Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug.
Journal, issue, pages	Nat Struct Mol Biol, Vol. 32, Issue 11, Page 2158-2164, Year 2025
Publish date	Aug 18, 2025
Authors	Leonie Anton / Wenjing Cheng / Meseret T Haile / Jerzy M Dziekan / David W Cobb / Xiyan Zhu / Leyan Han / Emerson Li / Anjali Nair / Carolyn L Lee / Hanyu Wang / Hangjun Ke / Guoan Zhang / Emma H Doud / Alan F Cowman / Chi-Min Ho /
PubMed Abstract	Our understanding of cellular events is hampered by the gap between the resolution at which we can observe events inside cells and our ability to replicate physiological conditions in test tubes. ...Our understanding of cellular events is hampered by the gap between the resolution at which we can observe events inside cells and our ability to replicate physiological conditions in test tubes. Here, we show in Plasmodium falciparum, a non-model organism of high medical importance, that this gap can be bridged by using an integrated structural biology approach to visualize events inside the cell at molecular resolution. We determined eight high-resolution structures of the native malarial ribosome in actively translating states inside P. falciparum-infected human erythrocytes using in situ cryo-electron tomography. Following perturbation with a Plasmodium-specific translation inhibitor, we then observed a decrease in elongation factor-bound ribosomal states and an apparent upregulation of ribosome biogenesis in inhibitor-treated parasites. Our work elucidates new molecular details of the malarial translation elongation cycle and demonstrates direct multiscale visualization of drug-induced phenotypic changes in the structure and localization of individual molecules within the native cellular context.
External links	Nat Struct Mol Biol / PubMed:40825879 / PubMed Central
Methods	EM (subtomogram averaging) / EM (single particle)
Resolution	2.3 - 18.0 Å
Structure data	41485 8tpu EMDB-41485, PDB-8tpu: Subtomogram averaged consensus structure of the malarial 80S ribosome in Plasmodium falciparum-infected human erythrocytes Method: EM (subtomogram averaging) / Resolution: 4.1 Å EMDB-41486: Subtomogram averaged decoding-1 state of the malarial 80S ribosome in Plasmodium falciparum-infected human erythrocytes Method: EM (subtomogram averaging) / Resolution: 6.8 Å EMDB-41487: Subtomogram averaged decoding-2 state of the malarial 80S ribosome in Plasmodium falciparum-infected human erythrocytes Method: EM (subtomogram averaging) / Resolution: 18.0 Å EMDB-41488: Subtomogram averaged classical iPRE state of the malarial 80S ribosome in Plasmodium falciparum-infected human erythrocytes Method: EM (subtomogram averaging) / Resolution: 5.2 Å EMDB-41489: Subtomogram averaged rotated-1 PRE state of the malarial 80S ribosome in Plasmodium falciparum-infected human erythrocytes Method: EM (subtomogram averaging) / Resolution: 10.2 Å EMDB-41490: Subtomogram averaged rotated-2 PRE state of the malarial 80S ribosome in Plasmodium falciparum-infected human erythrocytes Method: EM (subtomogram averaging) / Resolution: 6.6 Å EMDB-41491: Subtomogram averaged translocation state of the malarial 80S ribosome in Plasmodium falciparum-infected human erythrocytes Method: EM (subtomogram averaging) / Resolution: 6.5 Å EMDB-41492: Subtomogram averaged POST state of the malarial 80S ribosome in Plasmodium falciparum-infected human erythrocytes Method: EM (subtomogram averaging) / Resolution: 4.6 Å EMDB-41493: Subtomogram averaged unloaded state of the malarial 80S ribosome in Plasmodium falciparum-infected human erythrocytes Method: EM (subtomogram averaging) / Resolution: 4.6 Å EMDB-41494: Subtomogram averaged non-rotated 80S ribosome of the malarial 80S ribosome in Plasmodium falciparum-infected human erythrocytes Method: EM (subtomogram averaging) / Resolution: 4.3 Å EMDB-42209: Subtomogram averaged rotated-1 PRE back rolled state of the malarial 80S ribosome in Plasmodium falciparum-infected human erythrocytes Method: EM (subtomogram averaging) / Resolution: 10.9 Å EMDB-42210: Subtomogram averaged eEF1alpha hibernating state of the malarial 80S ribosome in Plasmodium falciparum-infected human erythrocytes Method: EM (subtomogram averaging) / Resolution: 12.2 Å EMDB-42211: Subtomogram averaged eEF2 hibernating-1 state of the malarial 80S ribosome in Plasmodium falciparum-infected human erythrocytes Method: EM (subtomogram averaging) / Resolution: 11.4 Å 44915 9bup EMDB-44915, PDB-9bup: Single particle CryoEM structure of the Pf80S ribosome in non-rotated PRE state (nrt A-P-E) Method: EM (single particle) / Resolution: 2.7 Å 44916 9buq EMDB-44916, PDB-9buq: Single particle cryoEM structure of the Pf80S ribosome in the POST state (nrt with P- and E-site tRNA) Method: EM (single particle) / Resolution: 2.3 Å 44918 9bus EMDB-44918, PDB-9bus: Single particle CryoEM structure of the Pf80S ribosome in the unloaded state (nrt with E-site tRNA) Method: EM (single particle) / Resolution: 2.4 Å 44919 9but EMDB-44919, PDB-9but: Single particle CryoEM structure of the Pf80S ribosome in the rotated-2 PRE state (rt state with P and E-site tRNA) Method: EM (single particle) / Resolution: 2.8 Å 44920 9buu EMDB-44920, PDB-9buu: Single particle CryoEM structure of the Pf80S ribosome in rotated state with E-site tRNA Method: EM (single particle) / Resolution: 2.8 Å
Source	plasmodium falciparum 3d7 (eukaryote)
Keywords	RIBOSOME / malaria / cryoET / in situ / Plasmodium falciparum / translation / cryoEM / single particle