EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cooperativity in E. coli aspartate transcarbamoylase is tuned by allosteric breathing.
ジャーナル・号・ページ	Nat Commun, Year 2026
掲載日	2026年3月20日
著者	Robert C Miller / Michael G Patterson / Neti Bhatt / Xiaokun Pei / Nozomi Ando /
PubMed 要旨	Aspartate transcarbamoylase (ATCase) from Escherichia coli catalyzes a key step in pyrimidine nucleotide biosynthesis and has long served as a model for allosteric regulation. Despite decades of ...Aspartate transcarbamoylase (ATCase) from Escherichia coli catalyzes a key step in pyrimidine nucleotide biosynthesis and has long served as a model for allosteric regulation. Despite decades of study, how nucleotide binding at distant regulatory sites controls cooperativity between active sites remained unresolved. Here we show that ATCase does not simply interconvert between two conformations, as traditionally depicted, but instead samples a continuum of conformations that tune enzyme cooperativity. Using complementary cryo-electron microscopy, small-angle X-ray scattering, and crystallography under conditions that ensure full assembly of the allosteric sites, we show that ATCase behaves like a flexible balloon whose global "breathing" motions directly regulate activity: compression enforces high cooperativity, inhibiting the enzyme, whereas expansion relieves this cooperativity and activates the enzyme. We further show that all four ribonucleoside triphosphates act in symmetric pairs to tune this motion, with the pyrimidines CTP and UTP compressing the enzyme to limit further pyrimidine production, and the purines ATP and GTP expanding it to balance pyrimidine and purine pools. Together, these findings uncover a dynamic breathing mechanism for long-range allosteric communication in ATCase.
リンク	Nat Commun / PubMed:41862478
手法	EM (単粒子) / X線回折
解像度	2.19 - 3.85 Å
構造データ	47956 9eek EMDB-47956, PDB-9eek: Cryo-EM model of E. coli aspartate transcarbamoylase in the ligand-free T-state 手法: EM (単粒子) / 解像度: 2.62 Å 47957 9eel EMDB-47957, PDB-9eel: Cryo-EM model of E. coli aspartate transcarbamoylase in the T-state complexed with CP, CTP, UTP, and Mg2+ 手法: EM (単粒子) / 解像度: 3.45 Å 47958 9eem EMDB-47958, PDB-9eem: Cryo-EM model of E. coli aspartate transcarbamoylase in the R-state complexed with CP, succinate, CTP, UTP, and Mg2+ 手法: EM (単粒子) / 解像度: 3.05 Å 47959 9een EMDB-47959, PDB-9een: Cryo-EM model of E. coli aspartate transcarbamoylase in the T-state complexed with CP, CTP, and Mg2+ 手法: EM (単粒子) / 解像度: 3.85 Å 47960 9eeo EMDB-47960, PDB-9eeo: Cryo-EM model of E. coli aspartate transcarbamoylase in the R-state complexed with CP, succinate, CTP, and Mg2+ 手法: EM (単粒子) / 解像度: 3.53 Å 47961 9eep EMDB-47961, PDB-9eep: Cryo-EM model of E. coli aspartate transcarbamoylase in the R-state complexed with CP and succinate 手法: EM (単粒子) / 解像度: 3.08 Å 47963 9eeq EMDB-47963, PDB-9eeq: Cryo-EM model of E. coli aspartate transcarbamoylase in the R-state complexed with CP, succinate, ATP, and Mg2+ 手法: EM (単粒子) / 解像度: 3.08 Å 47964 9eer EMDB-47964, PDB-9eer: Cryo-EM model of E. coli aspartate transcarbamoylase in the R-state complexed with CP, succinate, ATP, GTP, and Mg2+ 手法: EM (単粒子) / 解像度: 3.26 Å 47965 9ees EMDB-47965, PDB-9ees: Cryo-EM model of E. coli aspartate transcarbamoylase in an expanded state complexed with CP, ATP, GTP, and Mg2+ 手法: EM (単粒子) / 解像度: 3.27 Å 47966 9eeu EMDB-47966, PDB-9eeu: Cryo-EM model of E. coli aspartate transcarbamoylase in the T-state complexed with CP, ATP, and Mg2+ 手法: EM (単粒子) / 解像度: 3.27 Å PDB-9eeh: Crystal structure of E. coli aspartate transcarbamoylase in the R-state complexed with PALA, ATP, GTP, and Mg2+ 手法: X-RAY DIFFRACTION / 解像度: 2.19 Å PDB-9eej: Crystal structure of E. coli aspartate transcarbamoylase in the R-state complexed with CP, succinate, ATP, and Mg2+ 手法: X-RAY DIFFRACTION / 解像度: 3 Å
化合物	ChemComp-PAL: N-(PHOSPHONACETYL)-L-ASPARTIC ACID ChemComp-ZN: Unknown entry ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP, エネルギー貯蔵分子YM ChemComp-HOH: WATER ChemComp-CP: PHOSPHORIC ACID MONO(FORMAMIDE)ESTER ChemComp-SIN: SUCCINIC ACID ChemComp-CTP: CYTIDINE-5'-TRIPHOSPHATE ChemComp-UTP: URIDINE 5'-TRIPHOSPHATE / UTP*YM
由来	escherichia coli (大腸菌)
キーワード	CYTOSOLIC PROTEIN / Complex / Allostery / ATCase / R-state / ATP / GTP / PALA / CP / succinate / T-state / CTP / UTP / MWC / Expanded