EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Nucleolar maturation of the human small subunit processome.
ジャーナル・号・ページ	Science, Vol. 373, Issue 6560, Page eabj5338, Year 2021
掲載日	2021年9月10日
著者	Sameer Singh / Arnaud Vanden Broeck / Linamarie Miller / Malik Chaker-Margot / Sebastian Klinge /
PubMed 要旨	The human small subunit processome mediates early maturation of the small ribosomal subunit by coupling RNA folding to subsequent RNA cleavage and processing steps. We report the high-resolution ...The human small subunit processome mediates early maturation of the small ribosomal subunit by coupling RNA folding to subsequent RNA cleavage and processing steps. We report the high-resolution cryo–electron microscopy structures of maturing human small subunit (SSU) processomes at resolutions of 2.7 to 3.9 angstroms. These structures reveal the molecular mechanisms that enable crucial progressions during SSU processome maturation. RNA folding states within these particles are communicated to and coordinated with key enzymes that drive irreversible steps such as targeted exosome-mediated RNA degradation, protein-guided site-specific endonucleolytic RNA cleavage, and tightly controlled RNA unwinding. These conserved mechanisms highlight the SSU processome’s impressive structural plasticity, which endows this 4.5-megadalton nucleolar assembly with the distinctive ability to mature the small ribosomal subunit from within.
リンク	Science / PubMed:34516797 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.23 - 6.37 Å
構造データ	23936 7mq8 EMDB-23936, PDB-7mq8: Cryo-EM structure of the human SSU processome, state pre-A1 手法: EM (単粒子) / 解像度: 3.6 Å 23937 7mq9 EMDB-23937, PDB-7mq9: Cryo-EM structure of the human SSU processome, state pre-A1* 手法: EM (単粒子) / 解像度: 3.87 Å 23938 7mqa EMDB-23938, PDB-7mqa: Cryo-EM structure of the human SSU processome, state post-A1 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-23939: Cryo-EM structure of the human SSU processome, state pre-A1 - raw maps 手法: EM (単粒子) / 解像度: 3.51 Å EMDB-23940: Cryo-EM structure of the human SSU processome, state post-A1 - raw maps 手法: EM (単粒子) / 解像度: 2.65 Å EMDB-24149: Cryo-EM structure of the human SSU processome, state pre-A1 - 5'ETS focused map 手法: EM (単粒子) / 解像度: 3.73 Å EMDB-24150: Cryo-EM structure of the human SSU processome, state pre-A1 - Core focused map 手法: EM (単粒子) / 解像度: 3.29 Å EMDB-24151: Cryo-EM structure of the human SSU processome, state pre-A1 - HEATR1 focused map 手法: EM (単粒子) / 解像度: 6.37 Å EMDB-24152: Cryo-EM structure of the human SSU processome, state pre-A1 - NAT10-NOL10 focused map 手法: EM (単粒子) / 解像度: 3.67 Å EMDB-24153: Cryo-EM structure of the human SSU processome, state pre-A1 - NEP1 focused map 手法: EM (単粒子) / 解像度: 3.54 Å EMDB-24154: Cryo-EM structure of the human SSU processome, state pre-A1 - NGDN focused map 手法: EM (単粒子) / 解像度: 3.83 Å EMDB-24155: Cryo-EM structure of the human SSU processome, state pre-A1 - PNO1 focused map 手法: EM (単粒子) / 解像度: 4.06 Å EMDB-24156: Cryo-EM structure of the human SSU processome, state pre-A1 - TBL3 focused map 手法: EM (単粒子) / 解像度: 4.09 Å EMDB-24157: Cryo-EM structure of the human SSU processome, state pre-A1 - UTP20-CTD focused map 手法: EM (単粒子) / 解像度: 6.3 Å EMDB-24158: Cryo-EM structure of the human SSU processome, state pre-A1 - UTP20-NTD focused map 手法: EM (単粒子) / 解像度: 3.42 Å EMDB-24159: Cryo-EM structure of the human SSU processome, state pre-A1 - UTPA focused map 手法: EM (単粒子) / 解像度: 3.58 Å EMDB-24160: Cryo-EM structure of the human SSU processome, state pre-A1 - UTPC focused map 手法: EM (単粒子) / 解像度: 4.45 Å EMDB-24161: Cryo-EM structure of the human SSU processome, state pre-A1 - UTPC-Central focused map 手法: EM (単粒子) / 解像度: 3.73 Å EMDB-24162: Cryo-EM structure of the human SSU processome, state post-A1 - Core focused map 手法: EM (単粒子) / 解像度: 2.63 Å EMDB-24163: Cryo-EM structure of the human SSU processome, state post-A1 - DHX37 focused map 手法: EM (単粒子) / 解像度: 4.23 Å EMDB-24164: Cryo-EM structure of the human SSU processome, state post-A1 - H44 focused map 手法: EM (単粒子) / 解像度: 3.87 Å EMDB-24165: Cryo-EM structure of the human SSU processome, state post-A1 - HEATR1 focused map 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-24166: Cryo-EM structure of the human SSU processome, state post-A1 - probable MYBBP1A focused map 手法: EM (単粒子) / 解像度: 3.71 Å EMDB-24167: Cryo-EM structure of the human SSU processome, state post-A1 - NOL11 focused map 手法: EM (単粒子) / 解像度: 3.57 Å EMDB-24168: Cryo-EM structure of the human SSU processome, state post-A1 - RPS19-AROS focused map 手法: EM (単粒子) / 解像度: 2.99 Å EMDB-24169: Cryo-EM structure of the human SSU processome, state post-A1 - UTP20 focused map 手法: EM (単粒子) / 解像度: 2.72 Å EMDB-24170: Cryo-EM structure of the human SSU processome, state post-A1 - UTP6 focused map 手法: EM (単粒子) / 解像度: 2.96 Å EMDB-24171: Cryo-EM structure of the human SSU processome, state post-A1 - UTPA focused map 手法: EM (単粒子) / 解像度: 2.78 Å EMDB-24172: Cryo-EM structure of the human SSU processome, state post-A1 - UTPC focused map 手法: EM (単粒子) / 解像度: 2.89 Å EMDB-24173: Cryo-EM structure of the human SSU processome, state post-A1 - UTPC-central focused map 手法: EM (単粒子) / 解像度: 2.6 Å EMDB-24174: Cryo-EM structure of the human SSU processome - BYST-RPS12 focused map 手法: EM (単粒子) / 解像度: 3.46 Å EMDB-24175: Cryo-EM structure of the human SSU processome - NOC4L-NOP14 focused map 手法: EM (単粒子) / 解像度: 3.41 Å EMDB-24176: Cryo-EM structure of the human SSU processome - RRP12-BYST focused map 手法: EM (単粒子) / 解像度: 5.25 Å PDB-7mqj: Dhr1 Helicase Core 手法: X-RAY DIFFRACTION / 解像度: 2.23 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-ZN: Unknown entry ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM / グアノシン三リン酸 ChemComp-SAH: S-ADENOSYL-L-HOMOCYSTEINE / S-アデノシルホモシステイン / S-アデノシル-L-ホモシステイン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-HOH*: WATER / 水
由来	homo sapiens (ヒト) Human (ヒト) saccharomyces cerevisiae (パン酵母)
キーワード	RIBOSOME (リボソーム) / Ribosomal assembly intermediate / HYDROLASE (加水分解酵素) / Helicase (ヘリカーゼ) / Ribosome Assembly (リボソーム)