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Structure paper

TitleStructures of human γδ T cell receptor-CD3 complex.
Journal, issue, pagesNature, Vol. 630, Issue 8015, Page 222-229, Year 2024
Publish dateApr 24, 2024
AuthorsWeizhi Xin / Bangdong Huang / Ximin Chi / Yuehua Liu / Mengjiao Xu / Yuanyuan Zhang / Xu Li / Qiang Su / Qiang Zhou /
PubMed AbstractGamma delta (γδ) T cells, a unique T cell subgroup, are crucial in various immune responses and immunopathology. The γδ T cell receptor (TCR), which is generated by γδ T cells, recognizes a ...Gamma delta (γδ) T cells, a unique T cell subgroup, are crucial in various immune responses and immunopathology. The γδ T cell receptor (TCR), which is generated by γδ T cells, recognizes a diverse range of antigens independently of the major histocompatibility complex. The γδ TCR associates with CD3 subunits, initiating T cell activation and holding great potential in immunotherapy. Here we report the structures of two prototypical human Vγ9Vδ2 and Vγ5Vδ1 TCR-CD3 complexes, revealing two distinct assembly mechanisms that depend on Vγ usage. The Vγ9Vδ2 TCR-CD3 complex is monomeric, with considerable conformational flexibility in the TCRγ-TCRδ extracellular domain and connecting peptides. The length of the connecting peptides regulates the ligand association and T cell activation. A cholesterol-like molecule wedges into the transmembrane region, exerting an inhibitory role in TCR signalling. The Vγ5Vδ1 TCR-CD3 complex displays a dimeric architecture, whereby two protomers nestle back to back through the Vγ5 domains of the TCR extracellular domains. Our biochemical and biophysical assays further corroborate the dimeric structure. Importantly, the dimeric form of the Vγ5Vδ1 TCR is essential for T cell activation. These findings reveal organizing principles of the γδ TCR-CD3 complex, providing insights into the unique properties of γδ TCR and facilitating immunotherapeutic interventions.
External linksNature / PubMed:38657677 / PubMed Central
MethodsEM (single particle)
Resolution3.0 - 9.5 Å
Structure data

36147

8jbv

EMDB-36147, PDB-8jbv:
Extracellular domain of gamma delta TCR
Method: EM (single particle) / Resolution: 3.02 Å

36149

8jc0

EMDB-36149, PDB-8jc0:
V gamma9 V delta2 TCR and CD3 complex in LMNG
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-36152: Vgamma5 Vdelta1 TCR complex (MPDI/TMDI)
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-36153: Vgamma5 Vdelta1 TCR complex (MPDII/TMDII)
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-36155: Vgamma5 Vdelta1 TCR complex (MPD/TMD)
Method: EM (single particle) / Resolution: 5.7 Å

36156

8jcb

EMDB-36156, PDB-8jcb:
Vgamma5 Vdelta1 T cell receptor complex
Method: EM (single particle) / Resolution: 9.5 Å

37904

8wxe

EMDB-37904, PDB-8wxe:
Vgamma5Vdelta1 EH TCR-CD3 complex
Method: EM (single particle) / Resolution: 4.0 Å

37914

8wy0

EMDB-37914, PDB-8wy0:
T cell receptor delta 2 gamma 9 with F283A, F290A, and F291A
Method: EM (single particle) / Resolution: 3.8 Å

37929

8wyi

EMDB-37929, PDB-8wyi:
T cell receptor delta 2 gamma 9 with TCRD TM domain chimera of TRAC
Method: EM (single particle) / Resolution: 3.9 Å

39128

8yc0

EMDB-39128, PDB-8yc0:
T cell receptor V delta2 V gamma9 in GDN
Method: EM (single particle) / Resolution: 4.12 Å

EMDB-39359: T cell receptor V delta2 V gamma9 in GDN (DeepEMhancer)
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-39361: Vgamma5 Vdelta1 TCR complex (MPDI/TMDI, DeepEMhancer)
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-39362: Vgamma5 Vdelta1 TCR complex (MPDII/TMDII, DeepEMhancer)
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-39363: V gamma9 V delta2 TCR and CD3 complex in LMNG (DeepEMhancer)
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-39367: Vgamma5 Vdelta1 TCR-CD3 complex (EH mutant, DeepEMhancer)
Method: EM (single particle) / Resolution: 4.0 Å

EMDB-39368: Extracellular domain of Vgamma5 Vdelta1 TCR (DeepEMhancer)
Method: EM (single particle) / Resolution: 3.0 Å

Chemicals

ChemComp-CLR:
CHOLESTEROL

Source
  • homo sapiens (human)
KeywordsIMMUNE SYSTEM / Receptor / immunity / immune

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