Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Conformational changes of the baseplate regulating tail contraction of Staphylococcus phage 812.
Journal, issue, pages	Embo J., Year 2026
Publish date	Dec 5, 2025
Authors	Ján Bíňovský / Marta Šiborová / Maryna Zlatohurska / Jiří Nováček / Pavol Bárdy / Roman Baška / Karel Škubník / Tibor Botka / Martin Benešík / Roman Pantůček / Konstantinos Tripsianes / Pavel Plevka /
PubMed Abstract	Phages with contractile tails employ elaborate mechanisms to penetrate bacterial cell walls and deliver their genomes into the host cytoplasm. Here, we used cryo-EM to show that the baseplate of ...Phages with contractile tails employ elaborate mechanisms to penetrate bacterial cell walls and deliver their genomes into the host cytoplasm. Here, we used cryo-EM to show that the baseplate of phage 812, a member of the Kayvirus genus, which infects Gram-positive Staphylococcus strains, is formed of a core, wedge modules, and baseplate arms carrying receptor-binding proteins 1 and 2 and tripod complexes. Upon binding to a host cell, the receptor-binding proteins of phage 812 baseplate reorient and undergo conformational changes. The changes to the tripod complexes trigger the release of the central spike and weld proteins, which expose peptidoglycan-degrading domains of the hub proteins. Changes in the positions of baseplate arms are transmitted through wedge modules to tail sheath initiator proteins. The ring of the tail sheath initiator proteins expands and triggers the contraction of the tail sheath, which shortens to 50% and pushes the tail tube 10-30 nm into the bacterial cytoplasm. Homologous molecular mechanisms are probably shared by phages of the Herelleviridae family with contractile tails to infect Gram-positive bacteria.
External links	Embo J. / PubMed:42350675
Methods	EM (single particle) / NMR (solution) / X-ray diffraction
Resolution	1.8 - 8.4 Å
Structure data	19972 9euj EMDB-19972, PDB-9euj: Cryo-EM structure of Staphylococcus aureus bacteriophage phi812 baseplate in the post-contraction state - sheath initiator, wedge module, inner tripod, arm segment, and proximal tail sheath proteins Method: EM (single particle) / Resolution: 4.0 Å 19973 9euk EMDB-19973, PDB-9euk: Cryo-EM structure of Staphylococcus aureus bacteriophage phi812 baseplate in the post-contraction state - sheath initiator, wedge module, and inner tripod proteins Method: EM (single particle) / Resolution: 3.1 Å 19974 9eul EMDB-19974, PDB-9eul: Cryo-EM structure of Staphylococcus aureus bacteriophage phi812 central spike protein - knob and petal domains Method: EM (single particle) / Resolution: 3.23 Å 50093 9f04 EMDB-50093, PDB-9f04: Cryo-EM structure of Staphylococcus aureus bacteriophage phi812 tail in the pre-contraction state - tube and sheath proteins Method: EM (single particle) / Resolution: 4.1 Å 50094 9f05 EMDB-50094, PDB-9f05: Cryo-EM structure of Staphylococcus aureus bacteriophage phi812 tail in the post-contraction state - sheath proteins Method: EM (single particle) / Resolution: 3.0 Å 50095 9f06 EMDB-50095, PDB-9f06: Cryo-EM structure of Staphylococcus aureus bacteriophage phi812 tail in the post-contraction state - tube proteins Method: EM (single particle) / Resolution: 3.6 Å EMDB-55950: Phage 812 baseplate in the pre-contraction state (C1) Method: EM (single particle) / Resolution: 8.4 Å 55951 9tic EMDB-55951, PDB-9tic: Phage 812 baseplate in the pre-contraction state (C3) Method: EM (single particle) / Resolution: 5.9 Å 55952 9tid EMDB-55952, PDB-9tid: Phage 812 baseplate in the pre-contraction state - core and wedge module proteins Method: EM (single particle) / Resolution: 3.0 Å 55953 9tie EMDB-55953, PDB-9tie: Phage 812 baseplate in the pre-contraction state - tail sheath initiator and baseplate-proximal tail proteins Method: EM (single particle) / Resolution: 3.4 Å 55954 9tif EMDB-55954, PDB-9tif: Phage 812 baseplate in the pre-contraction state - lower arm Method: EM (single particle) / Resolution: 5.6 Å 55955 9tig EMDB-55955, PDB-9tig: Phage 812 baseplate in the pre-contraction state - lower arm (segment A) Method: EM (single particle) / Resolution: 4.0 Å 55956 9tih EMDB-55956, PDB-9tih: Phage 812 baseplate in the pre-contraction state - lower arm (segment B) Method: EM (single particle) / Resolution: 4.0 Å 55957 9tii EMDB-55957, PDB-9tii: Phage 812 baseplate in the pre-contraction state - lower arm (segment C) Method: EM (single particle) / Resolution: 6.0 Å 55958 9tij EMDB-55958, PDB-9tij: Phage 812 baseplate in the pre-contraction state - lower arm (segment DEF) Method: EM (single particle) / Resolution: 6.3 Å 55959 9tik EMDB-55959, PDB-9tik: Phage 812 baseplate in the pre-contraction state - lower arm (uRBP1-lRBP2) Method: EM (single particle) / Resolution: 4.9 Å 55960 9til EMDB-55960, PDB-9til: Phage 812 baseplate in the pre-contraction state - lower arm (lRBP1-uRBP2) Method: EM (single particle) / Resolution: 4.1 Å 55961 9tim EMDB-55961, PDB-9tim: Phage 812 baseplate in the pre-contraction state - upper arm Method: EM (single particle) / Resolution: 5.1 Å 55962 9tin EMDB-55962, PDB-9tin: Phage 812 baseplate in the pre-contraction state - upper arm (segment A) Method: EM (single particle) / Resolution: 3.8 Å 55963 9tio EMDB-55963, PDB-9tio: Phage 812 baseplate in the pre-contraction state - upper arm (segment B) Method: EM (single particle) / Resolution: 3.9 Å 55964 9tip EMDB-55964, PDB-9tip: Phage 812 baseplate in the pre-contraction state - upper arm (segment CDEF) Method: EM (single particle) / Resolution: 5.2 Å EMDB-55966: Phage 812 baseplate in the post-contraction state (C1) Method: EM (single particle) / Resolution: 6.6 Å 55967 9tir EMDB-55967, PDB-9tir: Phage 812 baseplate in the post-contraction state (C6) Method: EM (single particle) / Resolution: 4.4 Å 55968 9tis EMDB-55968, PDB-9tis: Baseplate arm of phage 812 in the post-contraction state Method: EM (single particle) / Resolution: 4.1 Å 55969 9tit EMDB-55969, PDB-9tit: Baseplate arm (segment B) of phage 812 in the post-contraction state Method: EM (single particle) / Resolution: 4.4 Å EMDB-55977: Phage 812 baseplate in the pre-contraction state - composite Method: EM (single particle) / Resolution: 6.0 Å 55978 9tiw EMDB-55978, PDB-9tiw: Phage 812 baseplate in the post-contraction state - composite Method: EM (single particle) / Resolution: 4.4 Å PDB-9eum: NMR structure of the Staphylococcus aureus bacteriophage phi812 hub protein - lytic cleaver (CHAP) domain Method: SOLUTION NMR PDB-9fko: Arm segment of bacteriophage phi812 Method: X-RAY DIFFRACTION / Resolution: 1.8 Å
Chemicals	ChemComp-HOH: WATER
Source	staphylococcus phage 812 (virus) staphylococcus phage 812k1/420 (virus) escherichia coli (E. coli)
Keywords	VIRUS / bacteriophage / phage / contractile / phi812 / baseplate / VIRAL PROTEIN / spike / central spike / TIM / HYDROLASE / CHAP / peptidase / peptidoglycan / tail / arm segment