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-Structure paper
タイトル | Cryo-EM analyses reveal the common mechanism and diversification in the activation of RET by different ligands. |
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ジャーナル・号・ページ | Elife, Vol. 8, Year 2019 |
掲載日 | 2019年9月19日 |
著者 | Jie Li / Guijun Shang / Yu-Ju Chen / Chad A Brautigam / Jen Liou / Xuewu Zhang / Xiao-Chen Bai / |
PubMed 要旨 | RET is a receptor tyrosine kinase (RTK) that plays essential roles in development and has been implicated in several human diseases. Different from most of RTKs, RET requires not only its cognate ...RET is a receptor tyrosine kinase (RTK) that plays essential roles in development and has been implicated in several human diseases. Different from most of RTKs, RET requires not only its cognate ligands but also co-receptors for activation, the mechanisms of which remain unclear due to lack of high-resolution structures of the ligand/co-receptor/receptor complexes. Here, we report cryo-EM structures of the extracellular region ternary complexes of GDF15/GFRAL/RET, GDNF/GFRα1/RET, NRTN/GFRα2/RET and ARTN/GFRα3/RET. These structures reveal that all the four ligand/co-receptor pairs, while using different atomic interactions, induce a specific dimerization mode of RET that is poised to bring the two kinase domains into close proximity for cross-phosphorylation. The NRTN/GFRα2/RET dimeric complex further pack into a tetrameric assembly, which is shown by our cell-based assays to regulate the endocytosis of RET. Our analyses therefore reveal both the common mechanism and diversification in the activation of RET by different ligands. |
リンク | Elife / PubMed:31535977 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.4 - 4.4 Å |
構造データ | EMDB-20572, PDB-6q2j: EMDB-20573: EMDB-20575, PDB-6q2n: EMDB-20576, PDB-6q2o: EMDB-20577: EMDB-20578, PDB-6q2r: EMDB-20579, PDB-6q2s: EMDB-20580: |
化合物 | ChemComp-NAG: ChemComp-CA: |
由来 |
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キーワード | SIGNALING PROTEIN / RET / receptor tyrosine kinase / cryo-EM |