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TitleIn-situ cryo-ET of mouse embryos reveals cytoplasmic lattices contain ubiquitin-charged E2-E3 ligase assemblies.
Journal, issue, pagesEMBO J, Year 2026
Publish dateAug 10, 2026
AuthorsKashish Singh / Katarina Harasimov / Kathy K Niakan / Andrew P Carter /
PubMed AbstractCytoplasmic lattices (CPLs) are filamentous assemblies essential for mammalian embryonic development. They are known to regulate organelle organization, spindle assembly, and protein homeostasis, but ...Cytoplasmic lattices (CPLs) are filamentous assemblies essential for mammalian embryonic development. They are known to regulate organelle organization, spindle assembly, and protein homeostasis, but their molecular functions remain unclear. Here, we develop a strategy combining cryo-focused ion beam milling and cryo-electron tomography to resolve macromolecular complexes directly in mammalian embryos. Using this approach, we determine the in situ structure of cytoplasmic lattices within 6/8-cell mouse embryos at ~4.7 Å resolution. CPL filaments are built from multiple copies of at least fourteen proteins arranged into a ~4.5 MDa repeating unit. The repeat contains a central cavity that is open at the back and lined with multiple FBXW-SKP1 complexes and three modules, each containing the E2 ubiquitin-conjugating enzyme UBE2D and the E3 ligase UHRF1. We resolve two CPL states: one is consistent with a ubiquitin-charged UBE2D, where ubiquitin is held in an open, inactive conformation by binding the scaffold protein PADI6; the second lacks discernible ubiquitin density and shows structural changes compatible with ubiquitin becoming available for transfer. Our findings support a model in which CPLs function as large ubiquitin ligase assemblies during early embryonic development.
External linksEMBO J / PubMed:42575997
MethodsEM (subtomogram averaging)
Resolution4.7 - 10.0 Å
Structure data

55603

9t63

EMDB-55603, PDB-9t63:
Asymmetric unit of a cytoplasmic lattice filament repeat unit
Method: EM (subtomogram averaging) / Resolution: 4.7 Å

55604

9t64

EMDB-55604, PDB-9t64:
Cytoplasmic lattice filament repeat unit with the central FBXW-SKP1 complex
Method: EM (subtomogram averaging) / Resolution: 7.3 Å

55605

9t65

EMDB-55605, PDB-9t65:
Complex linking two cytoplasmic lattice filaments
Method: EM (subtomogram averaging) / Resolution: 5.6 Å

55606

9t66

EMDB-55606, PDB-9t66:
Complex linking two repeat units of a cytoplasmic lattice filament
Method: EM (subtomogram averaging) / Resolution: 5.3 Å

55607

9t67

EMDB-55607, PDB-9t67:
Cytoplasmic lattice filament repeat unit with ubiquitin
Method: EM (subtomogram averaging) / Resolution: 7.7 Å

EMDB-55608: Repeat unit of cytoplasmic lattices from intact mouse embryos
Method: EM (subtomogram averaging) / Resolution: 10.0 Å

55609

9t68

EMDB-55609, PDB-9t68:
Composite structure of the repeat unit of cytoplasmic lattice filament
Method: EM (subtomogram averaging) / Resolution: 8.0 Å

Source
  • mus musculus (house mouse)
KeywordsUNKNOWN FUNCTION / Ubiquitination / Complex / Filament

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