EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Roles of microtubules and LIS1 in dynein transport machinery assembly.
ジャーナル・号・ページ	Nature, Year 2026
掲載日	2026年2月18日
著者	Qinhui Rao / Jun Yang / Pengxin Chai / Steven Markus / Kai Zhang /
PubMed 要旨	Cytoplasmic dynein-1, a microtubule (MT)-based motor protein, requires dynactin and a coiled-coil adaptor to form the processive dynein-dynactin-adaptor (DDA) complex. The roles of MTs and dynein ...Cytoplasmic dynein-1, a microtubule (MT)-based motor protein, requires dynactin and a coiled-coil adaptor to form the processive dynein-dynactin-adaptor (DDA) complex. The roles of MTs and dynein regulator lissencephaly-1 (LIS1) in DDA assembly have remained elusive. Here we use cryo-electron microscopy to determine the structural basis of MT- and LIS1-mediated DDA assembly. We show that an adaptor-independent dynein-dynactin complex spontaneously forms on MTs with an intrinsic 2:1 stoichiometry in a highly efficient manner, driven by parallel alignment of dynein tails upon MT binding. Adaptors can wedge into and exchange within the assembled MT-bound dynein-dynactin complex; these processes are enabled by relative rotations between dynein and dynactin and facilitated by the dynein light-intermediate chains that assist the adaptor 'search' mechanism. Although LIS1 is dispensable for efficient DD(A)-MT assembly, its presence expands the conformational landscape of DD(A) assemblies on MTs. Cryo-electron microscopy reveals that LIS1 bridges dynactin p150 and dynein in both the closed Phi-like and open prepowerstroke states, stabilizing low-MT-affinity intermediates that tether dynein molecules in proximity to MTs and prime them for subsequent DD(A) assembly through alternative pathways. These findings demonstrate the dynamic adaptability of the dynein transport machinery and the coordinated roles of MTs and LIS1 in DDA assembly.
リンク	Nature / PubMed:41708859
手法	EM (単粒子)
解像度	3.5 - 15.0 Å
構造データ	46843 9dgp EMDB-46843, PDB-9dgp: Motor domain of dynein-1 complex on microtubules 手法: EM (単粒子) / 解像度: 3.5 Å 46844 9dgq EMDB-46844, PDB-9dgq: Structure of dynein-1 on microtubules 手法: EM (単粒子) / 解像度: 11.0 Å 46845 9dgr EMDB-46845, PDB-9dgr: Composite structure of dynein-dynactin on microtubules 手法: EM (単粒子) / 解像度: 15.0 Å 46846 9dgs EMDB-46846, PDB-9dgs: Dynactin and dynein tail region of dynein-dynactin complex on microtubules 手法: EM (単粒子) / 解像度: 3.9 Å 46847 9dgt EMDB-46847, PDB-9dgt: structure of dynactin, dynein tail with one BICDR from dynein-dynactin-BICDR on microtubules 手法: EM (単粒子) / 解像度: 7.2 Å 46848 9dgu EMDB-46848, PDB-9dgu: structure of dynactin, dynein tail with two BICDR from dynein-dynactin-BICDR on microtubules 手法: EM (単粒子) / 解像度: 7.1 Å 46849 9dgv EMDB-46849, PDB-9dgv: structure of dynactin, dynein tail with TRAK2 from dynein-dynactin-TRAK2 on microtubules 手法: EM (単粒子) / 解像度: 8.8 Å EMDB-47061: Consensus map of dynein-dynactin on microtubules 手法: EM (単粒子) / 解像度: 12.0 Å 73173 9ync EMDB-73173, PDB-9ync: Motor domains of phi-like human dynein-1 bound to dynactin-p150glued and LIS1 手法: EM (単粒子) / 解像度: 3.93 Å 73174 9ynd EMDB-73174, PDB-9ynd: Motor domain of human dynein-1 in pre-power stroke bound to dynactin-p150glued-CC1B and LIS1 手法: EM (単粒子) / 解像度: 4.26 Å 73175 9yne EMDB-73175, PDB-9yne: Motor domain of human dynein-1 in pre-power stroke bound to dynactin-p150glued-CC1B-ICD and LIS1 手法: EM (単粒子) / 解像度: 8.46 Å 73176 9ynf EMDB-73176, PDB-9ynf: Motor domain of human dynein-1 in post1 state 手法: EM (単粒子) / 解像度: 3.92 Å 73178 9yng EMDB-73178, PDB-9yng: Dynactin and dynein-1 tail region of dynein-dynactin complex on microtubule in the presence of LIS1 手法: EM (単粒子) / 解像度: 4.07 Å 73179 9ynh EMDB-73179, PDB-9ynh: Full-length human cytoplasmic dynein-1 in phi-like state bound to dynactin-p150glued and LIS1 手法: EM (単粒子) / 解像度: 5.5 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, エネルギー貯蔵分子YM ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, エネルギー貯蔵分子YM ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, エネルギー貯蔵分子類似体YM ChemComp-MG: Unknown entry ChemComp-ZN*: Unknown entry
由来	sus scrofa (ブタ) mus musculus (ハツカネズミ) homo sapiens (ヒト)
キーワード	MOTOR PROTEIN / dynein / microtubule / dynactin / BICDR / TRAK2 / Dynein-1 / phi-like conformation / Lis1 / p150glued / p150