タイトル | A structural and kinetic survey of GH5_4 endoglucanases reveals determinants of broad substrate specificity and opportunities for biomass hydrolysis. |
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ジャーナル・号・ページ | J. Biol. Chem., Vol. 295, Page 17752-17769, Year 2020 |
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掲載日 | 2013年1月2日 (構造データの登録日) |
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著者 | Glasgow, E.M. / Kemna, E.I. / Bingman, C.A. / Ing, N. / Deng, K. / Bianchetti, C.M. / Takasuka, T.E. / Northen, T.R. / Fox, B.G. |
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リンク | J. Biol. Chem. / PubMed:33454012 |
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手法 | X線回折 |
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解像度 | 1.05 - 2.42 Å |
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構造データ | PDB-4im4: Multifunctional cellulase, xylanase, mannanase 手法: X-RAY DIFFRACTION / 解像度: 2.42 Å PDB-6mq4: GH5-4 broad specificity endoglucanase from Hungateiclostridium cellulolyticum 手法: X-RAY DIFFRACTION / 解像度: 1.4 Å PDB-6pz7: GH5-4 broad specificity endoglucanase from Clostridium acetobutylicum 手法: X-RAY DIFFRACTION / 解像度: 1.25 Å PDB-6q1i: GH5-4 broad specificity endoglucanase from Clostrdium longisporum 手法: X-RAY DIFFRACTION / 解像度: 1.35 Å PDB-6ui3: GH5-4 broad specificity endoglucanase from Clostridum cellulovorans 手法: X-RAY DIFFRACTION / 解像度: 1.3 Å PDB-6wqp: GH5-4 broad specificity endoglucanase from Ruminococcus champanellensis 手法: X-RAY DIFFRACTION / 解像度: 1.6 Å PDB-6wqv: GH5-4 broad specificity endoglucanase from Ruminococcus champanellensis with bound cellotriose 手法: X-RAY DIFFRACTION / 解像度: 1.45 Å PDB-6wqy: GH5-4 broad specificity endoglucanase from Bacteroides salanitronis 手法: X-RAY DIFFRACTION / 解像度: 1.05 Å PDB-6xrk: GH5-4 broad specificity endoglucanase from an uncultured bovine rumen ciliate 手法: X-RAY DIFFRACTION / 解像度: 1.419 Å PDB-6xso: GH5-4 broad specificity endoglucanase from an uncultured bacterium 手法: X-RAY DIFFRACTION / 解像度: 1.5 Å PDB-6xsu: GH5-4 broad specificity endoglucanase from Ruminococcus flavefaciens 手法: X-RAY DIFFRACTION / 解像度: 1.41 Å |
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化合物 | ChemComp-BTB: 2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / ビストリス / pH緩衝剤*YM
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由来 | clostridium thermocellum (バクテリア) hungateiclostridium cellulolyticum (バクテリア) clostridium acetobutylicum atcc 824 (バクテリア) clostridium longisporum (バクテリア) clostridium cellulovorans (バクテリア) ruminococcus champanellensis (バクテリア) bacteroides salanitronis (strain dsm 18170 / jcm 13567 / bl78) (バクテリア) uncultured bovine rumen ciliate (環境試料) uncultured bacterium (環境試料) ruminococcus flavefaciens (バクテリア)
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キーワード | HYDROLASE / cellulase / xylanase / mannanase / multifunction / Endo-1 / 4-beta-glucanase / biomass degradation / manganese / GH5 / endoglucanase / GH5-4 / GLBRC |
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