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- EMDB-24831: CryoEM structure of Methylocystis sp. str. Rockwell pMMO in a POP... -
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Open data
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Basic information
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Title | CryoEM structure of Methylocystis sp. str. Rockwell pMMO in a POPC nanodisc at 2.42 Angstrom resolution | |||||||||
![]() | CryoEM structure of Methylocystis species strain Rockwell pMMO in a POPC nanodisc at 2.42 angstrom resolution | |||||||||
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Biological species | ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Koo CW / Rosenzweig AC | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Recovery of particulate methane monooxygenase structure and activity in a lipid bilayer. Authors: Christopher W Koo / Frank J Tucci / Yuan He / Amy C Rosenzweig / ![]() Abstract: Bacterial methane oxidation using the enzyme particulate methane monooxygenase (pMMO) contributes to the removal of environmental methane, a potent greenhouse gas. Crystal structures determined using ...Bacterial methane oxidation using the enzyme particulate methane monooxygenase (pMMO) contributes to the removal of environmental methane, a potent greenhouse gas. Crystal structures determined using inactive, detergent-solubilized pMMO lack several conserved regions neighboring the proposed active site. We show that reconstituting pMMO in nanodiscs with lipids extracted from the native organism restores methane oxidation activity. Multiple nanodisc-embedded pMMO structures determined by cryo-electron microscopy to 2.14- to 2.46-angstrom resolution reveal the structure of pMMO in a lipid environment. The resulting model includes stabilizing lipids, regions of the PmoA and PmoC subunits not observed in prior structures, and a previously undetected copper-binding site in the PmoC subunit with an adjacent hydrophobic cavity. These structures provide a revised framework for understanding and engineering pMMO function. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 201 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 16.9 KB 16.9 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 9.1 KB | Display | ![]() |
Images | ![]() | 116.4 KB | ||
Filedesc metadata | ![]() | 5.9 KB | ||
Others | ![]() | 23.2 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7s4mMC ![]() 7s4hC ![]() 7s4iC ![]() 7s4jC ![]() 7s4kC ![]() 7s4lC ![]() 7t4oC ![]() 7t4pC C: citing same article ( M: atomic model generated by this map |
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Links
EMDB pages | ![]() ![]() |
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Map
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Annotation | CryoEM structure of Methylocystis species strain Rockwell pMMO in a POPC nanodisc at 2.42 angstrom resolution | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.52 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: CryoEM structure of Methylocystis species strain Rockwell pMMO...
File | emd_24831_additional_1.map | ||||||||||||
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Annotation | CryoEM structure of Methylocystis species strain Rockwell pMMO in a POPC nanodisc at 2.42 angstrom resolution processed by deepEMhancer | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
+Entire : pMMO complex in a native lipid nanodisc
+Supramolecule #1: pMMO complex in a native lipid nanodisc
+Macromolecule #1: Particulate methane monooxygenase alpha subunit
+Macromolecule #2: Particulate methane monooxygenase beta subunit
+Macromolecule #3: Ammonia monooxygenase/methane monooxygenase, subunit C family protein
+Macromolecule #4: Unidentified Helix
+Macromolecule #5: COPPER (II) ION
+Macromolecule #6: 1,2-dihexanoyl-sn-glycero-3-phosphocholine
+Macromolecule #7: DECANE
+Macromolecule #8: DODECANE
+Macromolecule #9: water
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.3 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 1.02 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |