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- PDB-6q1i: GH5-4 broad specificity endoglucanase from Clostrdium longisporum -
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Open data
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Basic information
Entry | Database: PDB / ID: 6q1i | ||||||
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Title | GH5-4 broad specificity endoglucanase from Clostrdium longisporum | ||||||
![]() | Endoglucanase A | ||||||
![]() | HYDROLASE / GH5-4 / endoglucanase / GLBRC | ||||||
Function / homology | ![]() cellulase / polysaccharide binding / cellulase activity / cellulose catabolic process Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Bianchetti, C.M. / Bingman, C.A. / Fox, B.G. | ||||||
Funding support | ![]()
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![]() | ![]() Title: A structural and kinetic survey of GH5_4 endoglucanases reveals determinants of broad substrate specificity and opportunities for biomass hydrolysis. Authors: Glasgow, E.M. / Kemna, E.I. / Bingman, C.A. / Ing, N. / Deng, K. / Bianchetti, C.M. / Takasuka, T.E. / Northen, T.R. / Fox, B.G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 509.1 KB | Display | ![]() |
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PDB format | ![]() | 349.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 423.1 KB | Display | ![]() |
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Full document | ![]() | 424.3 KB | Display | |
Data in XML | ![]() | 33.1 KB | Display | |
Data in CIF | ![]() | 52.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4im4C ![]() 6mq4C ![]() 6pz7C ![]() 6ui3C ![]() 6wqpC ![]() 6wqvC ![]() 6wqyC ![]() 6xrkC ![]() 6xsoC ![]() 6xsuC ![]() 3ndyS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 40055.492 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.03 Å3/Da / Density % sol: 39.35 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: Crystals were set with a Mosquito into SD2 plates. The condition that provided the crystals for this data set came from Morpheus Screen D9: 10% Alcohols, 50% Tris/Bicine H 8.5, and 15% P550MME_P20K |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Oct 13, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97856 Å / Relative weight: 1 |
Reflection | Resolution: 1.35→25.21 Å / Num. obs: 128382 / % possible obs: 94.83 % / Redundancy: 3.8 % / Biso Wilson estimate: 10.73 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.04018 / Rpim(I) all: 0.02412 / Rrim(I) all: 0.04695 / Net I/σ(I): 20.86 |
Reflection shell | Resolution: 1.35→1.398 Å / Rmerge(I) obs: 0.1076 / Mean I/σ(I) obs: 8.28 / Num. unique obs: 12528 / CC1/2: 0.988 / Rpim(I) all: 0.07146 / Rrim(I) all: 0.13 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3NDY Resolution: 1.35→25.21 Å / SU ML: 0.0975 / Cross valid method: FREE R-VALUE / σ(F): 2.03 / Phase error: 13.5275
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 14.77 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.35→25.21 Å
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Refine LS restraints |
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LS refinement shell |
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