Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Recovery of particulate methane monooxygenase structure and activity in a lipid bilayer.
Journal, issue, pages	Science, Vol. 375, Issue 6586, Page 1287-1291, Year 2022
Publish date	Mar 18, 2022
Authors	Christopher W Koo / Frank J Tucci / Yuan He / Amy C Rosenzweig /
PubMed Abstract	Bacterial methane oxidation using the enzyme particulate methane monooxygenase (pMMO) contributes to the removal of environmental methane, a potent greenhouse gas. Crystal structures determined using ...Bacterial methane oxidation using the enzyme particulate methane monooxygenase (pMMO) contributes to the removal of environmental methane, a potent greenhouse gas. Crystal structures determined using inactive, detergent-solubilized pMMO lack several conserved regions neighboring the proposed active site. We show that reconstituting pMMO in nanodiscs with lipids extracted from the native organism restores methane oxidation activity. Multiple nanodisc-embedded pMMO structures determined by cryo-electron microscopy to 2.14- to 2.46-angstrom resolution reveal the structure of pMMO in a lipid environment. The resulting model includes stabilizing lipids, regions of the PmoA and PmoC subunits not observed in prior structures, and a previously undetected copper-binding site in the PmoC subunit with an adjacent hydrophobic cavity. These structures provide a revised framework for understanding and engineering pMMO function.
External links	Science / PubMed:35298269 / PubMed Central
Methods	EM (single particle)
Resolution	2.14 - 3.65 Å
Structure data	24826 7s4h EMDB-24826, PDB-7s4h: CryoEM structure of Methylococcus capsulatus (Bath) pMMO in a native lipid nanodisc at 2.14 Angstrom resolution Method: EM (single particle) / Resolution: 2.14 Å 24827 7s4i EMDB-24827, PDB-7s4i: CryoEM structure of Methylococcus capsulatus (Bath) pMMO in a native lipid nanodisc at 2.26 Angstrom resolution Method: EM (single particle) / Resolution: 2.26 Å 24828 7s4j EMDB-24828, PDB-7s4j: CryoEM structure of Methylococcus capsulatus (Bath) pMMO in a native lipid nanodisc at 2.16 Angstrom resolution Method: EM (single particle) / Resolution: 2.16 Å 24829 7s4k EMDB-24829, PDB-7s4k: CryoEM structure of Methylococcus capsulatus (Bath) pMMO in a native lipid nanodisc at 2.34 Angstrom resolution Method: EM (single particle) / Resolution: 2.36 Å 24830 7s4l EMDB-24830, PDB-7s4l: CryoEM structure of Methylotuvimicrobium alcaliphilum 20Z pMMO in a POPC nanodisc at 2.46 Angstrom resolution Method: EM (single particle) / Resolution: 2.46 Å 24831 7s4m EMDB-24831, PDB-7s4m: CryoEM structure of Methylocystis sp. str. Rockwell pMMO in a POPC nanodisc at 2.42 Angstrom resolution Method: EM (single particle) / Resolution: 2.42 Å 25683 7t4o EMDB-25683, PDB-7t4o: CryoEM structure of Methylococcus capsulatus (Bath) pMMO treated with potassium cyanide in a native lipid nanodisc at 3.65 Angstrom resolution Method: EM (single particle) / Resolution: 3.65 Å 25684 7t4p EMDB-25684: CryoEM structure of Methylococcus capsulatus (Bath) pMMO treated with potassium cyanide and copper in a native lipid nanodisc at 3.62 angstrom resolution PDB-7t4p: CryoEM structure of Methylococcus capsulatus (Bath) pMMO treated with potassium cyanide and copper in a native lipid nanodisc at 3.62 Angstrom resolution Method: EM (single particle) / Resolution: 3.62 Å
Chemicals	ChemComp-CU: COPPER (II) ION ChemComp-D10: DECANE ChemComp-PLC: DIUNDECYL PHOSPHATIDYL CHOLINE / phospholipidYM ChemComp-HXG: 1,2-dihexanoyl-sn-glycero-3-phosphocholine ChemComp-P1O: 1,2-DIDECANOYL-SN-GLYCERO-3-PHOSPHOCHOLINE / DDPC, phospholipidYM ChemComp-HOH: WATER ChemComp-6ER: (S)-2,3-bis(hexanoyloxy)propyl(2-(trimethylammonio)ethyl)phosphate ChemComp-D12: DODECANE
Source	Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) (bacteria) methylococcus capsulatus str. bath (bacteria) Methylotuvimicrobium alcaliphilum 20Z (bacteria) methylomicrobium alcaliphilum (strain dsm 19304 / ncimb 14124 / vkm b-2133 / 20z) (bacteria) methylocystis sp. atcc 49242 (bacteria)
Keywords	OXIDOREDUCTASE / Complex