EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural diversity of axonemes across mammalian motile cilia.
ジャーナル・号・ページ	Nature, Vol. 637, Issue 8048, Page 1170-1177, Year 2025
掲載日	2025年1月1日
著者	Miguel Ricardo Leung / Chen Sun / Jianwei Zeng / Jacob R Anderson / Qingwei Niu / Wei Huang / Willem E M Noteborn / Alan Brown / Tzviya Zeev-Ben-Mordehai / Rui Zhang /
PubMed 要旨	Reproduction, development and homeostasis depend on motile cilia, whose rhythmic beating is powered by a microtubule-based molecular machine called the axoneme. Although an atomic model of the ...Reproduction, development and homeostasis depend on motile cilia, whose rhythmic beating is powered by a microtubule-based molecular machine called the axoneme. Although an atomic model of the axoneme is available for the alga Chlamydomonas reinhardtii, structures of mammalian axonemes are incomplete. Furthermore, we do not fully understand how molecular structures of axonemes vary across motile-ciliated cell types in the body. Here we use cryoelectron microscopy, cryoelectron tomography and proteomics to resolve the 96-nm modular repeat of axonemal doublet microtubules (DMTs) from both sperm flagella and epithelial cilia of the oviduct, brain ventricles and respiratory tract. We find that sperm DMTs are the most specialized, with epithelial cilia having only minor differences across tissues. We build a model of the mammalian sperm DMT, defining the positions and interactions of 181 proteins including 34 newly identified proteins. We elucidate the composition of radial spoke 3 and uncover binding sites of kinases associated with regeneration of ATP and regulation of ciliary motility. We discover a sperm-specific, axoneme-tethered T-complex protein ring complex (TRiC) chaperone that may contribute to construction or maintenance of the long flagella of mammalian sperm. We resolve axonemal dyneins in their prestroke states, illuminating conformational changes that occur during ciliary movement. Our results illustrate how elements of chemical and mechanical regulation are embedded within the axoneme, providing valuable resources for understanding the aetiology of ciliopathy and infertility, and exemplifying the discovery power of modern structural biology.
リンク	Nature / PubMed:39743588 / PubMed Central
手法	EM (サブトモグラム平均) / EM (単粒子)
解像度	3.4 - 12.96 Å
構造データ	EMDB-45677: 96-nm repeat of axonemal doublet microtubules from porcine oviduct cilia, subtomogram average segment 1 of 4 手法: EM (サブトモグラム平均) / 解像度: 9.6 Å EMDB-45678: 96-nm repeat of axonemal doublet microtubules from porcine oviduct cilia, subtomogram average segment 2 of 4 手法: EM (サブトモグラム平均) / 解像度: 9.4 Å EMDB-45679: 96-nm repeat of axonemal doublet microtubules from porcine oviduct cilia, subtomogram average segment 3 of 4 手法: EM (サブトモグラム平均) / 解像度: 9.3 Å EMDB-45680: 96-nm repeat of axonemal doublet microtubules from porcine oviduct cilia, subtomogram average segment 4 of 4 手法: EM (サブトモグラム平均) / 解像度: 10.0 Å EMDB-45683: Dynein motor inner dynein arm subform c (IDAc) from bovine Fallopian tube 手法: EM (単粒子) / 解像度: 7.92 Å EMDB-45684: Dynein motor inner dynein arm subform e (IDAe) from bovine Fallopian tube 手法: EM (単粒子) / 解像度: 8.82 Å EMDB-45685: Dynein motor inner dynein arm subform f (IDAf) from bovine Fallopian tube 手法: EM (単粒子) / 解像度: 8.79 Å EMDB-45686: The nexin-dynein regulatory complex (N-DRC) from bovine Fallopian tube, distal part 手法: EM (単粒子) / 解像度: 9.15 Å EMDB-45687: The nexin-dynein regulatory complex (N-DRC) from bovine Fallopian tube, base part 手法: EM (単粒子) / 解像度: 3.83 Å EMDB-45688: The Outer Dynein Arm (ODA) complex from bovine Fallopian tube 手法: EM (単粒子) / 解像度: 8.68 Å EMDB-45689: The Outer Dynein Arm-Docking Complex (ODA-DC) from bovine Fallopian tube 手法: EM (単粒子) / 解像度: 3.64 Å EMDB-45690: Dyneins in pre-state from bovine Fallopian tube (part 1) 手法: EM (単粒子) / 解像度: 7.8 Å EMDB-45691: Dyneins in pre-state from bovine Fallopian tube (part 2) 手法: EM (単粒子) / 解像度: 7.57 Å EMDB-45692: Radial Spoke 1 from bovine Fallopian tube, base and stalk 手法: EM (単粒子) / 解像度: 6.59 Å EMDB-45693: Radial Spoke 1 from bovine Fallopian tube, stalk and head 手法: EM (単粒子) / 解像度: 8.67 Å EMDB-45694: Radial Spoke 2 from bovine Fallopian tube, base and stalk 手法: EM (単粒子) / 解像度: 8.06 Å EMDB-45695: Radial Spoke 2 from bovine Fallopian tube, stalk and head 手法: EM (単粒子) / 解像度: 9.21 Å EMDB-45696: Radial Spoke 3 from bovine Fallopian tube, base and stalk 手法: EM (単粒子) / 解像度: 5.86 Å EMDB-45697: Radial Spoke 3 from bovine Fallopian tube, stalk and head 手法: EM (単粒子) / 解像度: 9.95 Å EMDB-45698: FAP206 from bovine Fallopian tube 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-45699: Dynein motor inner dynein arm subform c (IDAc) from Porcine brain ventricles 手法: EM (単粒子) / 解像度: 7.8 Å EMDB-45713: Radial Spoke 3 from Pocine brain ventricles, stalk and head 手法: EM (単粒子) / 解像度: 9.72 Å EMDB-45714: The nexin-dynein regulatory complex (N-DRC) from human fallopian tube, distal part 手法: EM (単粒子) / 解像度: 6.77 Å EMDB-45715: The nexin-dynein regulatory complex (N-DRC) from human fallopian tube, base part 手法: EM (単粒子) / 解像度: 3.95 Å EMDB-45716: The Outer Dynein Arm (ODA) complex from human fallopian tube 手法: EM (単粒子) / 解像度: 7.8 Å EMDB-45717: The Outer Dynein Arm-Docking Complex (ODA-DC) from human fallopian tube 手法: EM (単粒子) / 解像度: 3.47 Å EMDB-45718: Dyneins in pre-state from human fallopian tube (part 1) 手法: EM (単粒子) / 解像度: 6.89 Å EMDB-45719: Dyneins in pre-state from human fallopian tube (part 2) 手法: EM (単粒子) / 解像度: 7.79 Å EMDB-45720: Radial Spoke 1 from human fallopian tube, base and stalk 手法: EM (単粒子) / 解像度: 6.74 Å EMDB-45721: Radial Spoke 1 from human fallopian tube, stalk and head 手法: EM (単粒子) / 解像度: 10.55 Å EMDB-45722: Radial Spoke 2 from human fallopian tube, base and stalk 手法: EM (単粒子) / 解像度: 7.54 Å EMDB-45723: Radial Spoke 2 from human fallopian tube, stalk and head 手法: EM (単粒子) / 解像度: 12.96 Å EMDB-45724: Radial Spoke 3 from human fallopian tube, base and stalk 手法: EM (単粒子) / 解像度: 6.52 Å EMDB-45725: Radial Spoke 3 from human fallopian tube, stalk and head 手法: EM (単粒子) / 解像度: 5.83 Å EMDB-45783: Composite map of bovine Fallopian tube cilia in 96nm repeat 手法: EM (単粒子) / 解像度: 3.52 Å EMDB-45784: Composite map of porcine brain ventricle cilia in 96nm repeat 手法: EM (単粒子) / 解像度: 3.65 Å EMDB-45785: Composite map of human Fallopian tube cilia in 96nm repeat 手法: EM (単粒子) / 解像度: 3.56 Å EMDB-45790: Dynein motor inner dynein arm subform f (IDAf) from human fallopian tube 手法: EM (単粒子) / 解像度: 9.26 Å 45801 9cpb EMDB-45801: Cryo-EM structure of bovine Fallopian tube cilia doublet microtubule (48-nm periodicity) PDB-9cpb: Atomic model of bovine Fallopian tube cilia doublet microtubule (48-nm periodicity) 手法: EM (単粒子) / 解像度: 3.52 Å 45802 9cpc EMDB-45802: Cryo-EM structure of porcine brain ventricles cilia doublet microtubule (48-nm periodicity) PDB-9cpc: Atomic model of porcine brain ventricles cilia doublet microtubule (48-nm periodicity) 手法: EM (単粒子) / 解像度: 3.65 Å EMDB-50866: 96-nm repeat of axonemal doublet microtubules from bovine sperm, RS1 base local refinement 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-50867: 96-nm repeat of axonemal doublet microtubules from bovine sperm, RS1 stalk local refinement 手法: EM (単粒子) / 解像度: 5.1 Å EMDB-50868: 96-nm repeat of axonemal doublet microtubules from bovine sperm, RS1 head local refinement 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-50869: 96-nm repeat of axonemal doublet microtubules from bovine sperm, RS2 base local refinement 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-50870: 96-nm repeat of axonemal doublet microtubules from bovine sperm, RS2 stalk local refinement 手法: EM (単粒子) / 解像度: 4.5 Å EMDB-50871: 96-nm repeat of axonemal doublet microtubules from bovine sperm, RS2 head local refinement 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-50872: 96-nm repeat of axonemal doublet microtubules from bovine sperm, RS3 base local refinement 手法: EM (単粒子) / 解像度: 4.3 Å EMDB-50873: 96-nm repeat of axonemal doublet microtubules from bovine sperm, RS3 stalk local refinement 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-50874: 96-nm repeat of axonemal doublet microtubules from bovine sperm, RS3 head local refinement 手法: EM (単粒子) / 解像度: 4.6 Å EMDB-50875: 96-nm repeat of axonemal doublet microtubules from bovine sperm, RS1 barrel local refinement 手法: EM (単粒子) / 解像度: 7.4 Å EMDB-50876: 96-nm repeat of axonemal doublet microtubules from bovine sperm, RS2-RS3 bridge local refinement 手法: EM (単粒子) / 解像度: 7.0 Å EMDB-50877: 96-nm repeat of axonemal doublet microtubules from bovine sperm, N-DRC baseplate local refinement 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-50878: 96-nm repeat of axonemal doublet microtubules from bovine sperm, N-DRC distal lobe local refinement 手法: EM (単粒子) / 解像度: 4.7 Å EMDB-50879: 96-nm repeat of axonemal doublet microtubules from bovine sperm, IDAab local refinement 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-50880: 96-nm repeat of axonemal doublet microtubules from bovine sperm, IDAc local refinement 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-50881: 96-nm repeat of axonemal doublet microtubules from bovine sperm, IDAe local refinement 手法: EM (単粒子) / 解像度: 7.2 Å EMDB-50882: 96-nm repeat of axonemal doublet microtubules from bovine sperm, IDAf IC/LC local refinement 手法: EM (単粒子) / 解像度: 8.2 Å EMDB-50883: 96-nm repeat of axonemal doublet microtubules from bovine sperm, IDAf motors pre-state local refinement part 1 手法: EM (単粒子) / 解像度: 4.6 Å EMDB-50884: 96-nm repeat of axonemal doublet microtubules from bovine sperm, IDAf motors pre-state local refinement part 2 手法: EM (単粒子) / 解像度: 5.9 Å EMDB-50885: 96-nm repeat of axonemal doublet microtubules from bovine sperm, ODA-DC local refinement 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-50886: 96-nm repeat of axonemal doublet microtubules from bovine sperm, ODA local refinement 手法: EM (単粒子) / 解像度: 7.1 Å EMDB-50887: 96-nm repeat of axonemal doublet microtubules from bovine sperm, ARMH1 subcomplex local refinement 手法: EM (単粒子) / 解像度: 4.6 Å EMDB-53135: native axonemal doublet microtubule from bovine sperm, 96-nm repeat (consensus refinement, microtubule-centered) 手法: EM (単粒子) / 解像度: 3.5 Å PDB-9fqr: 96-nm repeat of axonemal doublet microtubules from bovine sperm 手法: ELECTRON MICROSCOPY / 解像度: 5 Å
化合物	ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM
由来	sus scrofa (ブタ) bos taurus (ウシ) Homo sapiens (ヒト)
キーワード	STRUCTURAL PROTEIN / axoneme / cilia / microtubule / dynein / Fallopian tube / doublet microtubule (DMT) / brain ventricle / sperm