EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	The CRISPR-associated adenosine deaminase Cad1 converts ATP to ITP to provide antiviral immunity.
ジャーナル・号・ページ	Cell, Vol. 187, Issue 25, Page 7183-7195.e24, Year 2024
掲載日	2024年12月12日
著者	Christian F Baca / Puja Majumder / James H Hickling / Linzhi Ye / Marianna Teplova / Sean F Brady / Dinshaw J Patel / Luciano A Marraffini /
PubMed 要旨	Type III CRISPR systems provide immunity against genetic invaders through the production of cyclic oligo-adenylate (cA) molecules that activate effector proteins that contain CRISPR-associated ...Type III CRISPR systems provide immunity against genetic invaders through the production of cyclic oligo-adenylate (cA) molecules that activate effector proteins that contain CRISPR-associated Rossman fold (CARF) domains. Here, we characterized the function and structure of an effector in which the CARF domain is fused to an adenosine deaminase domain, CRISPR-associated adenosine deaminase 1 (Cad1). We show that upon binding of cA or cA to its CARF domain, Cad1 converts ATP to ITP, both in vivo and in vitro. Cryoelectron microscopy (cryo-EM) structural studies on full-length Cad1 reveal an hexameric assembly composed of a trimer of dimers, with bound ATP at inter-domain sites required for activity and ATP/ITP within deaminase active sites. Upon synthesis of cA during phage infection, Cad1 activation leads to a growth arrest of the host that prevents viral propagation. Our findings reveal that CRISPR-Cas systems employ a wide range of molecular mechanisms beyond nucleic acid degradation to provide adaptive immunity in prokaryotes.
リンク	Cell / PubMed:39471810 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.82 - 3.6 Å
構造データ	45241 9c67 EMDB-45241, PDB-9c67: cryoEM structure of CRISPR associated effector, CARF-Adenosine deaminase 1, Cad1, in apo form 手法: EM (単粒子) / 解像度: 3.6 Å 45244 9c6c EMDB-45244, PDB-9c6c: cryoEM structure of CRISPR associated effector, CARF-Adenosine deaminase 1, Cad1, in apo form with ATP (symmetric sites). 手法: EM (単粒子) / 解像度: 3.4 Å 45245 9c6f EMDB-45245, PDB-9c6f: cryoEM structure of CRISPR associated effector, CARF-Adenosine deaminase 1, Cad1, in apo form with ATP (Asymmetric sites). 手法: EM (単粒子) / 解像度: 3.6 Å 45277 9c77 EMDB-45277, PDB-9c77: cryoEM structure of CRISPR associated effector, CARF-Adenosine deaminase 1, Cad1, in cA4 bound form with ATP. 手法: EM (単粒子) / 解像度: 3.2 Å 45466 9cdb EMDB-45466, PDB-9cdb: CryoEM structure of CRISPR associated effector, CARF-Adenosine deaminase 1, Cad1, in cA6 (partial density) bound form with ATP (partial density). 手法: EM (単粒子) / 解像度: 3.6 Å PDB-9c68: The CRISPR associated CARF-adenosine deaminase Cad1-CARF in the cA6 bound form 手法: X-RAY DIFFRACTION / 解像度: 1.82 Å PDB-9c69: The CRISPR associated CARF-adenosine deaminase, Cad1-CARF in the cA4 bound form 手法: X-RAY DIFFRACTION / 解像度: 2.4 Å PDB-9c6a: The CRISPR associated adenosine deaminase Cad1-CARF in the apo form 手法: X-RAY DIFFRACTION / 解像度: 3.6 Å
化合物	ChemComp-MG: Unknown entry ChemComp-HOH: WATER ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, エネルギー貯蔵分子*YM
由来	Bacteriodale bacterium (バクテリア) bacteroidales bacterium (バクテリア) bacteroidale bacteria (バクテリア)
キーワード	ANTIVIRAL PROTEIN / Antiphage defense / CRISPR / Deamination / CARF-Adenosine deaminase / Antiviral Protein/RNA / Type-III CRISPR defense system / CARF-effector protein / adaptive immunity / deamination defense strategy / cyclic Hexa-adenylate / Antiviral Protein-RNA complex / CYTOSOLIC PROTEIN / cyclic Tetra-adenylate / ATP / cA4