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- EMDB-41492: Subtomogram averaged POST state of the malarial 80S ribosome in P... -
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Open data
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Basic information
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Title | Subtomogram averaged POST state of the malarial 80S ribosome in Plasmodium falciparum-infected human erythrocytes | ||||||||||||
![]() | Plasmodium falciparum POST state postprocessing | ||||||||||||
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![]() | ribosome / malaria / cryoET / in situ / Plasmodium falciparum / TRANSLATION | ||||||||||||
Biological species | ![]() ![]() | ||||||||||||
Method | subtomogram averaging / cryo EM / Resolution: 4.6 Å | ||||||||||||
![]() | Anton L / Cheng W / Zhu X / Ho CM | ||||||||||||
Funding support | ![]() ![]() ![]()
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![]() | ![]() Title: Divergent translational landscape reflects adaptation to biased codon usage in malaria parasites Authors: Anton L / Cheng W / Haile M / Cobb DW / Zhu X / Han L / Li E / Nair A / Lee CL / Ho CM | ||||||||||||
History |
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Structure visualization
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Downloads & links
-EMDB archive
Map data | ![]() | |||
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Header (meta data) | ![]() | |||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 41485 ![]() 41486 ![]() 41487 ![]() 41488 ![]() 41489 ![]() 41490 ![]() 41491 ![]() 41493 ![]() 41494 ![]() 8tpuC C: citing same article ( |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Plasmodium falciparum POST state postprocessing | ||||||||||||||||||||
Voxel size | X=Y=Z: 2.094 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
-Entire : Plasmodium falciparum 80S ribosome consensus structure
Entire | Name: Plasmodium falciparum 80S ribosome consensus structure |
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Components |
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-Supramolecule #1: Plasmodium falciparum 80S ribosome consensus structure
Supramolecule | Name: Plasmodium falciparum 80S ribosome consensus structure type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#79 Details: Subtomogram averaged consensus structure of the malarial 80S ribosome in Plasmodium falciparum-infected human erythrocytes |
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Source (natural) | Organism: ![]() ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | subtomogram averaging |
Aggregation state | cell |
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Sample preparation
Buffer | pH: 7.2 |
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Vitrification | Cryogen name: ETHANE-PROPANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 2.93 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.2 µm / Nominal defocus min: 2.8000000000000003 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 4.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number subtomograms used: 15917 |
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Extraction | Number tomograms: 848 / Number images used: 129617 |
Final angle assignment | Type: OTHER |