EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-electron tomography reveals the binding and release states of the major adhesion complex from Mycoplasma genitalium.
ジャーナル・号・ページ	PLoS Pathog, Vol. 19, Issue 11, Page e1011761, Year 2023
掲載日	2023年11月8日
著者	Lasse Sprankel / Margot P Scheffer / Sina Manger / Utz H Ermel / Achilleas S Frangakis /
PubMed 要旨	The nap particle is an immunogenic surface adhesion complex from Mycoplasma genitalium. It is essential for motility and responsible for binding sialylated oligosaccharides on the surface of the host ...The nap particle is an immunogenic surface adhesion complex from Mycoplasma genitalium. It is essential for motility and responsible for binding sialylated oligosaccharides on the surface of the host cell. The nap particle is composed of two P140-P110 heterodimers, the structure of which was recently solved. However, the interpretation of the mechanism by which the mycoplasma cells orchestrate adhesion remained challenging. Here, we provide cryo-electron tomography structures at ~11 Å resolution, which allow for the distinction between the bound and released state of the nap particle, displaying the in vivo conformational states. Fitting of the atomically resolved structures reveals that bound sialylated oligosaccharides are stabilized by both P110 and P140. Movement of the stalk domains allows for the transfer of conformational changes from the interior of the cell to the binding pocket, thus having the capability of an active release process. It is likely that the same mechanism can be transferred to other Mycoplasma species that belong to the pneumoniae cluster.
リンク	PLoS Pathog / PubMed:37939157 / PubMed Central
手法	EM (単粒子) / EM (サブトモグラム平均)
解像度	3.3 - 18.0 Å
構造データ	17587 8pbx EMDB-17587, PDB-8pbx: Single particle cryo-EM of the P140-P110 heterodimer of Mycoplasma genitalium at 3.3 Angstrom resolution. 手法: EM (単粒子) / 解像度: 3.3 Å 17588 8pby EMDB-17588, PDB-8pby: Single particle cryo-EM of the P140-P110 heterodimer with an alternative conformation in the P140 stalk of Mycoplasma genitalium at a resolution of 3.7 Angstrom. 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-17589: Single particle cryo-EM of the Nap complex of Mycoplasma genitalium in the expanded conformation at 7.3 Angstrom resolution. 手法: EM (単粒子) / 解像度: 7.3 Å EMDB-17590: Single particle cryo-EM of the Nap adhesion complex of Mycoplasma genitalium in the canonical conformation at 8.3 Angstrom resolution. 手法: EM (単粒子) / 解像度: 8.3 Å 17591 8pbz EMDB-17591, PDB-8pbz: Sub-tomogram average of the Nap adhesion complex from the human pathogen Mycoplasma genitalium at 11 Angstrom. 手法: EM (サブトモグラム平均) / 解像度: 11.0 Å 17592 8pc0 EMDB-17592, PDB-8pc0: Sub-tomogram average of the open conformation of the Nap adhesion complex from the human pathogen Mycoplasma genitalium. 手法: EM (サブトモグラム平均) / 解像度: 17.0 Å 17593 8pc1 EMDB-17593, PDB-8pc1: Sub-tomogram average of the closed conformation of the Nap adhesion complex from the human pathogen Mycoplasma genitalium. 手法: EM (サブトモグラム平均) / 解像度: 18.0 Å EMDB-18414: Single particle cryo-EM of the Nap adhesion complex of Mycoplasma genitalium soaked with 6'-SL at 7.7 Angstrom resolution. 手法: EM (単粒子) / 解像度: 7.7 Å
化合物	ChemComp-K: Unknown entry / カリウムカチオン ChemComp-PO4: PHOSPHATE ION / ホスファ-ト ChemComp-HOH: WATER
由来	mycoplasmoides genitalium g37 (バクテリア)
キーワード	CELL ADHESION / Adhesion / Mycoplasma genitalium