík / Roman Pantůček / Konstantinos Tripsianes / Pavel Plevka /
PubMed 要旨	Phages with contractile tails employ elaborate mechanisms to penetrate bacterial cell walls and deliver their genomes into the host cytoplasm. Here, we used cryo-EM to show that the baseplate of ...Phages with contractile tails employ elaborate mechanisms to penetrate bacterial cell walls and deliver their genomes into the host cytoplasm. Here, we used cryo-EM to show that the baseplate of phage 812, a member of the Kayvirus genus, which infects Gram-positive Staphylococcus strains, is formed of a core, wedge modules, and baseplate arms carrying receptor-binding proteins 1 and 2 and tripod complexes. Upon binding to a host cell, the receptor-binding proteins of phage 812 baseplate reorient and undergo conformational changes. The changes to the tripod complexes trigger the release of the central spike and weld proteins, which expose peptidoglycan-degrading domains of the hub proteins. Changes in the positions of baseplate arms are transmitted through wedge modules to tail sheath initiator proteins. The ring of the tail sheath initiator proteins expands and triggers the contraction of the tail sheath, which shortens to 50% and pushes the tail tube 10-30 nm into the bacterial cytoplasm. Homologous molecular mechanisms are probably shared by phages of the Herelleviridae family with contractile tails to infect Gram-positive bacteria.
リンク	EMBO J / PubMed:42350675 / PubMed Central
手法	EM (単粒子) / NMR (溶液) / X線回折
解像度	1.8 - 8.4 Å
構造データ	19972 9euj EMDB-19972, PDB-9euj: Cryo-EM structure of Staphylococcus aureus bacteriophage phi812 baseplate in the post-contraction state - sheath initiator, wedge module, inner tripod, arm segment, and proximal tail sheath proteins 手法: EM (単粒子) / 解像度: 4.0 Å 19973 9euk EMDB-19973, PDB-9euk: Cryo-EM structure of Staphylococcus aureus bacteriophage phi812 baseplate in the post-contraction state - sheath initiator, wedge module, and inner tripod proteins 手法: EM (単粒子) / 解像度: 3.1 Å 19974 9eul EMDB-19974, PDB-9eul: Cryo-EM structure of Staphylococcus aureus bacteriophage phi812 central spike protein - knob and petal domains 手法: EM (単粒子) / 解像度: 3.23 Å 50093 9f04 EMDB-50093, PDB-9f04: Cryo-EM structure of Staphylococcus aureus bacteriophage phi812 tail in the pre-contraction state - tube and sheath proteins 手法: EM (単粒子) / 解像度: 4.1 Å 50094 9f05 EMDB-50094, PDB-9f05: Cryo-EM structure of Staphylococcus aureus bacteriophage phi812 tail in the post-contraction state - sheath proteins 手法: EM (単粒子) / 解像度: 3.0 Å 50095 9f06 EMDB-50095, PDB-9f06: Cryo-EM structure of Staphylococcus aureus bacteriophage phi812 tail in the post-contraction state - tube proteins 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-55950: Phage 812 baseplate in the pre-contraction state (C1) 手法: EM (単粒子) / 解像度: 8.4 Å 55951 9tic EMDB-55951, PDB-9tic: Phage 812 baseplate in the pre-contraction state (C3) 手法: EM (単粒子) / 解像度: 5.9 Å 55952 9tid EMDB-55952, PDB-9tid: Phage 812 baseplate in the pre-contraction state - core and wedge module proteins 手法: EM (単粒子) / 解像度: 3.0 Å 55953 9tie EMDB-55953, PDB-9tie: Phage 812 baseplate in the pre-contraction state - tail sheath initiator and baseplate-proximal tail proteins 手法: EM (単粒子) / 解像度: 3.4 Å 55954 9tif EMDB-55954, PDB-9tif: Phage 812 baseplate in the pre-contraction state - lower arm 手法: EM (単粒子) / 解像度: 5.6 Å 55955 9tig EMDB-55955, PDB-9tig: Phage 812 baseplate in the pre-contraction state - lower arm (segment A) 手法: EM (単粒子) / 解像度: 4.0 Å 55956 9tih EMDB-55956, PDB-9tih: Phage 812 baseplate in the pre-contraction state - lower arm (segment B) 手法: EM (単粒子) / 解像度: 4.0 Å 55957 9tii EMDB-55957, PDB-9tii: Phage 812 baseplate in the pre-contraction state - lower arm (segment C) 手法: EM (単粒子) / 解像度: 6.0 Å 55958 9tij EMDB-55958, PDB-9tij: Phage 812 baseplate in the pre-contraction state - lower arm (segment DEF) 手法: EM (単粒子) / 解像度: 6.3 Å 55959 9tik EMDB-55959, PDB-9tik: Phage 812 baseplate in the pre-contraction state - lower arm (uRBP1-lRBP2) 手法: EM (単粒子) / 解像度: 4.9 Å 55960 9til EMDB-55960, PDB-9til: Phage 812 baseplate in the pre-contraction state - lower arm (lRBP1-uRBP2) 手法: EM (単粒子) / 解像度: 4.1 Å 55961 9tim EMDB-55961, PDB-9tim: Phage 812 baseplate in the pre-contraction state - upper arm 手法: EM (単粒子) / 解像度: 5.1 Å 55962 9tin EMDB-55962, PDB-9tin: Phage 812 baseplate in the pre-contraction state - upper arm (segment A) 手法: EM (単粒子) / 解像度: 3.8 Å 55963 9tio EMDB-55963, PDB-9tio: Phage 812 baseplate in the pre-contraction state - upper arm (segment B) 手法: EM (単粒子) / 解像度: 3.9 Å 55964 9tip EMDB-55964, PDB-9tip: Phage 812 baseplate in the pre-contraction state - upper arm (segment CDEF) 手法: EM (単粒子) / 解像度: 5.2 Å EMDB-55966: Phage 812 baseplate in the post-contraction state (C1) 手法: EM (単粒子) / 解像度: 6.6 Å 55967 9tir EMDB-55967, PDB-9tir: Phage 812 baseplate in the post-contraction state (C6) 手法: EM (単粒子) / 解像度: 4.4 Å 55968 9tis EMDB-55968, PDB-9tis: Baseplate arm of phage 812 in the post-contraction state 手法: EM (単粒子) / 解像度: 4.1 Å 55969 9tit EMDB-55969, PDB-9tit: Baseplate arm (segment B) of phage 812 in the post-contraction state 手法: EM (単粒子) / 解像度: 4.4 Å EMDB-55977: Phage 812 baseplate in the pre-contraction state - composite 手法: EM (単粒子) / 解像度: 6.0 Å 55978 9tiw EMDB-55978, PDB-9tiw: Phage 812 baseplate in the post-contraction state - composite 手法: EM (単粒子) / 解像度: 4.4 Å PDB-9eum: NMR structure of the Staphylococcus aureus bacteriophage phi812 hub protein - lytic cleaver (CHAP) domain 手法: SOLUTION NMR PDB-9fko: Arm segment of bacteriophage phi812 手法: X-RAY DIFFRACTION / 解像度: 1.8 Å
化合物	ChemComp-HOH: WATER
由来	staphylococcus phage 812 (ファージ) staphylococcus phage 812k1/420 (ファージ) escherichia coli (大腸菌)
キーワード	VIRUS / bacteriophage / phage / contractile / phi812 / baseplate / VIRAL PROTEIN / spike / central spike / TIM / HYDROLASE / CHAP / peptidase / peptidoglycan / tail / arm segment