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- PDB-9cpc: Atomic model of porcine brain ventricles cilia doublet microtubul... -

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Basic information

Entry
Database: PDB / ID: 9cpc
TitleAtomic model of porcine brain ventricles cilia doublet microtubule (48-nm periodicity)
Components
  • (Chromosome 1 ...) x 2
  • (Cilia and flagella associated protein ...) x 6
  • (Cilia- and flagella-associated protein ...) x 4
  • (Ciliary microtubule inner protein ...) x 2
  • (EF-hand domain ...) x 2
  • (Outer dynein ...) x 2
  • (Tektin) x 4
  • Armadillo repeat-containing protein 4 isoform 1
  • CFAP107/C1orf158
  • Cilia-and flagella-associated protein 96
  • Coiled-coil domain-containing protein 114 isoform X2
  • EF-hand calcium binding domain 1
  • EF-hand domain-containing family member C2
  • EFCAB6
  • Enkurin
  • Meiosis-specific nuclear structural protein 1
  • Nucleoside diphosphate kinase homolog 7
  • Parkin coregulated
  • Piercer of microtubule wall 1
  • Protein Flattop
  • RIB43A domain with coiled-coils 2
  • RIB43A-like with coiled-coils protein 1
  • Sperm acrosome associated 9
  • Sperm-associated antigen 8 isoform 1
  • Tubulin alpha chain
  • Tubulin beta chain
KeywordsSTRUCTURAL PROTEIN / axoneme / cilia / microtubule / dynein / brain ventricle / doublet microtubule (DMT)
Function / homology
Function and homology information


regulation of cilium beat frequency / outer dynein arm docking complex / determination of heart left/right asymmetry / mucociliary clearance / outer acrosomal membrane / epithelial cilium movement involved in determination of left/right asymmetry / regulation of brood size / establishment of left/right asymmetry / 9+0 motile cilium / protein localization to motile cilium ...regulation of cilium beat frequency / outer dynein arm docking complex / determination of heart left/right asymmetry / mucociliary clearance / outer acrosomal membrane / epithelial cilium movement involved in determination of left/right asymmetry / regulation of brood size / establishment of left/right asymmetry / 9+0 motile cilium / protein localization to motile cilium / axonemal B tubule inner sheath / axonemal A tubule inner sheath / sperm axoneme assembly / inner dynein arm assembly / positive regulation of feeding behavior / regulation of calcineurin-NFAT signaling cascade / regulation of microtubule nucleation / sperm principal piece / outer dynein arm assembly / 9+0 non-motile cilium / microtubule sliding / cerebrospinal fluid circulation / regulation of cilium beat frequency involved in ciliary motility / epithelial cilium movement involved in extracellular fluid movement / regulation of store-operated calcium entry / 9+2 motile cilium / intraciliary transport / regulation of cilium assembly / cilium movement / ciliary transition zone / axoneme assembly / left/right axis specification / calcium ion sensor activity / axonemal microtubule / gamma-tubulin ring complex / ventricular system development / manchette / flagellated sperm motility / positive regulation of cilium assembly / UTP biosynthetic process / CTP biosynthetic process / determination of left/right symmetry / intermediate filament / motile cilium / nucleoside diphosphate kinase activity / ciliary base / GTP biosynthetic process / microtubule organizing center / positive regulation of cell motility / regulation of neuron projection development / receptor clustering / cytoplasmic microtubule / beta-tubulin binding / cerebral cortex cell migration / regulation of cell division / axoneme / mitotic cytokinesis / spermatid development / centriolar satellite / cilium assembly / single fertilization / alpha-tubulin binding / sperm flagellum / microtubule-based process / sperm midpiece / 3'-5' exonuclease activity / Hsp70 protein binding / centriole / cellular response to leukemia inhibitory factor / mitotic spindle organization / acrosomal vesicle / ciliary basal body / guanyl-nucleotide exchange factor activity / meiotic cell cycle / lung development / Hsp90 protein binding / G protein-coupled receptor binding / brain development / multicellular organism growth / cilium / structural constituent of cytoskeleton / mitotic spindle / SH3 domain binding / microtubule cytoskeleton organization / spindle pole / microtubule cytoskeleton / nuclear envelope / protein localization / mitotic cell cycle / actin binding / heart development / cell body / microtubule binding / gene expression / spermatogenesis / vesicle / microtubule / Ras protein signal transduction / cytoskeleton / calmodulin binding
Similarity search - Function
Outer dynein arm-docking complex subunit 4 / Protein of unknown function DUF4586 / Outer dynein arm-docking complex subunit 3 / Cilia-and flagella-associated protein 96 / FAM183 family / FAM183A and FAM183B related / Sperm-associated antigen 8 / Sperm acrosome-associated protein 9 / Cilia- and flagella-associated protein 95 / NDPK7, first NDPk domain ...Outer dynein arm-docking complex subunit 4 / Protein of unknown function DUF4586 / Outer dynein arm-docking complex subunit 3 / Cilia-and flagella-associated protein 96 / FAM183 family / FAM183A and FAM183B related / Sperm-associated antigen 8 / Sperm acrosome-associated protein 9 / Cilia- and flagella-associated protein 95 / NDPK7, first NDPk domain / Cilia- and flagella-associated protein 107 / : / : / : / Sperm acrosome-associated protein 9 / Protein CFAP95 / ODAD1 central coiled coil region / Cilia- and flagella-associated protein 143 / Cilia and flagella associated protein 107 / Tektin / Cilia- and flagella-associated protein 276 / : / Tektin family / Protein of unknown function (DUF3695) / Cilia- and flagella-associated protein 77 / Cilia- and flagella-associated protein 77 / Uncharacterised protein FAM166/UPF0605 / Protein Flattop / Ciliary microtubule inner protein 2B-like / Flattop / Nucleoside diphosphate kinase 7 / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 141 / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 141 / Meiosis-specific nuclear structural protein 1 / Cilia- and flagella-associated protein 45 / NDPK7, second NDPk domain / Cilia- and flagella- associated protein 210 / CFAP53/TCHP / Trichohyalin-plectin-homology domain / Trichohyalin-plectin-homology domain / RIB43A / RIB43A / DM10 domain / EF-hand domain-containing protein EFHC1/EFHC2/EFHB / DM10 domain / DM10 domain profile. / Domains in hypothetical proteins in Drosophila, C. elegans and mammals. Occurs singly in some nucleoside diphosphate kinases. / Enkurin domain / : / Calmodulin-binding / Enkurin domain profile. / : / CFA20 domain / Cilia- and flagella-associated protein 20/CFAP20DC / CFA20 domain / Parkin co-regulated protein / Recoverin family / Parkin co-regulated protein / EF hand / Nucleoside diphosphate kinase / Nucleoside diphosphate kinase-like domain / Nucleoside diphosphate kinase / NDK / Nucleoside diphosphate kinase-like domain superfamily / HEAT repeats / Armadillo/plakoglobin ARM repeat profile. / Armadillo/beta-catenin-like repeat / Armadillo/beta-catenin-like repeats / Armadillo / Tetratricopeptide repeat / Alpha tubulin / Tubulin-beta mRNA autoregulation signal. / Beta tubulin, autoregulation binding site / Beta tubulin / TPR repeat profile. / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tetratricopeptide repeats / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily / Tetratricopeptide repeat / EF-hand domain pair / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / Armadillo-like helical / EF-hand domain pair
Similarity search - Domain/homology
GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / Cilia- and flagella-associated protein 53 / EF-hand domain containing 1 / Cilia- and flagella-associated protein 45 / Armadillo repeat-containing protein 4 isoform 1 / Sperm-associated antigen 8 isoform 1 / Tektin / Tektin / Chromosome 1 C15orf65 homolog ...GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / Cilia- and flagella-associated protein 53 / EF-hand domain containing 1 / Cilia- and flagella-associated protein 45 / Armadillo repeat-containing protein 4 isoform 1 / Sperm-associated antigen 8 isoform 1 / Tektin / Tektin / Chromosome 1 C15orf65 homolog / Meiosis-specific nuclear structural protein 1 / Enkurin / EF-hand calcium binding domain 1 / Tektin / Cilia-and flagella-associated protein 96 / Tubulin beta chain / Tubulin alpha chain / Outer dynein arm docking complex subunit 3 / Cilia and flagella associated protein 161 / Parkin coregulated / Cilia and flagella associated protein 276 / Chromosome 1 C9orf135 homolog / Nucleoside diphosphate kinase homolog 7 / Cilia and flagella associated protein 141 / RIB43A-like with coiled-coils protein 1 / Sperm acrosome associated 9 / Tektin / Ciliary microtubule inner protein 2B / Protein Flattop / Uncharacterized protein / Ciliary microtubule inner protein 2B / Outer dynein arm-docking complex subunit 4 / Uncharacterized protein / EF-hand domain family member B / Cilia and flagella associated protein 77 / Cilia and flagella associated protein 210 / Cilia and flagella associated protein 144 / RIB43A domain with coiled-coils 2 / Cilia- and flagella-associated protein 52 / Cilia-and flagella-associated protein 20 / Coiled-coil domain-containing protein 114 isoform X2 / EF-hand domain-containing family member C2
Similarity search - Component
Biological speciesSus scrofa (pig)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.65 Å
AuthorsSun, C. / Zeng, J. / Zhang, R.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)1R01 GM138854 United States
CitationJournal: Nature / Year: 2025
Title: Structural diversity of axonemes across mammalian motile cilia.
Authors: Miguel Ricardo Leung / Chen Sun / Jianwei Zeng / Jacob R Anderson / Qingwei Niu / Wei Huang / Willem E M Noteborn / Alan Brown / Tzviya Zeev-Ben-Mordehai / Rui Zhang /
Abstract: Reproduction, development and homeostasis depend on motile cilia, whose rhythmic beating is powered by a microtubule-based molecular machine called the axoneme. Although an atomic model of the ...Reproduction, development and homeostasis depend on motile cilia, whose rhythmic beating is powered by a microtubule-based molecular machine called the axoneme. Although an atomic model of the axoneme is available for the alga Chlamydomonas reinhardtii, structures of mammalian axonemes are incomplete. Furthermore, we do not fully understand how molecular structures of axonemes vary across motile-ciliated cell types in the body. Here we use cryoelectron microscopy, cryoelectron tomography and proteomics to resolve the 96-nm modular repeat of axonemal doublet microtubules (DMTs) from both sperm flagella and epithelial cilia of the oviduct, brain ventricles and respiratory tract. We find that sperm DMTs are the most specialized, with epithelial cilia having only minor differences across tissues. We build a model of the mammalian sperm DMT, defining the positions and interactions of 181 proteins including 34 newly identified proteins. We elucidate the composition of radial spoke 3 and uncover binding sites of kinases associated with regeneration of ATP and regulation of ciliary motility. We discover a sperm-specific, axoneme-tethered T-complex protein ring complex (TRiC) chaperone that may contribute to construction or maintenance of the long flagella of mammalian sperm. We resolve axonemal dyneins in their prestroke states, illuminating conformational changes that occur during ciliary movement. Our results illustrate how elements of chemical and mechanical regulation are embedded within the axoneme, providing valuable resources for understanding the aetiology of ciliopathy and infertility, and exemplifying the discovery power of modern structural biology.
History
DepositionJul 18, 2024Deposition site: RCSB / Processing site: RCSB
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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
1A: Armadillo repeat-containing protein 4 isoform 1
1B: Armadillo repeat-containing protein 4 isoform 1
1C: Outer dynein arm-docking complex subunit 4
1D: Outer dynein arm-docking complex subunit 4
1F: EF-hand calcium binding domain 1
1G: EF-hand calcium binding domain 1
1H: Coiled-coil domain-containing protein 114 isoform X2
1I: Coiled-coil domain-containing protein 114 isoform X2
1J: Coiled-coil domain-containing protein 114 isoform X2
1L: Outer dynein arm docking complex subunit 3
1M: Outer dynein arm docking complex subunit 3
1N: Outer dynein arm docking complex subunit 3
1P: EFCAB6
1Q: EFCAB6
1S: Cilia- and flagella-associated protein 52
1T: Cilia- and flagella-associated protein 52
1U: Cilia- and flagella-associated protein 52
1W: Cilia- and flagella-associated protein 45
1X: Cilia- and flagella-associated protein 45
1Y: Cilia- and flagella-associated protein 45
1Z: Cilia- and flagella-associated protein 45
2B: Cilia and flagella associated protein 210
2C: Cilia and flagella associated protein 210
2E: Cilia and flagella associated protein 276
2F: Cilia and flagella associated protein 276
2G: Cilia and flagella associated protein 276
2I: Enkurin
2J: Enkurin
2K: Enkurin
2M: Parkin coregulated
2N: Parkin coregulated
2O: Parkin coregulated
2P: Parkin coregulated
2Q: Parkin coregulated
2R: Parkin coregulated
2T: Cilia- and flagella-associated protein 20
2U: Cilia- and flagella-associated protein 20
2V: Cilia- and flagella-associated protein 20
2W: Cilia- and flagella-associated protein 20
2X: Cilia- and flagella-associated protein 20
3A: Protein Flattop
3B: Protein Flattop
3C: Protein Flattop
3E: Tektin
3F: Tektin
3G: Tektin
3H: Tektin
3J: Tektin
3K: Tektin
3L: Tektin
3M: Tektin
3O: Tektin
3P: Tektin
3Q: Tektin
3R: Tektin
3T: Tektin
3U: Tektin
3V: Tektin
3W: Tektin
3Y: RIB43A domain with coiled-coils 2
3Z: RIB43A domain with coiled-coils 2
4A: RIB43A-like with coiled-coils protein 1
4B: RIB43A-like with coiled-coils protein 1
4D: EF-hand domain containing 1
4E: EF-hand domain containing 1
4F: EF-hand domain containing 1
4H: EF-hand domain-containing family member C2
4I: EF-hand domain-containing family member C2
4J: EF-hand domain-containing family member C2
4K: EF-hand domain-containing family member C2
4M: Ciliary microtubule inner protein 2B
4N: Ciliary microtubule inner protein 2B
4O: Ciliary microtubule inner protein 2B
4P: Ciliary microtubule inner protein 2B
4Q: Ciliary microtubule inner protein 2B
4R: Ciliary microtubule inner protein 2B
4T: Sperm-associated antigen 8 isoform 1
4V: Nucleoside diphosphate kinase homolog 7
4W: Nucleoside diphosphate kinase homolog 7
4Y: Cilia and flagella associated protein 161
4Z: Cilia and flagella associated protein 161
5B: Chromosome 1 C9orf135 homolog
5D: Piercer of microtubule wall 1
5E: Piercer of microtubule wall 1
5G: Chromosome 1 C15orf65 homolog
5I: EF-hand domain family member B
5J: EF-hand domain family member B
5L: Cilia and flagella associated protein 141
5N: Meiosis-specific nuclear structural protein 1
5O: Meiosis-specific nuclear structural protein 1
5Q: Cilia- and flagella-associated protein 53
5R: Cilia- and flagella-associated protein 53
5T: CFAP107/C1orf158
5U: CFAP107/C1orf158
5W: Cilia and flagella associated protein 77
5X: Cilia and flagella associated protein 77
5Y: Cilia and flagella associated protein 77
5Z: Cilia and flagella associated protein 77
6A: Cilia and flagella associated protein 144
6C: Cilia-and flagella-associated protein 96
6D: Cilia-and flagella-associated protein 96
6F: Sperm acrosome associated 9
6G: Sperm acrosome associated 9
6H: Sperm acrosome associated 9
6I: Sperm acrosome associated 9
6J: Sperm acrosome associated 9
6K: Sperm acrosome associated 9
6L: Sperm acrosome associated 9
AA: Tubulin alpha chain
AB: Tubulin beta chain
AE: Tubulin alpha chain
AF: Tubulin alpha chain
AG: Tubulin alpha chain
AH: Tubulin alpha chain
AL: Tubulin beta chain
AM: Tubulin beta chain
AN: Tubulin beta chain
AO: Tubulin beta chain
AP: Tubulin beta chain
BA: Tubulin alpha chain
BB: Tubulin beta chain
BE: Tubulin alpha chain
BF: Tubulin alpha chain
BG: Tubulin alpha chain
BH: Tubulin alpha chain
BI: Tubulin alpha chain
BL: Tubulin beta chain
BM: Tubulin beta chain
BN: Tubulin beta chain
BO: Tubulin beta chain
BP: Tubulin beta chain
CA: Tubulin alpha chain
CB: Tubulin beta chain
CE: Tubulin alpha chain
CF: Tubulin alpha chain
CG: Tubulin alpha chain
CH: Tubulin alpha chain
CI: Tubulin alpha chain
CL: Tubulin beta chain
CM: Tubulin beta chain
CN: Tubulin beta chain
CO: Tubulin beta chain
CP: Tubulin beta chain
DA: Tubulin alpha chain
DB: Tubulin beta chain
DE: Tubulin alpha chain
DF: Tubulin alpha chain
DG: Tubulin alpha chain
DH: Tubulin alpha chain
DI: Tubulin alpha chain
DL: Tubulin beta chain
DM: Tubulin beta chain
DN: Tubulin beta chain
DO: Tubulin beta chain
DP: Tubulin beta chain
EA: Tubulin alpha chain
EB: Tubulin beta chain
EE: Tubulin alpha chain
EF: Tubulin alpha chain
EG: Tubulin alpha chain
EH: Tubulin alpha chain
EI: Tubulin alpha chain
EL: Tubulin beta chain
EM: Tubulin beta chain
EN: Tubulin beta chain
EO: Tubulin beta chain
EP: Tubulin beta chain
FA: Tubulin alpha chain
FB: Tubulin beta chain
FE: Tubulin alpha chain
FF: Tubulin alpha chain
FG: Tubulin alpha chain
FH: Tubulin alpha chain
FI: Tubulin alpha chain
FM: Tubulin beta chain
FN: Tubulin beta chain
FO: Tubulin beta chain
FP: Tubulin beta chain
GA: Tubulin alpha chain
GB: Tubulin beta chain
GE: Tubulin alpha chain
GF: Tubulin alpha chain
GG: Tubulin alpha chain
GH: Tubulin alpha chain
GI: Tubulin alpha chain
GM: Tubulin beta chain
GN: Tubulin beta chain
GO: Tubulin beta chain
GP: Tubulin beta chain
HA: Tubulin alpha chain
HB: Tubulin beta chain
HE: Tubulin alpha chain
HF: Tubulin alpha chain
HG: Tubulin alpha chain
HH: Tubulin alpha chain
HI: Tubulin alpha chain
HM: Tubulin beta chain
HN: Tubulin beta chain
HO: Tubulin beta chain
HP: Tubulin beta chain
HQ: Tubulin beta chain
IA: Tubulin alpha chain
IB: Tubulin beta chain
IE: Tubulin alpha chain
IF: Tubulin alpha chain
IG: Tubulin alpha chain
IH: Tubulin alpha chain
II: Tubulin alpha chain
IM: Tubulin beta chain
IN: Tubulin beta chain
IO: Tubulin beta chain
IP: Tubulin beta chain
IQ: Tubulin beta chain
JA: Tubulin alpha chain
JB: Tubulin beta chain
JD: Tubulin alpha chain
JE: Tubulin alpha chain
JF: Tubulin alpha chain
JG: Tubulin alpha chain
JH: Tubulin alpha chain
JL: Tubulin beta chain
JM: Tubulin beta chain
JN: Tubulin beta chain
JO: Tubulin beta chain
KA: Tubulin alpha chain
KB: Tubulin beta chain
KD: Tubulin alpha chain
KE: Tubulin alpha chain
KF: Tubulin alpha chain
KG: Tubulin alpha chain
KH: Tubulin alpha chain
KL: Tubulin beta chain
KM: Tubulin beta chain
KN: Tubulin beta chain
KO: Tubulin beta chain
KP: Tubulin beta chain
LA: Tubulin alpha chain
LB: Tubulin beta chain
LD: Tubulin alpha chain
LE: Tubulin alpha chain
LF: Tubulin alpha chain
LG: Tubulin alpha chain
LH: Tubulin alpha chain
LL: Tubulin beta chain
LM: Tubulin beta chain
LN: Tubulin beta chain
LO: Tubulin beta chain
LP: Tubulin beta chain
MA: Tubulin alpha chain
MB: Tubulin beta chain
MD: Tubulin alpha chain
ME: Tubulin alpha chain
MF: Tubulin alpha chain
MG: Tubulin alpha chain
MH: Tubulin alpha chain
ML: Tubulin beta chain
MM: Tubulin beta chain
MN: Tubulin beta chain
MO: Tubulin beta chain
MP: Tubulin beta chain
NA: Tubulin alpha chain
NB: Tubulin beta chain
ND: Tubulin alpha chain
NE: Tubulin alpha chain
NF: Tubulin alpha chain
NG: Tubulin alpha chain
NH: Tubulin alpha chain
NL: Tubulin beta chain
NM: Tubulin beta chain
NN: Tubulin beta chain
NO: Tubulin beta chain
NP: Tubulin beta chain
OA: Tubulin alpha chain
OB: Tubulin beta chain
OD: Tubulin alpha chain
OE: Tubulin alpha chain
OF: Tubulin alpha chain
OG: Tubulin alpha chain
OH: Tubulin alpha chain
OL: Tubulin beta chain
OM: Tubulin beta chain
ON: Tubulin beta chain
OO: Tubulin beta chain
OP: Tubulin beta chain
PA: Tubulin alpha chain
PB: Tubulin beta chain
PD: Tubulin alpha chain
PE: Tubulin alpha chain
PF: Tubulin alpha chain
PG: Tubulin alpha chain
PH: Tubulin alpha chain
PL: Tubulin beta chain
PM: Tubulin beta chain
PN: Tubulin beta chain
PO: Tubulin beta chain
PP: Tubulin beta chain
QA: Tubulin alpha chain
QB: Tubulin beta chain
QE: Tubulin alpha chain
QF: Tubulin alpha chain
QG: Tubulin alpha chain
QH: Tubulin alpha chain
QL: Tubulin beta chain
QM: Tubulin beta chain
QN: Tubulin beta chain
QO: Tubulin beta chain
QP: Tubulin beta chain
RA: Tubulin alpha chain
RB: Tubulin beta chain
RE: Tubulin alpha chain
RF: Tubulin alpha chain
RG: Tubulin alpha chain
RH: Tubulin alpha chain
RI: Tubulin alpha chain
RL: Tubulin beta chain
RM: Tubulin beta chain
RN: Tubulin beta chain
RO: Tubulin beta chain
RP: Tubulin beta chain
SA: Tubulin alpha chain
SB: Tubulin beta chain
SE: Tubulin alpha chain
SF: Tubulin alpha chain
SG: Tubulin alpha chain
SH: Tubulin alpha chain
SI: Tubulin alpha chain
SL: Tubulin beta chain
SM: Tubulin beta chain
SN: Tubulin beta chain
SO: Tubulin beta chain
SP: Tubulin beta chain
TA: Tubulin alpha chain
TB: Tubulin beta chain
TE: Tubulin alpha chain
TF: Tubulin alpha chain
TG: Tubulin alpha chain
TH: Tubulin alpha chain
TI: Tubulin alpha chain
TL: Tubulin beta chain
TM: Tubulin beta chain
TN: Tubulin beta chain
TO: Tubulin beta chain
TP: Tubulin beta chain
UA: Tubulin alpha chain
UB: Tubulin beta chain
UE: Tubulin alpha chain
UF: Tubulin alpha chain
UG: Tubulin alpha chain
UH: Tubulin alpha chain
UI: Tubulin alpha chain
UM: Tubulin beta chain
UN: Tubulin beta chain
UO: Tubulin beta chain
UP: Tubulin beta chain
VA: Tubulin alpha chain
VB: Tubulin beta chain
VF: Tubulin alpha chain
VG: Tubulin alpha chain
VH: Tubulin alpha chain
VI: Tubulin alpha chain
VJ: Tubulin alpha chain
VN: Tubulin beta chain
VO: Tubulin beta chain
VP: Tubulin beta chain
VQ: Tubulin beta chain
WA: Tubulin alpha chain
WB: Tubulin beta chain
WE: Tubulin alpha chain
WF: Tubulin alpha chain
WG: Tubulin alpha chain
WH: Tubulin alpha chain
WI: Tubulin alpha chain
WM: Tubulin beta chain
WN: Tubulin beta chain
WO: Tubulin beta chain
WP: Tubulin beta chain
WQ: Tubulin beta chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)18,796,804646
Polymers18,666,706377
Non-polymers130,098269
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

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Protein , 23 types, 332 molecules 1A1B1F1G1H1I1J1P1Q2I2J2K2M2N2O2P2Q2R3A3B3C3E3F3G3H3J3K3L3M3O...

#1: Protein Armadillo repeat-containing protein 4 isoform 1 / Outer dynein arm docking complex subunit 2


Mass: 116987.055 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A480NQN9
#3: Protein EF-hand calcium binding domain 1


Mass: 29379.803 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A4X1U4E1
#4: Protein Coiled-coil domain-containing protein 114 isoform X2 / Outer dynein arm docking complex subunit 1


Mass: 80337.516 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: I3LGU2
#6: Protein EFCAB6


Mass: 167103.703 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig)
#11: Protein Enkurin


Mass: 29949.805 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A4X1TZW7
#12: Protein
Parkin coregulated


Mass: 29412.234 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A8D0JJX5
#14: Protein Protein Flattop / Cilia- and flagella-associated protein 126


Mass: 19236.705 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A8D1FAM0
#15: Protein
Tektin


Mass: 48432.867 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A4X1ULV5
#16: Protein
Tektin


Mass: 49973.676 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A8D0ZEV6
#17: Protein
Tektin


Mass: 56552.840 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A4X1SQE5
#18: Protein
Tektin


Mass: 52015.508 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A4X1SYW5
#19: Protein RIB43A domain with coiled-coils 2


Mass: 45003.449 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: F1SM62
#20: Protein RIB43A-like with coiled-coils protein 1


Mass: 44590.852 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A8D0YSQ9
#22: Protein
EF-hand domain-containing family member C2


Mass: 87254.148 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: K7GSU4
#25: Protein Sperm-associated antigen 8 isoform 1


Mass: 50537.250 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A480VJN4
#26: Protein Nucleoside diphosphate kinase homolog 7 / NDK 7


Mass: 42532.766 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A8D0SNF8
#29: Protein Piercer of microtubule wall 1


Mass: 15692.243 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A8D1K3C6
#33: Protein Meiosis-specific nuclear structural protein 1


Mass: 60768.789 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A4X1TJ85
#35: Protein CFAP107/C1orf158


Mass: 23449.852 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A8D1WB49
#38: Protein Cilia-and flagella-associated protein 96


Mass: 34594.367 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A4X1VW16
#39: Protein
Sperm acrosome associated 9


Mass: 25335.152 Da / Num. of mol.: 7 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A8D0ZB69
#40: Protein ...
Tubulin alpha chain


Mass: 49797.035 Da / Num. of mol.: 136 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A5G2QX54
#41: Protein ...
Tubulin beta chain


Mass: 49798.211 Da / Num. of mol.: 133 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A5G2QGK1

-
Outer dynein ... , 2 types, 5 molecules 1C1D1L1M1N

#2: Protein Outer dynein arm-docking complex subunit 4 / Tetratricopeptide repeat protein 25


Mass: 78707.766 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A8D1SCU2
#5: Protein Outer dynein arm docking complex subunit 3


Mass: 72117.602 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A5G2RIE6

-
Cilia- and flagella-associated protein ... , 4 types, 14 molecules 1S1T1U1W1X1Y1Z2T2U2V2W2X5Q5R

#7: Protein Cilia- and flagella-associated protein 52 / WD repeat-containing protein 16


Mass: 68199.891 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: F1SS88
#8: Protein
Cilia- and flagella-associated protein 45


Mass: 65621.914 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A480NP79
#13: Protein
Cilia- and flagella-associated protein 20


Mass: 22781.389 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: F2Z513
#34: Protein Cilia- and flagella-associated protein 53


Mass: 62200.133 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A286ZZD2

-
Cilia and flagella associated protein ... , 6 types, 13 molecules 2B2C2E2F2G4Y4Z5L5W5X5Y5Z6A

#9: Protein Cilia and flagella associated protein 210 / Coiled-coil domain-containing protein 173


Mass: 66292.844 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: F1S1V4
#10: Protein Cilia and flagella associated protein 276


Mass: 19304.631 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A8D0NP25
#27: Protein Cilia and flagella associated protein 161


Mass: 35381.199 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A8D0J8R7
#32: Protein Cilia and flagella associated protein 141


Mass: 12197.253 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A8D0XUD8
#36: Protein
Cilia and flagella associated protein 77


Mass: 32726.729 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: F1S0U2
#37: Protein Cilia and flagella associated protein 144 / Protein FAM183A


Mass: 16325.543 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: F1SFB7

-
EF-hand domain ... , 2 types, 5 molecules 4D4E4F5I5J

#21: Protein EF-hand domain containing 1 / EF-hand domain-containing protein 1 isoform 1 / EF-hand domain-containing protein 1 isoform X1


Mass: 74113.344 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A480I608
#31: Protein EF-hand domain family member B


Mass: 98402.180 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: F1RS61

-
Ciliary microtubule inner protein ... , 2 types, 6 molecules 4M4N4P4Q4R4O

#23: Protein
Ciliary microtubule inner protein 2B


Mass: 30596.564 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A8D1CRM3
#24: Protein Ciliary microtubule inner protein 2B


Mass: 28407.986 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A8D1QC18

-
Chromosome 1 ... , 2 types, 2 molecules 5B5G

#28: Protein Chromosome 1 C9orf135 homolog


Mass: 26817.912 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A8D0NZG3
#30: Protein Chromosome 1 C15orf65 homolog


Mass: 13888.583 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Sus scrofa (pig) / References: UniProt: A0A4X1TH04

-
Non-polymers , 2 types, 269 molecules

#42: Chemical...
ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE


Mass: 523.180 Da / Num. of mol.: 136 / Source method: isolated from a natural source / Formula: C10H16N5O14P3 / Source: (natural) Sus scrofa (pig) / Feature type: SUBJECT OF INVESTIGATION / Comment: GTP, energy-carrying molecule*YM
#43: Chemical...
ChemComp-GDP / GUANOSINE-5'-DIPHOSPHATE


Type: RNA linking / Mass: 443.201 Da / Num. of mol.: 133 / Source method: obtained synthetically / Formula: C10H15N5O11P2 / Feature type: SUBJECT OF INVESTIGATION / Comment: GDP, energy-carrying molecule*YM

-
Details

Has ligand of interestY
Has protein modificationY

-
Experimental details

-
Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: FILAMENT / 3D reconstruction method: single particle reconstruction

-
Sample preparation

ComponentName: cilia from porcine brain ventricles / Type: ORGANELLE OR CELLULAR COMPONENT / Entity ID: #1-#19, #21-#41, #20 / Source: NATURAL
Molecular weightExperimental value: NO
Source (natural)Organism: Sus scrofa (pig)
Buffer solutionpH: 7.4
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

-
Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 2500 nm / Nominal defocus min: 500 nm
Image recordingElectron dose: 50 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k)

-
Processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3.65 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 113809 / Symmetry type: POINT

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