EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Choreography of rapid actin filament disassembly by coronin, cofilin, and AIP1.
ジャーナル・号・ページ	Cell, Vol. 188, Issue 24, Page 6845-6860.e27, Year 2025
掲載日	2025年11月26日
著者	Wout Oosterheert / Micaela Boiero Sanders / Oliver Hofnagel / Peter Bieling / Stefan Raunser /
PubMed 要旨	Rapid remodeling of actin filament (F-actin) networks is essential for the movement and morphogenesis of eukaryotic cells. The conserved actin-binding proteins coronin, cofilin, and actin-interacting ...Rapid remodeling of actin filament (F-actin) networks is essential for the movement and morphogenesis of eukaryotic cells. The conserved actin-binding proteins coronin, cofilin, and actin-interacting protein 1 (AIP1) act in synergy to promote rapid F-actin network disassembly, but the underlying mechanisms have remained elusive. Here, using cryo-electron microscopy (cryo-EM), we uncover the concerted molecular actions of coronin, cofilin, and AIP1 that lead to actin filament aging and severing. We find that the cooperative binding of coronin allosterically promotes inorganic phosphate release from F-actin and induces filament undertwisting, thereby priming the filament for cofilin binding. Cofilin then displaces coronin from the filament via a strand-restricted cooperative binding mechanism. The resulting cofilactin serves as a high-affinity platform for AIP1, which induces severing by acting as a clamp that disrupts inter-subunit filament contacts. In this "molecular squeezing" mechanism, AIP1 and not cofilin is responsible for filament severing. Our work redefines the role of key disassembly factors in actin dynamics.
リンク	Cell / PubMed:41075793
手法	EM (単粒子) / EM (らせん対称)
解像度	2.18 - 4.62 Å
構造データ	53093 9qew EMDB-53093, PDB-9qew: Cryo-EM structure of the undecorated actin filament in the ADP-Pi state. 手法: EM (単粒子) / 解像度: 2.18 Å 53094 9qey EMDB-53094, PDB-9qey: Cryo-EM structure of the actin filament bound by a single Coronin-1B molecule. 手法: EM (単粒子) / 解像度: 2.74 Å 53096 9qf2 EMDB-53096, PDB-9qf2: Cryo-EM structure of the fully Coronin-1B-decorated actin filament in the ADP state. 手法: EM (単粒子) / 解像度: 2.42 Å 53105 9qfb EMDB-53105, PDB-9qfb: Cryo-EM structure of the fully Coronin-1B-decorated actin filament in the ADP state. 手法: EM (らせん対称) / 解像度: 2.74 Å 53106 9qfd EMDB-53106, PDB-9qfd: Cryo-EM structure of the fully cofilin-1-decorated actin filament (cofilactin) 手法: EM (らせん対称) / 解像度: 2.61 Å 53107 9qfe EMDB-53107, PDB-9qfe: Cryo-EM structure of the actin filament hetero-decorated by Coronin-1 and Cofilin-1 on separate actin strands 手法: EM (単粒子) / 解像度: 3.12 Å 53108 9qfg EMDB-53108, PDB-9qfg: Cryo-EM structure of the actin filament hetero-decorated by Coronin-1 and Cofilin-1, strand boundary 手法: EM (単粒子) / 解像度: 3.49 Å 53110 9qfj EMDB-53110, PDB-9qfj: Cryo-EM structure of the cofilactin filament core at 2.3 Angstrom resolution. 手法: EM (単粒子) / 解像度: 2.31 Å EMDB-53111: Cryo-EM reconstruction of the Coronin-1B-decorated actin filament bound by two Cofilin-1 molecules (crosslinked) 手法: EM (単粒子) / 解像度: 4.43 Å EMDB-53112: Cryo-EM reconstruction of the Coronin-1B-decorated actin filament bound by three Cofilin-1 molecules (crosslinked) 手法: EM (単粒子) / 解像度: 4.36 Å EMDB-53113: Cryo-EM reconstruction of the Coronin-1B-decorated actin filament bound by four Cofilin-1 molecules (crosslinked) 手法: EM (単粒子) / 解像度: 4.33 Å 53114 9qfk EMDB-53114, PDB-9qfk: Cryo-EM structure of the Coronin-1B-decorated actin filament bound by one Cofilin-1 molecule (crosslinked) 手法: EM (単粒子) / 解像度: 3.99 Å EMDB-53118: Cryo-EM reconstruction of the cofilactin filament core bound by AIP1 手法: EM (単粒子) / 解像度: 4.62 Å 53119 9qfo EMDB-53119, PDB-9qfo: Cryo-EM structure of the cofilactin filament pointed end 手法: EM (単粒子) / 解像度: 2.96 Å 53121 9qfq EMDB-53121, PDB-9qfq: Cryo-EM structure of the cofilactin barbed end bound by AIP1 手法: EM (単粒子) / 解像度: 2.76 Å 53122 9qfw EMDB-53122, PDB-9qfw: Cryo-EM structure of the cofilactin barbed end bound by two AIP1 molecules 手法: EM (単粒子) / 解像度: 3.16 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-PO4: PHOSPHATE ION / ホスファ-ト ChemComp-HOH*: WATER
由来	homo sapiens (ヒト)
キーワード	STRUCTURAL PROTEIN / actin / filament / cytoskeleton / coronin / cofilin / pointed end / AIP1 / barbed end