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TitleClpS Directs Degradation of N-Degron Substrates With Primary Destabilizing Residues in Mycolicibacterium smegmatis.
Journal, issue, pagesMol. Microbiol., Vol. 123, Page 16-30, Year 2025
Publish dateMar 8, 2024 (structure data deposition date)
AuthorsPresloid, C.J. / Jiang, J. / Kandel, P. / Anderson, H.R. / Beardslee, P.C. / Swayne, T.M. / Schmitz, K.R.
External linksMol. Microbiol. / PubMed:39626090
MethodsX-ray diffraction
Resolution0.97 - 1.99 Å
Structure data

PDB-9ayn:
Mycolicibacterium smegmatis ClpS with bound Ni2+
Method: X-RAY DIFFRACTION / Resolution: 0.97 Å

PDB-9ayo:
Mycolicibacterium smegmatis ClpS with bound PheAla and Ni2+
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

PDB-9ayp:
Mycolicibacterium smegmatis ClpS with bound Co2+
Method: X-RAY DIFFRACTION / Resolution: 1.99 Å

PDB-9az5:
Mycolicibacterium smegmatis ClpS with bound Mg2+
Method: X-RAY DIFFRACTION / Resolution: 1.47 Å

PDB-9b06:
Mycolicibacterium smegmatis ClpS with LeuThr dipeptide and Mg2+
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

PDB-9b10:
Mycolicibacterium smegmatis ClpS with TyrArg dipeptide and Mg2+
Method: X-RAY DIFFRACTION / Resolution: 1.28 Å

PDB-9b1p:
Mycolicibacterium smegmatis ClpS with TrpSer dipeptide and Mg2+
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

Chemicals

ChemComp-NI:
NICKEL (II) ION

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-PHE:
PHENYLALANINE

ChemComp-ALA:
ALANINE

ChemComp-CO:
Unknown entry

ChemComp-DMS:
DIMETHYL SULFOXIDE / DMSO, precipitant*YM

ChemComp-LCZ:
(2S)-2-amino-4-methylpentanal

ChemComp-THR:
THREONINE

ChemComp-TYR:
TYROSINE

ChemComp-ARG:
ARGININE

ChemComp-GOL:
GLYCEROL

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-PGE:
TRIETHYLENE GLYCOL

Source
  • mycolicibacterium smegmatis mc2 155 (bacteria)
KeywordsPROTEIN BINDING / proteolysis / adaptor

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