[English] 日本語
Yorodumi Papers
- Database of articles cited by EMDB/PDB/SASBDB data -

+
Search query

Keywords
Structure methods
Author
Journal
IF

-
Structure paper

TitleStructure of the human inner kinetochore bound to a centromeric CENP-A nucleosome.
Journal, issue, pagesScience, Vol. 376, Issue 6595, Page 844-852, Year 2022
Publish dateMay 20, 2022
AuthorsStanislau Yatskevich / Kyle W Muir / Dom Bellini / Ziguo Zhang / Jing Yang / Thomas Tischer / Masa Predin / Tom Dendooven / Stephen H McLaughlin / David Barford /
PubMed AbstractKinetochores assemble onto specialized centromeric CENP-A (centromere protein A) nucleosomes (CENP-A) to mediate attachments between chromosomes and the mitotic spindle. We describe cryo-electron ...Kinetochores assemble onto specialized centromeric CENP-A (centromere protein A) nucleosomes (CENP-A) to mediate attachments between chromosomes and the mitotic spindle. We describe cryo-electron microscopy structures of the human inner kinetochore constitutive centromere associated network (CCAN) complex bound to CENP-A reconstituted onto α-satellite DNA. CCAN forms edge-on contacts with CENP-A, and a linker DNA segment of the α-satellite repeat emerges from the fully wrapped end of the nucleosome to thread through the central CENP-LN channel that tightly grips the DNA. The CENP-TWSX histone-fold module further augments DNA binding and partially wraps the linker DNA in a manner reminiscent of canonical nucleosomes. Our study suggests that the topological entrapment of the linker DNA by CCAN provides a robust mechanism by which kinetochores withstand both pushing and pulling forces exerted by the mitotic spindle.
External linksScience / PubMed:35420891 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution2.44 - 12.0 Å
Structure data

13437

7pii

EMDB-13437, PDB-7pii:
Structure of the human CCAN CENP-A alpha-satellite complex
Method: EM (single particle) / Resolution: 2.68 Å

13473

7pkn

EMDB-13473, PDB-7pkn:
Structure of the human CCAN deltaCT complex
Method: EM (single particle) / Resolution: 3.2 Å

14334

7r5r

EMDB-14334, PDB-7r5r:
Structure of the human CCAN CENP-A alpha-satellite complex
Method: EM (single particle) / Resolution: 2.44 Å

14336

7r5s

EMDB-14336, PDB-7r5s:
Structure of the human CCAN bound to alpha satellite DNA
Method: EM (single particle) / Resolution: 2.83 Å

14341

7r5v

EMDB-14341, PDB-7r5v:
Structure of the human CCAN CENP-A alpha-satellite complex
Method: EM (single particle) / Resolution: 4.55 Å

14351

7ywx

EMDB-14351, PDB-7ywx:
Structure of the human CCAN CENP-A alpha-satellite complex
Method: EM (single particle) / Resolution: 12.0 Å

14375

7yyh

EMDB-14375, PDB-7yyh:
Structure of the human CCANdeltaT CENP-A alpha-satellite complex
Method: EM (single particle) / Resolution: 8.9 Å

PDB-7pb4:
Cenp-HIK 3-protein complex
Method: X-RAY DIFFRACTION / Resolution: 2.49 Å

PDB-7pb8:
Crystal structure of the CENP-OPQUR complex
Method: X-RAY DIFFRACTION / Resolution: 3.68 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
KeywordsCELL CYCLE / inner kinetochore / Kinetochore / chromosome segregation / centromere / Chromosome / cell division

+
About Yorodumi Papers

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB Accession Codes are Changing Soon! / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary.
  • This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

-
Yorodumi Papers

Database of articles cited by EMDB/PDB/SASBDB data

  • Database of articles cited by EMDB, PDB, and SASBDB entries
  • Using PubMed data

Related info.:EMDB / PDB / SASBDB / Yorodumi / EMN Papers / Changes in new EM Navigator and Yorodumi

Read more