EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Molecular basis of cholesterol efflux via ABCG subfamily transporters.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 118, Issue 34, Year 2021
掲載日	2021年8月24日
著者	Yingyuan Sun / Jin Wang / Tao Long / Xiaofeng Qi / Linda Donnelly / Nadia Elghobashi-Meinhardt / Leticia Esparza / Jonathan C Cohen / Xiao-Song Xie / Helen H Hobbs / Xiaochun Li /
PubMed 要旨	The ABCG1 homodimer (G1) and ABCG5-ABCG8 heterodimer (G5G8), two members of the adenosine triphosphate (ATP)-binding cassette (ABC) transporter G family, are required for maintenance of cellular ...The ABCG1 homodimer (G1) and ABCG5-ABCG8 heterodimer (G5G8), two members of the adenosine triphosphate (ATP)-binding cassette (ABC) transporter G family, are required for maintenance of cellular cholesterol levels. G5G8 mediates secretion of neutral sterols into bile and the gut lumen, whereas G1 transports cholesterol from macrophages to high-density lipoproteins (HDLs). The mechanisms used by G5G8 and G1 to recognize and export sterols remain unclear. Here, we report cryoelectron microscopy (cryo-EM) structures of human G5G8 in sterol-bound and human G1 in cholesterol- and ATP-bound states. Both transporters have a sterol-binding site that is accessible from the cytosolic leaflet. A second site is present midway through the transmembrane domains of G5G8. The Walker A motif of G8 adopts a unique conformation that accounts for the marked asymmetry in ATPase activities between the two nucleotide-binding sites of G5G8. These structures, along with functional validation studies, provide a mechanistic framework for understanding cholesterol efflux via ABC transporters.
リンク	Proc Natl Acad Sci U S A / PubMed:34404721 / PubMed Central
手法	EM (単粒子)
解像度	2.6 - 3.7 Å
構造データ	24310 7r87 EMDB-24310, PDB-7r87: The structure of human ABCG5-WT/ABCG8-I419E 手法: EM (単粒子) / 解像度: 3.4 Å 24311 7r88 EMDB-24311, PDB-7r88: The structure of human ABCG5-I529W/ABCG8-WT 手法: EM (単粒子) / 解像度: 3.5 Å 24312 7r89 EMDB-24312, PDB-7r89: The structure of human ABCG5/ABCG8 purified from yeast 手法: EM (単粒子) / 解像度: 2.6 Å 24313 7r8a EMDB-24313, PDB-7r8a: The structure of human ABCG5/ABCG8 purified from mammalian cells 手法: EM (単粒子) / 解像度: 2.9 Å 24314 7r8b EMDB-24314, PDB-7r8b: The structure of human ABCG5/ABCG8 supplemented with cholesterol 手法: EM (単粒子) / 解像度: 3.1 Å 24315 7r8c EMDB-24315, PDB-7r8c: The structure of human ABCG1 手法: EM (単粒子) / 解像度: 3.7 Å 24316 7r8d EMDB-24316, PDB-7r8d: The structure of human ABCG1 E242Q with cholesterol 手法: EM (単粒子) / 解像度: 3.2 Å 24317 7r8e EMDB-24317, PDB-7r8e: The structure of human ABCG1 E242Q complexed with ATP 手法: EM (単粒子) / 解像度: 3.7 Å
化合物	ChemComp-ERG: ERGOSTEROL / エルゴスタ-5,7,22-トリエン-3β-オ-ル ChemComp-CLR: CHOLESTEROL / コレステロ-ル ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-MG*: Unknown entry / マグネシウムジカチオン
由来	homo sapiens (ヒト) mus musculus (ハツカネズミ)
キーワード	LIPID TRANSPORT/IMMUNE SYSTEM / sterol / lipids / ABC transporter / LIPID TRANSPORT / LIPID TRANSPORT-IMMUNE SYSTEM complex